################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I AspEI AspI AviII AvrII BalI BbeI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaI BsaWI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HphI KasI MaeII MfeI MluI MluNI MroI MscI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuII RcaI RsrII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tsp45I Tth111I Van91I XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 840 GGTACC G^GTACC AcyI 238 GACGCC G[GA]^CG[CT]C AlwNI 848 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 691 ATTAAT AT^TAAT AsnI 691 ATTAAT AT^TAAT Asp718I 840 GGTACC G^GTACC AvaII 342 GGACC G^G[AT]CC BamHI 273 GGATCC G^GATCC BanI 840 GGTACC G^G[CT][GA]CC BanII 145 GAGCTC G[GA]GC[CT]^C BbvCI 255 CCTCAGC CCTCAGC(-5/-2) BpmI 854 CTGGAG CTGGAG(16/14) BsaBI 274 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 238 GACGCC G[GA]^CG[CT]C BsgI 862 GTGCAG GTGCAG(16/14) BsiEI 643 CGATCG CG[GA][CT]^CG BsmFI 341 GGGAC GGGAC(10/14) BsrDI 165 GCAATG GCAATG(2/0) BsrFI 344 ACCGGC [GA]^CCGG[CT] BstAPI 603 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cac8I 260 GCACGC GC[ACGT]^[ACGT]GC Cfr10I 344 ACCGGC [GA]^CCGG[CT] DraI 420 TTTAAA TTT^AAA DraII 849 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoNI 483 CCTCAATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 849 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoT14I 835 CCATGG C^C[AT][AT]GG HaeIII 850 GGCC GG^CC HgaI 238 GACGC GACGC(5/10) Hin1I 238 GACGCC G[GA]^CG[CT]C HincII 157 GTTAAC GT[CT]^[GA]AC HindII 157 GTTAAC GT[CT]^[GA]AC HindIII 740 AAGCTT A^AGCTT HinfI 251 GATTC G^A[ACGT]TC HpaI 157 GTTAAC GTT^AAC KpnI 840 GGTACC GGTAC^C KspI 242 CCGCGG CCGC^GG MaeIII 803 GTAAC ^GT[ACGT]AC MamI 274 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 835 CCATGG C^CATGG NruI 327 TCGCGA TCG^CGA PaeR7I 551 CTCGAG C^TCGAG PshBI 691 ATTAAT AT^TAAT PstI 349 CTGCAG CTGCA^G PvuI 643 CGATCG CGAT^CG SacI 145 GAGCTC GAGCT^C SacII 242 CCGCGG CCGC^GG SapI 493 GCTCTTC GCTCTTC(1/4) SmaI 338 CCCGGG CCC^GGG SmlI 551 CTCGAG C^T[CT][GA]AG StyI 835 CCATGG C^C[AT][AT]GG TfiI 251 GATTC G^A[AT]TC TspRI 895 CAGTG CA[GC]TG[ACGT][ACGT]^ XbaI 25 TCTAGA T^CTAGA XcmI 886 CCAGACTATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 551 CTCGAG C^TCGAG XmaCI 338 CCCGGG C^CCGGG XmaI 338 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AspHI 145 GAGCTC G[AT]GC[AT]^C 877 GAGCAC AvaI 338 CCCGGG C^[CT]CG[GA]G 551 CTCGAG BbsI 225 GAAGAC GAAGAC(2/6) 625 GAAGAC BcnI 338 CCCGG CC^[GC]GG 339 CCGGG BfaI 26 CTAG C^TAG 766 CTAG BpuAI 225 GAAGAC GAAGAC(2/6) 625 GAAGAC BsiHKAI 145 GAGCTC G[AT]GC[AT]^C 877 GAGCAC BsmAI 657 GAGAC GTCTC(1/5) 549 GTCTC BsoBI 338 CCCGGG C^[CT]CG[GA]G 551 CTCGAG Bsp1407I 12 TGTACA T^GTACA 927 TGTACA BspMI 824 ACCTGC ACCTGC(4/8) 606 GCAGGT BsrGI 12 TGTACA T^GTACA 927 TGTACA BsrI 234 ACTGG ACTGG(1/-1) 711 CCAGT BstF5I 970 CATCC GGATG(2/0) 501 GGATG BstNI 150 CCTGG CC^[AT]GG 853 CCTGG BstYI 273 GGATCC [GA]^GATC[CT] 831 AGATCC Cfr13I 342 GGACC G^G[ACGT]CC 850 GGCCC DdeI 256 CTCAG C^T[ACGT]AG 954 CTCAG DsaI 242 CCGCGG C^C[GA][CT]GG 835 CCATGG EarI 494 CTCTTC CTCTTC(1/4) 406 GAAGAG EcoRII 150 CCTGG ^CC[AT]GG 853 CCTGG FauI 264 GCGGG CCCGC(4/6) 354 GCGGG FokI 970 CATCC GGATG(9/13) 501 GGATG HapII 339 CCGG C^CGG 345 CCGG HpaII 339 CCGG C^CGG 345 CCGG Ksp632I 494 CTCTTC CTCTTC(1/4) 406 GAAGAG MaeI 26 CTAG C^TAG 766 CTAG MflI 273 GGATCC [GA]^GATC[CT] 831 AGATCC MspA1I 242 CCGCGG C[AC]G^C[GT]G 352 CAGCGG MspI 339 CCGG C^CGG 345 CCGG MvaI 150 CCTGG CC^[AT]GG 853 CCTGG NciI 338 CCCGG CC^[GC]GG 339 CCGGG Sau96I 342 GGACC G^G[ACGT]CC 850 GGCCC SfcI 349 CTGCAG C^T[GA][CT]AG 682 CTACAG SspBI 12 TGTACA T^GTACA 927 TGTACA TaqI 552 TCGA T^CGA 651 TCGA TthHB8I 552 TCGA T^CGA 651 TCGA XhoII 273 GGATCC [GA]^GATC[CT] 831 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI BbvI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BssKI BstUI DpnII EcoRI Fnu4HI ItaI MboI MboII MnlI MseI MvnI MwoI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI SfaNI Tru9I TseI Tsp509I