(Linear) (Six-base) MAPSORT of: KIEE5539.seq Check: 1517 from: 1 to: 1119 KIEE5539.orf With 182 enzymes: SgfI * January 29, 2014 12:04 .. AhdI GACnn_n'nnGTC Cuts at: 0 740 786 1119 Size: 740 46 333 Fragments arranged by size: 740 333 46 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 219 250 771 1119 Size: 219 31 521 348 Fragments arranged by size: 521 348 219 31 ApaI G_GGCC'C Cuts at: 0 426 1119 Size: 426 693 ApoI r'AATT_y Cuts at: 0 313 451 514 1119 Size: 313 138 63 605 Fragments arranged by size: 605 313 138 63 AvaI C'yCGr_G Cuts at: 0 1114 1119 Size: 1114 5 BamHI G'GATC_C Cuts at: 0 726 1119 Size: 726 393 BanII G_rGCy'C Cuts at: 0 426 835 953 1119 Size: 426 409 118 166 Fragments arranged by size: 426 409 166 118 BbsI GAAGACnn'nnnn_ Cuts at: 0 702 1119 Size: 702 417 BciVI GTATCCnnnnn_n' Cuts at: 0 282 596 924 1119 Size: 282 314 328 195 Fragments arranged by size: 328 314 282 195 BfrBI ATG'CAT Cuts at: 0 532 1119 Size: 532 587 BglII A'GATC_T Cuts at: 0 155 1119 Size: 155 964 Bme1580I G_kGCm'C Cuts at: 0 426 1119 Size: 426 693 BmrI ACTGGGnnnn_n' Cuts at: 0 384 1119 Size: 384 735 Bpu10I CC'TnA_GC Cuts at: 0 185 1119 Size: 185 934 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 117 1119 Size: 117 1002 BsaI GGTCTCn'nnnn_ Cuts at: 0 727 1119 Size: 727 392 BsaHI Gr'CG_yC Cuts at: 0 707 1119 Size: 707 412 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 19 34 49 64 568 598 1119 Size: 19 15 15 15 504 30 521 Fragments arranged by size: 521 504 30 19 15 15 15 BseYI C'CCAG_C Cuts at: 0 179 464 1119 Size: 179 285 655 Fragments arranged by size: 655 285 179 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 523 869 1119 Size: 523 346 250 Fragments arranged by size: 523 346 250 BsiHKAI G_wGCw'C Cuts at: 0 191 329 1119 Size: 191 138 790 Fragments arranged by size: 790 191 138 BsmI GAATG_Cn' Cuts at: 0 532 1119 Size: 532 587 Bsp1286I G_dGCh'C Cuts at: 0 191 329 426 835 953 1119 Size: 191 138 97 409 118 166 Fragments arranged by size: 409 191 166 138 118 97 BsrDI GCAATG_nn' Cuts at: 0 587 1119 Size: 587 532 BsrGI T'GTAC_A Cuts at: 0 479 1031 1119 Size: 479 552 88 Fragments arranged by size: 552 479 88 BstEII G'GTnAC_C Cuts at: 0 810 1119 Size: 810 309 BstYI r'GATC_y Cuts at: 0 155 726 1119 Size: 155 571 393 Fragments arranged by size: 571 393 155 BtgI C'CryG_G Cuts at: 0 662 980 1119 Size: 662 318 139 Fragments arranged by size: 662 318 139 DraI TTT'AAA Cuts at: 0 612 1008 1119 Size: 612 396 111 Fragments arranged by size: 612 396 111 EaeI y'GGCC_r Cuts at: 0 308 860 872 1119 Size: 308 552 12 247 Fragments arranged by size: 552 308 247 12 EarI CTCTTCn'nnn_ Cuts at: 0 694 1119 Size: 694 425 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 342 1119 Size: 342 777 EcoNI CCTnn'n_nnAGG Cuts at: 0 699 964 1061 1119 Size: 699 265 97 58 Fragments arranged by size: 699 265 97 58 EcoO109I rG'GnC_Cy Cuts at: 0 127 137 422 974 1119 Size: 127 10 285 552 145 Fragments arranged by size: 552 285 145 127 10 EcoRI G'AATT_C Cuts at: 0 313 1119 Size: 313 806 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 27 1119 Size: 27 1092 HaeII r_GCGC'y Cuts at: 0 400 1119 Size: 400 719 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 34 66 726 758 772 804 1119 Size: 34 32 660 32 14 32 315 Fragments arranged by size: 660 315 34 32 32 32 14 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 67 616 1119 Size: 67 549 503 Fragments arranged by size: 549 503 67 MscI TGG'CCA Cuts at: 0 874 1119 Size: 874 245 MslI CAynn'nnrTG Cuts at: 0 332 743 1119 Size: 332 411 376 Fragments arranged by size: 411 376 332 MspA1I CmG'CkG Cuts at: 0 234 1119 Size: 234 885 NcoI C'CATG_G Cuts at: 0 662 1119 Size: 662 457 NsiI A_TGCA'T Cuts at: 0 534 1119 Size: 534 585 PfoI T'CCnGG_A Cuts at: 0 274 694 1088 1119 Size: 274 420 394 31 Fragments arranged by size: 420 394 274 31 PpuMI rG'GwC_Cy Cuts at: 0 127 1119 Size: 127 992 PsiI TTA'TAA Cuts at: 0 65 1119 Size: 65 1054 PspOMI G'GGCC_C Cuts at: 0 422 1119 Size: 422 697 PstI C_TGCA'G Cuts at: 0 406 1106 1119 Size: 406 700 13 Fragments arranged by size: 700 406 13 PvuII CAG'CTG Cuts at: 0 234 1119 Size: 234 885 SanDI GG'GwC_CC Cuts at: 0 127 1119 Size: 127 992 SexAI A'CCwGG_T Cuts at: 0 262 719 814 1119 Size: 262 457 95 305 Fragments arranged by size: 457 305 262 95 SfcI C'TryA_G Cuts at: 0 402 1102 1119 Size: 402 700 17 Fragments arranged by size: 700 402 17 SmlI C'TyrA_G Cuts at: 0 132 1114 1119 Size: 132 982 5 Fragments arranged by size: 982 132 5 SspI AAT'ATT Cuts at: 0 1012 1119 Size: 1012 107 StyI C'CwwG_G Cuts at: 0 505 662 1057 1119 Size: 505 157 395 62 Fragments arranged by size: 505 395 157 62 TatI w'GTAC_w Cuts at: 0 479 1031 1119 Size: 479 552 88 Fragments arranged by size: 552 479 88 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 503 1055 1119 Size: 503 552 64 Fragments arranged by size: 552 503 64 XhoI C'TCGA_G Cuts at: 0 1114 1119 Size: 1114 5 Enzymes that do cut: AhdI AlfI ApaI ApoI AvaI BamHI BanII BbsI BciVI BfrBI BglII Bme1580I BmrI Bpu10I BpuEI BsaI BsaHI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrDI BsrGI BstEII BstYI BtgI DraI EaeI EarI EciI EcoNI EcoO109I EcoRI FalI HaeII Hin4I MmeI MscI MslI MspA1I NcoI NsiI PfoI PpuMI PsiI PspOMI PstI PvuII SanDI SexAI SfcI SmlI SspI StyI TatI XcmI XhoI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AleI AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BanI BbeI BbvCI BcgI BclI BglI BlpI BmgBI BmtI BplI BpmI BsaAI BsaBI BsaWI BseRI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI EagI EcoICRI Eco57MI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI Tth111I XbaI XmaI XmnI ZraI