Restriction map for
pFN21AE3878
(Circular) (Six-base) MAPSORT of: pFN21AE3878.seq Check: 9259 from: 1 to: 6601
pFN21AE3878 6601 bp
With 182 enzymes: SgfI *
August 4, 2021 21:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3500 3500
Size: 6601
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3711 4411 278
Size: 53 83 186 936 2175 700 2468
Fragments arranged by size:
2468 2175 936 700 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3021 3594 3944 1937
Size: 1084 573 350 4594
Fragments arranged by size:
4594 1084 573 350
Acc65I G'GTAC_C
Cuts at: 3922 3922
Size: 6601
AclI AA'CG_TT
Cuts at: 1563 4728 5101 1563
Size: 3165 373 3063
Fragments arranged by size:
3165 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2217 3481 4664 6048 6316 1632
Size: 585 1264 1183 1384 268 1917
Fragments arranged by size:
1917 1384 1264 1183 585 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6404
AflIII A'CryG_T
Cuts at: 1203 3514 3665 5500 6477 1203
Size: 2311 151 1835 977 1327
Fragments arranged by size:
2311 1835 1327 977 151
AgeI A'CCGG_T
Cuts at: 5432 5432
Size: 6601
AhdI GACnn_n'nnGTC
Cuts at: 3621 3669 5330 3621
Size: 48 1661 4892
Fragments arranged by size:
4892 1661 48
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6601
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6601
AlwNI CAG_nnn'CTG
Cuts at: 4344 5916 4344
Size: 1572 5029
ApaI G_GGCC'C
Cuts at: 2132 2980 2132
Size: 848 5753
ApaLI G'TGCA_C
Cuts at: 2117 3159 4658 5814 2117
Size: 1042 1499 1156 2904
Fragments arranged by size:
2904 1499 1156 1042
ApoI r'AATT_y
Cuts at: 1493 3910 4136 4288 6397 1493
Size: 2417 226 152 2109 1697
Fragments arranged by size:
2417 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5155 160
Size: 872 4123 1606
Fragments arranged by size:
4123 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6601
AvaI C'yCGr_G
Cuts at: 1870 1943 2313 3047 3418 3926 1870
Size: 73 370 734 371 508 4545
Fragments arranged by size:
4545 734 508 371 370 73
BamHI G'GATC_C
Cuts at: 2076 3931 4298 2076
Size: 1855 367 4379
Fragments arranged by size:
4379 1855 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 2087 2804 2864 3077
Size: 325 217 627 243 57 717 60 213
Cuts at: 3077 3922 5377 618
Size: 845 1455 1842
Fragments arranged by size:
1842 1455 845 717 627 325 243 217
213 60 57
BanII G_rGCy'C
Cuts at: 1391 2132 2909 2980 3046 3425 3920 1391
Size: 741 777 71 66 379 495 4072
Fragments arranged by size:
4072 777 741 495 379 71 66
BbeI G_GCGC'C
Cuts at: 2034 2091 2034
Size: 57 6544
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2364 928
Size: 971 465 5165
Fragments arranged by size:
5165 971 465
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4792 4826 1903
Size: 34 2855 34 3678
Fragments arranged by size:
3678 2855 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4493 5703 4493
Size: 1210 5391
BclI T'GATC_A
Cuts at: 1548 2718 1548
Size: 1170 5431
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5212 136
Size: 107 122 71 631 605 3540 1525
Fragments arranged by size:
3540 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2877 6596 2877
Size: 3719 2882
BlpI GC'TnA_GC
Cuts at: 4018 4018
Size: 6601
Bme1580I G_kGCm'C
Cuts at: 1790 2121 2132 2333 2809 2980 3163 4662 5818
Size: 331 11 201 476 171 183 1499 1156
Cuts at: 5818 1790
Size: 2573
Fragments arranged by size:
2573 1499 1156 476 331 201 183 171
11
BmgBI CAC'GTC
Cuts at: 1318 2334 3559 3666 1318
Size: 1016 1225 107 4253
Fragments arranged by size:
4253 1225 1016 107
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3078 4332 5290
Size: 416 53 83 399 24 1661 1254 958
Cuts at: 5290 442
Size: 1753
Fragments arranged by size:
1753 1661 1254 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 2495 2622 3783 5261 1239
Size: 944 312 127 1161 1478 2579
Fragments arranged by size:
2579 1478 1161 944 312 127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4726 5591 5889 4726
Size: 865 298 5438
Fragments arranged by size:
5438 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3608 5264 882
Size: 617 2109 1656 2219
Fragments arranged by size:
2219 2109 1656 617
BsaAI yAC'GTr
Cuts at: 493 1204 4392 493
Size: 711 3188 2702
Fragments arranged by size:
3188 2702 711
BsaBI GATnn'nnATC
Cuts at: 6595 6595
Size: 6601
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2031 2088 3708 4408
Size: 53 83 186 936 498 57 1620 700
Cuts at: 4408 4790 5425 275
Size: 382 635 1451
Fragments arranged by size:
1620 1451 936 700 635 498 382 186
83 57 53
BsaWI w'CCGG_w
Cuts at: 2286 3273 5034 5432 5706 5853 6264 2286
Size: 987 1761 398 274 147 411 2623
Fragments arranged by size:
2623 1761 987 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2903 2933 3650 3680 3786
Size: 30 165 30 1500 30 717 30 106
Cuts at: 3786 3816 1178
Size: 30 3963
Fragments arranged by size:
3963 1500 717 165 106 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2630 2911 3065 3674 3782 3785 1186
Size: 1444 281 154 609 108 3 4002
Fragments arranged by size:
4002 1444 609 281 154 108 3
BseYI C'CCAG_C
Cuts at: 2187 2591 3607 5804 6300 2187
Size: 404 1016 2197 496 2488
Fragments arranged by size:
2488 2197 1016 496 404
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3722 3871 1163
Size: 573 1986 149 3893
Fragments arranged by size:
3893 1986 573 149
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3723 4051 4812 5840 1540
Size: 275 186 1722 328 761 1028 2301
Fragments arranged by size:
2301 1722 1028 761 328 275 186
BsiHKAI G_wGCw'C
Cuts at: 2121 2434 2638 3163 3273 4662 4747 5818 2121
Size: 313 204 525 110 1389 85 1071 2904
Fragments arranged by size:
2904 1389 1071 525 313 204 110 85
BsmI GAATG_Cn'
Cuts at: 4112 4205 4112
Size: 93 6508
BsmBI CGTCTCn'nnnn_
Cuts at: 3656 3656
Size: 6601
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2132 2333 2434 2638 2809 2909
Size: 399 331 11 201 101 204 171 100
Cuts at: 2909 2980 3046 3163 3273 3425 3920 4662 4747
Size: 71 66 117 110 152 495 742 85
Cuts at: 4747 5818 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 495 399 331 204 201
171 152 117 110 101 100 85 71
66 11
BspEI T'CCGG_A
Cuts at: 3273 6264 3273
Size: 2991 3610
BspHI T'CATG_A
Cuts at: 2721 4490 6220 2721
Size: 1769 1730 3102
Fragments arranged by size:
3102 1769 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2357 2973 3500 3956 844
Size: 1513 616 527 456 3489
Fragments arranged by size:
3489 1513 616 527 456
BsrBI CCG'CTC
Cuts at: 1394 1823 4488 1394
Size: 429 2665 3507
Fragments arranged by size:
3507 2665 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3105 5096 5270 65
Size: 1183 1857 1991 174 1396
Fragments arranged by size:
1991 1857 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3212 5245 5432 1700
Size: 110 1402 2033 187 2869
Fragments arranged by size:
2869 2033 1402 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6601
BssHII G'CGCG_C
Cuts at: 1925 2046 1925
Size: 121 6480
BssSI C'ACGA_G
Cuts at: 4661 5673 4661
Size: 1012 5589
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2337 794
Size: 1543 5058
BstBI TT'CG_AA
Cuts at: 5405 5405
Size: 6601
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6601
BstXI CCAn_nnnn'nTGG
Cuts at: 2794 2794
Size: 6601
BstYI r'GATC_y
Cuts at: 1972 2076 2877 3931 4298 4683 4700 6152 6238
Size: 104 801 1054 367 385 17 1452 86
Cuts at: 6238 6250 6596 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1054 801 385 367 346 104
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2082 2479 3505 3618 513
Size: 552 939 78 397 1026 113 3496
Fragments arranged by size:
3496 1026 939 552 397 113 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2379 2420 487
Size: 683 56 1153 41 4668
Fragments arranged by size:
4668 1153 683 56 41
BtsI GCAGTG_nn'
Cuts at: 2760 4117 4911 4931 2760
Size: 1357 794 20 4430
Fragments arranged by size:
4430 1357 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6601
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6566
DraI TTT'AAA
Cuts at: 2700 3905 2700
Size: 1205 5396
DraIII CAC_nnn'GTG
Cuts at: 3679 3679
Size: 6601
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5608 809
Size: 507 4292 1802
Fragments arranged by size:
4292 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2413 2764 3150
Size: 54 1425 50 275 18 583 351 386
Cuts at: 3150 3561 4048 4937 6566 8
Size: 411 487 889 1629 43
Fragments arranged by size:
1629 1425 889 583 487 411 386 351
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4048 1537
Size: 275 2236 4090
Fragments arranged by size:
4090 2236 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2541 3519 4531 1353
Size: 1188 978 1012 3423
Fragments arranged by size:
3423 1188 1012 978
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5188 5562 5708 387
Size: 1064 138 3599 374 146 1280
Fragments arranged by size:
3599 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2217 2495 2622 3481 3783 4664
Size: 393 551 34 278 127 859 302 881
Cuts at: 4664 5261 6048 6316 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 881 859 787 597 551 393 302
278 268 127 34
EcoO109I rG'GnC_Cy
Cuts at: 2128 2129 2191 2976 2977 2128
Size: 1 62 785 1 5752
Fragments arranged by size:
5752 785 62 1 1
EcoRI G'AATT_C
Cuts at: 3910 4288 3910
Size: 378 6223
FspI TGC'GCA
Cuts at: 2932 3090 5107 2932
Size: 158 2017 4426
Fragments arranged by size:
4426 2017 158
FspAI rTGC'GCAy
Cuts at: 3090 3090
Size: 6601
HaeII r_GCGC'y
Cuts at: 1678 2034 2063 2091 2113 3443 5748 1678
Size: 356 29 28 22 1330 2305 2531
Fragments arranged by size:
2531 2305 1330 356 29 28 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2620 2652 2843 2875 2942
Size: 32 135 32 1048 32 191 32 67
Cuts at: 2942 2974 3211 3243 3268 3300 3607 3639 3655
Size: 32 237 32 25 32 307 32 16
Cuts at: 3655 3687 5242 5274 5316 5348 1373
Size: 32 1555 32 42 32 2626
Fragments arranged by size:
2626 1555 1048 307 237 191 135 67
42 32 32 32 32 32 32 32
32 32 32 32 25 16
HincII GTy'rAC
Cuts at: 669 1657 1938 2938 3945 4191 669
Size: 988 281 1000 1007 246 3079
Fragments arranged by size:
3079 1007 1000 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6601
HpaI GTT'AAC
Cuts at: 4191 4191
Size: 6601
KasI G'GCGC_C
Cuts at: 2030 2087 2030
Size: 57 6544
KpnI G_GTAC'C
Cuts at: 3926 3926
Size: 6601
MfeI C'AATT_G
Cuts at: 4200 4200
Size: 6601
MluI A'CGCG_T
Cuts at: 6477 6477
Size: 6601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2529 3322 3381 5715 5899 1305
Size: 1224 793 59 2334 184 2007
Fragments arranged by size:
2334 2007 1224 793 184 59
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2766 3152 3563 10
Size: 54 1425 343 934 386 411 3048
Fragments arranged by size:
3048 1425 934 411 386 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2669 2792 3207 3308 3395 4559 4918
Size: 648 1503 123 415 101 87 1164 359
Cuts at: 4918 5077 518
Size: 159 2042
Fragments arranged by size:
2042 1503 1164 648 415 359 159 123
101 87
MspA1I CmG'CkG
Cuts at: 1606 2084 2135 2762 3058 3646 3807 3885 4017
Size: 478 51 627 296 588 161 78 132
Cuts at: 4017 4694 5842 6087 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 627 588 478 296 245
161 132 78 51
NarI GG'CG_CC
Cuts at: 2031 2088 2031
Size: 57 6544
NcoI C'CATG_G
Cuts at: 513 1065 2004 2479 3505 3618 513
Size: 552 939 475 1026 113 3496
Fragments arranged by size:
3496 1026 939 552 475 113
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6601
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6601
NotI GC'GGCC_GC
Cuts at: 4048 4048
Size: 6601
NspI r_CATG'y
Cuts at: 1132 1229 3758 3959 5504 1132
Size: 97 2529 201 1545 2229
Fragments arranged by size:
2529 2229 1545 201 97
PacI TTA_AT'TAA
Cuts at: 2874 2874
Size: 6601
PasI CC'CwG_GG
Cuts at: 1348 3588 1348
Size: 2240 4361
PciI A'CATG_T
Cuts at: 5500 5500
Size: 6601
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6601
PmeI GTTT'AAAC
Cuts at: 3905 3905
Size: 6601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4670 4702 6183 6215 4670
Size: 32 1481 32 5056
Fragments arranged by size:
5056 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2191 2191
Size: 6601
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6601
PsiI TTA'TAA
Cuts at: 4171 4171
Size: 6601
PspOMI G'GGCC_C
Cuts at: 2128 2976 2128
Size: 848 5753
PspXI vC'TCGA_Gb
Cuts at: 2313 3047 2313
Size: 734 5867
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2717 2749 2717
Size: 32 6569
PstI C_TGCA'G
Cuts at: 830 3645 3953 830
Size: 2815 308 3478
Fragments arranged by size:
3478 2815 308
PvuI CG_AT'CG
Cuts at: 2001 3723 2001
Size: 1722 4879
PvuII CAG'CTG
Cuts at: 3058 3646 3885 3058
Size: 588 239 5774
Fragments arranged by size:
5774 588 239
SacII CC_GC'GG
Cuts at: 2085 2085
Size: 6601
SalI G'TCGA_C
Cuts at: 1936 3943 1936
Size: 2007 4594
SbfI CC_TGCA'GG
Cuts at: 3953 3953
Size: 6601
ScaI AGT'ACT
Cuts at: 3233 4849 6561 3233
Size: 1616 1712 3273
Fragments arranged by size:
3273 1712 1616
SfcI C'TryA_G
Cuts at: 826 1045 3641 3739 3949 5084 5765 5956 826
Size: 219 2596 98 210 1135 681 191 1471
Fragments arranged by size:
2596 1471 1135 681 219 210 191 98
SfoI GGC'GCC
Cuts at: 2032 2089 2032
Size: 57 6544
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6601
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6601
SmaI CCC'GGG
Cuts at: 1872 3420 3928 1872
Size: 1548 508 4545
Fragments arranged by size:
4545 1548 508
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2313 3047 4705 5606 5868 820
Size: 197 926 370 734 1658 901 262 1553
Fragments arranged by size:
1658 1553 926 901 734 370 262 197
SnaBI TAC'GTA
Cuts at: 493 4392 493
Size: 3899 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6601
SphI G_CATG'C
Cuts at: 1132 3959 1132
Size: 2827 3774
SspI AAT'ATT
Cuts at: 5 52 4525 5
Size: 47 4473 2081
Fragments arranged by size:
4473 2081 47
StuI AGG'CCT
Cuts at: 1517 3385 1517
Size: 1868 4733
StyI C'CwwG_G
Cuts at: 513 1065 2004 2403 2479 3165 3505 3618 3874
Size: 552 939 399 76 686 340 113 256
Cuts at: 3874 4040 513
Size: 166 3074
Fragments arranged by size:
3074 939 686 552 399 340 256 166
113 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2621 2868 3178 3887 4628 4645 4798
Size: 1255 1041 247 310 709 741 17 153
Cuts at: 4798 4983 5428 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1041 741 709 445 310 247
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3231 4847 6559
Size: 274 80 33 51 1443 1254 1616 1712
Cuts at: 6559 96
Size: 138
Fragments arranged by size:
1712 1616 1443 1254 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3650 3682 3786 3818 6561 6593 3650
Size: 32 104 32 2743 32 3658
Fragments arranged by size:
3658 2743 104 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6601
XbaI T'CTAG_A
Cuts at: 3937 3937
Size: 6601
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2791 3804 2791
Size: 1013 5588
XhoI C'TCGA_G
Cuts at: 1943 2313 3047 1943
Size: 370 734 5497
Fragments arranged by size:
5497 734 370
XmaI C'CCGG_G
Cuts at: 1870 3418 3926 1870
Size: 1548 508 4545
Fragments arranged by size:
4545 1548 508
XmnI GAAnn'nnTTC
Cuts at: 4730 4730
Size: 6601
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3709 4409 276
Size: 53 83 186 936 2175 700 2468
Fragments arranged by size:
2468 2175 936 700 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PacI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI NaeI NgoMIV NruI NsiI PfoI PmlI RsrII
SacI SanDI SapI SexAI SfiI SrfI SwaI