Restriction map for
pFN21AE6547
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# NO CUT ENZYMES ( 199 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DpnII
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FokI FseI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MaeII MamI MboI
MfeI MflI MluI MluNI MroI
MscI MseI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
TaqI Tru9I Tsp45I Tsp509I TspRI
Tth111I TthHB8I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AluI 21 AGCT AG^CT
AspHI 246 GAGCAC G[AT]GC[AT]^C
BbsI 180 GAAGAC GAAGAC(2/6)
BbvI 238 GCAGC GCAGC(8/12)
BfaI 95 CTAG C^TAG
BpmI 132 CTCCAG CTGGAG(16/14)
BpuAI 180 GAAGAC GAAGAC(2/6)
BsiHKAI 246 GAGCAC G[AT]GC[AT]^C
Bsp1407I 221 TGTACA T^GTACA
BspMI 188 ACCTGC ACCTGC(4/8)
BsrGI 221 TGTACA T^GTACA
Cac8I 192 GCCCGC GC[ACGT]^[ACGT]GC
DdeI 211 CTCAG C^T[ACGT]AG
DraII 64 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoNI 108 CCTCACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 64 GGGACCC [GA]G^G[ACGT]CC[CT]
FauI 193 CCCGC CCCGC(4/6)
MaeI 95 CTAG C^TAG
MaeIII 137 GTTAC ^GT[ACGT]AC
MboII 180 GAAGA GAAGA(8/7)
MslI 111 CACCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 194 CCGCTG C[AC]G^C[GT]G
MwoI 151 GCAAAGACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 243 CATG CATG^
PflMI 134 CCAGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 64 GGGACCC [GA]G^G[AT]CC[CT]
SfaNI 90 GATGC GCATC(5/9)
SfcI 197 CTGTAG C^T[GA][CT]AG
SspBI 221 TGTACA T^GTACA
TfiI 124 GAATC G^A[AT]TC
TseI 238 GCAGC G^C[AT]GC
Van91I 134 CCAGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 161 CCGC CCGC(-3/-1)
194 CCGC
AfaI 174 GTAC GT^AC
222 GTAC
AlwNI 135 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
166 CAGAACCTG
AvaII 57 GGTCC G^G[AT]CC
65 GGACC
BmyI 3 GGGCAC G[AGT]GC[ACT]^C
246 GAGCAC
BsaJI 60 CCAGGG C^C[ACGT][ACGT]GG
113 CCCAGG
BsmFI 64 GGGAC GGGAC(10/14)
215 GGGAC
Bsp1286I 3 GGGCAC G[AGT]GC[ACT]^C
246 GAGCAC
BsrI 140 ACTGG ACTGG(1/-1)
134 CCAGT
Cfr13I 57 GGTCC G^G[ACGT]CC
65 GGACC
Fnu4HI 160 GCCGC GC^[ACGT]GC
238 GCAGC
HphI 117 GGTGA GGTGA(8/7)
110 TCACC
ItaI 160 GCCGC GC^[ACGT]GC
238 GCAGC
NlaIV 64 GGGACC GG[ACGT]^[ACGT]CC
65 GGACCC
PleI 11 GACTC GAGTC(4/5)
145 GAGTC
RsaI 174 GTAC GT^AC
222 GTAC
Sau96I 57 GGTCC G^G[ACGT]CC
65 GGACC
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
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BseRI BsiYI BslI BssKI BstNI
EcoRII HinfI MnlI MvaI ScrFI