################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AclI AgeI AhdI AlwI AocI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BciVI BclI BfaI BlnI BlpI Bpu1102I BsaAI BsaI BsgI BsiEI BsiWI BsoBI Bsp1407I BspDI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI HindIII HpaI KasI KpnI KspI MaeI MfeI MluI MluNI MscI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 1531 GTCTAC GT^[AC][GT]AC AccIII 1106 TCCGGA T^CCGGA AflII 1654 CTTAAG C^TTAAG AflIII 206 ACGTGT A^C[GA][CT]GT Alw44I 1246 GTGCAC G^TGCAC AlwNI 1202 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1246 GTGCAC G^TGCAC Asp700I 1098 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 414 GGTGCC G^G[CT][GA]CC BanII 564 GGGCTC G[GA]GC[CT]^C BbvCI 1476 GCTGAGG CCTCAGC(-5/-2) BfrI 1654 CTTAAG C^TTAAG BglI 1282 GCCCCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1049 AGATCT A^GATCT BsaBI 1044 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1106 TCCGGA [AT]^CCGG[AT] BseAI 1106 TCCGGA T^CCGGA BspEI 1106 TCCGGA T^CCGGA BspHI 185 TCATGA T^CATGA BsrBI 1093 GAGCGG CCGCTC(-3/-3) BsrDI 1178 GCAATG GCAATG(2/0) BsrFI 328 GCCGGC [GA]^CCGG[CT] BstXI 314 CCAGCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1049 AGATCT [GA]^GATC[CT] Cfr10I 328 GCCGGC [GA]^CCGG[CT] EaeI 1198 CGGCCA [CT]^GGCC[GA] EcoRV 487 GATATC GAT^ATC FauI 795 GCGGG CCCGC(4/6) HincII 1561 GTCAAC GT[CT]^[GA]AC HindII 1561 GTCAAC GT[CT]^[GA]AC MamI 1044 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1049 AGATCT [GA]^GATC[CT] MroI 1106 TCCGGA T^CCGGA MseI 1655 TTAA T^TAA NaeI 328 GCCGGC GCC^GGC NgoMIV 328 GCCGGC G^CCGGC PvuII 745 CAGCTG CAG^CTG RcaI 185 TCATGA T^CATGA SapI 477 GCTCTTC GCTCTTC(1/4) SbfI 304 CCTGCAGG CCTGCA^GG Sse8387I 304 CCTGCAGG CCTGCA^GG StuI 669 AGGCCT AGG^CCT TfiI 1553 GAATC G^A[AT]TC Tru9I 1655 TTAA T^TAA XhoII 1049 AGATCT [GA]^GATC[CT] XmnI 1098 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 1084 CGCG CG^CG 1443 CGCG AcsI 190 AAATTC [GA]^AATT[CT] 1640 AAATTC AcyI 1144 GGCGTC G[GA]^CG[CT]C 1218 GACGCC Aor51HI 428 AGCGCT AGC^GCT 1265 AGCGCT ApoI 190 AAATTC [GA]^AATT[CT] 1640 AAATTC BbsI 1455 GAAGAC GAAGAC(2/6) 715 GTCTTC BcgI 1279 GCAGCCCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1595 GCAACCACGTCG BcnI 504 CCCGG CC^[GC]GG 773 CCCGG BpuAI 1455 GAAGAC GAAGAC(2/6) 715 GTCTTC BsaHI 1144 GGCGTC G[GA]^CG[CT]C 1218 GACGCC BstUI 1084 CGCG CG^CG 1443 CGCG DsaI 132 CCATGG C^C[GA][CT]GG 605 CCATGG Eco47III 428 AGCGCT AGC^GCT 1265 AGCGCT Hin1I 1144 GGCGTC G[GA]^CG[CT]C 1218 GACGCC MslI 360 CACTGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 801 CATTGACGTG MspA1I 745 CAGCTG C[AC]G^C[GT]G 793 CAGCGG MvnI 1084 CGCG CG^CG 1443 CGCG NciI 504 CCCGG CC^[GC]GG 773 CCCGG NcoI 132 CCATGG C^CATGG 605 CCATGG PflMI 132 CCATGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 655 CCAGCAGGTGG SfaNI 474 GATGC GCATC(5/9) 1080 GATGC TaqI 472 TCGA T^CGA 719 TCGA Tsp509I 191 AATT ^AATT 1641 AATT TthHB8I 472 TCGA T^CGA 719 TCGA Van91I 132 CCATGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 655 CCAGCAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AfaI AluI AspHI AvaII BbvI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfcI SmlI StyI TseI Tsp45I TspRI