################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstNI BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRII EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MslI MunI MvaI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TspRI Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AocI 184 CCTAAGG CC^T[ACGT]AGG AspI 115 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 6 GGCGCC G^G[CT][GA]CC BanII 43 GGGCTC G[GA]GC[CT]^C BbeI 6 GGCGCC GGCGC^C BcgI 109 GCATTTGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 147 CCGGG CC^[GC]GG BmyI 43 GGGCTC G[AGT]GC[ACT]^C BpmI 450 CTGGAG CTGGAG(16/14) BseRI 193 GAGGAG GAGGAG(10/8) BsmAI 378 GAGAC GTCTC(1/5) Bsp1286I 43 GGGCTC G[AGT]GC[ACT]^C Bsp1407I 298 TGTACA T^GTACA BspHI 918 TCATGA T^CATGA BsrGI 298 TGTACA T^GTACA BssKI 147 CCGGG ^CC[ACGT]GG BstAPI 778 GCAACTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 249 GGATG GGATG(2/0) BstXI 68 CCATGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 184 CCTAAGG CC^T[ACGT]AGG DsaI 68 CCATGG C^C[GA][CT]GG EaeI 77 TGGCCG [CT]^GGCC[GA] Eco81I 184 CCTAAGG CC^T[ACGT]AGG EcoRV 872 GATATC GAT^ATC FauI 48 CCCGC CCCGC(4/6) FokI 249 GGATG GGATG(9/13) HaeII 6 GGCGCC [GA]GCGC^[CT] HgaI 136 GCGTC GACGC(5/10) HincII 159 GTTGAC GT[CT]^[GA]AC HindII 159 GTTGAC GT[CT]^[GA]AC KasI 6 GGCGCC G^GCGCC NarI 6 GGCGCC GG^CGCC NciI 147 CCGGG CC^[GC]GG NcoI 68 CCATGG C^CATGG PflFI 115 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 31 AGGACCC [GA]G^G[AT]CC[CT] RcaI 918 TCATGA T^CATGA ScrFI 147 CCGGG CC^[ACGT]GG SfcI 788 CTACAG C^T[GA][CT]AG SfoI 6 GGCGCC GGC^GCC SspBI 298 TGTACA T^GTACA TaqI 270 TCGA T^CGA Tth111I 115 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 270 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcyI 6 GGCGCC G[GA]^CG[CT]C 135 GGCGTC AvaII 32 GGACC G^G[AT]CC 92 GGACC BglII 627 AGATCT A^GATCT 901 AGATCT BsaHI 6 GGCGCC G[GA]^CG[CT]C 135 GGCGTC BsaJI 68 CCATGG C^C[ACGT][ACGT]GG 74 CCTTGG BsiYI 35 CCCAACTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 488 CCTCTTCATGG BslI 35 CCCAACTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 488 CCTCTTCATGG BsmFI 91 GGGAC GGGAC(10/14) 396 GTCCC BstYI 627 AGATCT [GA]^GATC[CT] 901 AGATCT Cac8I 221 GCAGGC GC[ACGT]^[ACGT]GC 334 GCTTGC CfoI 7 GCGC GCG^C 652 GCGC DraII 31 AGGACCC [GA]G^G[ACGT]CC[CT] 930 AGGGCCT EarI 489 CTCTTC CTCTTC(1/4) 190 GAAGAG EcoO109I 31 AGGACCC [GA]G^G[ACGT]CC[CT] 930 AGGGCCT EcoT14I 68 CCATGG C^C[AT][AT]GG 74 CCTTGG HapII 80 CCGG C^CGG 147 CCGG HhaI 7 GCGC GCG^C 652 GCGC Hin1I 6 GGCGCC G[GA]^CG[CT]C 135 GGCGTC HinP1I 7 GCGC G^CGC 652 GCGC HpaII 80 CCGG C^CGG 147 CCGG Ksp632I 489 CTCTTC CTCTTC(1/4) 190 GAAGAG MaeIII 455 GTGAC ^GT[ACGT]AC 836 GTGAC MflI 627 AGATCT [GA]^GATC[CT] 901 AGATCT MspI 80 CCGG C^CGG 147 CCGG NspI 205 GCATGT [GA]CATG^[CT] 295 GCATGT PleI 115 GACTC GAGTC(4/5) 466 GACTC PvuII 358 CAGCTG CAG^CTG 791 CAGCTG SfaNI 402 GATGC GCATC(5/9) 698 GATGC StyI 68 CCATGG C^C[AT][AT]GG 74 CCTTGG Tsp45I 455 GTGAC ^GT[GC]AC 836 GTGAC XhoII 627 AGATCT [GA]^GATC[CT] 901 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BbvI BsrI Cfr13I DdeI DpnII Fnu4HI HaeIII HinfI HphI ItaI MboI MboII MnlI MseI MspA1I MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I TfiI Tru9I TseI Tsp509I