################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccI AccII AcyI AflII AgeI AhdI Alw44I AocI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbsI BbvCI BcgI BciVI BfrI BglII BlnI BpmI BpuAI BsaHI BsaI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspMI BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FauI FseI FspI Hin1I HindIII KasI KpnI KspI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccIII 71 TCCGGA T^CCGGA AclI 1387 AACGTT AA^CGTT AcsI 1207 AAATTT [GA]^AATT[CT] AfaI 268 GTAC GT^AC AflIII 215 ACATGT A^C[GA][CT]GT AlwNI 1636 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 723 AGCGCT AGC^GCT ApoI 1207 AAATTT [GA]^AATT[CT] AseI 655 ATTAAT AT^TAAT AsnI 655 ATTAAT AT^TAAT BalI 479 TGGCCA TGG^CCA BanII 191 GAGCTC G[GA]GC[CT]^C BbrPI 454 CACGTG CAC^GTG BclI 374 TGATCA T^GATCA BfaI 1543 CTAG C^TAG BlpI 387 GCTGAGC GC^T[ACGT]AGC Bpu1102I 387 GCTGAGC GC^T[ACGT]AGC BsaAI 454 CACGTG [CT]AC^GT[GA] BsaWI 71 TCCGGA [AT]^CCGG[AT] BseAI 71 TCCGGA T^CCGGA BsiEI 408 CGACCG CG[GA][CT]^CG BspEI 71 TCCGGA T^CCGGA BspHI 1050 TCATGA T^CATGA BspLU11I 215 ACATGT A^CATGT BsrDI 569 CATTGC GCAATG(2/0) BsrFI 518 ACCGGC [GA]^CCGG[CT] BssSI 194 CTCGTG CACGAG(-5/-1) BstYI 68 AGATCC [GA]^GATC[CT] CelII 387 GCTGAGC GC^T[ACGT]AGC Cfr10I 518 ACCGGC [GA]^CCGG[CT] DraII 1315 AGGACCC [GA]G^G[ACGT]CC[CT] Eco47III 723 AGCGCT AGC^GCT EcoO109I 1315 AGGACCC [GA]G^G[ACGT]CC[CT] EcoT22I 500 ATGCAT ATGCA^T FbaI 374 TGATCA T^GATCA HaeII 723 AGCGCT [GA]GCGC^[CT] HgaI 1412 GACGC GACGC(5/10) MaeI 1543 CTAG C^TAG MflI 68 AGATCC [GA]^GATC[CT] MluNI 479 TGGCCA TGG^CCA MroI 71 TCCGGA T^CCGGA MscI 479 TGGCCA TGG^CCA MslI 553 CACTTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NsiI 500 ATGCAT ATGCA^T PleI 225 GACTC GAGTC(4/5) PmaCI 454 CACGTG CAC^GTG PmlI 454 CACGTG CAC^GTG PpuMI 1315 AGGACCC [GA]G^G[AT]CC[CT] PshAI 936 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 655 ATTAAT AT^TAAT Psp1406I 1387 AACGTT AA^CGTT RcaI 1050 TCATGA T^CATGA RsaI 268 GTAC GT^AC SacI 191 GAGCTC GAGCT^C SphI 630 GCATGC GCATG^C XbaI 1542 TCTAGA T^CTAGA XhoII 68 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 58 GATCC GGATC(4/5) 69 GATCC AvaII 516 GGACC G^G[AT]CC 1316 GGACC BcnI 237 CCGGG CC^[GC]GG 1581 CCGGG BglI 346 GCCAACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 481 GCCAAAGGGGC BsaBI 75 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1564 GATGCAAATC BseRI 1022 CTCCTC GAGGAG(10/8) 64 GAGGAG BsmAI 1363 GAGAC GTCTC(1/5) 403 GTCTC BstNI 681 CCTGG CC^[AT]GG 1508 CCAGG DsaI 582 CCATGG C^C[GA][CT]GG 945 CCATGG EarI 131 CTCTTC CTCTTC(1/4) 329 CTCTTC EcoRII 681 CCTGG ^CC[AT]GG 1508 CCAGG HincII 763 GTTAAC GT[CT]^[GA]AC 895 GTTAAC HindII 763 GTTAAC GT[CT]^[GA]AC 895 GTTAAC HpaI 763 GTTAAC GTT^AAC 895 GTTAAC Ksp632I 131 CTCTTC CTCTTC(1/4) 329 CTCTTC MamI 75 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1564 GATGCAAATC MvaI 681 CCTGG CC^[AT]GG 1508 CCAGG NciI 237 CCGGG CC^[GC]GG 1581 CCGGG NcoI 582 CCATGG C^CATGG 945 CCATGG NlaIV 1316 GGACCC GG[ACGT]^[ACGT]CC 1381 GGGGCC TfiI 112 GATTC G^A[AT]TC 1106 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AluI AspHI BbvI BmyI BsaJI BsgI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MwoI NdeII NlaIII NspI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI Tru9I TseI Tsp45I Tsp509I TspRI