(Circular) (Six-base) MAPSORT of: pFN21AE6677.seq Check: 9394 from: 1 to: 5596 pFN21AE6677 5596 bp With 182 enzymes: SgfI * October 17, 2018 20:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2374 2374 Size: 5596 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3406 278 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2939 1937 Size: 1002 4594 Acc65I G'GTAC_C Cuts at: 2172 2917 2172 Size: 745 4851 AclI AA'CG_TT Cuts at: 1563 3723 4096 1563 Size: 2160 373 3063 Fragments arranged by size: 3063 2160 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2171 2249 3659 5043 5311 1632 Size: 539 78 1410 1384 268 1917 Fragments arranged by size: 1917 1410 1384 539 268 78 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5399 AflIII A'CryG_T Cuts at: 1203 2094 4495 5472 1203 Size: 891 2401 977 1327 Fragments arranged by size: 2401 1327 977 891 AgeI A'CCGG_T Cuts at: 4427 4427 Size: 5596 AhdI GACnn_n'nnGTC Cuts at: 4325 4325 Size: 5596 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5596 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5596 AlwNI CAG_nnn'CTG Cuts at: 3339 4911 3339 Size: 1572 4024 ApaLI G'TGCA_C Cuts at: 3653 4809 3653 Size: 1156 4440 ApoI r'AATT_y Cuts at: 1493 2708 2848 2905 3131 3283 5392 1493 Size: 1215 140 57 226 152 2109 1697 Fragments arranged by size: 2109 1697 1215 226 152 140 57 AseI AT'TA_AT Cuts at: 160 1032 4150 160 Size: 872 3118 1606 Fragments arranged by size: 3118 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 AvaI C'yCGr_G Cuts at: 1870 1943 2043 2504 2921 1870 Size: 73 100 461 417 4545 Fragments arranged by size: 4545 461 417 100 73 BamHI G'GATC_C Cuts at: 2926 3293 2926 Size: 367 5229 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2172 2917 4372 618 Size: 325 217 627 385 745 1455 1842 Fragments arranged by size: 1842 1455 745 627 385 325 217 BanII G_rGCy'C Cuts at: 1391 2339 2682 2915 1391 Size: 948 343 233 4072 Fragments arranged by size: 4072 948 343 233 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4625 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3787 3821 1903 Size: 34 1850 34 3678 Fragments arranged by size: 3678 1850 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3488 4698 3488 Size: 1210 4386 BclI T'GATC_A Cuts at: 1548 1548 Size: 5596 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4207 136 Size: 107 122 71 631 605 2535 1525 Fragments arranged by size: 2535 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5591 5591 Size: 5596 BlpI GC'TnA_GC Cuts at: 3013 3013 Size: 5596 Bme1580I G_kGCm'C Cuts at: 1790 3657 4813 1790 Size: 1867 1156 2573 Fragments arranged by size: 2573 1867 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5596 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3327 4285 442 Size: 416 53 83 399 24 1910 958 1753 Fragments arranged by size: 1910 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5596 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2501 4256 1239 Size: 1262 1755 2579 Fragments arranged by size: 2579 1755 1262 Bpu10I CC'TnA_GC Cuts at: 2282 2282 Size: 5596 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2234 3721 4586 4884 2234 Size: 1487 865 298 2946 Fragments arranged by size: 2946 1487 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2564 4259 882 Size: 617 1065 1695 2219 Fragments arranged by size: 2219 1695 1065 617 BsaAI yAC'GTr Cuts at: 493 1204 2218 3387 493 Size: 711 1014 1169 2702 Fragments arranged by size: 2702 1169 1014 711 BsaBI GATnn'nnATC Cuts at: 5590 5590 Size: 5596 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2412 3403 3785 4420 Size: 53 83 186 936 879 991 382 635 Cuts at: 4420 275 Size: 1451 Fragments arranged by size: 1451 991 936 879 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4029 4427 4701 4848 5259 4029 Size: 398 274 147 411 4366 Fragments arranged by size: 4366 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5371 Fragments arranged by size: 5371 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 2138 2213 2270 2284 2522 1186 Size: 871 81 75 57 14 238 4260 Fragments arranged by size: 4260 871 238 81 75 57 14 BseYI C'CCAG_C Cuts at: 4799 5295 4799 Size: 496 5100 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2089 2360 1163 Size: 573 353 271 4399 Fragments arranged by size: 4399 573 353 271 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3046 3807 4835 1540 Size: 275 186 1045 761 1028 2301 Fragments arranged by size: 2301 1045 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2067 2682 3657 3742 4813 2067 Size: 615 975 85 1071 2850 Fragments arranged by size: 2850 1071 975 615 85 BsmI GAATG_Cn' Cuts at: 2410 3107 3200 2410 Size: 697 93 4806 Fragments arranged by size: 4806 697 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2339 2682 2915 3657 3742 4813 Size: 399 277 272 343 233 742 85 1071 Cuts at: 4813 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 343 277 272 233 85 BspEI T'CCGG_A Cuts at: 5259 5259 Size: 5596 BspHI T'CATG_A Cuts at: 2748 3485 5215 2748 Size: 737 1730 3129 Fragments arranged by size: 3129 1730 737 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2374 2951 844 Size: 1530 577 3489 Fragments arranged by size: 3489 1530 577 BsrBI CCG'CTC Cuts at: 1394 1823 3483 1394 Size: 429 1660 3507 Fragments arranged by size: 3507 1660 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4091 4265 65 Size: 1183 2843 174 1396 Fragments arranged by size: 2843 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4240 4427 1700 Size: 110 2430 187 2869 Fragments arranged by size: 2869 2430 187 110 BsrGI T'GTAC_A Cuts at: 96 2091 96 Size: 1995 3601 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5596 BssSI C'ACGA_G Cuts at: 3656 4668 3656 Size: 1012 4584 BstAPI GCAn_nnn'nTGC Cuts at: 794 2842 794 Size: 2048 3548 BstBI TT'CG_AA Cuts at: 4400 4400 Size: 5596 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5596 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5596 BstYI r'GATC_y Cuts at: 1972 2640 2926 3293 3678 3695 5147 5233 5245 Size: 668 286 367 385 17 1452 86 12 Cuts at: 5245 5591 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 668 385 367 346 286 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2157 513 Size: 552 1092 3952 Fragments arranged by size: 3952 1092 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2322 487 Size: 683 56 1096 3761 Fragments arranged by size: 3761 1096 683 56 BtsI GCAGTG_nn' Cuts at: 2135 2445 3112 3906 3926 2135 Size: 310 667 794 20 3805 Fragments arranged by size: 3805 794 667 310 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5596 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2802 2837 529 Size: 35 2238 35 3288 Fragments arranged by size: 3288 2238 35 35 DraI TTT'AAA Cuts at: 2900 2900 Size: 5596 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4603 809 Size: 507 3287 1802 Fragments arranged by size: 3287 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2421 2778 3043 Size: 54 1425 50 275 18 591 357 265 Cuts at: 3043 3932 5561 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 591 357 275 265 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3043 1537 Size: 275 1231 4090 Fragments arranged by size: 4090 1231 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3526 1353 Size: 2173 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4183 4557 4703 387 Size: 1064 138 2594 374 146 1280 Fragments arranged by size: 2594 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2680 2680 Size: 5596 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2249 2501 3659 4256 5043 5311 Size: 393 539 78 252 1158 597 787 268 Cuts at: 5311 1239 Size: 1524 Fragments arranged by size: 1524 1158 787 597 539 393 268 252 78 EcoRI G'AATT_C Cuts at: 2708 2905 3283 2708 Size: 197 378 5021 Fragments arranged by size: 5021 378 197 FspI TGC'GCA Cuts at: 4102 4102 Size: 5596 HaeII r_GCGC'y Cuts at: 1678 4743 1678 Size: 3065 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4237 4269 4311 4343 1373 Size: 32 135 32 2665 32 42 32 2626 Fragments arranged by size: 2665 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2940 3186 669 Size: 988 281 1002 246 3079 Fragments arranged by size: 3079 1002 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5596 HpaI GTT'AAC Cuts at: 3186 3186 Size: 5596 KpnI G_GTAC'C Cuts at: 2176 2921 2176 Size: 745 4851 MfeI C'AATT_G Cuts at: 3195 3195 Size: 5596 MluI A'CGCG_T Cuts at: 5472 5472 Size: 5596 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4710 4894 1305 Size: 3405 184 2007 Fragments arranged by size: 3405 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2423 2780 10 Size: 54 1425 343 591 357 2826 Fragments arranged by size: 2826 1425 591 357 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2703 2753 3554 3913 4072 518 Size: 648 843 694 50 801 359 159 2042 Fragments arranged by size: 2042 843 801 694 648 359 159 50 MspA1I CmG'CkG Cuts at: 1606 2131 2479 3012 3689 4837 5082 1606 Size: 525 348 533 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 533 525 348 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5044 NdeI CA'TA_TG Cuts at: 387 387 Size: 5596 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5596 NotI GC'GGCC_GC Cuts at: 3043 3043 Size: 5596 NspI r_CATG'y Cuts at: 1132 1229 2098 2954 4499 1132 Size: 97 869 856 1545 2229 Fragments arranged by size: 2229 1545 869 856 97 PasI CC'CwG_GG Cuts at: 1348 2142 1348 Size: 794 4802 PciI A'CATG_T Cuts at: 2094 4495 2094 Size: 2401 3195 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5596 PmeI GTTT'AAAC Cuts at: 2900 2900 Size: 5596 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3665 3697 5178 5210 3665 Size: 32 1481 32 4051 Fragments arranged by size: 4051 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5596 PsiI TTA'TAA Cuts at: 3166 3166 Size: 5596 PstI C_TGCA'G Cuts at: 830 2265 2590 2948 830 Size: 1435 325 358 3478 Fragments arranged by size: 3478 1435 358 325 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5596 PvuII CAG'CTG Cuts at: 2131 2479 2131 Size: 348 5248 SacI G_AGCT'C Cuts at: 2682 2682 Size: 5596 SalI G'TCGA_C Cuts at: 1936 2938 1936 Size: 1002 4594 SbfI CC_TGCA'GG Cuts at: 2948 2948 Size: 5596 ScaI AGT'ACT Cuts at: 3844 5556 3844 Size: 1712 3884 SfcI C'TryA_G Cuts at: 826 1045 2261 2462 2586 2944 4079 4760 4951 Size: 219 1216 201 124 358 1135 681 191 Cuts at: 4951 826 Size: 1471 Fragments arranged by size: 1471 1216 1135 681 358 219 201 191 124 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5596 SmaI CCC'GGG Cuts at: 1872 2045 2923 1872 Size: 173 878 4545 Fragments arranged by size: 4545 878 173 SmlI C'TyrA_G Cuts at: 820 1017 1943 2249 3700 4601 4863 820 Size: 197 926 306 1451 901 262 1553 Fragments arranged by size: 1553 1451 926 901 306 262 197 SnaBI TAC'GTA Cuts at: 493 3387 493 Size: 2894 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5596 SphI G_CATG'C Cuts at: 1132 2954 1132 Size: 1822 3774 SspI AAT'ATT Cuts at: 5 52 3520 5 Size: 47 3468 2081 Fragments arranged by size: 3468 2081 47 StuI AGG'CCT Cuts at: 1517 2857 1517 Size: 1340 4256 StyI C'CwwG_G Cuts at: 513 1065 2565 3035 513 Size: 552 1500 470 3074 Fragments arranged by size: 3074 1500 552 470 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3623 3640 3793 3978 4423 325 Size: 1255 2043 17 153 185 445 1498 Fragments arranged by size: 2043 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2091 3842 5554 Size: 274 80 33 51 1443 114 1751 1712 Cuts at: 5554 96 Size: 138 Fragments arranged by size: 1751 1712 1443 274 138 114 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5556 5588 5556 Size: 32 5564 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5596 XbaI T'CTAG_A Cuts at: 2932 2932 Size: 5596 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2483 2752 2483 Size: 269 5327 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5596 XmaI C'CCGG_G Cuts at: 1870 2043 2921 1870 Size: 173 878 4545 Fragments arranged by size: 4545 878 173 XmnI GAAnn'nnTTC Cuts at: 3725 3725 Size: 5596 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3404 276 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI