(Circular) (Six-base) MAPSORT of: pFN21AE5657.seq Check: 2410 from: 1 to: 6154 pFN21AE5657 6154 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3964 278 Size: 53 83 186 936 2428 2468 Fragments arranged by size: 2468 2428 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3497 1937 Size: 1560 4594 Acc65I G'GTAC_C Cuts at: 3475 3475 Size: 6154 AclI AA'CG_TT Cuts at: 1563 2968 4281 4654 1563 Size: 1405 1313 373 3063 Fragments arranged by size: 3063 1405 1313 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2588 4217 5601 5869 1632 Size: 956 1629 1384 268 1917 Fragments arranged by size: 1917 1629 1384 956 268 AfeI AGC'GCT Cuts at: 2134 2134 Size: 6154 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5957 AflIII A'CryG_T Cuts at: 1203 5053 6030 1203 Size: 3850 977 1327 Fragments arranged by size: 3850 1327 977 AgeI A'CCGG_T Cuts at: 4985 4985 Size: 6154 AhdI GACnn_n'nnGTC Cuts at: 4883 4883 Size: 6154 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6154 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6154 AlwNI CAG_nnn'CTG Cuts at: 2287 2566 3897 5469 2287 Size: 279 1331 1572 2972 Fragments arranged by size: 2972 1572 1331 279 ApaI G_GGCC'C Cuts at: 2048 2061 2048 Size: 13 6141 ApaLI G'TGCA_C Cuts at: 4211 5367 4211 Size: 1156 4998 ApoI r'AATT_y Cuts at: 1493 3463 3689 3841 5950 1493 Size: 1970 226 152 2109 1697 Fragments arranged by size: 2109 1970 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4708 160 Size: 872 3676 1606 Fragments arranged by size: 3676 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6154 AvaI C'yCGr_G Cuts at: 1870 1943 2128 3479 1870 Size: 73 185 1351 4545 Fragments arranged by size: 4545 1351 185 73 AvrII C'CTAG_G Cuts at: 2170 3116 2170 Size: 946 5208 BamHI G'GATC_C Cuts at: 3484 3851 3484 Size: 367 5787 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2096 2380 3475 4930 618 Size: 325 217 627 309 284 1095 1455 1842 Fragments arranged by size: 1842 1455 1095 627 325 309 284 217 BanII G_rGCy'C Cuts at: 1391 2048 2061 2485 3473 1391 Size: 657 13 424 988 4072 Fragments arranged by size: 4072 988 657 424 13 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2396 2958 3441 928 Size: 971 497 562 483 3641 Fragments arranged by size: 3641 971 562 497 483 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4345 4379 1903 Size: 34 2408 34 3678 Fragments arranged by size: 3678 2408 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2635 4046 5256 2635 Size: 1411 1210 3533 Fragments arranged by size: 3533 1411 1210 BclI T'GATC_A Cuts at: 1548 3421 1548 Size: 1873 4281 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2052 4765 136 Size: 107 122 71 631 605 380 2713 1525 Fragments arranged by size: 2713 1525 631 605 380 122 107 71 BglII A'GATC_T Cuts at: 2885 3062 6149 2885 Size: 177 3087 2890 Fragments arranged by size: 3087 2890 177 BlpI GC'TnA_GC Cuts at: 3571 3571 Size: 6154 Bme1580I G_kGCm'C Cuts at: 1790 2048 2061 4215 5371 1790 Size: 258 13 2154 1156 2573 Fragments arranged by size: 2573 2154 1156 258 13 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6154 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3885 4843 442 Size: 416 53 83 399 24 2468 958 1753 Fragments arranged by size: 2468 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2139 1056 Size: 1083 5071 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2799 2904 2799 Size: 105 6049 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2544 4814 1239 Size: 1305 2270 2579 Fragments arranged by size: 2579 2270 1305 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4279 5144 5442 4279 Size: 865 298 4991 Fragments arranged by size: 4991 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2621 3087 4817 882 Size: 617 1122 466 1730 2219 Fragments arranged by size: 2219 1730 1122 617 466 BsaAI yAC'GTr Cuts at: 493 1204 3945 493 Size: 711 2741 2702 Fragments arranged by size: 2741 2702 711 BsaBI GATnn'nnATC Cuts at: 2758 3423 6148 2758 Size: 665 2725 2764 Fragments arranged by size: 2764 2725 665 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3961 4343 4978 275 Size: 53 83 186 936 2428 382 635 1451 Fragments arranged by size: 2428 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4587 4985 5259 5406 5817 4587 Size: 398 274 147 411 4924 Fragments arranged by size: 4924 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3049 3079 1178 Size: 30 165 30 1646 30 4253 Fragments arranged by size: 4253 1646 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2788 2791 1186 Size: 1602 3 4549 Fragments arranged by size: 4549 1602 3 BseYI C'CCAG_C Cuts at: 2027 2048 5357 5853 2027 Size: 21 3309 496 2328 Fragments arranged by size: 3309 2328 496 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2493 1163 Size: 573 757 4824 Fragments arranged by size: 4824 757 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3604 4365 5393 1540 Size: 275 186 1603 761 1028 2301 Fragments arranged by size: 2301 1603 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2924 4215 4300 5371 2924 Size: 1291 85 1071 3707 Fragments arranged by size: 3707 1291 1071 85 BsmI GAATG_Cn' Cuts at: 2746 3665 3758 2746 Size: 919 93 5142 Fragments arranged by size: 5142 919 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2818 3025 2818 Size: 207 5947 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2061 2485 2924 3473 4215 4300 Size: 399 258 13 424 439 549 742 85 Cuts at: 4300 5371 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 549 439 424 399 258 85 13 BspEI T'CCGG_A Cuts at: 5817 5817 Size: 6154 BspHI T'CATG_A Cuts at: 3427 4043 5773 3427 Size: 616 1730 3808 Fragments arranged by size: 3808 1730 616 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2027 2390 3509 844 Size: 1183 363 1119 3489 Fragments arranged by size: 3489 1183 1119 363 BsrBI CCG'CTC Cuts at: 1394 1823 3105 4041 1394 Size: 429 1282 936 3507 Fragments arranged by size: 3507 1282 936 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4649 4823 65 Size: 1183 3401 174 1396 Fragments arranged by size: 3401 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4798 4985 1700 Size: 110 2988 187 2869 Fragments arranged by size: 2988 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6154 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6154 BssSI C'ACGA_G Cuts at: 2232 4214 5226 2232 Size: 1982 1012 3160 Fragments arranged by size: 3160 1982 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6154 BstBI TT'CG_AA Cuts at: 4958 4958 Size: 6154 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6154 BstYI r'GATC_y Cuts at: 1972 2885 3062 3484 3851 4236 4253 5705 5791 Size: 913 177 422 367 385 17 1452 86 Cuts at: 5791 5803 6149 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 913 422 385 367 346 177 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5602 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5415 Fragments arranged by size: 5415 683 56 BtsI GCAGTG_nn' Cuts at: 2031 2400 3670 4464 4484 2031 Size: 369 1270 794 20 3701 Fragments arranged by size: 3701 1270 794 369 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6154 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6119 DraI TTT'AAA Cuts at: 3458 3458 Size: 6154 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5161 809 Size: 507 3845 1802 Fragments arranged by size: 3845 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3601 4490 6119 Size: 54 1425 50 275 18 1771 889 1629 Cuts at: 6119 8 Size: 43 Fragments arranged by size: 1771 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3601 1537 Size: 275 1789 4090 Fragments arranged by size: 4090 1789 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2165 2201 3197 3276 3315 4084 1353 Size: 812 36 996 79 39 769 3423 Fragments arranged by size: 3423 996 812 769 79 39 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2082 2100 2893 4741 5115 5261 Size: 1064 138 493 18 793 1848 374 146 Cuts at: 5261 387 Size: 1280 Fragments arranged by size: 1848 1280 1064 793 493 374 146 138 18 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2544 2588 4217 4814 5601 5869 1239 Size: 393 912 44 1629 597 787 268 1524 Fragments arranged by size: 1629 1524 912 787 597 393 268 44 EcoO109I rG'GnC_Cy Cuts at: 2045 2045 Size: 6154 EcoRI G'AATT_C Cuts at: 3463 3841 3463 Size: 378 5776 FspI TGC'GCA Cuts at: 4660 4660 Size: 6154 HaeII r_GCGC'y Cuts at: 1678 2136 2460 5301 1678 Size: 458 324 2841 2531 Fragments arranged by size: 2841 2531 458 324 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2140 2172 2249 2281 4795 Size: 32 135 32 568 32 77 32 2514 Cuts at: 4795 4827 4869 4901 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2514 568 135 77 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2308 3498 3744 669 Size: 988 281 370 1190 246 3079 Fragments arranged by size: 3079 1190 988 370 281 246 HindIII A'AGCT_T Cuts at: 748 2364 2570 748 Size: 1616 206 4332 Fragments arranged by size: 4332 1616 206 HpaI GTT'AAC Cuts at: 3744 3744 Size: 6154 KpnI G_GTAC'C Cuts at: 3479 3479 Size: 6154 MfeI C'AATT_G Cuts at: 3753 3753 Size: 6154 MluI A'CGCG_T Cuts at: 6030 6030 Size: 6154 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5268 5452 1305 Size: 3963 184 2007 Fragments arranged by size: 3963 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4332 Fragments arranged by size: 4332 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3443 4112 4471 4630 518 Size: 648 2277 669 359 159 2042 Fragments arranged by size: 2277 2042 669 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2052 3570 4247 5395 5640 1606 Size: 446 1518 677 1148 245 2120 Fragments arranged by size: 2120 1518 1148 677 446 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5602 NdeI CA'TA_TG Cuts at: 387 2332 387 Size: 1945 4209 NheI G'CTAG_C Cuts at: 1052 2135 1052 Size: 1083 5071 NotI GC'GGCC_GC Cuts at: 3601 3601 Size: 6154 NspI r_CATG'y Cuts at: 1132 1229 3512 5057 1132 Size: 97 2283 1545 2229 Fragments arranged by size: 2283 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6154 PciI A'CATG_T Cuts at: 5053 5053 Size: 6154 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6154 PfoI T'CCnGG_A Cuts at: 2521 2521 Size: 6154 PmeI GTTT'AAAC Cuts at: 3458 3458 Size: 6154 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2178 2210 4223 4255 5736 5768 2178 Size: 32 2013 32 1481 32 2564 Fragments arranged by size: 2564 2013 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2120 2830 1613 Size: 507 710 4937 Fragments arranged by size: 4937 710 507 PsiI TTA'TAA Cuts at: 3724 3724 Size: 6154 PspOMI G'GGCC_C Cuts at: 2044 2057 2044 Size: 13 6141 PstI C_TGCA'G Cuts at: 830 2038 2557 3506 830 Size: 1208 519 949 3478 Fragments arranged by size: 3478 1208 949 519 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6154 SalI G'TCGA_C Cuts at: 1936 3496 1936 Size: 1560 4594 SbfI CC_TGCA'GG Cuts at: 3506 3506 Size: 6154 ScaI AGT'ACT Cuts at: 2581 4402 6114 2581 Size: 1821 1712 2621 Fragments arranged by size: 2621 1821 1712 SfcI C'TryA_G Cuts at: 826 1045 2034 2553 2712 3502 4637 5318 5509 Size: 219 989 519 159 790 1135 681 191 Cuts at: 5509 826 Size: 1471 Fragments arranged by size: 1471 1135 989 790 681 519 219 191 159 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6154 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6154 SmaI CCC'GGG Cuts at: 1872 3481 1872 Size: 1609 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4258 5159 5421 820 Size: 197 926 2315 901 262 1553 Fragments arranged by size: 2315 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3945 493 Size: 3452 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6154 SphI G_CATG'C Cuts at: 1132 3512 1132 Size: 2380 3774 SspI AAT'ATT Cuts at: 5 52 4078 5 Size: 47 4026 2081 Fragments arranged by size: 4026 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6154 StyI C'CwwG_G Cuts at: 513 1065 2170 2873 3116 3593 513 Size: 552 1105 703 243 477 3074 Fragments arranged by size: 3074 1105 703 552 477 243 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3069 4181 4198 4351 4536 4981 325 Size: 1255 1489 1112 17 153 185 445 1498 Fragments arranged by size: 1498 1489 1255 1112 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2579 4400 6112 Size: 274 80 33 51 1443 602 1821 1712 Cuts at: 6112 96 Size: 138 Fragments arranged by size: 1821 1712 1443 602 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2023 2055 3049 3081 6114 6146 2023 Size: 32 994 32 3033 32 2031 Fragments arranged by size: 3033 2031 994 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6154 XbaI T'CTAG_A Cuts at: 2795 3011 3490 2795 Size: 216 479 5459 Fragments arranged by size: 5459 479 216 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6154 XmaI C'CCGG_G Cuts at: 1870 3479 1870 Size: 1609 4545 XmnI GAAnn'nnTTC Cuts at: 2746 4283 2746 Size: 1537 4617 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3962 276 Size: 53 83 186 936 2428 2468 Fragments arranged by size: 2468 2428 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI