################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## Acc65I AclI AcsI AflII AgeI AlwNI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvrII BbrPI BbvCI BcgI BciVI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrBI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI HindIII HpaI KpnI MaeI MamI MfeI MluI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RsrII SacI SalI SapI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 1702 GACGTC GACGT^C AccIII 1268 TCCGGA T^CCGGA AvaII 186 GGTCC G^G[AT]CC BamHI 71 GGATCC G^GATCC BbsI 352 GAAGAC GAAGAC(2/6) BclI 713 TGATCA T^GATCA BglII 872 AGATCT A^GATCT BpuAI 352 GAAGAC GAAGAC(2/6) BsaI 1609 GAGACC GGTCTC(1/5) BseAI 1268 TCCGGA T^CCGGA BsiEI 1715 CGATCG CG[GA][CT]^CG BsmBI 1657 GAGACG CGTCTC(1/5) BspEI 1268 TCCGGA T^CCGGA BspHI 716 TCATGA T^CATGA BsrDI 1101 CATTGC GCAATG(2/0) BsrFI 1207 GCCGGT [GA]^CCGG[CT] BssHII 41 GCGCGC G^CGCGC BstAPI 326 GCACGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 782 CCATTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 1207 GCCGGT [GA]^CCGG[CT] DraI 693 TTTAAA TTT^AAA DraIII 1669 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG FbaI 713 TGATCA T^GATCA HgaI 522 GACGC GACGC(5/10) HincII 931 GTTGAC GT[CT]^[GA]AC HindII 931 GTTGAC GT[CT]^[GA]AC KspI 77 CCGCGG CCGC^GG MroI 1268 TCCGGA T^CCGGA NspI 1749 GCATGT [GA]CATG^[CT] PacI 865 TTAATTAA TTAAT^TAA PleI 133 GACTC GAGTC(4/5) PpuMI 185 GGGTCCT [GA]G^G[AT]CC[CT] PstI 1636 CTGCAG CTGCA^G PvuI 1715 CGATCG CGAT^CG RcaI 716 TCATGA T^CATGA SacII 77 CCGCGG CCGC^GG ScaI 1226 AGTACT AGT^ACT SmaI 1413 CCCGGG CCC^GGG StuI 1378 AGGCCT AGG^CCT XmaCI 1413 CCCGGG C^CCGGG XmaI 1413 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 1015 GTCTAC GT^[AC][GT]AC 1588 GTCTAC AflIII 1509 ACACGT A^C[GA][CT]GT 1660 ACGTGT AhdI 1611 GACCATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1659 GACGTGTCGTC Alw44I 112 GTGCAC G^TGCAC 1154 GTGCAC ApaI 123 GGGCCC GGGCC^C 971 GGGCCC ApaLI 112 GTGCAC G^TGCAC 1154 GTGCAC AspEI 1611 GACCATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1659 GACGTGTCGTC AviII 925 TGCGCA TGC^GCA 1083 TGCGCA BbeI 25 GGCGCC GGCGC^C 82 GGCGCC BsaWI 281 ACCGGA [AT]^CCGG[AT] 1268 TCCGGA BsgI 1881 CTGCAC GTGCAG(16/14) 1696 GTGCAG BstYI 71 GGATCC [GA]^GATC[CT] 872 AGATCT Eam1105I 1611 GACCATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1659 GACGTGTCGTC EarI 541 GAAGAG CTCTTC(1/4) 1519 GAAGAG FspI 925 TGCGCA TGC^GCA 1083 TGCGCA KasI 25 GGCGCC G^GCGCC 82 GGCGCC Ksp632I 541 GAAGAG CTCTTC(1/4) 1519 GAAGAG MflI 71 GGATCC [GA]^GATC[CT] 872 AGATCT NarI 25 GGCGCC GG^CGCC 82 GGCGCC PaeR7I 308 CTCGAG C^TCGAG 1042 CTCGAG SfcI 1636 CTGCAG C^T[GA][CT]AG 1734 CTACAG SfoI 25 GGCGCC GGC^GCC 82 GGCGCC SmlI 308 CTCGAG C^T[CT][GA]AG 1042 CTCGAG Tsp509I 867 AATT ^AATT 1534 AATT XcmI 779 CCACCATTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1792 CCAGTTCTCCGCTGG XhoI 308 CTCGAG C^TCGAG 1042 CTCGAG XhoII 71 GGATCC [GA]^GATC[CT] 872 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AspHI AvaI BalI BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I