(Circular) (Six-base) MAPSORT of: pFN21AE5353.seq Check: 4969 from: 1 to: 5680 pFN21AE5353 5680 bp With 182 enzymes: SgfI * January 30, 2014 17:18 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2602 2838 2602 Size: 236 5444 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3490 278 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3023 1937 Size: 1086 4594 Acc65I G'GTAC_C Cuts at: 2845 3001 2845 Size: 156 5524 AclI AA'CG_TT Cuts at: 1563 3807 4180 1563 Size: 2244 373 3063 Fragments arranged by size: 3063 2244 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2249 2486 2624 3743 5127 5395 1632 Size: 617 237 138 1119 1384 268 1917 Fragments arranged by size: 1917 1384 1119 617 268 237 138 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5483 AflIII A'CryG_T Cuts at: 1203 4579 5556 1203 Size: 3376 977 1327 Fragments arranged by size: 3376 1327 977 AgeI A'CCGG_T Cuts at: 4511 4511 Size: 5680 AhdI GACnn_n'nnGTC Cuts at: 4409 4409 Size: 5680 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5680 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5680 AlwNI CAG_nnn'CTG Cuts at: 2858 3423 4995 2858 Size: 565 1572 3543 Fragments arranged by size: 3543 1572 565 ApaLI G'TGCA_C Cuts at: 3737 4893 3737 Size: 1156 4524 ApoI r'AATT_y Cuts at: 1493 2104 2564 2731 2989 3215 3367 5476 1493 Size: 611 460 167 258 226 152 2109 1697 Fragments arranged by size: 2109 1697 611 460 258 226 167 152 AseI AT'TA_AT Cuts at: 160 1032 2697 4234 160 Size: 872 1665 1537 1606 Fragments arranged by size: 1665 1606 1537 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 AvaI C'yCGr_G Cuts at: 1870 1943 2343 2556 3005 1870 Size: 73 400 213 449 4545 Fragments arranged by size: 4545 449 400 213 73 BamHI G'GATC_C Cuts at: 2278 3010 3377 2278 Size: 732 367 4581 Fragments arranged by size: 4581 732 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2845 3001 4456 618 Size: 325 217 627 1058 156 1455 1842 Fragments arranged by size: 1842 1455 1058 627 325 217 156 BanII G_rGCy'C Cuts at: 1391 2154 2999 1391 Size: 763 845 4072 Fragments arranged by size: 4072 845 763 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2237 2637 928 Size: 971 338 400 3971 Fragments arranged by size: 3971 971 400 338 BbvCI CC'TCA_GC Cuts at: 2261 2261 Size: 5680 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3871 3905 1903 Size: 34 1934 34 3678 Fragments arranged by size: 3678 1934 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3572 4782 3572 Size: 1210 4470 BclI T'GATC_A Cuts at: 1548 1548 Size: 5680 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4291 136 Size: 107 122 71 631 605 2619 1525 Fragments arranged by size: 2619 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5675 5675 Size: 5680 BlpI GC'TnA_GC Cuts at: 3097 3097 Size: 5680 Bme1580I G_kGCm'C Cuts at: 1790 2027 3741 4897 1790 Size: 237 1714 1156 2573 Fragments arranged by size: 2573 1714 1156 237 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5680 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2710 3411 4369 Size: 416 53 83 399 24 1293 701 958 Cuts at: 4369 442 Size: 1753 Fragments arranged by size: 1753 1293 958 701 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5680 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2571 2571 Size: 5680 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2880 4340 1239 Size: 1641 1460 2579 Fragments arranged by size: 2579 1641 1460 Bpu10I CC'TnA_GC Cuts at: 2261 2261 Size: 5680 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3805 4670 4968 3805 Size: 865 298 4517 Fragments arranged by size: 4517 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4343 882 Size: 617 2844 2219 Fragments arranged by size: 2844 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3471 493 Size: 711 2267 2702 Fragments arranged by size: 2702 2267 711 BsaBI GATnn'nnATC Cuts at: 2283 5674 2283 Size: 3391 2289 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2244 3487 3869 4504 Size: 53 83 186 936 711 1243 382 635 Cuts at: 4504 275 Size: 1451 Fragments arranged by size: 1451 1243 936 711 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2977 4113 4511 4785 4932 5343 2977 Size: 1136 398 274 147 411 3314 Fragments arranged by size: 3314 1136 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5455 Fragments arranged by size: 5455 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2378 2591 2696 1186 Size: 1192 213 105 4170 Fragments arranged by size: 4170 1192 213 105 BseYI C'CCAG_C Cuts at: 4883 5379 4883 Size: 496 5184 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2888 1163 Size: 573 1152 3955 Fragments arranged by size: 3955 1152 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2651 3130 3891 4919 1540 Size: 275 186 650 479 761 1028 2301 Fragments arranged by size: 2301 1028 761 650 479 275 186 BsiHKAI G_wGCw'C Cuts at: 2154 2886 3741 3826 4897 2154 Size: 732 855 85 1071 2937 Fragments arranged by size: 2937 1071 855 732 85 BsmI GAATG_Cn' Cuts at: 3191 3284 3191 Size: 93 5587 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2027 2154 2886 2999 3741 3826 4897 Size: 399 237 127 732 113 742 85 1071 Cuts at: 4897 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 732 399 237 127 113 85 BspEI T'CCGG_A Cuts at: 5343 5343 Size: 5680 BspHI T'CATG_A Cuts at: 3569 5299 3569 Size: 1730 3950 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2602 2838 3035 844 Size: 1758 236 197 3489 Fragments arranged by size: 3489 1758 236 197 BsrBI CCG'CTC Cuts at: 1394 1823 3567 1394 Size: 429 1744 3507 Fragments arranged by size: 3507 1744 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2177 4175 4349 65 Size: 1183 929 1998 174 1396 Fragments arranged by size: 1998 1396 1183 929 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2349 4324 4511 1700 Size: 110 539 1975 187 2869 Fragments arranged by size: 2869 1975 539 187 110 BsrGI T'GTAC_A Cuts at: 96 2017 2932 96 Size: 1921 915 2844 Fragments arranged by size: 2844 1921 915 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5680 BssSI C'ACGA_G Cuts at: 3740 4752 3740 Size: 1012 4668 BstAPI GCAn_nnn'nTGC Cuts at: 794 2614 794 Size: 1820 3860 BstBI TT'CG_AA Cuts at: 4484 4484 Size: 5680 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5680 BstYI r'GATC_y Cuts at: 1972 2278 2836 3010 3377 3762 3779 5231 5317 Size: 306 558 174 367 385 17 1452 86 Cuts at: 5317 5329 5675 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 558 385 367 346 306 174 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2247 2840 513 Size: 552 939 243 593 3353 Fragments arranged by size: 3353 939 593 552 243 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2315 487 Size: 683 56 1089 3852 Fragments arranged by size: 3852 1089 683 56 BtsI GCAGTG_nn' Cuts at: 3196 3990 4010 3196 Size: 794 20 4866 Fragments arranged by size: 4866 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5680 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5645 DraI TTT'AAA Cuts at: 2427 2984 2427 Size: 557 5123 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4687 809 Size: 507 3371 1802 Fragments arranged by size: 3371 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3127 4016 5645 Size: 54 1425 50 275 18 1297 889 1629 Cuts at: 5645 8 Size: 43 Fragments arranged by size: 1629 1425 1297 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3127 1537 Size: 275 1315 4090 Fragments arranged by size: 4090 1315 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2406 2505 3610 1353 Size: 1053 99 1105 3423 Fragments arranged by size: 3423 1105 1053 99 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2913 4267 4641 4787 387 Size: 1064 138 1324 1354 374 146 1280 Fragments arranged by size: 1354 1324 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2152 2152 Size: 5680 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 2486 2624 2880 3743 4340 5127 Size: 393 617 237 138 256 863 597 787 Cuts at: 5127 5395 1239 Size: 268 1524 Fragments arranged by size: 1524 863 787 617 597 393 268 256 237 138 EcoNI CCTnn'n_nnAGG Cuts at: 2492 2492 Size: 5680 EcoO109I rG'GnC_Cy Cuts at: 2855 2855 Size: 5680 EcoRI G'AATT_C Cuts at: 2104 2564 2731 2989 3367 2104 Size: 460 167 258 378 4417 Fragments arranged by size: 4417 460 378 258 167 FspI TGC'GCA Cuts at: 4186 4186 Size: 5680 HaeII r_GCGC'y Cuts at: 1678 4827 1678 Size: 3149 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2092 2124 2653 2685 4321 Size: 32 135 32 520 32 529 32 1636 Cuts at: 4321 4353 4395 4427 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1636 529 520 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2164 3024 3270 669 Size: 988 281 226 860 246 3079 Fragments arranged by size: 3079 988 860 281 246 226 HindIII A'AGCT_T Cuts at: 748 2745 748 Size: 1997 3683 HpaI GTT'AAC Cuts at: 2164 3270 2164 Size: 1106 4574 KpnI G_GTAC'C Cuts at: 2849 3005 2849 Size: 156 5524 MfeI C'AATT_G Cuts at: 3279 3279 Size: 5680 MluI A'CGCG_T Cuts at: 5556 5556 Size: 5680 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4794 4978 1305 Size: 3489 184 2007 Fragments arranged by size: 3489 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3858 Fragments arranged by size: 3858 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3638 3997 4156 518 Size: 648 2472 359 159 2042 Fragments arranged by size: 2472 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2249 2359 3096 3773 4921 5166 1606 Size: 643 110 737 677 1148 245 2120 Fragments arranged by size: 2120 1148 737 677 643 245 110 NcoI C'CATG_G Cuts at: 513 1065 2004 2840 513 Size: 552 939 836 3353 Fragments arranged by size: 3353 939 836 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5680 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5680 NotI GC'GGCC_GC Cuts at: 3127 3127 Size: 5680 NruI TCG'CGA Cuts at: 2334 2334 Size: 5680 NspI r_CATG'y Cuts at: 1132 1229 3038 4583 1132 Size: 97 1809 1545 2229 Fragments arranged by size: 2229 1809 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5680 PciI A'CATG_T Cuts at: 4579 4579 Size: 5680 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5680 PfoI T'CCnGG_A Cuts at: 2154 2154 Size: 5680 PmeI GTTT'AAAC Cuts at: 2984 2984 Size: 5680 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3749 3781 5262 5294 3749 Size: 32 1481 32 4135 Fragments arranged by size: 4135 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5680 PsiI TTA'TAA Cuts at: 3250 3250 Size: 5680 PstI C_TGCA'G Cuts at: 830 2358 3032 830 Size: 1528 674 3478 Fragments arranged by size: 3478 1528 674 PvuI CG_AT'CG Cuts at: 2001 2651 2001 Size: 650 5030 SacI G_AGCT'C Cuts at: 2154 2154 Size: 5680 SacII CC_GC'GG Cuts at: 2250 2250 Size: 5680 SalI G'TCGA_C Cuts at: 1936 3022 1936 Size: 1086 4594 SapI GCTCTTCn'nnn_ Cuts at: 2505 2505 Size: 5680 SbfI CC_TGCA'GG Cuts at: 3032 3032 Size: 5680 ScaI AGT'ACT Cuts at: 3928 5640 3928 Size: 1712 3968 SfcI C'TryA_G Cuts at: 826 1045 2354 2687 3028 4163 4844 5035 826 Size: 219 1309 333 341 1135 681 191 1471 Fragments arranged by size: 1471 1309 1135 681 341 333 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5680 SmaI CCC'GGG Cuts at: 1872 2345 3007 1872 Size: 473 662 4545 Fragments arranged by size: 4545 662 473 SmlI C'TyrA_G Cuts at: 820 1017 1943 2556 3784 4685 4947 820 Size: 197 926 613 1228 901 262 1553 Fragments arranged by size: 1553 1228 926 901 613 262 197 SnaBI TAC'GTA Cuts at: 493 3471 493 Size: 2978 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5680 SphI G_CATG'C Cuts at: 1132 3038 1132 Size: 1906 3774 SspI AAT'ATT Cuts at: 5 52 3604 5 Size: 47 3552 2081 Fragments arranged by size: 3552 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5680 StyI C'CwwG_G Cuts at: 513 1065 2004 2840 3119 513 Size: 552 939 836 279 3074 Fragments arranged by size: 3074 939 836 552 279 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2901 3707 3724 3877 4062 4507 325 Size: 1255 1321 806 17 153 185 445 1498 Fragments arranged by size: 1498 1321 1255 806 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2017 2932 3926 Size: 274 80 33 51 1443 40 915 994 Cuts at: 3926 5638 96 Size: 1712 138 Fragments arranged by size: 1712 1443 994 915 274 138 80 51 40 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5640 5672 5640 Size: 32 5648 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5680 XbaI T'CTAG_A Cuts at: 2030 3016 2030 Size: 986 4694 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2898 2898 Size: 5680 XhoI C'TCGA_G Cuts at: 1943 2556 1943 Size: 613 5067 XmaI C'CCGG_G Cuts at: 1870 2343 3005 1870 Size: 473 662 4545 Fragments arranged by size: 4545 662 473 XmnI GAAnn'nnTTC Cuts at: 3809 3809 Size: 5680 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3488 276 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI SexAI SfiI SfoI SrfI SwaI