################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII BalI BamHI BbeI BbrPI BbvCI BcgI BfrI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HgaI Hin1I HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AclI 962 AACGTT AA^CGTT Aor51HI 127 AGCGCT AGC^GCT Asp700I 737 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 915 GAGCAC G[AT]GC[AT]^C AvaI 123 CCCGAG C^[CT]CG[GA]G BciVI 636 GGATAC GTATCC(6/5) BclI 1416 TGATCA T^GATCA BglI 41 GCCCCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 518 CTGGAG CTGGAG(16/14) BsgI 503 CTGCAC GTGCAG(16/14) BsiHKAI 915 GAGCAC G[AT]GC[AT]^C BsmI 741 GCATTC GAATGC(1/-1) BsoBI 123 CCCGAG C^[CT]CG[GA]G BspHI 1422 TCATGA T^CATGA BsrBI 1098 CCGCTC CCGCTC(-3/-3) BssSI 227 CTCGTG CACGAG(-5/-1) DraII 39 GGGCCCC [GA]G^G[ACGT]CC[CT] Eco47III 127 AGCGCT AGC^GCT EcoO109I 39 GGGCCCC [GA]G^G[ACGT]CC[CT] FbaI 1416 TGATCA T^GATCA HincII 301 GTTGAC GT[CT]^[GA]AC HindII 301 GTTGAC GT[CT]^[GA]AC MseI 242 TTAA T^TAA MslI 1434 CATGGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 45 CAGCGG C[AC]G^C[GT]G NdeI 326 CATATG CA^TATG NheI 130 GCTAGC G^CTAGC Psp1406I 962 AACGTT AA^CGTT RcaI 1422 TCATGA T^CATGA ScaI 574 AGTACT AGT^ACT Tru9I 242 TTAA T^TAA XmnI 737 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwNI 277 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG 556 CAGTTGCTG ApaI 39 GGGCCC GGGCC^C 52 GGGCCC AvaII 34 GGTCC G^G[AT]CC 1218 GGACC AvrII 165 CCTAGG C^CTAGG 1111 CCTAGG BanI 91 GGCACC G^G[CT][GA]CC 375 GGCACC BglII 880 AGATCT A^GATCT 1057 AGATCT BlnI 165 CCTAGG C^CTAGG 1111 CCTAGG BsaBI 749 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1414 GATGATCATC BsaI 610 GGTCTC GGTCTC(1/5) 1076 GGTCTC BseRI 768 GAGGAG GAGGAG(10/8) 771 GAGGAG BsmBI 819 GAGACG CGTCTC(1/5) 1026 GAGACG BspMI 31 GCAGGT ACCTGC(4/8) 394 GCAGGT BstYI 880 AGATCT [GA]^GATC[CT] 1057 AGATCT HaeII 127 AGCGCT [GA]GCGC^[CT] 451 GGCGCT HaeIII 40 GGCC GG^CC 53 GGCC HindIII 359 AAGCTT A^AGCTT 565 AAGCTT MamI 749 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1414 GATGATCATC MflI 880 AGATCT [GA]^GATC[CT] 1057 AGATCT PshAI 111 GACTAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 821 GACGGCGGTC PstI 29 CTGCAG CTGCA^G 548 CTGCAG SfaNI 144 GATGC GCATC(5/9) 279 GCATC TaqI 176 TCGA T^CGA 817 TCGA Tsp509I 696 AATT ^AATT 1091 AATT TthHB8I 176 TCGA T^CGA 817 TCGA XbaI 790 TCTAGA T^CTAGA 1006 TCTAGA XhoII 880 AGATCT [GA]^GATC[CT] 1057 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AfaI AluI AlwI BanII BbsI BbvI BcnI BfaI BmyI BpuAI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII EcoT14I FauI Fnu4HI FokI HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfcI StyI TfiI TseI Tsp45I TspRI