(Circular) (Six-base) MAPSORT of: pFN21AE6547.seq Check: 6127 from: 1 to: 5008 pFN21AE6547 5008 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2818 278 Size: 53 83 186 936 1282 2468 Fragments arranged by size: 2468 1282 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2351 1937 Size: 414 4594 Acc65I G'GTAC_C Cuts at: 2329 2329 Size: 5008 AclI AA'CG_TT Cuts at: 1563 3135 3508 1563 Size: 1572 373 3063 Fragments arranged by size: 3063 1572 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2315 3071 4455 4723 1632 Size: 683 756 1384 268 1917 Fragments arranged by size: 1917 1384 756 683 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4811 AflIII A'CryG_T Cuts at: 1203 3907 4884 1203 Size: 2704 977 1327 Fragments arranged by size: 2704 1327 977 AgeI A'CCGG_T Cuts at: 3839 3839 Size: 5008 AhdI GACnn_n'nnGTC Cuts at: 3737 3737 Size: 5008 AleI CACnn'nnGTG Cuts at: 1166 2120 1166 Size: 954 4054 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5008 AlwNI CAG_nnn'CTG Cuts at: 2145 2176 2751 4323 2145 Size: 31 575 1572 2830 Fragments arranged by size: 2830 1572 575 31 ApaLI G'TGCA_C Cuts at: 3065 4221 3065 Size: 1156 3852 ApoI r'AATT_y Cuts at: 1493 2317 2543 2695 4804 1493 Size: 824 226 152 2109 1697 Fragments arranged by size: 2109 1697 824 226 152 AseI AT'TA_AT Cuts at: 160 1032 3562 160 Size: 872 2530 1606 Fragments arranged by size: 2530 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5008 AvaI C'yCGr_G Cuts at: 1870 1943 2333 1870 Size: 73 390 4545 Fragments arranged by size: 4545 390 73 BamHI G'GATC_C Cuts at: 2338 2705 2338 Size: 367 4641 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2329 3784 618 Size: 325 217 627 542 1455 1842 Fragments arranged by size: 1842 1455 627 542 325 217 BanII G_rGCy'C Cuts at: 1391 2327 1391 Size: 936 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2192 928 Size: 971 293 3744 Fragments arranged by size: 3744 971 293 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3199 3233 1903 Size: 34 1262 34 3678 Fragments arranged by size: 3678 1262 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2900 4110 2900 Size: 1210 3798 BclI T'GATC_A Cuts at: 1548 1548 Size: 5008 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3619 136 Size: 107 122 71 631 605 1947 1525 Fragments arranged by size: 1947 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5003 5003 Size: 5008 BlpI GC'TnA_GC Cuts at: 2425 2425 Size: 5008 Bme1580I G_kGCm'C Cuts at: 1790 2012 3069 4225 1790 Size: 222 1057 1156 2573 Fragments arranged by size: 2573 1156 1057 222 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5008 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2155 2739 3697 Size: 416 53 83 399 24 738 584 958 Cuts at: 3697 442 Size: 1753 Fragments arranged by size: 1753 958 738 584 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5008 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2122 3668 1239 Size: 883 1546 2579 Fragments arranged by size: 2579 1546 883 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3133 3998 4296 3133 Size: 865 298 3845 Fragments arranged by size: 3845 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3671 882 Size: 617 2172 2219 Fragments arranged by size: 2219 2172 617 BsaAI yAC'GTr Cuts at: 493 1204 2799 493 Size: 711 1595 2702 Fragments arranged by size: 2702 1595 711 BsaBI GATnn'nnATC Cuts at: 5002 5002 Size: 5008 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2815 3197 3832 275 Size: 53 83 186 936 1282 382 635 1451 Fragments arranged by size: 1451 1282 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3441 3839 4113 4260 4671 3441 Size: 398 274 147 411 3778 Fragments arranged by size: 3778 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2004 2034 2292 2322 1178 Size: 30 165 30 601 30 258 30 3864 Fragments arranged by size: 3864 601 258 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2009 2102 2318 1186 Size: 823 93 216 3876 Fragments arranged by size: 3876 823 216 93 BseYI C'CCAG_C Cuts at: 2023 2082 4211 4707 2023 Size: 59 2129 496 2324 Fragments arranged by size: 2324 2129 496 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4435 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2458 3219 4247 1540 Size: 275 186 457 761 1028 2301 Fragments arranged by size: 2301 1028 761 457 275 186 BsiHKAI G_wGCw'C Cuts at: 2255 3069 3154 4225 2255 Size: 814 85 1071 3038 Fragments arranged by size: 3038 1071 814 85 BsmI GAATG_Cn' Cuts at: 2519 2612 2519 Size: 93 4915 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2255 2327 3069 3154 4225 1391 Size: 399 222 243 72 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 243 222 85 72 BspEI T'CCGG_A Cuts at: 4671 4671 Size: 5008 BspHI T'CATG_A Cuts at: 2897 4627 2897 Size: 1730 3278 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2202 2363 844 Size: 1358 161 3489 Fragments arranged by size: 3489 1358 161 BsrBI CCG'CTC Cuts at: 1394 1823 2895 1394 Size: 429 1072 3507 Fragments arranged by size: 3507 1072 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3503 3677 65 Size: 1183 2255 174 1396 Fragments arranged by size: 2255 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3652 3839 1700 Size: 110 1842 187 2869 Fragments arranged by size: 2869 1842 187 110 BsrGI T'GTAC_A Cuts at: 96 2226 96 Size: 2130 2878 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5008 BssSI C'ACGA_G Cuts at: 3068 4080 3068 Size: 1012 3996 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5008 BstBI TT'CG_AA Cuts at: 3812 3812 Size: 5008 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5008 BstYI r'GATC_y Cuts at: 1972 2338 2705 3090 3107 4559 4645 4657 5003 Size: 366 367 385 17 1452 86 12 346 Cuts at: 5003 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 366 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3517 Fragments arranged by size: 3517 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4269 Fragments arranged by size: 4269 683 56 BtsI GCAGTG_nn' Cuts at: 2524 3318 3338 2524 Size: 794 20 4194 Fragments arranged by size: 4194 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5008 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4973 DraI TTT'AAA Cuts at: 2312 2312 Size: 5008 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4015 809 Size: 507 2699 1802 Fragments arranged by size: 2699 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2455 3344 4973 Size: 54 1425 50 275 18 625 889 1629 Cuts at: 4973 8 Size: 43 Fragments arranged by size: 1629 1425 889 625 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2455 1537 Size: 275 643 4090 Fragments arranged by size: 4090 643 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2938 1353 Size: 1585 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3595 3969 4115 387 Size: 1064 138 2006 374 146 1280 Fragments arranged by size: 2006 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2122 2315 3071 3668 4455 4723 1239 Size: 393 490 193 756 597 787 268 1524 Fragments arranged by size: 1524 787 756 597 490 393 268 193 EcoNI CCTnn'n_nnAGG Cuts at: 2117 2117 Size: 5008 EcoO109I rG'GnC_Cy Cuts at: 2070 2070 Size: 5008 EcoRI G'AATT_C Cuts at: 2317 2695 2317 Size: 378 4630 FspI TGC'GCA Cuts at: 3514 3514 Size: 5008 HaeII r_GCGC'y Cuts at: 1678 4155 1678 Size: 2477 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2046 2078 3649 3681 3723 Size: 32 135 32 474 32 1571 32 42 Cuts at: 3723 3755 1373 Size: 32 2626 Fragments arranged by size: 2626 1571 474 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2352 2598 669 Size: 988 281 414 246 3079 Fragments arranged by size: 3079 988 414 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5008 HpaI GTT'AAC Cuts at: 2598 2598 Size: 5008 KpnI G_GTAC'C Cuts at: 2333 2333 Size: 5008 MfeI C'AATT_G Cuts at: 2607 2607 Size: 5008 MluI A'CGCG_T Cuts at: 4884 4884 Size: 5008 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4122 4306 1305 Size: 2817 184 2007 Fragments arranged by size: 2817 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3186 Fragments arranged by size: 3186 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2120 2966 3325 3484 518 Size: 648 954 846 359 159 2042 Fragments arranged by size: 2042 954 846 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2201 2424 3101 4249 4494 1606 Size: 595 223 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 595 245 223 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3517 Fragments arranged by size: 3517 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5008 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5008 NotI GC'GGCC_GC Cuts at: 2455 2455 Size: 5008 NspI r_CATG'y Cuts at: 1132 1229 2366 3911 1132 Size: 97 1137 1545 2229 Fragments arranged by size: 2229 1545 1137 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5008 PciI A'CATG_T Cuts at: 3907 3907 Size: 5008 PflMI CCAn_nnn'nTGG Cuts at: 1108 2145 1108 Size: 1037 3971 PmeI GTTT'AAAC Cuts at: 2312 2312 Size: 5008 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3077 3109 4590 4622 3077 Size: 32 1481 32 3463 Fragments arranged by size: 3463 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2070 2070 Size: 5008 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5008 PsiI TTA'TAA Cuts at: 2578 2578 Size: 5008 PstI C_TGCA'G Cuts at: 830 2360 830 Size: 1530 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5008 SalI G'TCGA_C Cuts at: 1936 2350 1936 Size: 414 4594 SanDI GG'GwC_CC Cuts at: 2070 2070 Size: 5008 SbfI CC_TGCA'GG Cuts at: 2360 2360 Size: 5008 ScaI AGT'ACT Cuts at: 3256 4968 3256 Size: 1712 3296 SfcI C'TryA_G Cuts at: 826 1045 2202 2356 3491 4172 4363 826 Size: 219 1157 154 1135 681 191 1471 Fragments arranged by size: 1471 1157 1135 681 219 191 154 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5008 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5008 SmaI CCC'GGG Cuts at: 1872 2335 1872 Size: 463 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3112 4013 4275 820 Size: 197 926 1169 901 262 1553 Fragments arranged by size: 1553 1169 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2799 493 Size: 2306 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5008 SphI G_CATG'C Cuts at: 1132 2366 1132 Size: 1234 3774 SspI AAT'ATT Cuts at: 5 52 2932 5 Size: 47 2880 2081 Fragments arranged by size: 2880 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5008 StyI C'CwwG_G Cuts at: 513 1065 2004 2447 513 Size: 552 939 443 3074 Fragments arranged by size: 3074 939 552 443 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2132 3035 3052 3205 3390 3835 325 Size: 1255 552 903 17 153 185 445 1498 Fragments arranged by size: 1498 1255 903 552 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2226 3254 4966 Size: 274 80 33 51 1443 249 1028 1712 Cuts at: 4966 96 Size: 138 Fragments arranged by size: 1712 1443 1028 274 249 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2004 2036 4968 5000 2004 Size: 32 2932 32 2012 Fragments arranged by size: 2932 2012 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5008 XbaI T'CTAG_A Cuts at: 2344 2344 Size: 5008 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5008 XmaI C'CCGG_G Cuts at: 1870 2333 1870 Size: 463 4545 XmnI GAAnn'nnTTC Cuts at: 3137 3137 Size: 5008 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2816 276 Size: 53 83 186 936 1282 2468 Fragments arranged by size: 2468 1282 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI