(Circular) (Six-base) MAPSORT of: pFN21AE2579.seq Check: 5880 from: 1 to: 6370 pFN21AE2579 6370 bp With 182 enzymes: SgfI * June 25, 2015 16:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3478 3478 Size: 6370 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2484 4180 278 Size: 53 83 186 936 948 1696 2468 Fragments arranged by size: 2468 1696 948 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2937 3713 1937 Size: 1000 776 4594 Fragments arranged by size: 4594 1000 776 Acc65I G'GTAC_C Cuts at: 3691 3691 Size: 6370 AclI AA'CG_TT Cuts at: 1563 4497 4870 1563 Size: 2934 373 3063 Fragments arranged by size: 3063 2934 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3173 4433 5817 6085 1632 Size: 1541 1260 1384 268 1917 Fragments arranged by size: 1917 1541 1384 1260 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6173 AflIII A'CryG_T Cuts at: 1203 2296 2857 5269 6246 1203 Size: 1093 561 2412 977 1327 Fragments arranged by size: 2412 1327 1093 977 561 AgeI A'CCGG_T Cuts at: 5201 5201 Size: 6370 AhdI GACnn_n'nnGTC Cuts at: 5099 5099 Size: 6370 AleI CACnn'nnGTG Cuts at: 1166 2297 1166 Size: 1131 5239 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3532 1184 Size: 2348 4022 AlwNI CAG_nnn'CTG Cuts at: 3493 4113 5685 3493 Size: 620 1572 4178 Fragments arranged by size: 4178 1572 620 ApaLI G'TGCA_C Cuts at: 4427 5583 4427 Size: 1156 5214 ApoI r'AATT_y Cuts at: 1493 2748 3679 3905 4057 6166 1493 Size: 1255 931 226 152 2109 1697 Fragments arranged by size: 2109 1697 1255 931 226 152 AseI AT'TA_AT Cuts at: 160 1032 4924 160 Size: 872 3892 1606 Fragments arranged by size: 3892 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6370 AvaI C'yCGr_G Cuts at: 1870 1943 3695 1870 Size: 73 1752 4545 Fragments arranged by size: 4545 1752 73 BamHI G'GATC_C Cuts at: 2328 3700 4067 2328 Size: 1372 367 4631 Fragments arranged by size: 4631 1372 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2564 3691 5146 618 Size: 325 217 627 777 1127 1455 1842 Fragments arranged by size: 1842 1455 1127 777 627 325 217 BanII G_rGCy'C Cuts at: 1391 3045 3689 1391 Size: 1654 644 4072 Fragments arranged by size: 4072 1654 644 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2476 2998 928 Size: 971 577 522 4300 Fragments arranged by size: 4300 971 577 522 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2293 2327 4561 4595 1903 Size: 34 356 34 2234 34 3678 Fragments arranged by size: 3678 2234 356 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4262 5472 4262 Size: 1210 5160 BclI T'GATC_A Cuts at: 1548 2242 2439 1548 Size: 694 197 5479 Fragments arranged by size: 5479 694 197 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2542 2646 4981 Size: 107 122 71 631 605 870 104 2335 Cuts at: 4981 136 Size: 1525 Fragments arranged by size: 2335 1525 870 631 605 122 107 104 71 BglII A'GATC_T Cuts at: 2316 6365 2316 Size: 4049 2321 BlpI GC'TnA_GC Cuts at: 2120 3787 2120 Size: 1667 4703 Bme1580I G_kGCm'C Cuts at: 1790 2567 2594 4431 5587 1790 Size: 777 27 1837 1156 2573 Fragments arranged by size: 2573 1837 1156 777 27 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6370 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4101 5059 442 Size: 416 53 83 399 24 2684 958 1753 Fragments arranged by size: 2684 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6370 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2099 5030 1239 Size: 860 2931 2579 Fragments arranged by size: 2931 2579 860 Bpu10I CC'TnA_GC Cuts at: 2891 2891 Size: 6370 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2365 4495 5360 5658 2365 Size: 2130 865 298 3077 Fragments arranged by size: 3077 2130 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3170 5033 882 Size: 617 1671 1863 2219 Fragments arranged by size: 2219 1863 1671 617 BsaAI yAC'GTr Cuts at: 493 1204 2299 2860 4161 493 Size: 711 1095 561 1301 2702 Fragments arranged by size: 2702 1301 1095 711 561 BsaBI GATnn'nnATC Cuts at: 6364 6364 Size: 6370 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2481 2805 3201 4177 Size: 53 83 186 936 948 324 396 976 Cuts at: 4177 4559 5194 275 Size: 382 635 1451 Fragments arranged by size: 1451 976 948 936 635 396 382 324 186 83 53 BsaWI w'CCGG_w Cuts at: 2197 4803 5201 5475 5622 6033 2197 Size: 2606 398 274 147 411 2534 Fragments arranged by size: 2606 2534 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2176 2206 1178 Size: 30 165 30 773 30 5342 Fragments arranged by size: 5342 773 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2852 1186 Size: 1666 4704 BseYI C'CCAG_C Cuts at: 3283 3292 3493 3590 5573 6069 3283 Size: 9 201 97 1983 496 3584 Fragments arranged by size: 3584 1983 496 201 97 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2753 3506 1163 Size: 573 1017 753 4027 Fragments arranged by size: 4027 1017 753 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2712 3820 4581 5609 1540 Size: 275 186 711 1108 761 1028 2301 Fragments arranged by size: 2301 1108 1028 761 711 275 186 BsiHKAI G_wGCw'C Cuts at: 3045 4431 4516 5587 3045 Size: 1386 85 1071 3828 Fragments arranged by size: 3828 1386 1071 85 BsmI GAATG_Cn' Cuts at: 3881 3974 3881 Size: 93 6277 BsmBI CGTCTCn'nnnn_ Cuts at: 3208 3208 Size: 6370 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2567 2594 3045 3689 4431 4516 5587 Size: 399 777 27 451 644 742 85 1071 Cuts at: 5587 1391 Size: 2174 Fragments arranged by size: 2174 1071 777 742 644 451 399 85 27 BspEI T'CCGG_A Cuts at: 6033 6033 Size: 6370 BspHI T'CATG_A Cuts at: 2346 4259 5989 2346 Size: 1913 1730 2727 Fragments arranged by size: 2727 1913 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2694 3478 3725 844 Size: 1850 784 247 3489 Fragments arranged by size: 3489 1850 784 247 BsrBI CCG'CTC Cuts at: 1394 1823 2192 2998 4257 1394 Size: 429 369 806 1259 3507 Fragments arranged by size: 3507 1259 806 429 369 BsrDI GCAATG_nn' Cuts at: 65 1248 4865 5039 65 Size: 1183 3617 174 1396 Fragments arranged by size: 3617 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3215 5014 5201 1700 Size: 110 1405 1799 187 2869 Fragments arranged by size: 2869 1799 1405 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6370 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6370 BssSI C'ACGA_G Cuts at: 2063 2160 4430 5442 2063 Size: 97 2270 1012 2991 Fragments arranged by size: 2991 2270 1012 97 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6370 BstBI TT'CG_AA Cuts at: 2143 5174 2143 Size: 3031 3339 BstEII G'GTnAC_C Cuts at: 1184 2203 1184 Size: 1019 5351 BstXI CCAn_nnnn'nTGG Cuts at: 2515 2515 Size: 6370 BstYI r'GATC_y Cuts at: 1972 2046 2316 2328 3700 4067 4452 4469 5921 Size: 74 270 12 1372 367 385 17 1452 Cuts at: 5921 6007 6019 6365 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1372 385 367 346 270 86 74 17 12 12 Bsu36I CC'TnA_GG Cuts at: 3079 3079 Size: 6370 BtgI C'CryG_G Cuts at: 513 1065 2004 2107 2583 2712 2721 3207 3545 Size: 552 939 103 476 129 9 486 338 Cuts at: 3545 513 Size: 3338 Fragments arranged by size: 3338 939 552 486 476 338 129 103 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2932 3256 3343 3370 487 Size: 683 56 1706 324 87 27 3487 Fragments arranged by size: 3487 1706 683 324 87 56 27 BtsI GCAGTG_nn' Cuts at: 2537 3447 3886 4680 4700 2537 Size: 910 439 794 20 4207 Fragments arranged by size: 4207 910 794 439 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6370 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6335 DraI TTT'AAA Cuts at: 3674 3674 Size: 6370 DraIII CAC_nnn'GTG Cuts at: 2598 2598 Size: 6370 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5377 809 Size: 507 4061 1802 Fragments arranged by size: 4061 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2709 3213 3817 Size: 54 1425 50 275 18 879 504 604 Cuts at: 3817 4706 6335 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 879 604 504 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2709 3817 1537 Size: 275 897 1108 4090 Fragments arranged by size: 4090 1108 897 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2498 2696 3005 3050 3266 4300 1353 Size: 1145 198 309 45 216 1034 3423 Fragments arranged by size: 3423 1145 1034 309 216 198 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4957 5331 5477 387 Size: 1064 138 3368 374 146 1280 Fragments arranged by size: 3368 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3043 3043 Size: 6370 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 3173 4433 5030 5817 6085 1239 Size: 393 467 1074 1260 597 787 268 1524 Fragments arranged by size: 1524 1260 1074 787 597 467 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2441 2834 2441 Size: 393 5977 EcoO109I rG'GnC_Cy Cuts at: 2434 3370 3512 3526 2434 Size: 936 142 14 5278 Fragments arranged by size: 5278 936 142 14 EcoRI G'AATT_C Cuts at: 3679 4057 3679 Size: 378 5992 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3439 3439 Size: 6370 FspI TGC'GCA Cuts at: 2303 4876 2303 Size: 2573 3797 FspAI rTGC'GCAy Cuts at: 2303 2303 Size: 6370 HaeII r_GCGC'y Cuts at: 1678 3071 5517 1678 Size: 1393 2446 2531 Fragments arranged by size: 2531 2446 1393 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3369 3401 5011 5043 5085 Size: 32 135 32 1797 32 1610 32 42 Cuts at: 5085 5117 1373 Size: 32 2626 Fragments arranged by size: 2626 1797 1610 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3714 3960 669 Size: 988 281 1776 246 3079 Fragments arranged by size: 3079 1776 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6370 HpaI GTT'AAC Cuts at: 3960 3960 Size: 6370 KpnI G_GTAC'C Cuts at: 3695 3695 Size: 6370 MfeI C'AATT_G Cuts at: 3969 3969 Size: 6370 MluI A'CGCG_T Cuts at: 6246 6246 Size: 6370 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2502 5484 5668 1305 Size: 1197 2982 184 2007 Fragments arranged by size: 2982 2007 1197 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4548 Fragments arranged by size: 4548 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2297 2757 4328 4687 4846 518 Size: 648 1131 460 1571 359 159 2042 Fragments arranged by size: 2042 1571 1131 648 460 359 159 MspA1I CmG'CkG Cuts at: 1606 3786 4463 5611 5856 1606 Size: 2180 677 1148 245 2120 Fragments arranged by size: 2180 2120 1148 677 245 NaeI GCC'GGC Cuts at: 3217 3217 Size: 6370 NcoI C'CATG_G Cuts at: 513 1065 2004 2107 513 Size: 552 939 103 4776 Fragments arranged by size: 4776 939 552 103 NdeI CA'TA_TG Cuts at: 387 387 Size: 6370 NgoMIV G'CCGG_C Cuts at: 3215 3215 Size: 6370 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6370 NotI GC'GGCC_GC Cuts at: 3817 3817 Size: 6370 NspI r_CATG'y Cuts at: 1132 1229 3728 5273 1132 Size: 97 2499 1545 2229 Fragments arranged by size: 2499 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2911 1348 Size: 1563 4807 PciI A'CATG_T Cuts at: 5269 5269 Size: 6370 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6370 PfoI T'CCnGG_A Cuts at: 2391 2514 2676 3408 2391 Size: 123 162 732 5353 Fragments arranged by size: 5353 732 162 123 PmeI GTTT'AAAC Cuts at: 3674 3674 Size: 6370 PmlI CAC'GTG Cuts at: 2299 2860 2299 Size: 561 5809 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4439 4471 5952 5984 4439 Size: 32 1481 32 4825 Fragments arranged by size: 4825 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3526 3526 Size: 6370 PshAI GACnn'nnGTC Cuts at: 1613 3242 1613 Size: 1629 4741 PsiI TTA'TAA Cuts at: 3940 3940 Size: 6370 PstI C_TGCA'G Cuts at: 830 2475 3722 830 Size: 1645 1247 3478 Fragments arranged by size: 3478 1645 1247 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6370 SacI G_AGCT'C Cuts at: 3045 3045 Size: 6370 SalI G'TCGA_C Cuts at: 1936 3712 1936 Size: 1776 4594 SanDI GG'GwC_CC Cuts at: 3526 3526 Size: 6370 SapI GCTCTTCn'nnn_ Cuts at: 2696 3005 3050 3266 2696 Size: 309 45 216 5800 Fragments arranged by size: 5800 309 216 45 SbfI CC_TGCA'GG Cuts at: 3722 3722 Size: 6370 ScaI AGT'ACT Cuts at: 4618 6330 4618 Size: 1712 4658 SexAI A'CCwGG_T Cuts at: 3302 3302 Size: 6370 SfcI C'TryA_G Cuts at: 826 1045 2471 3013 3718 4853 5534 5725 826 Size: 219 1426 542 705 1135 681 191 1471 Fragments arranged by size: 1471 1426 1135 705 681 542 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6370 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6370 SmaI CCC'GGG Cuts at: 1872 3697 1872 Size: 1825 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2380 4474 5375 5637 820 Size: 197 926 437 2094 901 262 1553 Fragments arranged by size: 2094 1553 926 901 437 262 197 SnaBI TAC'GTA Cuts at: 493 4161 493 Size: 3668 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6370 SphI G_CATG'C Cuts at: 1132 3728 1132 Size: 2596 3774 SspI AAT'ATT Cuts at: 5 52 4294 5 Size: 47 4242 2081 Fragments arranged by size: 4242 2081 47 StuI AGG'CCT Cuts at: 1517 2267 1517 Size: 750 5620 StyI C'CwwG_G Cuts at: 513 1065 2004 2107 3342 3520 3619 3809 513 Size: 552 939 103 1235 178 99 190 3074 Fragments arranged by size: 3074 1235 939 552 190 178 103 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4397 4414 4567 4752 5197 325 Size: 1255 2817 17 153 185 445 1498 Fragments arranged by size: 2817 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4616 6328 96 Size: 274 80 33 51 1443 2639 1712 138 Fragments arranged by size: 2639 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2176 2208 2451 2483 3550 3582 6330 6362 2176 Size: 32 243 32 1067 32 2748 32 2184 Fragments arranged by size: 2748 2184 1067 243 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2807 1316 Size: 1491 4879 XbaI T'CTAG_A Cuts at: 3706 3706 Size: 6370 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6370 XmaI C'CCGG_G Cuts at: 1870 3695 1870 Size: 1825 4545 XmnI GAAnn'nnTTC Cuts at: 2606 3422 4499 2606 Size: 816 1077 4477 Fragments arranged by size: 4477 1077 816 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2482 4178 276 Size: 53 83 186 936 948 1696 2468 Fragments arranged by size: 2468 1696 948 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I EcoRV FseI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacII SfiI SfoI SrfI SwaI XcmI