(Linear) (Six-base) MAPSORT of: KIEE3878.seq Check: 9291 from: 1 to: 1896 KIEE3878.orf With 182 enzymes: SgfI * August 4, 2021 20:20 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1495 1896 Size: 1495 401 AatII G_ACGT'C Cuts at: 0 1706 1896 Size: 1706 190 AccI GT'mk_AC Cuts at: 0 1016 1589 1896 Size: 1016 573 307 Fragments arranged by size: 1016 573 307 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 212 1476 1896 Size: 212 1264 420 Fragments arranged by size: 1264 420 212 AflIII A'CryG_T Cuts at: 0 1509 1660 1896 Size: 1509 151 236 Fragments arranged by size: 1509 236 151 AhdI GACnn_n'nnGTC Cuts at: 0 1616 1664 1896 Size: 1616 48 232 Fragments arranged by size: 1616 232 48 ApaI G_GGCC'C Cuts at: 0 127 975 1896 Size: 127 848 921 Fragments arranged by size: 921 848 127 ApaLI G'TGCA_C Cuts at: 0 112 1154 1896 Size: 112 1042 742 Fragments arranged by size: 1042 742 112 AvaI C'yCGr_G Cuts at: 0 308 1042 1413 1896 Size: 308 734 371 483 Fragments arranged by size: 734 483 371 308 BamHI G'GATC_C Cuts at: 0 71 1896 Size: 71 1825 BanI G'GyrC_C Cuts at: 0 25 82 799 859 1072 1896 Size: 25 57 717 60 213 824 Fragments arranged by size: 824 717 213 60 57 25 BanII G_rGCy'C Cuts at: 0 127 904 975 1041 1420 1896 Size: 127 777 71 66 379 476 Fragments arranged by size: 777 476 379 127 71 66 BbeI G_GCGC'C Cuts at: 0 29 86 1896 Size: 29 57 1810 Fragments arranged by size: 1810 57 29 BbsI GAAGACnn'nnnn_ Cuts at: 0 359 1896 Size: 359 1537 BclI T'GATC_A Cuts at: 0 713 1896 Size: 713 1183 BglII A'GATC_T Cuts at: 0 872 1896 Size: 872 1024 Bme1580I G_kGCm'C Cuts at: 0 116 127 328 804 975 1158 1896 Size: 116 11 201 476 171 183 738 Fragments arranged by size: 738 476 201 183 171 116 11 BmgBI CAC'GTC Cuts at: 0 329 1554 1661 1896 Size: 329 1225 107 235 Fragments arranged by size: 1225 329 235 107 BmrI ACTGGGnnnn_n' Cuts at: 0 1073 1896 Size: 1073 823 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 490 617 1778 1896 Size: 178 312 127 1161 118 Fragments arranged by size: 1161 312 178 127 118 BsaI GGTCTCn'nnnn_ Cuts at: 0 1603 1896 Size: 1603 293 BsaHI Gr'CG_yC Cuts at: 0 26 83 1703 1896 Size: 26 57 1620 193 Fragments arranged by size: 1620 193 57 26 BsaWI w'CCGG_w Cuts at: 0 281 1268 1896 Size: 281 987 628 Fragments arranged by size: 987 628 281 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 898 928 1645 1675 1781 1811 1896 Size: 898 30 717 30 106 30 85 Fragments arranged by size: 898 717 106 85 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 625 906 1060 1669 1777 1780 1896 Size: 625 281 154 609 108 3 116 Fragments arranged by size: 625 609 281 154 116 108 3 BseYI C'CCAG_C Cuts at: 0 182 586 1602 1896 Size: 182 404 1016 294 Fragments arranged by size: 1016 404 294 182 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1717 1866 1896 Size: 1717 149 30 Fragments arranged by size: 1717 149 30 BsiEI CG_ry'CG Cuts at: 0 1718 1896 Size: 1718 178 BsiHKAI G_wGCw'C Cuts at: 0 116 429 633 1158 1268 1896 Size: 116 313 204 525 110 628 Fragments arranged by size: 628 525 313 204 116 110 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1651 1896 Size: 1651 245 Bsp1286I G_dGCh'C Cuts at: 0 116 127 328 429 633 804 904 975 Size: 116 11 201 101 204 171 100 71 Cuts at: 975 1041 1158 1268 1420 1896 Size: 66 117 110 152 476 Fragments arranged by size: 476 204 201 171 152 117 116 110 101 100 71 66 11 BspEI T'CCGG_A Cuts at: 0 1268 1896 Size: 1268 628 BspHI T'CATG_A Cuts at: 0 716 1896 Size: 716 1180 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 352 968 1495 1896 Size: 352 616 527 401 Fragments arranged by size: 616 527 401 352 BsrDI GCAATG_nn' Cuts at: 0 1100 1896 Size: 1100 796 BsrFI r'CCGG_y Cuts at: 0 1207 1896 Size: 1207 689 BssHII G'CGCG_C Cuts at: 0 41 1896 Size: 41 1855 BstAPI GCAn_nnn'nTGC Cuts at: 0 332 1896 Size: 332 1564 BstXI CCAn_nnnn'nTGG Cuts at: 0 789 1896 Size: 789 1107 BstYI r'GATC_y Cuts at: 0 71 872 1896 Size: 71 801 1024 Fragments arranged by size: 1024 801 71 BtgI C'CryG_G Cuts at: 0 77 474 1500 1613 1896 Size: 77 397 1026 113 283 Fragments arranged by size: 1026 397 283 113 77 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 374 415 1896 Size: 374 41 1481 Fragments arranged by size: 1481 374 41 BtsI GCAGTG_nn' Cuts at: 0 755 1896 Size: 755 1141 DraI TTT'AAA Cuts at: 0 695 1896 Size: 695 1201 DraIII CAC_nnn'GTG Cuts at: 0 1674 1896 Size: 1674 222 EaeI y'GGCC_r Cuts at: 0 408 759 1145 1556 1896 Size: 408 351 386 411 340 Fragments arranged by size: 411 408 386 351 340 EarI CTCTTCn'nnn_ Cuts at: 0 536 1514 1896 Size: 536 978 382 Fragments arranged by size: 978 536 382 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 212 490 617 1476 1778 1896 Size: 178 34 278 127 859 302 118 Fragments arranged by size: 859 302 278 178 127 118 34 EcoO109I rG'GnC_Cy Cuts at: 0 123 124 186 971 972 1896 Size: 123 1 62 785 1 924 Fragments arranged by size: 924 785 123 62 1 1 FspI TGC'GCA Cuts at: 0 927 1085 1896 Size: 927 158 811 Fragments arranged by size: 927 811 158 FspAI rTGC'GCAy Cuts at: 0 1085 1896 Size: 1085 811 HaeII r_GCGC'y Cuts at: 0 29 58 86 108 1438 1896 Size: 29 29 28 22 1330 458 Fragments arranged by size: 1330 458 29 29 28 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 615 647 838 870 937 969 1206 1238 Size: 615 32 191 32 67 32 237 32 Cuts at: 1238 1263 1295 1602 1634 1650 1682 1896 Size: 25 32 307 32 16 32 214 Fragments arranged by size: 615 307 237 214 191 67 32 32 32 32 32 32 32 25 16 HincII GTy'rAC Cuts at: 0 933 1896 Size: 933 963 KasI G'GCGC_C Cuts at: 0 25 82 1896 Size: 25 57 1814 Fragments arranged by size: 1814 57 25 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 524 1317 1376 1896 Size: 524 793 59 520 Fragments arranged by size: 793 524 520 59 MscI TGG'CCA Cuts at: 0 761 1147 1558 1896 Size: 761 386 411 338 Fragments arranged by size: 761 411 386 338 MslI CAynn'nnrTG Cuts at: 0 664 787 1202 1303 1390 1896 Size: 664 123 415 101 87 506 Fragments arranged by size: 664 506 415 123 101 87 MspA1I CmG'CkG Cuts at: 0 79 130 757 1053 1641 1802 1880 1896 Size: 79 51 627 296 588 161 78 16 Fragments arranged by size: 627 588 296 161 79 78 51 16 NarI GG'CG_CC Cuts at: 0 26 83 1896 Size: 26 57 1813 Fragments arranged by size: 1813 57 26 NcoI C'CATG_G Cuts at: 0 474 1500 1613 1896 Size: 474 1026 113 283 Fragments arranged by size: 1026 474 283 113 NspI r_CATG'y Cuts at: 0 1753 1896 Size: 1753 143 PacI TTA_AT'TAA Cuts at: 0 869 1896 Size: 869 1027 PasI CC'CwG_GG Cuts at: 0 1583 1896 Size: 1583 313 PpuMI rG'GwC_Cy Cuts at: 0 186 1896 Size: 186 1710 PspOMI G'GGCC_C Cuts at: 0 123 971 1896 Size: 123 848 925 Fragments arranged by size: 925 848 123 PspXI vC'TCGA_Gb Cuts at: 0 308 1042 1896 Size: 308 734 854 Fragments arranged by size: 854 734 308 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 712 744 1896 Size: 712 32 1152 Fragments arranged by size: 1152 712 32 PstI C_TGCA'G Cuts at: 0 1640 1896 Size: 1640 256 PvuI CG_AT'CG Cuts at: 0 1718 1896 Size: 1718 178 PvuII CAG'CTG Cuts at: 0 1053 1641 1880 1896 Size: 1053 588 239 16 Fragments arranged by size: 1053 588 239 16 SacII CC_GC'GG Cuts at: 0 80 1896 Size: 80 1816 ScaI AGT'ACT Cuts at: 0 1228 1896 Size: 1228 668 SfcI C'TryA_G Cuts at: 0 1636 1734 1896 Size: 1636 98 162 Fragments arranged by size: 1636 162 98 SfoI GGC'GCC Cuts at: 0 27 84 1896 Size: 27 57 1812 Fragments arranged by size: 1812 57 27 SmaI CCC'GGG Cuts at: 0 1415 1896 Size: 1415 481 SmlI C'TyrA_G Cuts at: 0 308 1042 1896 Size: 308 734 854 Fragments arranged by size: 854 734 308 StuI AGG'CCT Cuts at: 0 1380 1896 Size: 1380 516 StyI C'CwwG_G Cuts at: 0 398 474 1160 1500 1613 1869 1896 Size: 398 76 686 340 113 256 27 Fragments arranged by size: 686 398 340 256 113 76 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 616 863 1173 1882 1896 Size: 616 247 310 709 14 Fragments arranged by size: 709 616 310 247 14 TatI w'GTAC_w Cuts at: 0 1226 1896 Size: 1226 670 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1645 1677 1781 1813 1896 Size: 1645 32 104 32 83 Fragments arranged by size: 1645 104 83 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 786 1799 1896 Size: 786 1013 97 Fragments arranged by size: 1013 786 97 XhoI C'TCGA_G Cuts at: 0 308 1042 1896 Size: 308 734 854 Fragments arranged by size: 854 734 308 XmaI C'CCGG_G Cuts at: 0 1413 1896 Size: 1413 483 ZraI GAC'GTC Cuts at: 0 1704 1896 Size: 1704 192 Enzymes that do cut: AarI AatII AccI AcuI AflIII AhdI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI BclI BglII Bme1580I BmgBI BmrI BpmI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BssHII BstAPI BstXI BstYI BtgI BtgZI BtsI DraI DraIII EaeI EarI Eco57MI EcoO109I FspI FspAI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI NcoI NspI PacI PasI PpuMI PspOMI PspXI PsrI PstI PvuI PvuII SacII ScaI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XhoI XmaI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AgeI AleI AlfI AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BfrBI BglI BlpI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsiWI BsmI BsrBI BsrGI BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI RsrII SacI SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XmnI