(Circular) (Six-base) MAPSORT of: pFN21AE5677.seq Check: 865 from: 1 to: 6442 pFN21AE5677 6442 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4252 278 Size: 53 83 186 936 2716 2468 Fragments arranged by size: 2716 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3785 1937 Size: 1848 4594 Acc65I G'GTAC_C Cuts at: 3763 3763 Size: 6442 AclI AA'CG_TT Cuts at: 1563 3393 4569 4942 1563 Size: 1830 1176 373 3063 Fragments arranged by size: 3063 1830 1176 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2038 2118 3542 4505 5889 6157 1632 Size: 406 80 1424 963 1384 268 1917 Fragments arranged by size: 1917 1424 1384 963 406 268 80 AfeI AGC'GCT Cuts at: 2730 2730 Size: 6442 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6245 AflIII A'CryG_T Cuts at: 1203 2220 5341 6318 1203 Size: 1017 3121 977 1327 Fragments arranged by size: 3121 1327 1017 977 AgeI A'CCGG_T Cuts at: 5273 5273 Size: 6442 AhdI GACnn_n'nnGTC Cuts at: 5171 5171 Size: 6442 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6442 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2450 2856 3443 1184 Size: 1266 406 587 4183 Fragments arranged by size: 4183 1266 587 406 AlwNI CAG_nnn'CTG Cuts at: 3646 4185 5757 3646 Size: 539 1572 4331 Fragments arranged by size: 4331 1572 539 ApaLI G'TGCA_C Cuts at: 4499 5655 4499 Size: 1156 5286 ApoI r'AATT_y Cuts at: 1493 3212 3751 3977 4129 6238 1493 Size: 1719 539 226 152 2109 1697 Fragments arranged by size: 2109 1719 1697 539 226 152 AseI AT'TA_AT Cuts at: 160 1032 2661 4996 160 Size: 872 1629 2335 1606 Fragments arranged by size: 2335 1629 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6442 AvaI C'yCGr_G Cuts at: 1870 1943 3767 1870 Size: 73 1824 4545 Fragments arranged by size: 4545 1824 73 BamHI G'GATC_C Cuts at: 3772 4139 3772 Size: 367 6075 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3763 5218 618 Size: 325 217 627 1976 1455 1842 Fragments arranged by size: 1976 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2200 3761 1391 Size: 809 1561 4072 Fragments arranged by size: 4072 1561 809 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5471 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4633 4667 1903 Size: 34 2696 34 3678 Fragments arranged by size: 3678 2696 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4334 5544 4334 Size: 1210 5232 BclI T'GATC_A Cuts at: 1548 2379 1548 Size: 831 5611 BfrBI ATG'CAT Cuts at: 2507 2507 Size: 6442 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2357 2492 5053 Size: 107 122 71 631 605 685 135 2561 Cuts at: 5053 136 Size: 1525 Fragments arranged by size: 2561 1525 685 631 605 135 122 107 71 BglII A'GATC_T Cuts at: 6437 6437 Size: 6442 BlpI GC'TnA_GC Cuts at: 2393 3859 2393 Size: 1466 4976 Bme1580I G_kGCm'C Cuts at: 1790 3636 4503 5659 1790 Size: 1846 867 1156 2573 Fragments arranged by size: 2573 1846 1156 867 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6442 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2548 3421 4173 Size: 416 53 83 399 24 1131 873 752 Cuts at: 4173 5131 442 Size: 958 1753 Fragments arranged by size: 1753 1131 958 873 752 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6442 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5102 1239 Size: 3863 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4567 5432 5730 4567 Size: 865 298 5279 Fragments arranged by size: 5279 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5105 882 Size: 617 3606 2219 Fragments arranged by size: 3606 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2461 4233 493 Size: 711 1257 1772 2702 Fragments arranged by size: 2702 1772 1257 711 BsaBI GATnn'nnATC Cuts at: 2084 3573 6436 2084 Size: 1489 2863 2090 Fragments arranged by size: 2863 2090 1489 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4249 4631 5266 275 Size: 53 83 186 936 2716 382 635 1451 Fragments arranged by size: 2716 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2076 4875 5273 5547 5694 6105 2076 Size: 2799 398 274 147 411 2413 Fragments arranged by size: 2799 2413 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2187 2217 3240 3270 1178 Size: 30 165 30 784 30 1023 30 4350 Fragments arranged by size: 4350 1023 784 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2084 3018 1186 Size: 898 934 4610 Fragments arranged by size: 4610 934 898 BseYI C'CCAG_C Cuts at: 5645 6141 5645 Size: 496 5946 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2222 2391 2519 2918 1163 Size: 573 486 169 128 399 4687 Fragments arranged by size: 4687 573 486 399 169 128 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2416 3892 4653 5681 1540 Size: 275 186 415 1476 761 1028 2301 Fragments arranged by size: 2301 1476 1028 761 415 275 186 BsiHKAI G_wGCw'C Cuts at: 2016 2200 2533 4503 4588 5659 2016 Size: 184 333 1970 85 1071 2799 Fragments arranged by size: 2799 1970 1071 333 184 85 BsmI GAATG_Cn' Cuts at: 3953 4046 3953 Size: 93 6349 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2200 2533 3636 3761 4503 4588 Size: 399 226 184 333 1103 125 742 85 Cuts at: 4588 5659 1391 Size: 1071 2174 Fragments arranged by size: 2174 1103 1071 742 399 333 226 184 125 85 BspEI T'CCGG_A Cuts at: 2076 6105 2076 Size: 4029 2413 BspHI T'CATG_A Cuts at: 3055 4331 6061 3055 Size: 1276 1730 3436 Fragments arranged by size: 3436 1730 1276 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3797 844 Size: 2953 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4329 1394 Size: 429 2506 3507 Fragments arranged by size: 3507 2506 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2573 4937 5111 65 Size: 1183 1325 2364 174 1396 Fragments arranged by size: 2364 1396 1325 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2523 5086 5273 1700 Size: 110 713 2563 187 2869 Fragments arranged by size: 2869 2563 713 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6442 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6442 BssSI C'ACGA_G Cuts at: 2199 4502 5514 2199 Size: 2303 1012 3127 Fragments arranged by size: 3127 2303 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6442 BstBI TT'CG_AA Cuts at: 5246 5246 Size: 6442 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6442 BstYI r'GATC_y Cuts at: 1972 2073 3772 4139 4524 4541 5993 6079 6091 Size: 101 1699 367 385 17 1452 86 12 Cuts at: 6091 6437 1972 Size: 346 1977 Fragments arranged by size: 1977 1699 1452 385 367 346 101 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2587 2950 513 Size: 552 939 583 363 4005 Fragments arranged by size: 4005 939 583 552 363 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2722 487 Size: 683 56 1496 4207 Fragments arranged by size: 4207 1496 683 56 BtsI GCAGTG_nn' Cuts at: 2016 2234 2404 2930 3647 3958 4752 4772 2016 Size: 218 170 526 717 311 794 20 3686 Fragments arranged by size: 3686 794 717 526 311 218 170 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6442 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6407 DraI TTT'AAA Cuts at: 3746 3746 Size: 6442 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5449 809 Size: 507 4133 1802 Fragments arranged by size: 4133 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2145 2455 2484 Size: 54 1425 50 275 18 315 310 29 Cuts at: 2484 3889 4778 6407 8 Size: 1405 889 1629 43 Fragments arranged by size: 1629 1425 1405 889 315 310 275 54 50 43 29 18 EagI C'GGCC_G Cuts at: 1537 1812 3889 1537 Size: 275 2077 4090 Fragments arranged by size: 4090 2077 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2142 2340 4372 1353 Size: 789 198 2032 3423 Fragments arranged by size: 3423 2032 789 198 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5029 5403 5549 387 Size: 1064 138 3440 374 146 1280 Fragments arranged by size: 3440 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2198 2198 Size: 6442 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2038 2118 3542 4505 5102 5889 6157 Size: 393 406 80 1424 963 597 787 268 Cuts at: 6157 1239 Size: 1524 Fragments arranged by size: 1524 1424 963 787 597 406 393 268 80 EcoO109I rG'GnC_Cy Cuts at: 3321 3321 Size: 6442 EcoRI G'AATT_C Cuts at: 3751 4129 3751 Size: 378 6064 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3487 3487 Size: 6442 FspI TGC'GCA Cuts at: 4948 4948 Size: 6442 HaeII r_GCGC'y Cuts at: 1678 2732 5589 1678 Size: 1054 2857 2531 Fragments arranged by size: 2857 2531 1054 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5083 5115 5157 5189 1373 Size: 32 135 32 3511 32 42 32 2626 Fragments arranged by size: 3511 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2770 2902 3786 4032 669 Size: 988 281 832 132 884 246 3079 Fragments arranged by size: 3079 988 884 832 281 246 132 HindIII A'AGCT_T Cuts at: 748 748 Size: 6442 HpaI GTT'AAC Cuts at: 2770 2902 4032 2770 Size: 132 1130 5180 Fragments arranged by size: 5180 1130 132 KpnI G_GTAC'C Cuts at: 3767 3767 Size: 6442 MfeI C'AATT_G Cuts at: 4041 4041 Size: 6442 MluI A'CGCG_T Cuts at: 6318 6318 Size: 6442 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2436 5556 5740 1305 Size: 1131 3120 184 2007 Fragments arranged by size: 3120 2007 1131 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2486 10 Size: 54 1425 343 654 3966 Fragments arranged by size: 3966 1425 654 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2562 4400 4759 4918 518 Size: 648 843 553 1838 359 159 2042 Fragments arranged by size: 2042 1838 843 648 553 359 159 MspA1I CmG'CkG Cuts at: 1606 2058 2297 2945 3087 3285 3341 3440 3858 Size: 452 239 648 142 198 56 99 418 Cuts at: 3858 4535 5683 5928 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 648 452 418 245 239 198 142 99 56 NcoI C'CATG_G Cuts at: 513 1065 2004 2587 2950 513 Size: 552 939 583 363 4005 Fragments arranged by size: 4005 939 583 552 363 NdeI CA'TA_TG Cuts at: 387 387 Size: 6442 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6442 NotI GC'GGCC_GC Cuts at: 3889 3889 Size: 6442 NsiI A_TGCA'T Cuts at: 2509 2509 Size: 6442 NspI r_CATG'y Cuts at: 1132 1229 2224 2639 3124 3800 5345 1132 Size: 97 995 415 485 676 1545 2229 Fragments arranged by size: 2229 1545 995 676 485 415 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6442 PciI A'CATG_T Cuts at: 2220 5341 2220 Size: 3121 3321 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6442 PfoI T'CCnGG_A Cuts at: 3585 3585 Size: 6442 PmeI GTTT'AAAC Cuts at: 3746 3746 Size: 6442 PmlI CAC'GTG Cuts at: 2461 2461 Size: 6442 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4511 4543 6024 6056 4511 Size: 32 1481 32 4897 Fragments arranged by size: 4897 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3321 3321 Size: 6442 PshAI GACnn'nnGTC Cuts at: 1613 2945 1613 Size: 1332 5110 PsiI TTA'TAA Cuts at: 4012 4012 Size: 6442 PstI C_TGCA'G Cuts at: 830 2644 3170 3247 3794 830 Size: 1814 526 77 547 3478 Fragments arranged by size: 3478 1814 547 526 77 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6442 PvuII CAG'CTG Cuts at: 2297 2945 3341 2297 Size: 648 396 5398 Fragments arranged by size: 5398 648 396 SacI G_AGCT'C Cuts at: 2200 2200 Size: 6442 SalI G'TCGA_C Cuts at: 1936 3784 1936 Size: 1848 4594 SbfI CC_TGCA'GG Cuts at: 3794 3794 Size: 6442 ScaI AGT'ACT Cuts at: 4690 6402 4690 Size: 1712 4730 SfcI C'TryA_G Cuts at: 826 1045 2640 3166 3243 3790 4925 5606 5797 Size: 219 1595 526 77 547 1135 681 191 Cuts at: 5797 826 Size: 1471 Fragments arranged by size: 1595 1471 1135 681 547 526 219 191 77 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6442 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6442 SmaI CCC'GGG Cuts at: 1872 3769 1872 Size: 1897 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4546 5447 5709 820 Size: 197 926 2603 901 262 1553 Fragments arranged by size: 2603 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4233 493 Size: 3740 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6442 SphI G_CATG'C Cuts at: 1132 2639 3800 1132 Size: 1507 1161 3774 Fragments arranged by size: 3774 1507 1161 SspI AAT'ATT Cuts at: 5 52 4366 5 Size: 47 4314 2081 Fragments arranged by size: 4314 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6442 StyI C'CwwG_G Cuts at: 513 1065 2004 2211 2587 2950 3881 513 Size: 552 939 207 376 363 931 3074 Fragments arranged by size: 3074 939 931 552 376 363 207 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2430 4469 4486 4639 4824 5269 325 Size: 1255 850 2039 17 153 185 445 1498 Fragments arranged by size: 2039 1498 1255 850 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2272 4688 6400 Size: 274 80 33 51 1443 295 2416 1712 Cuts at: 6400 96 Size: 138 Fragments arranged by size: 2416 1712 1443 295 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2207 2239 3240 3272 6402 6434 2207 Size: 32 1001 32 3130 32 2215 Fragments arranged by size: 3130 2215 1001 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6442 XbaI T'CTAG_A Cuts at: 3547 3778 3547 Size: 231 6211 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6442 XmaI C'CCGG_G Cuts at: 1870 3767 1870 Size: 1897 4545 XmnI GAAnn'nnTTC Cuts at: 4571 4571 Size: 6442 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4250 276 Size: 53 83 186 936 2716 2468 Fragments arranged by size: 2716 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI