(Circular) (Six-base) MAPSORT of: pFN21AE6679.seq Check: 6268 from: 1 to: 5689 pFN21AE6679 5689 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2245 2915 2245 Size: 670 5019 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3499 278 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2283 3032 1937 Size: 346 749 4594 Fragments arranged by size: 4594 749 346 Acc65I G'GTAC_C Cuts at: 3010 3010 Size: 5689 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2272 2349 3752 5136 5404 1632 Size: 640 77 1403 1384 268 1917 Fragments arranged by size: 1917 1403 1384 640 268 77 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 4588 5565 1203 Size: 3385 977 1327 Fragments arranged by size: 3385 1327 977 AgeI A'CCGG_T Cuts at: 4520 4520 Size: 5689 AhdI GACnn_n'nnGTC Cuts at: 4418 4418 Size: 5689 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5689 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5689 AlwNI CAG_nnn'CTG Cuts at: 3432 5004 3432 Size: 1572 4117 ApaI G_GGCC'C Cuts at: 2849 2903 2912 2849 Size: 54 9 5626 Fragments arranged by size: 5626 54 9 ApaLI G'TGCA_C Cuts at: 3746 4902 3746 Size: 1156 4533 ApoI r'AATT_y Cuts at: 1493 2022 2305 2515 2998 3224 3376 5485 1493 Size: 529 283 210 483 226 152 2109 1697 Fragments arranged by size: 2109 1697 529 483 283 226 210 152 AseI AT'TA_AT Cuts at: 160 1032 2442 4243 160 Size: 872 1410 1801 1606 Fragments arranged by size: 1801 1606 1410 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 2815 2834 2905 2914 3014 1870 Size: 73 872 19 71 9 100 4545 Fragments arranged by size: 4545 872 100 73 71 19 9 BamHI G'GATC_C Cuts at: 3019 3386 3019 Size: 367 5322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3010 4465 618 Size: 325 217 627 1223 1455 1842 Fragments arranged by size: 1842 1455 1223 627 325 217 BanII G_rGCy'C Cuts at: 1391 2849 2903 2912 2950 3008 1391 Size: 1458 54 9 38 58 4072 Fragments arranged by size: 4072 1458 58 54 38 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2015 928 Size: 971 116 4602 Fragments arranged by size: 4602 971 116 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2493 2527 3880 3914 1903 Size: 34 556 34 1353 34 3678 Fragments arranged by size: 3678 1353 556 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3581 4791 3581 Size: 1210 4479 BclI T'GATC_A Cuts at: 1548 1548 Size: 5689 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2907 4300 136 Size: 107 122 71 631 605 1235 1393 1525 Fragments arranged by size: 1525 1393 1235 631 605 122 107 71 BglII A'GATC_T Cuts at: 5684 5684 Size: 5689 BlpI GC'TnA_GC Cuts at: 3106 3106 Size: 5689 Bme1580I G_kGCm'C Cuts at: 1790 2849 2903 2912 3750 4906 1790 Size: 1059 54 9 838 1156 2573 Fragments arranged by size: 2573 1156 1059 838 54 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5689 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2310 2533 3420 Size: 416 53 83 399 24 893 223 887 Cuts at: 3420 4378 442 Size: 958 1753 Fragments arranged by size: 1753 958 893 887 416 399 223 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2874 2934 4349 1239 Size: 1635 60 1415 2579 Fragments arranged by size: 2579 1635 1415 60 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2670 3814 4679 4977 2670 Size: 1144 865 298 3382 Fragments arranged by size: 3382 1144 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4352 882 Size: 617 2853 2219 Fragments arranged by size: 2853 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3480 493 Size: 711 2276 2702 Fragments arranged by size: 2702 2276 711 BsaBI GATnn'nnATC Cuts at: 2720 5683 2720 Size: 2963 2726 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3496 3878 4513 275 Size: 53 83 186 936 1963 382 635 1451 Fragments arranged by size: 1963 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4122 4520 4794 4941 5352 4122 Size: 398 274 147 411 4459 Fragments arranged by size: 4459 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5464 Fragments arranged by size: 5464 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2584 1186 Size: 1398 4291 BseYI C'CCAG_C Cuts at: 2662 2786 4892 5388 2662 Size: 124 2106 496 2963 Fragments arranged by size: 2963 2106 496 124 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2065 1163 Size: 573 329 4787 Fragments arranged by size: 4787 573 329 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3139 3900 4928 1540 Size: 275 186 1138 761 1028 2301 Fragments arranged by size: 2301 1138 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2950 3750 3835 4906 2950 Size: 800 85 1071 3733 Fragments arranged by size: 3733 1071 800 85 BsmI GAATG_Cn' Cuts at: 3200 3293 3200 Size: 93 5596 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2849 2903 2912 2950 3008 3750 3835 Size: 399 1059 54 9 38 58 742 85 Cuts at: 3835 4906 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1059 742 399 85 58 54 38 9 BspEI T'CCGG_A Cuts at: 5352 5352 Size: 5689 BspHI T'CATG_A Cuts at: 2553 3578 5308 2553 Size: 1025 1730 2934 Fragments arranged by size: 2934 1730 1025 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2021 2245 2915 3044 844 Size: 1177 224 670 129 3489 Fragments arranged by size: 3489 1177 670 224 129 BsrBI CCG'CTC Cuts at: 1394 1823 3576 1394 Size: 429 1753 3507 Fragments arranged by size: 3507 1753 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2249 4184 4358 65 Size: 1183 1001 1935 174 1396 Fragments arranged by size: 1935 1396 1183 1001 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4333 4520 1700 Size: 110 2523 187 2869 Fragments arranged by size: 2869 2523 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5689 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5689 BssSI C'ACGA_G Cuts at: 3749 4761 3749 Size: 1012 4677 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5689 BstBI TT'CG_AA Cuts at: 2356 2513 4493 2356 Size: 157 1980 3552 Fragments arranged by size: 3552 1980 157 BstEII G'GTnAC_C Cuts at: 1184 2882 1184 Size: 1698 3991 BstYI r'GATC_y Cuts at: 1972 3019 3386 3771 3788 5240 5326 5338 5684 Size: 1047 367 385 17 1452 86 12 346 Cuts at: 5684 1972 Size: 1977 Fragments arranged by size: 1977 1452 1047 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2895 2895 Size: 5689 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5137 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4950 Fragments arranged by size: 4950 683 56 BtsI GCAGTG_nn' Cuts at: 3205 3999 4019 3205 Size: 794 20 4875 Fragments arranged by size: 4875 794 20 ClaI AT'CG_AT Cuts at: 1554 2868 1554 Size: 1314 4375 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5654 DraI TTT'AAA Cuts at: 2993 2993 Size: 5689 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4696 809 Size: 507 3380 1802 Fragments arranged by size: 3380 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2152 2446 3136 Size: 54 1425 50 275 18 322 294 690 Cuts at: 3136 4025 5654 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 690 322 294 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3136 1537 Size: 275 1324 4090 Fragments arranged by size: 4090 1324 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2212 3619 1353 Size: 859 1407 3423 Fragments arranged by size: 3423 1407 859 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4276 4650 4796 387 Size: 1064 138 2687 374 146 1280 Fragments arranged by size: 2687 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2948 2948 Size: 5689 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2272 2349 2874 2934 3752 4349 5136 Size: 393 640 77 525 60 818 597 787 Cuts at: 5136 5404 1239 Size: 268 1524 Fragments arranged by size: 1524 818 787 640 597 525 393 268 77 60 EcoO109I rG'GnC_Cy Cuts at: 2810 2828 2846 2891 2899 2900 2909 2918 2810 Size: 18 18 45 8 1 9 9 5581 Fragments arranged by size: 5581 45 18 18 9 9 8 1 EcoRI G'AATT_C Cuts at: 2998 3376 2998 Size: 378 5311 FspI TGC'GCA Cuts at: 4195 4195 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 2976 4836 1678 Size: 1298 1860 2531 Fragments arranged by size: 2531 1860 1298 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2085 2117 2168 2200 4330 Size: 32 135 32 513 32 51 32 2130 Cuts at: 4330 4362 4404 4436 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2130 513 135 51 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2593 3033 3279 669 Size: 988 281 655 440 246 3079 Fragments arranged by size: 3079 988 655 440 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5689 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KpnI G_GTAC'C Cuts at: 3014 3014 Size: 5689 MfeI C'AATT_G Cuts at: 2637 3288 2637 Size: 651 5038 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2321 4803 4987 1305 Size: 1016 2482 184 2007 Fragments arranged by size: 2482 2007 1016 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2154 2448 10 Size: 54 1425 343 322 294 3251 Fragments arranged by size: 3251 1425 343 322 294 54 MslI CAynn'nnrTG Cuts at: 518 1166 3647 4006 4165 518 Size: 648 2481 359 159 2042 Fragments arranged by size: 2481 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2075 3105 3782 4930 5175 1606 Size: 469 1030 677 1148 245 2120 Fragments arranged by size: 2120 1148 1030 677 469 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5137 NdeI CA'TA_TG Cuts at: 387 387 Size: 5689 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5689 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 3047 4592 1132 Size: 97 1818 1545 2229 Fragments arranged by size: 2229 1818 1545 97 PasI CC'CwG_GG Cuts at: 1348 2061 1348 Size: 713 4976 PciI A'CATG_T Cuts at: 4588 4588 Size: 5689 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5689 PfoI T'CCnGG_A Cuts at: 2052 2052 Size: 5689 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3758 3790 5271 5303 3758 Size: 32 1481 32 4144 Fragments arranged by size: 4144 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2810 2828 2891 2918 2810 Size: 18 63 27 5581 Fragments arranged by size: 5581 63 27 18 PshAI GACnn'nnGTC Cuts at: 1613 2889 1613 Size: 1276 4413 PsiI TTA'TAA Cuts at: 3259 3259 Size: 5689 PspOMI G'GGCC_C Cuts at: 2845 2899 2908 2845 Size: 54 9 5626 Fragments arranged by size: 5626 54 9 PstI C_TGCA'G Cuts at: 830 2074 2926 3041 830 Size: 1244 852 115 3478 Fragments arranged by size: 3478 1244 852 115 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 PvuII CAG'CTG Cuts at: 2075 2075 Size: 5689 SacI G_AGCT'C Cuts at: 2950 2950 Size: 5689 SalI G'TCGA_C Cuts at: 1936 3031 1936 Size: 1095 4594 SapI GCTCTTCn'nnn_ Cuts at: 2212 2212 Size: 5689 SbfI CC_TGCA'GG Cuts at: 2926 3041 2926 Size: 115 5574 ScaI AGT'ACT Cuts at: 3937 5649 3937 Size: 1712 3977 SfcI C'TryA_G Cuts at: 826 1045 2070 2922 3037 4172 4853 5044 826 Size: 219 1025 852 115 1135 681 191 1471 Fragments arranged by size: 1471 1135 1025 852 681 219 191 115 SfiI GGCCn_nnn'nGGCC Cuts at: 2907 2907 Size: 5689 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5689 SmaI CCC'GGG Cuts at: 1872 2817 2836 2907 2916 3016 1872 Size: 945 19 71 9 100 4545 Fragments arranged by size: 4545 945 100 71 19 9 SmlI C'TyrA_G Cuts at: 820 1017 1943 2685 3793 4694 4956 820 Size: 197 926 742 1108 901 262 1553 Fragments arranged by size: 1553 1108 926 901 742 262 197 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 3047 1132 Size: 1915 3774 SspI AAT'ATT Cuts at: 5 52 3613 5 Size: 47 3561 2081 Fragments arranged by size: 3561 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5689 StyI C'CwwG_G Cuts at: 513 1065 3128 513 Size: 552 2063 3074 Fragments arranged by size: 3074 2063 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2901 3716 3733 3886 4071 4516 325 Size: 1255 1321 815 17 153 185 445 1498 Fragments arranged by size: 1498 1321 1255 815 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3935 5647 96 Size: 274 80 33 51 1443 1958 1712 138 Fragments arranged by size: 1958 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5649 5681 5649 Size: 32 5657 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5689 XbaI T'CTAG_A Cuts at: 2213 3025 2213 Size: 812 4877 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5689 XmaI C'CCGG_G Cuts at: 1870 2815 2834 2905 2914 3014 1870 Size: 945 19 71 9 100 4545 Fragments arranged by size: 4545 945 100 71 19 9 XmnI GAAnn'nnTTC Cuts at: 3818 3818 Size: 5689 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3497 276 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI XcmI