Restriction map for
pFN21AE2675
(Linear) (Six-base) MAPSORT of: KIEE2675.seq Check: 2095 from: 1 to: 972
KIEE2675.orf
With 182 enzymes: SgfI *
February 2, 2017 14:35 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 932 972
Size: 125 807 40
Fragments arranged by size:
807 125 40
ApoI r'AATT_y
Cuts at: 0 591 642 660 972
Size: 591 51 18 312
Fragments arranged by size:
591 312 51 18
BanI G'GyrC_C
Cuts at: 0 6 972
Size: 6 966
BanII G_rGCy'C
Cuts at: 0 47 972
Size: 47 925
BbeI G_GCGC'C
Cuts at: 0 10 972
Size: 10 962
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 98 132 972
Size: 98 34 840
Fragments arranged by size:
840 98 34
BglII A'GATC_T
Cuts at: 0 627 901 972
Size: 627 274 71
Fragments arranged by size:
627 274 71
BmrI ACTGGGnnnn_n'
Cuts at: 0 49 972
Size: 49 923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 471 972
Size: 471 501
BsaHI Gr'CG_yC
Cuts at: 0 7 136 972
Size: 7 129 836
Fragments arranged by size:
836 129 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 29 59 972
Size: 29 30 913
Fragments arranged by size:
913 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 208 972
Size: 208 764
Bsp1286I G_dGCh'C
Cuts at: 0 47 972
Size: 47 925
BspHI T'CATG_A
Cuts at: 0 918 972
Size: 918 54
BsrGI T'GTAC_A
Cuts at: 0 298 972
Size: 298 674
BstAPI GCAn_nnn'nTGC
Cuts at: 0 784 972
Size: 784 188
BstXI CCAn_nnnn'nTGG
Cuts at: 0 75 972
Size: 75 897
BstYI r'GATC_y
Cuts at: 0 627 901 972
Size: 627 274 71
Fragments arranged by size:
627 274 71
Bsu36I CC'TnA_GG
Cuts at: 0 185 972
Size: 185 787
BtgI C'CryG_G
Cuts at: 0 68 972
Size: 68 904
EaeI y'GGCC_r
Cuts at: 0 77 972
Size: 77 895
EarI CTCTTCn'nnn_
Cuts at: 0 185 495 972
Size: 185 310 477
Fragments arranged by size:
477 310 185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 471 932 972
Size: 125 346 461 40
Fragments arranged by size:
461 346 125 40
EcoO109I rG'GnC_Cy
Cuts at: 0 32 931 972
Size: 32 899 41
Fragments arranged by size:
899 41 32
EcoRV GAT'ATC
Cuts at: 0 874 972
Size: 874 98
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 406 970 972
Size: 406 564 2
Fragments arranged by size:
564 406 2
HaeII r_GCGC'y
Cuts at: 0 10 972
Size: 10 962
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 121 153 489 521 972
Size: 121 32 336 32 451
Fragments arranged by size:
451 336 121 32 32
HincII GTy'rAC
Cuts at: 0 161 972
Size: 161 811
KasI G'GCGC_C
Cuts at: 0 6 972
Size: 6 966
MspA1I CmG'CkG
Cuts at: 0 54 360 793 972
Size: 54 306 433 179
Fragments arranged by size:
433 306 179 54
NarI GG'CG_CC
Cuts at: 0 7 972
Size: 7 965
NcoI C'CATG_G
Cuts at: 0 68 972
Size: 68 904
NspI r_CATG'y
Cuts at: 0 209 299 972
Size: 209 90 673
Fragments arranged by size:
673 209 90
PpuMI rG'GwC_Cy
Cuts at: 0 32 972
Size: 32 940
PvuII CAG'CTG
Cuts at: 0 360 793 972
Size: 360 433 179
Fragments arranged by size:
433 360 179
SfcI C'TryA_G
Cuts at: 0 788 972
Size: 788 184
SfoI GGC'GCC
Cuts at: 0 8 972
Size: 8 964
StyI C'CwwG_G
Cuts at: 0 68 74 972
Size: 68 6 898
Fragments arranged by size:
898 68 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 15 108 115 707 972
Size: 15 93 7 592 265
Fragments arranged by size:
592 265 93 15 7
TatI w'GTAC_w
Cuts at: 0 298 972
Size: 298 674
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 759 791 972
Size: 759 32 181
Fragments arranged by size:
759 181 32
Tth111I GACn'n_nGTC
Cuts at: 0 118 972
Size: 118 854
Enzymes that do cut:
AcuI ApoI BanI BanII BbeI BcgI BglII BmrI
BpmI BsaHI BsaXI BseRI Bsp1286I BspHI BsrGI BstAPI
BstXI BstYI Bsu36I BtgI EaeI EarI Eco57MI EcoO109I
EcoRV FalI HaeII Hin4I HincII KasI MspA1I NarI
NcoI NspI PpuMI PvuII SfcI SfoI StyI TaqII
TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbsI
BbvCI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI XbaI XcmI XhoI XmaI XmnI
ZraI