(Circular) (Six-base) MAPSORT of: pFN21AE5545.seq Check: 3892 from: 1 to: 5869 pFN21AE5545 5869 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2412 2412 Size: 5869 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3679 278 Size: 53 83 186 936 2143 2468 Fragments arranged by size: 2468 2143 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3212 1937 Size: 1275 4594 Acc65I G'GTAC_C Cuts at: 3190 3190 Size: 5869 AclI AA'CG_TT Cuts at: 1563 3996 4369 1563 Size: 2433 373 3063 Fragments arranged by size: 3063 2433 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3932 5316 5584 1632 Size: 2300 1384 268 1917 Fragments arranged by size: 2300 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5672 AflIII A'CryG_T Cuts at: 1203 2331 4768 5745 1203 Size: 1128 2437 977 1327 Fragments arranged by size: 2437 1327 1128 977 AgeI A'CCGG_T Cuts at: 4700 4700 Size: 5869 AhdI GACnn_n'nnGTC Cuts at: 4598 4598 Size: 5869 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5869 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5869 AlwNI CAG_nnn'CTG Cuts at: 2015 2602 2626 3004 3612 5184 2015 Size: 587 24 378 608 1572 2700 Fragments arranged by size: 2700 1572 608 587 378 24 ApaI G_GGCC'C Cuts at: 2063 2063 Size: 5869 ApaLI G'TGCA_C Cuts at: 3926 5082 3926 Size: 1156 4713 ApoI r'AATT_y Cuts at: 1493 3178 3404 3556 5665 1493 Size: 1685 226 152 2109 1697 Fragments arranged by size: 2109 1697 1685 226 152 AseI AT'TA_AT Cuts at: 160 1032 4423 160 Size: 872 3391 1606 Fragments arranged by size: 3391 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5869 AvaI C'yCGr_G Cuts at: 1870 1943 2049 2135 2411 2552 2910 2989 3137 Size: 73 106 86 276 141 358 79 148 Cuts at: 3137 3194 1870 Size: 57 4545 Fragments arranged by size: 4545 358 276 148 141 106 86 79 73 57 BamHI G'GATC_C Cuts at: 3199 3566 3199 Size: 367 5502 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3046 3190 4645 618 Size: 325 217 627 1259 144 1455 1842 Fragments arranged by size: 1842 1455 1259 627 325 217 144 BanII G_rGCy'C Cuts at: 1391 2063 2135 2169 2640 3144 3188 1391 Size: 672 72 34 471 504 44 4072 Fragments arranged by size: 4072 672 504 471 72 44 34 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2665 928 Size: 971 766 4132 Fragments arranged by size: 4132 971 766 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4060 4094 1903 Size: 34 2123 34 3678 Fragments arranged by size: 3678 2123 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3761 4971 3761 Size: 1210 4659 BclI T'GATC_A Cuts at: 1548 1548 Size: 5869 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2192 3110 4480 Size: 107 122 71 631 605 520 918 1370 Cuts at: 4480 136 Size: 1525 Fragments arranged by size: 1525 1370 918 631 605 520 122 107 71 BglII A'GATC_T Cuts at: 2325 5864 2325 Size: 3539 2330 BlpI GC'TnA_GC Cuts at: 2570 2786 3286 2570 Size: 216 500 5153 Fragments arranged by size: 5153 500 216 Bme1580I G_kGCm'C Cuts at: 1790 2063 2900 3051 3930 5086 1790 Size: 273 837 151 879 1156 2573 Fragments arranged by size: 2573 1156 879 837 273 151 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5869 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2238 2297 3600 Size: 416 53 83 399 24 821 59 1303 Cuts at: 3600 4558 442 Size: 958 1753 Fragments arranged by size: 1753 1303 958 821 416 399 83 59 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5869 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2197 3139 2197 Size: 942 4927 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2624 3110 4529 1239 Size: 815 570 486 1419 2579 Fragments arranged by size: 2579 1419 815 570 486 Bpu10I CC'TnA_GC Cuts at: 2762 2762 Size: 5869 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3994 4859 5157 3994 Size: 865 298 4706 Fragments arranged by size: 4706 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2570 4532 882 Size: 617 1071 1962 2219 Fragments arranged by size: 2219 1962 1071 617 BsaAI yAC'GTr Cuts at: 493 1204 2566 3660 493 Size: 711 1362 1094 2702 Fragments arranged by size: 2702 1362 1094 711 BsaBI GATnn'nnATC Cuts at: 5863 5863 Size: 5869 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2259 3676 4058 4693 Size: 53 83 186 936 726 1417 382 635 Cuts at: 4693 275 Size: 1451 Fragments arranged by size: 1451 1417 936 726 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2427 4302 4700 4974 5121 5532 2427 Size: 1875 398 274 147 411 2764 Fragments arranged by size: 2764 1875 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2408 2438 3126 3156 1178 Size: 30 165 30 1005 30 688 30 3891 Fragments arranged by size: 3891 1005 688 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2429 2432 2570 2753 2837 1186 Size: 1243 3 138 183 84 4218 Fragments arranged by size: 4218 1243 183 138 84 3 BseYI C'CCAG_C Cuts at: 2362 2589 2920 5072 5568 2362 Size: 227 331 2152 496 2663 Fragments arranged by size: 2663 2152 496 331 227 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2390 1163 Size: 573 654 4642 Fragments arranged by size: 4642 654 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3319 4080 5108 1540 Size: 275 186 1318 761 1028 2301 Fragments arranged by size: 2301 1318 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3144 3930 4015 5086 3144 Size: 786 85 1071 3927 Fragments arranged by size: 3927 1071 786 85 BsmI GAATG_Cn' Cuts at: 3380 3473 3380 Size: 93 5776 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2135 2169 2640 2900 3051 3144 Size: 399 273 72 34 471 260 151 93 Cuts at: 3144 3188 3930 4015 5086 1391 Size: 44 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 471 399 273 260 151 93 85 72 44 34 BspEI T'CCGG_A Cuts at: 5532 5532 Size: 5869 BspHI T'CATG_A Cuts at: 2319 3758 5488 2319 Size: 1439 1730 2700 Fragments arranged by size: 2700 1730 1439 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2412 3224 844 Size: 1568 812 3489 Fragments arranged by size: 3489 1568 812 BsrBI CCG'CTC Cuts at: 1394 1823 2224 2782 2917 3756 1394 Size: 429 401 558 135 839 3507 Fragments arranged by size: 3507 839 558 429 401 135 BsrDI GCAATG_nn' Cuts at: 65 1248 2376 4364 4538 65 Size: 1183 1128 1988 174 1396 Fragments arranged by size: 1988 1396 1183 1128 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4513 4700 1700 Size: 110 2703 187 2869 Fragments arranged by size: 2869 2703 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5869 BssHII G'CGCG_C Cuts at: 1925 2644 1925 Size: 719 5150 BssSI C'ACGA_G Cuts at: 2489 3070 3929 4941 2489 Size: 581 859 1012 3417 Fragments arranged by size: 3417 1012 859 581 BstAPI GCAn_nnn'nTGC Cuts at: 794 2015 2472 794 Size: 1221 457 4191 Fragments arranged by size: 4191 1221 457 BstBI TT'CG_AA Cuts at: 2703 4673 2703 Size: 1970 3899 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5869 BstXI CCAn_nnnn'nTGG Cuts at: 2389 2389 Size: 5869 BstYI r'GATC_y Cuts at: 1972 2325 3199 3566 3951 3968 5420 5506 5518 Size: 353 874 367 385 17 1452 86 12 Cuts at: 5518 5864 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 874 385 367 353 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2240 2240 Size: 5869 BtgI C'CryG_G Cuts at: 513 1065 2004 3105 513 Size: 552 939 1101 3277 Fragments arranged by size: 3277 1101 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2052 487 Size: 683 56 826 4304 Fragments arranged by size: 4304 826 683 56 BtsI GCAGTG_nn' Cuts at: 3385 4179 4199 3385 Size: 794 20 5055 Fragments arranged by size: 5055 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5869 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5834 DraI TTT'AAA Cuts at: 3173 3173 Size: 5869 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4876 809 Size: 507 3560 1802 Fragments arranged by size: 3560 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2307 3316 4205 Size: 54 1425 50 275 18 477 1009 889 Cuts at: 4205 5834 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1009 889 477 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3316 1537 Size: 275 1504 4090 Fragments arranged by size: 4090 1504 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3799 1353 Size: 2446 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4456 4830 4976 387 Size: 1064 138 2867 374 146 1280 Fragments arranged by size: 2867 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3142 3142 Size: 5869 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2624 3110 3932 4529 5316 5584 Size: 393 422 570 486 822 597 787 268 Cuts at: 5584 1239 Size: 1524 Fragments arranged by size: 1524 822 787 597 570 486 422 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2243 2891 2243 Size: 648 5221 EcoO109I rG'GnC_Cy Cuts at: 2059 2060 2140 2484 2548 2950 2059 Size: 1 80 344 64 402 4978 Fragments arranged by size: 4978 402 344 80 64 1 EcoRI G'AATT_C Cuts at: 3178 3556 3178 Size: 378 5491 FspI TGC'GCA Cuts at: 2840 4375 2840 Size: 1535 4334 HaeII r_GCGC'y Cuts at: 1678 2668 5016 1678 Size: 990 2348 2531 Fragments arranged by size: 2531 2348 990 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3131 3163 4510 4542 4584 Size: 32 135 32 1559 32 1347 32 42 Cuts at: 4584 4616 1373 Size: 32 2626 Fragments arranged by size: 2626 1559 1347 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3213 3459 669 Size: 988 281 1275 246 3079 Fragments arranged by size: 3079 1275 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5869 HpaI GTT'AAC Cuts at: 3459 3459 Size: 5869 KpnI G_GTAC'C Cuts at: 3194 3194 Size: 5869 MfeI C'AATT_G Cuts at: 3468 3468 Size: 5869 MluI A'CGCG_T Cuts at: 5745 5745 Size: 5869 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4983 5167 1305 Size: 3678 184 2007 Fragments arranged by size: 3678 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4047 Fragments arranged by size: 4047 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3083 3827 4186 4345 518 Size: 648 1917 744 359 159 2042 Fragments arranged by size: 2042 1917 744 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2015 2127 2254 2623 2830 3107 3285 3962 Size: 409 112 127 369 207 277 178 677 Cuts at: 3962 5110 5355 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 409 369 277 245 207 178 127 112 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4378 Fragments arranged by size: 4378 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5869 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5869 NotI GC'GGCC_GC Cuts at: 3316 3316 Size: 5869 NspI r_CATG'y Cuts at: 1132 1229 3227 4772 1132 Size: 97 1998 1545 2229 Fragments arranged by size: 2229 1998 1545 97 PasI CC'CwG_GG Cuts at: 1348 2144 1348 Size: 796 5073 PciI A'CATG_T Cuts at: 4768 4768 Size: 5869 PflMI CCAn_nnn'nTGG Cuts at: 1108 2602 1108 Size: 1494 4375 PfoI T'CCnGG_A Cuts at: 2943 2943 Size: 5869 PmeI GTTT'AAAC Cuts at: 3173 3173 Size: 5869 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3938 3970 5451 5483 3938 Size: 32 1481 32 4324 Fragments arranged by size: 4324 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2484 2548 2484 Size: 64 5805 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5869 PsiI TTA'TAA Cuts at: 3439 3439 Size: 5869 PspOMI G'GGCC_C Cuts at: 2059 2059 Size: 5869 PstI C_TGCA'G Cuts at: 830 2445 2895 2958 3221 830 Size: 1615 450 63 263 3478 Fragments arranged by size: 3478 1615 450 263 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5869 PvuII CAG'CTG Cuts at: 2623 2623 Size: 5869 SacI G_AGCT'C Cuts at: 3144 3144 Size: 5869 SacII CC_GC'GG Cuts at: 3108 3108 Size: 5869 SalI G'TCGA_C Cuts at: 1936 3211 1936 Size: 1275 4594 SanDI GG'GwC_CC Cuts at: 2484 2484 Size: 5869 SbfI CC_TGCA'GG Cuts at: 2895 3221 2895 Size: 326 5543 ScaI AGT'ACT Cuts at: 4117 5829 4117 Size: 1712 4157 SexAI A'CCwGG_T Cuts at: 2088 2088 Size: 5869 SfcI C'TryA_G Cuts at: 826 1045 2441 2891 2954 3217 4352 5033 5224 Size: 219 1396 450 63 263 1135 681 191 Cuts at: 5224 826 Size: 1471 Fragments arranged by size: 1471 1396 1135 681 450 263 219 191 63 SfiI GGCCn_nnn'nGGCC Cuts at: 3110 3110 Size: 5869 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5869 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5869 SmaI CCC'GGG Cuts at: 1872 2051 3196 1872 Size: 179 1145 4545 Fragments arranged by size: 4545 1145 179 SmlI C'TyrA_G Cuts at: 820 1017 1943 3973 4874 5136 820 Size: 197 926 2030 901 262 1553 Fragments arranged by size: 2030 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3660 493 Size: 3167 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5869 SphI G_CATG'C Cuts at: 1132 3227 1132 Size: 2095 3774 SspI AAT'ATT Cuts at: 5 52 3793 5 Size: 47 3741 2081 Fragments arranged by size: 3741 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5869 StyI C'CwwG_G Cuts at: 513 1065 2004 2583 2802 3308 513 Size: 552 939 579 219 506 3074 Fragments arranged by size: 3074 939 579 552 506 219 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2354 3896 3913 4066 4251 4696 325 Size: 1255 774 1542 17 153 185 445 1498 Fragments arranged by size: 1542 1498 1255 774 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2208 2844 4115 Size: 274 80 33 51 1443 231 636 1271 Cuts at: 4115 5827 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1271 636 274 231 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5829 5861 5829 Size: 32 5837 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5869 XbaI T'CTAG_A Cuts at: 3205 3205 Size: 5869 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2302 2302 Size: 5869 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5869 XmaI C'CCGG_G Cuts at: 1870 2049 3194 1870 Size: 179 1145 4545 Fragments arranged by size: 4545 1145 179 XmnI GAAnn'nnTTC Cuts at: 3998 3998 Size: 5869 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3677 276 Size: 53 83 186 936 2143 2468 Fragments arranged by size: 2468 2143 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SapI SfoI SrfI SwaI