################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BanI BbeI BbrPI BbvCI BcgI BclI BfaI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoRV FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcyI 706 GGCGTC G[GA]^CG[CT]C ApaI 422 GGGCCC GGGCC^C AvaI 1114 CTCGAG C^[CT]CG[GA]G BalI 872 TGGCCA TGG^CCA BamHI 726 GGATCC G^GATCC BbsI 709 GTCTTC GAAGAC(2/6) BcnI 288 CCCGG CC^[GC]GG BglII 155 AGATCT A^GATCT BpuAI 709 GTCTTC GAAGAC(2/6) BsaHI 706 GGCGTC G[GA]^CG[CT]C BsaI 733 GAGACC GGTCTC(1/5) BsmFI 128 GTCCC GGGAC(10/14) BsmI 532 GCATTC GAATGC(1/-1) BsoBI 1114 CTCGAG C^[CT]CG[GA]G BsrDI 580 GCAATG GCAATG(2/0) BsrI 374 ACTGG ACTGG(1/-1) BstEII 810 GGTCACC G^GT[ACGT]ACC BstF5I 75 CATCC GGATG(2/0) BstPI 810 GGTCACC G^GT[ACGT]ACC CfoI 397 GCGC GCG^C EarI 699 GAAGAG CTCTTC(1/4) EcoO65I 810 GGTCACC G^GT[ACGT]ACC EcoRI 313 GAATTC G^AATTC EcoT22I 530 ATGCAT ATGCA^T FokI 75 CATCC GGATG(9/13) HaeII 396 GGCGCT [GA]GCGC^[CT] HapII 289 CCGG C^CGG HhaI 397 GCGC GCG^C Hin1I 706 GGCGTC G[GA]^CG[CT]C HinP1I 397 GCGC G^CGC HpaII 289 CCGG C^CGG Ksp632I 699 GAAGAG CTCTTC(1/4) MaeII 887 ACGT A^CGT MluNI 872 TGGCCA TGG^CCA MscI 872 TGGCCA TGG^CCA MspA1I 232 CAGCTG C[AC]G^C[GT]G MspI 289 CCGG C^CGG NciI 288 CCCGG CC^[GC]GG NcoI 662 CCATGG C^CATGG NsiI 530 ATGCAT ATGCA^T PaeR7I 1114 CTCGAG C^TCGAG PpuMI 126 GGGTCCC [GA]G^G[AT]CC[CT] PvuII 232 CAGCTG CAG^CTG SspI 1010 AATATT AAT^ATT XhoI 1114 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AhdI 735 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 781 GACATACTGTC AlwI 727 GATCC GGATC(4/5) 726 GGATC AspEI 735 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 781 GACATACTGTC AspHI 187 GAGCAC G[AT]GC[AT]^C 325 GAGCAC AvaII 127 GGTCC G^G[AT]CC 278 GGACC BsgI 538 CTGCAC GTGCAG(16/14) 848 GTGCAG BsiHKAI 187 GAGCAC G[AT]GC[AT]^C 325 GAGCAC Bsp1407I 479 TGTACA T^GTACA 1031 TGTACA BsrGI 479 TGTACA T^GTACA 1031 TGTACA BstYI 155 AGATCT [GA]^GATC[CT] 726 GGATCC DraI 610 TTTAAA TTT^AAA 1006 TTTAAA DsaI 662 CCATGG C^C[GA][CT]GG 980 CCGTGG Eam1105I 735 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 781 GACATACTGTC FauI 359 CCCGC CCCGC(4/6) 911 CCCGC HgaI 148 GACGC GACGC(5/10) 707 GCGTC MflI 155 AGATCT [GA]^GATC[CT] 726 GGATCC MseI 611 TTAA T^TAA 1007 TTAA MslI 328 CACCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 739 CATTGTCATG PleI 411 GAGTC GAGTC(4/5) 963 GAGTC PstI 402 CTGCAG CTGCA^G 1102 CTGCAG SfcI 402 CTGCAG C^T[GA][CT]AG 1102 CTGCAG SmlI 132 CTCAAG C^T[CT][GA]AG 1114 CTCGAG SspBI 479 TGTACA T^GTACA 1031 TGTACA Tru9I 611 TTAA T^TAA 1007 TTAA TspRI 23 CAGTG CA[GC]TG[ACGT][ACGT]^ 483 CAGTG XcmI 496 CCATTCATGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1048 CCATTCAAGCCTTGG XhoII 155 AGATCT [GA]^GATC[CT] 726 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BanII BbvI BciVI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BssKI BstNI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI HaeIII HinfI HphI ItaI MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SexAI StyI TaqI TfiI TseI Tsp45I Tsp509I TthHB8I