(Linear) (Six-base) MAPSORT of: KIEE5677.seq Check: 4910 from: 1 to: 1737 KIEE5677.orf With 182 enzymes: SgfI * December 5, 2014 13:06 .. AclI AA'CG_TT Cuts at: 0 1388 1737 Size: 1388 349 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 33 113 1537 1737 Size: 33 80 1424 200 Fragments arranged by size: 1424 200 80 33 AfeI AGC'GCT Cuts at: 0 725 1737 Size: 725 1012 AflIII A'CryG_T Cuts at: 0 215 1737 Size: 215 1522 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 445 851 1438 1737 Size: 445 406 587 299 Fragments arranged by size: 587 445 406 299 AlwNI CAG_nnn'CTG Cuts at: 0 1641 1737 Size: 1641 96 ApoI r'AATT_y Cuts at: 0 1207 1737 Size: 1207 530 AseI AT'TA_AT Cuts at: 0 656 1737 Size: 656 1081 BanII G_rGCy'C Cuts at: 0 195 1737 Size: 195 1542 BclI T'GATC_A Cuts at: 0 374 1737 Size: 374 1363 BfrBI ATG'CAT Cuts at: 0 502 1737 Size: 502 1235 BglI GCCn_nnn'nGGC Cuts at: 0 352 487 1737 Size: 352 135 1250 Fragments arranged by size: 1250 352 135 BlpI GC'TnA_GC Cuts at: 0 388 1737 Size: 388 1349 Bme1580I G_kGCm'C Cuts at: 0 1631 1737 Size: 1631 106 BmrI ACTGGGnnnn_n' Cuts at: 0 543 1416 1737 Size: 543 873 321 Fragments arranged by size: 873 543 321 BsaAI yAC'GTr Cuts at: 0 456 1737 Size: 456 1281 BsaBI GATnn'nnATC Cuts at: 0 79 1568 1737 Size: 79 1489 169 Fragments arranged by size: 1489 169 79 BsaWI w'CCGG_w Cuts at: 0 71 1737 Size: 71 1666 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 182 212 1235 1265 1737 Size: 182 30 1023 30 472 Fragments arranged by size: 1023 472 182 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 79 1013 1737 Size: 79 934 724 Fragments arranged by size: 934 724 79 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 217 386 514 913 1737 Size: 217 169 128 399 824 Fragments arranged by size: 824 399 217 169 128 BsiEI CG_ry'CG Cuts at: 0 411 1737 Size: 411 1326 BsiHKAI G_wGCw'C Cuts at: 0 11 195 528 1737 Size: 11 184 333 1209 Fragments arranged by size: 1209 333 184 11 Bsp1286I G_dGCh'C Cuts at: 0 11 195 528 1631 1737 Size: 11 184 333 1103 106 Fragments arranged by size: 1103 333 184 106 11 BspEI T'CCGG_A Cuts at: 0 71 1737 Size: 71 1666 BspHI T'CATG_A Cuts at: 0 1050 1737 Size: 1050 687 BsrDI GCAATG_nn' Cuts at: 0 568 1737 Size: 568 1169 BsrFI r'CCGG_y Cuts at: 0 518 1737 Size: 518 1219 BssSI C'ACGA_G Cuts at: 0 194 1737 Size: 194 1543 BstYI r'GATC_y Cuts at: 0 68 1737 Size: 68 1669 BtgI C'CryG_G Cuts at: 0 582 945 1737 Size: 582 363 792 Fragments arranged by size: 792 582 363 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 717 1737 Size: 717 1020 BtsI GCAGTG_nn' Cuts at: 0 11 229 399 925 1642 1737 Size: 11 218 170 526 717 95 Fragments arranged by size: 717 526 218 170 95 11 EaeI y'GGCC_r Cuts at: 0 140 450 479 1737 Size: 140 310 29 1258 Fragments arranged by size: 1258 310 140 29 EarI CTCTTCn'nnn_ Cuts at: 0 137 335 1737 Size: 137 198 1402 Fragments arranged by size: 1402 198 137 EcoICRI GAG'CTC Cuts at: 0 193 1737 Size: 193 1544 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 33 113 1537 1737 Size: 33 80 1424 200 Fragments arranged by size: 1424 200 80 33 EcoO109I rG'GnC_Cy Cuts at: 0 1316 1737 Size: 1316 421 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1482 1737 Size: 1482 255 HaeII r_GCGC'y Cuts at: 0 727 1737 Size: 727 1010 HincII GTy'rAC Cuts at: 0 765 897 1737 Size: 765 132 840 Fragments arranged by size: 840 765 132 HpaI GTT'AAC Cuts at: 0 765 897 1737 Size: 765 132 840 Fragments arranged by size: 840 765 132 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 431 1737 Size: 431 1306 MscI TGG'CCA Cuts at: 0 481 1737 Size: 481 1256 MslI CAynn'nnrTG Cuts at: 0 557 1737 Size: 557 1180 MspA1I CmG'CkG Cuts at: 0 53 292 940 1082 1280 1336 1435 1737 Size: 53 239 648 142 198 56 99 302 Fragments arranged by size: 648 302 239 198 142 99 56 53 NcoI C'CATG_G Cuts at: 0 582 945 1737 Size: 582 363 792 Fragments arranged by size: 792 582 363 NsiI A_TGCA'T Cuts at: 0 504 1737 Size: 504 1233 NspI r_CATG'y Cuts at: 0 219 634 1119 1737 Size: 219 415 485 618 Fragments arranged by size: 618 485 415 219 PciI A'CATG_T Cuts at: 0 215 1737 Size: 215 1522 PfoI T'CCnGG_A Cuts at: 0 1580 1737 Size: 1580 157 PmlI CAC'GTG Cuts at: 0 456 1737 Size: 456 1281 PpuMI rG'GwC_Cy Cuts at: 0 1316 1737 Size: 1316 421 PshAI GACnn'nnGTC Cuts at: 0 940 1737 Size: 940 797 PstI C_TGCA'G Cuts at: 0 639 1165 1242 1737 Size: 639 526 77 495 Fragments arranged by size: 639 526 495 77 PvuII CAG'CTG Cuts at: 0 292 940 1336 1737 Size: 292 648 396 401 Fragments arranged by size: 648 401 396 292 SacI G_AGCT'C Cuts at: 0 195 1737 Size: 195 1542 SfcI C'TryA_G Cuts at: 0 635 1161 1238 1737 Size: 635 526 77 499 Fragments arranged by size: 635 526 499 77 SphI G_CATG'C Cuts at: 0 634 1737 Size: 634 1103 StyI C'CwwG_G Cuts at: 0 206 582 945 1737 Size: 206 376 363 792 Fragments arranged by size: 792 376 363 206 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 425 1737 Size: 425 1312 TatI w'GTAC_w Cuts at: 0 267 1737 Size: 267 1470 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 202 234 1235 1267 1737 Size: 202 32 1001 32 470 Fragments arranged by size: 1001 470 202 32 32 XbaI T'CTAG_A Cuts at: 0 1542 1737 Size: 1542 195 Enzymes that do cut: AclI AcuI AfeI AflIII AlfI AlwNI ApoI AseI BanII BclI BfrBI BglI BlpI Bme1580I BmrI BsaAI BsaBI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BsrDI BsrFI BssSI BstYI BtgI BtgZI BtsI EaeI EarI EcoICRI Eco57MI EcoO109I FalI HaeII HincII HpaI MmeI MscI MslI MspA1I NcoI NsiI NspI PciI PfoI PmlI PpuMI PshAI PstI PvuII SacI SfcI SphI StyI TaqII TatI TstI XbaI Enzymes that do not cut: AarI AatII AccI Acc65I AflII AgeI AhdI AleI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BglII BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaHI BseYI BsiWI BsmI BsmBI BspMI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HindIII KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PflMI PmeI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XcmI XhoI XmaI XmnI ZraI