################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BciVI BclI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmFI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrFI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XcmI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### ApaI 297 GGGCCC GGGCC^C Asp700I 613 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 211 GTGCTC G[AT]GC[AT]^C AvaI 377 CCCGGG C^[CT]CG[GA]G BanII 297 GGGCCC G[GA]GC[CT]^C BbvCI 54 GCTGAGG CCTCAGC(-5/-2) BglII 723 AGATCT A^GATCT BseRI 608 GAGGAG GAGGAG(10/8) BsgI 206 GTGCAG GTGCAG(16/14) BsiHKAI 211 GTGCTC G[AT]GC[AT]^C BsmBI 68 CGTCTC CGTCTC(1/5) BsoBI 377 CCCGGG C^[CT]CG[GA]G Bsp1407I 731 TGTACA T^GTACA BspMI 807 GCAGGT ACCTGC(4/8) BsrDI 517 GCAATG GCAATG(2/0) BsrGI 731 TGTACA T^GTACA BssHII 28 GCGCGC G^CGCGC BssSI 773 CACGAG CACGAG(-5/-1) BstXI 335 CCAGTGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 723 AGATCT [GA]^GATC[CT] DraI 901 TTTAAA TTT^AAA EcoNI 571 CCTCAAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 82 GATATC GAT^ATC HgaI 67 GCGTC GACGC(5/10) HincII 322 GTCAAC GT[CT]^[GA]AC HindII 322 GTCAAC GT[CT]^[GA]AC MaeII 9 ACGT A^CGT MflI 723 AGATCT [GA]^GATC[CT] MspA1I 77 CAGCTG C[AC]G^C[GT]G NcoI 788 CCATGG C^CATGG NspI 959 ACATGC [GA]CATG^[CT] PleI 320 GAGTC GAGTC(4/5) PpuMI 380 GGGTCCT [GA]G^G[AT]CC[CT] PvuII 77 CAGCTG CAG^CTG SmaI 377 CCCGGG CCC^GGG SmlI 572 CTCAAG C^T[CT][GA]AG SspBI 731 TGTACA T^GTACA Tsp45I 771 GTCAC ^GT[GC]AC XbaI 588 TCTAGA T^CTAGA XhoII 723 AGATCT [GA]^GATC[CT] XmaCI 377 CCCGGG C^CCGGG XmaI 377 CCCGGG C^CCGGG XmnI 613 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccII 29 CGCG CG^CG 31 CGCG AcsI 448 AAATTT [GA]^AATT[CT] 492 GAATTT ApoI 448 AAATTT [GA]^AATT[CT] 492 GAATTT AvaII 381 GGTCC G^G[AT]CC 837 GGTCC BmyI 211 GTGCTC G[AGT]GC[ACT]^C 297 GGGCCC Bsp1286I 211 GTGCTC G[AGT]GC[ACT]^C 297 GGGCCC BstUI 29 CGCG CG^CG 31 CGCG DsaI 788 CCATGG C^C[GA][CT]GG 833 CCGTGG EcoT14I 788 CCATGG C^C[AT][AT]GG 942 CCAAGG HaeII 3 GGCGCT [GA]GCGC^[CT] 48 GGCGCT HphI 810 GGTGA GGTGA(8/7) 816 TCACC MaeIII 771 GTCAC ^GT[ACGT]AC 991 GTTAC MvnI 29 CGCG CG^CG 31 CGCG NlaIII 789 CATG CATG^ 960 CATG SfaNI 399 GATGC GCATC(5/9) 592 GATGC StyI 788 CCATGG C^C[AT][AT]GG 942 CCAAGG TfiI 183 GATTC G^A[AT]TC 813 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AfaI AluI BbsI BbvI BcnI BfaI BpuAI BsaI BsaJI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeI MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI TaqI Tru9I TseI Tsp509I TspRI TthHB8I