(Linear) (Six-base) MAPSORT of: KIEE5353.seq Check: 2153 from: 1 to: 975 KIEE5353.orf With 182 enzymes: SgfI * January 29, 2014 11:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 597 833 975 Size: 597 236 142 Fragments arranged by size: 597 236 142 Acc65I G'GTAC_C Cuts at: 0 840 975 Size: 840 135 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 244 481 619 975 Size: 244 237 138 356 Fragments arranged by size: 356 244 237 138 AlwNI CAG_nnn'CTG Cuts at: 0 853 975 Size: 853 122 ApoI r'AATT_y Cuts at: 0 99 559 726 975 Size: 99 460 167 249 Fragments arranged by size: 460 249 167 99 AseI AT'TA_AT Cuts at: 0 692 975 Size: 692 283 AvaI C'yCGr_G Cuts at: 0 338 551 975 Size: 338 213 424 Fragments arranged by size: 424 338 213 BamHI G'GATC_C Cuts at: 0 273 975 Size: 273 702 BanI G'GyrC_C Cuts at: 0 840 975 Size: 840 135 BanII G_rGCy'C Cuts at: 0 149 975 Size: 149 826 BbsI GAAGACnn'nnnn_ Cuts at: 0 232 632 975 Size: 232 400 343 Fragments arranged by size: 400 343 232 BbvCI CC'TCA_GC Cuts at: 0 256 975 Size: 256 719 Bme1580I G_kGCm'C Cuts at: 0 22 975 Size: 22 953 BmrI ACTGGGnnnn_n' Cuts at: 0 705 975 Size: 705 270 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 566 975 Size: 566 409 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 875 975 Size: 875 100 Bpu10I CC'TnA_GC Cuts at: 0 256 975 Size: 256 719 BsaBI GATnn'nnATC Cuts at: 0 278 975 Size: 278 697 BsaHI Gr'CG_yC Cuts at: 0 239 975 Size: 239 736 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 373 586 691 975 Size: 373 213 105 284 Fragments arranged by size: 373 284 213 105 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 883 975 Size: 883 92 BsiEI CG_ry'CG Cuts at: 0 646 975 Size: 646 329 BsiHKAI G_wGCw'C Cuts at: 0 149 881 975 Size: 149 732 94 Fragments arranged by size: 732 149 94 Bsp1286I G_dGCh'C Cuts at: 0 22 149 881 975 Size: 22 127 732 94 Fragments arranged by size: 732 127 94 22 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 597 833 975 Size: 597 236 142 Fragments arranged by size: 597 236 142 BsrDI GCAATG_nn' Cuts at: 0 172 975 Size: 172 803 BsrFI r'CCGG_y Cuts at: 0 344 975 Size: 344 631 BsrGI T'GTAC_A Cuts at: 0 12 927 975 Size: 12 915 48 Fragments arranged by size: 915 48 12 BstAPI GCAn_nnn'nTGC Cuts at: 0 609 975 Size: 609 366 BstYI r'GATC_y Cuts at: 0 273 831 975 Size: 273 558 144 Fragments arranged by size: 558 273 144 BtgI C'CryG_G Cuts at: 0 242 835 975 Size: 242 593 140 Fragments arranged by size: 593 242 140 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 310 975 Size: 310 665 DraI TTT'AAA Cuts at: 0 422 975 Size: 422 553 EarI CTCTTCn'nnn_ Cuts at: 0 401 500 975 Size: 401 99 475 Fragments arranged by size: 475 401 99 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 908 975 Size: 908 67 EcoICRI GAG'CTC Cuts at: 0 147 975 Size: 147 828 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 244 481 619 875 975 Size: 244 237 138 256 100 Fragments arranged by size: 256 244 237 138 100 EcoNI CCTnn'n_nnAGG Cuts at: 0 487 975 Size: 487 488 EcoO109I rG'GnC_Cy Cuts at: 0 850 975 Size: 850 125 EcoRI G'AATT_C Cuts at: 0 99 559 726 975 Size: 99 460 167 249 Fragments arranged by size: 460 249 167 99 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 87 119 648 680 975 Size: 87 32 529 32 295 Fragments arranged by size: 529 295 87 32 32 HincII GTy'rAC Cuts at: 0 159 975 Size: 159 816 HindIII A'AGCT_T Cuts at: 0 740 975 Size: 740 235 HpaI GTT'AAC Cuts at: 0 159 975 Size: 159 816 KpnI G_GTAC'C Cuts at: 0 844 975 Size: 844 131 MspA1I CmG'CkG Cuts at: 0 244 354 975 Size: 244 110 621 Fragments arranged by size: 621 244 110 NcoI C'CATG_G Cuts at: 0 835 975 Size: 835 140 NruI TCG'CGA Cuts at: 0 329 975 Size: 329 646 PfoI T'CCnGG_A Cuts at: 0 149 975 Size: 149 826 PstI C_TGCA'G Cuts at: 0 353 975 Size: 353 622 PvuI CG_AT'CG Cuts at: 0 646 975 Size: 646 329 SacI G_AGCT'C Cuts at: 0 149 975 Size: 149 826 SacII CC_GC'GG Cuts at: 0 245 975 Size: 245 730 SapI GCTCTTCn'nnn_ Cuts at: 0 500 975 Size: 500 475 SfcI C'TryA_G Cuts at: 0 349 682 975 Size: 349 333 293 Fragments arranged by size: 349 333 293 SmaI CCC'GGG Cuts at: 0 340 975 Size: 340 635 SmlI C'TyrA_G Cuts at: 0 551 975 Size: 551 424 StyI C'CwwG_G Cuts at: 0 835 975 Size: 835 140 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 896 975 Size: 896 79 TatI w'GTAC_w Cuts at: 0 12 927 975 Size: 12 915 48 Fragments arranged by size: 915 48 12 XbaI T'CTAG_A Cuts at: 0 25 975 Size: 25 950 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 893 975 Size: 893 82 XhoI C'TCGA_G Cuts at: 0 551 975 Size: 551 424 XmaI C'CCGG_G Cuts at: 0 338 975 Size: 338 637 Enzymes that do cut: AarI Acc65I AcuI AlwNI ApoI AseI AvaI BamHI BanI BanII BbsI BbvCI Bme1580I BmrI BplI BpmI Bpu10I BsaBI BsaHI BseRI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstYI BtgI BtgZI DraI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII HindIII HpaI KpnI MspA1I NcoI NruI PfoI PstI PvuI SacI SacII SapI SfcI SmaI SmlI StyI TaqII TatI XbaI XcmI XhoI XmaI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BpuEI BsaI BsaAI BsaWI BsaXI BseYI BsiWI BsmI BsmBI BspEI BspHI BsrBI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraIII DrdI EaeI EagI EcoRV FalI FseI FspI FspAI HaeII KasI MfeI MluI MmeI MscI MslI NaeI NarI NdeI NgoMIV NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuII RsrII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XmnI ZraI