(Linear) (Six-base) MAPSORT of: KIEE2519.seq Check: 8714 from: 1 to: 1665 KIEE2519.orf With 182 enzymes: SgfI * April 4, 2014 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 649 1665 Size: 649 1016 AccI GT'mk_AC Cuts at: 0 1532 1665 Size: 1532 133 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1155 1342 1378 1489 1532 1665 Size: 1155 187 36 111 43 133 Fragments arranged by size: 1155 187 133 111 43 36 AfeI AGC'GCT Cuts at: 0 430 1267 1665 Size: 430 837 398 Fragments arranged by size: 837 430 398 AflII C'TTAA_G Cuts at: 0 1654 1665 Size: 1654 11 AflIII A'CryG_T Cuts at: 0 206 1665 Size: 206 1459 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 800 1665 Size: 800 865 AlwNI CAG_nnn'CTG Cuts at: 0 1207 1665 Size: 1207 458 ApaLI G'TGCA_C Cuts at: 0 1246 1665 Size: 1246 419 ApoI r'AATT_y Cuts at: 0 190 1640 1665 Size: 190 1450 25 Fragments arranged by size: 1450 190 25 BanI G'GyrC_C Cuts at: 0 414 1665 Size: 414 1251 BanII G_rGCy'C Cuts at: 0 568 1665 Size: 568 1097 BbsI GAAGACnn'nnnn_ Cuts at: 0 708 1462 1665 Size: 708 754 203 Fragments arranged by size: 754 708 203 BbvCI CC'TCA_GC Cuts at: 0 1477 1665 Size: 1477 188 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1268 1302 1584 1618 1665 Size: 1268 34 282 34 47 Fragments arranged by size: 1268 282 47 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 1288 1665 Size: 1288 377 BglII A'GATC_T Cuts at: 0 1049 1665 Size: 1049 616 Bme1580I G_kGCm'C Cuts at: 0 1012 1126 1250 1665 Size: 1012 114 124 415 Fragments arranged by size: 1012 415 124 114 BmgBI CAC'GTC Cuts at: 0 807 1602 1665 Size: 807 795 63 Fragments arranged by size: 807 795 63 BmrI ACTGGGnnnn_n' Cuts at: 0 107 730 1665 Size: 107 623 935 Fragments arranged by size: 935 623 107 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 348 1665 Size: 348 1317 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 75 343 1116 1234 1239 1292 1665 Size: 75 268 773 118 5 53 373 Fragments arranged by size: 773 373 268 118 75 53 5 Bpu10I CC'TnA_GC Cuts at: 0 1477 1665 Size: 1477 188 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 57 223 1665 Size: 57 166 1442 Fragments arranged by size: 1442 166 57 BsaBI GATnn'nnATC Cuts at: 0 1048 1665 Size: 1048 617 BsaHI Gr'CG_yC Cuts at: 0 1145 1219 1665 Size: 1145 74 446 Fragments arranged by size: 1145 446 74 BsaWI w'CCGG_w Cuts at: 0 1106 1665 Size: 1106 559 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 865 1363 1444 1665 Size: 865 498 81 221 Fragments arranged by size: 865 498 221 81 BseYI C'CCAG_C Cuts at: 0 313 532 654 1297 1665 Size: 313 219 122 643 368 Fragments arranged by size: 643 368 313 219 122 BsiHKAI G_wGCw'C Cuts at: 0 845 934 1250 1665 Size: 845 89 316 415 Fragments arranged by size: 845 415 316 89 BsmI GAATG_Cn' Cuts at: 0 404 768 1488 1665 Size: 404 364 720 177 Fragments arranged by size: 720 404 364 177 BsmBI CGTCTCn'nnnn_ Cuts at: 0 142 1177 1210 1665 Size: 142 1035 33 455 Fragments arranged by size: 1035 455 142 33 Bsp1286I G_dGCh'C Cuts at: 0 568 845 934 1012 1126 1250 1665 Size: 568 277 89 78 114 124 415 Fragments arranged by size: 568 415 277 124 114 89 78 BspEI T'CCGG_A Cuts at: 0 1106 1665 Size: 1106 559 BspHI T'CATG_A Cuts at: 0 185 1665 Size: 185 1480 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 649 986 1339 1665 Size: 649 337 353 326 Fragments arranged by size: 649 353 337 326 BsrBI CCG'CTC Cuts at: 0 1095 1665 Size: 1095 570 BsrDI GCAATG_nn' Cuts at: 0 1185 1665 Size: 1185 480 BsrFI r'CCGG_y Cuts at: 0 328 1665 Size: 328 1337 BstXI CCAn_nnnn'nTGG Cuts at: 0 321 1665 Size: 321 1344 BstYI r'GATC_y Cuts at: 0 1049 1665 Size: 1049 616 BtgI C'CryG_G Cuts at: 0 132 605 1665 Size: 132 473 1060 Fragments arranged by size: 1060 473 132 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1100 1665 Size: 1100 565 BtsI GCAGTG_nn' Cuts at: 0 782 1665 Size: 782 883 EaeI y'GGCC_r Cuts at: 0 1198 1665 Size: 1198 467 EarI CTCTTCn'nnn_ Cuts at: 0 484 890 1508 1665 Size: 484 406 618 157 Fragments arranged by size: 618 484 406 157 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 75 343 1116 1155 1234 1239 1292 1342 Size: 75 268 773 39 79 5 53 50 Cuts at: 1342 1378 1489 1532 1665 Size: 36 111 43 133 Fragments arranged by size: 773 268 133 111 79 75 53 50 43 39 36 5 EcoO109I rG'GnC_Cy Cuts at: 0 64 269 310 732 1294 1665 Size: 64 205 41 422 562 371 Fragments arranged by size: 562 422 371 205 64 41 EcoRV GAT'ATC Cuts at: 0 489 1665 Size: 489 1176 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1408 1665 Size: 1408 257 HaeII r_GCGC'y Cuts at: 0 432 1020 1269 1665 Size: 432 588 249 396 Fragments arranged by size: 588 432 396 249 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 193 225 364 396 928 960 1665 Size: 193 32 139 32 532 32 705 Fragments arranged by size: 705 532 193 139 32 32 32 HincII GTy'rAC Cuts at: 0 1563 1665 Size: 1563 102 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 710 1331 1665 Size: 710 621 334 Fragments arranged by size: 710 621 334 MslI CAynn'nnrTG Cuts at: 0 364 805 1665 Size: 364 441 860 Fragments arranged by size: 860 441 364 MspA1I CmG'CkG Cuts at: 0 747 795 1665 Size: 747 48 870 Fragments arranged by size: 870 747 48 NaeI GCC'GGC Cuts at: 0 330 1665 Size: 330 1335 NcoI C'CATG_G Cuts at: 0 132 605 1665 Size: 132 473 1060 Fragments arranged by size: 1060 473 132 NgoMIV G'CCGG_C Cuts at: 0 328 1665 Size: 328 1337 PasI CC'CwG_GG Cuts at: 0 561 1665 Size: 561 1104 PflMI CCAn_nnn'nTGG Cuts at: 0 138 661 1665 Size: 138 523 1004 Fragments arranged by size: 1004 523 138 PpuMI rG'GwC_Cy Cuts at: 0 64 732 1294 1665 Size: 64 668 562 371 Fragments arranged by size: 668 562 371 64 PstI C_TGCA'G Cuts at: 0 309 983 1265 1665 Size: 309 674 282 400 Fragments arranged by size: 674 400 309 282 PvuII CAG'CTG Cuts at: 0 747 1665 Size: 747 918 SanDI GG'GwC_CC Cuts at: 0 64 1665 Size: 64 1601 SapI GCTCTTCn'nnn_ Cuts at: 0 484 1665 Size: 484 1181 SbfI CC_TGCA'GG Cuts at: 0 309 1665 Size: 309 1356 SfcI C'TryA_G Cuts at: 0 305 979 1261 1665 Size: 305 674 282 404 Fragments arranged by size: 674 404 305 282 SmlI C'TyrA_G Cuts at: 0 36 238 1654 1665 Size: 36 202 1416 11 Fragments arranged by size: 1416 202 36 11 StuI AGG'CCT Cuts at: 0 671 1665 Size: 671 994 StyI C'CwwG_G Cuts at: 0 68 114 132 605 902 1379 1665 Size: 68 46 18 473 297 477 286 Fragments arranged by size: 477 473 297 286 68 46 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 618 1665 Size: 618 1047 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 754 786 1665 Size: 754 32 879 Fragments arranged by size: 879 754 32 XmnI GAAnn'nnTTC Cuts at: 0 1102 1665 Size: 1102 563 Enzymes that do cut: AarI AccI AcuI AfeI AflII AflIII AlfI AlwNI ApaLI ApoI BanI BanII BbsI BbvCI BcgI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BstXI BstYI BtgI BtgZI BtsI EaeI EarI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII MmeI MslI MspA1I NaeI NcoI NgoMIV PasI PflMI PpuMI PstI PvuII SanDI SapI SbfI SfcI SmlI StuI StyI TaqII TstI XmnI Enzymes that do not cut: AatII Acc65I AclI AgeI AhdI AleI AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BciVI BclI BfrBI BlpI BmtI BsaI BsaAI BsaXI BsgI BsiEI BsiWI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MscI NarI NdeI NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XcmI XhoI XmaI ZraI