Restriction map for
pFN21AE5539
(Circular) (Six-base) MAPSORT of: pFN21AE5539.seq Check: 9676 from: 1 to: 5824
pFN21AE5539 5824 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3634 278
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3167 1937
Size: 1230 4594
Acc65I G'GTAC_C
Cuts at: 3145 3145
Size: 5824
AclI AA'CG_TT
Cuts at: 1563 3951 4324 1563
Size: 2388 373 3063
Fragments arranged by size:
3063 2388 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2002 3887 5271 5539 1632
Size: 370 1885 1384 268 1917
Fragments arranged by size:
1917 1885 1384 370 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5627
AflIII A'CryG_T
Cuts at: 1203 4723 5700 1203
Size: 3520 977 1327
Fragments arranged by size:
3520 1327 977
AgeI A'CCGG_T
Cuts at: 4655 4655
Size: 5824
AhdI GACnn_n'nnGTC
Cuts at: 2745 2791 4553 2745
Size: 46 1762 4016
Fragments arranged by size:
4016 1762 46
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5824
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2224 2255 2776 1184
Size: 1040 31 521 4232
Fragments arranged by size:
4232 1040 521 31
AlwNI CAG_nnn'CTG
Cuts at: 3567 5139 3567
Size: 1572 4252
ApaI G_GGCC'C
Cuts at: 2431 2431
Size: 5824
ApaLI G'TGCA_C
Cuts at: 3881 5037 3881
Size: 1156 4668
ApoI r'AATT_y
Cuts at: 1493 2318 2456 2519 3133 3359 3511 5620 1493
Size: 825 138 63 614 226 152 2109 1697
Fragments arranged by size:
2109 1697 825 614 226 152 138 63
AseI AT'TA_AT
Cuts at: 160 1032 4378 160
Size: 872 3346 1606
Fragments arranged by size:
3346 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
AvaI C'yCGr_G
Cuts at: 1870 1943 3119 3149 1870
Size: 73 1176 30 4545
Fragments arranged by size:
4545 1176 73 30
BamHI G'GATC_C
Cuts at: 2731 3154 3521 2731
Size: 423 367 5034
Fragments arranged by size:
5034 423 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3145 4600 618
Size: 325 217 627 1358 1455 1842
Fragments arranged by size:
1842 1455 1358 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2431 2840 2958 3143 1391
Size: 1040 409 118 185 4072
Fragments arranged by size:
4072 1040 409 185 118
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2707 928
Size: 971 808 4045
Fragments arranged by size:
4045 971 808
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4015 4049 1903
Size: 34 2078 34 3678
Fragments arranged by size:
3678 2078 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2287 2601 2929 3716 4926 2287
Size: 314 328 787 1210 3185
Fragments arranged by size:
3185 1210 787 328 314
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5824
BfrBI ATG'CAT
Cuts at: 2537 2537
Size: 5824
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4435 136
Size: 107 122 71 631 605 2763 1525
Fragments arranged by size:
2763 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2160 5819 2160
Size: 3659 2165
BlpI GC'TnA_GC
Cuts at: 3241 3241
Size: 5824
Bme1580I G_kGCm'C
Cuts at: 1790 2431 3885 5041 1790
Size: 641 1454 1156 2573
Fragments arranged by size:
2573 1454 1156 641
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5824
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2389 3555 4513
Size: 416 53 83 399 24 972 1166 958
Cuts at: 4513 442
Size: 1753
Fragments arranged by size:
1753 1166 972 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4484 1239
Size: 3245 2579
Bpu10I CC'TnA_GC
Cuts at: 2190 2190
Size: 5824
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2122 3949 4814 5112 2122
Size: 1827 865 298 2834
Fragments arranged by size:
2834 1827 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2732 4487 882
Size: 617 1233 1755 2219
Fragments arranged by size:
2219 1755 1233 617
BsaAI yAC'GTr
Cuts at: 493 1204 3615 493
Size: 711 2411 2702
Fragments arranged by size:
2702 2411 711
BsaBI GATnn'nnATC
Cuts at: 5818 5818
Size: 5824
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2712 3631 4013 4648
Size: 53 83 186 936 1179 919 382 635
Cuts at: 4648 275
Size: 1451
Fragments arranged by size:
1451 1179 936 919 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4257 4655 4929 5076 5487 4257
Size: 398 274 147 411 4594
Fragments arranged by size:
4594 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2024 2039 2054 2069 2573
Size: 30 165 30 621 15 15 15 504
Cuts at: 2573 2603 1178
Size: 30 4399
Fragments arranged by size:
4399 621 504 165 30 30 30 15
15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5824
BseYI C'CCAG_C
Cuts at: 2184 2469 5027 5523 2184
Size: 285 2558 496 2485
Fragments arranged by size:
2558 2485 496 285
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2528 2874 1163
Size: 573 792 346 4113
Fragments arranged by size:
4113 792 573 346
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3274 4035 5063 1540
Size: 275 186 1273 761 1028 2301
Fragments arranged by size:
2301 1273 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2196 2334 3885 3970 5041 2196
Size: 138 1551 85 1071 2979
Fragments arranged by size:
2979 1551 1071 138 85
BsmI GAATG_Cn'
Cuts at: 2537 3335 3428 2537
Size: 798 93 4933
Fragments arranged by size:
4933 798 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2334 2431 2840 2958 3143 3885
Size: 399 406 138 97 409 118 185 742
Cuts at: 3885 3970 5041 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 409 406 399 185 138
118 97 85
BspEI T'CCGG_A
Cuts at: 5487 5487
Size: 5824
BspHI T'CATG_A
Cuts at: 3713 5443 3713
Size: 1730 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3179 844
Size: 2335 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3711 1394
Size: 429 1888 3507
Fragments arranged by size:
3507 1888 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2592 4319 4493 65
Size: 1183 1344 1727 174 1396
Fragments arranged by size:
1727 1396 1344 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4468 4655 1700
Size: 110 2658 187 2869
Fragments arranged by size:
2869 2658 187 110
BsrGI T'GTAC_A
Cuts at: 96 2484 3036 96
Size: 2388 552 2884
Fragments arranged by size:
2884 2388 552
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5824
BssSI C'ACGA_G
Cuts at: 3884 4896 3884
Size: 1012 4812
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5824
BstBI TT'CG_AA
Cuts at: 4628 4628
Size: 5824
BstEII G'GTnAC_C
Cuts at: 1184 2815 1184
Size: 1631 4193
BstYI r'GATC_y
Cuts at: 1972 2160 2731 3154 3521 3906 3923 5375 5461
Size: 188 571 423 367 385 17 1452 86
Cuts at: 5461 5473 5819 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 571 423 385 367 346 188
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2667 2985 513
Size: 552 1602 318 3352
Fragments arranged by size:
3352 1602 552 318
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5085
Fragments arranged by size:
5085 683 56
BtsI GCAGTG_nn'
Cuts at: 3340 4134 4154 3340
Size: 794 20 5010
Fragments arranged by size:
5010 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5824
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5789
DraI TTT'AAA
Cuts at: 2617 3013 3128 2617
Size: 396 115 5313
Fragments arranged by size:
5313 396 115
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4831 809
Size: 507 3515 1802
Fragments arranged by size:
3515 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2313 2865 2877
Size: 54 1425 50 275 18 483 552 12
Cuts at: 2877 3271 4160 5789 8
Size: 394 889 1629 43
Fragments arranged by size:
1629 1425 889 552 483 394 275 54
50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 3271 1537
Size: 275 1459 4090
Fragments arranged by size:
4090 1459 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2699 3754 1353
Size: 1346 1055 3423
Fragments arranged by size:
3423 1346 1055
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2347 4411 4785 4931 387
Size: 1064 138 758 2064 374 146 1280
Fragments arranged by size:
2064 1280 1064 758 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2002 3887 4484 5271 5539 1239
Size: 393 370 1885 597 787 268 1524
Fragments arranged by size:
1885 1524 787 597 393 370 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2704 2969 3066 2704
Size: 265 97 5462
Fragments arranged by size:
5462 265 97
EcoO109I rG'GnC_Cy
Cuts at: 2132 2142 2427 2979 2132
Size: 10 285 552 4977
Fragments arranged by size:
4977 552 285 10
EcoRI G'AATT_C
Cuts at: 2318 3133 3511 2318
Size: 815 378 4631
Fragments arranged by size:
4631 815 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 5824
FspI TGC'GCA
Cuts at: 4330 4330
Size: 5824
HaeII r_GCGC'y
Cuts at: 1678 2405 4971 1678
Size: 727 2566 2531
Fragments arranged by size:
2566 2531 727
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 2731 2763 2777
Size: 32 135 32 467 32 660 32 14
Cuts at: 2777 2809 4465 4497 4539 4571 1373
Size: 32 1656 32 42 32 2626
Fragments arranged by size:
2626 1656 660 467 135 42 32 32
32 32 32 32 32 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3168 3414 669
Size: 988 281 1230 246 3079
Fragments arranged by size:
3079 1230 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5824
HpaI GTT'AAC
Cuts at: 3414 3414
Size: 5824
KpnI G_GTAC'C
Cuts at: 3149 3149
Size: 5824
MfeI C'AATT_G
Cuts at: 3423 3423
Size: 5824
MluI A'CGCG_T
Cuts at: 5700 5700
Size: 5824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2072 2621 4938 5122 1305
Size: 767 549 2317 184 2007
Fragments arranged by size:
2317 2007 767 549 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2879 10
Size: 54 1425 343 1047 2955
Fragments arranged by size:
2955 1425 1047 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2337 2748 3782 4141 4300 518
Size: 648 1171 411 1034 359 159 2042
Fragments arranged by size:
2042 1171 1034 648 411 359 159
MspA1I CmG'CkG
Cuts at: 1606 2239 3240 3917 5065 5310 1606
Size: 633 1001 677 1148 245 2120
Fragments arranged by size:
2120 1148 1001 677 633 245
NcoI C'CATG_G
Cuts at: 513 1065 2667 513
Size: 552 1602 3670
Fragments arranged by size:
3670 1602 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5824
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5824
NotI GC'GGCC_GC
Cuts at: 3271 3271
Size: 5824
NsiI A_TGCA'T
Cuts at: 2539 2539
Size: 5824
NspI r_CATG'y
Cuts at: 1132 1229 3182 4727 1132
Size: 97 1953 1545 2229
Fragments arranged by size:
2229 1953 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5824
PciI A'CATG_T
Cuts at: 4723 4723
Size: 5824
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5824
PfoI T'CCnGG_A
Cuts at: 2279 2699 3093 2279
Size: 420 394 5010
Fragments arranged by size:
5010 420 394
PmeI GTTT'AAAC
Cuts at: 3128 3128
Size: 5824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3893 3925 5406 5438 3893
Size: 32 1481 32 4279
Fragments arranged by size:
4279 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2132 2132
Size: 5824
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5824
PsiI TTA'TAA
Cuts at: 2070 3394 2070
Size: 1324 4500
PspOMI G'GGCC_C
Cuts at: 2427 2427
Size: 5824
PstI C_TGCA'G
Cuts at: 830 2411 3111 3176 830
Size: 1581 700 65 3478
Fragments arranged by size:
3478 1581 700 65
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5824
PvuII CAG'CTG
Cuts at: 2239 2239
Size: 5824
SalI G'TCGA_C
Cuts at: 1936 3166 1936
Size: 1230 4594
SanDI GG'GwC_CC
Cuts at: 2132 2132
Size: 5824
SbfI CC_TGCA'GG
Cuts at: 3176 3176
Size: 5824
ScaI AGT'ACT
Cuts at: 4072 5784 4072
Size: 1712 4112
SexAI A'CCwGG_T
Cuts at: 2267 2724 2819 2267
Size: 457 95 5272
Fragments arranged by size:
5272 457 95
SfcI C'TryA_G
Cuts at: 826 1045 2407 3107 3172 4307 4988 5179 826
Size: 219 1362 700 65 1135 681 191 1471
Fragments arranged by size:
1471 1362 1135 700 681 219 191 65
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5824
SmaI CCC'GGG
Cuts at: 1872 3151 1872
Size: 1279 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2137 3119 3928 4829 5091 820
Size: 197 926 194 982 809 901 262 1553
Fragments arranged by size:
1553 982 926 901 809 262 197 194
SnaBI TAC'GTA
Cuts at: 493 3615 493
Size: 3122 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5824
SphI G_CATG'C
Cuts at: 1132 3182 1132
Size: 2050 3774
SspI AAT'ATT
Cuts at: 5 52 3017 3748 5
Size: 47 2965 731 2081
Fragments arranged by size:
2965 2081 731 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5824
StyI C'CwwG_G
Cuts at: 513 1065 2510 2667 3062 3263 513
Size: 552 1445 157 395 201 3074
Fragments arranged by size:
3074 1445 552 395 201 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3851 3868 4021 4206 4651 325
Size: 1255 2271 17 153 185 445 1498
Fragments arranged by size:
2271 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2484 3036 4070
Size: 274 80 33 51 1443 507 552 1034
Cuts at: 4070 5782 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1034 552 507 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5784 5816 5784
Size: 32 5792
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5824
XbaI T'CTAG_A
Cuts at: 3160 3160
Size: 5824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2508 3060 2508
Size: 552 5272
XhoI C'TCGA_G
Cuts at: 1943 3119 1943
Size: 1176 4648
XmaI C'CCGG_G
Cuts at: 1870 3149 1870
Size: 1279 4545
XmnI GAAnn'nnTTC
Cuts at: 3953 3953
Size: 5824
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3632 276
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspXI PsrI RsrII SacI SacII SapI
SfiI SfoI SrfI SwaI