################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII AccII AccIII AclI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI AspEI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BpmI BseAI BseRI BsgI BsiWI BsmBI BsmI Bsp1407I BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstUI Bsu36I Cfr10I CpoI DraII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoT22I FauI FbaI FseI FspI HgaI HindIII HpaI KspI MluI MroI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### Acc65I 1197 GGTACC G^GTACC AciI 1059 GCGG CCGC(-3/-1) AcyI 821 GGCGCC G[GA]^CG[CT]C AflIII 975 ACGTGT A^C[GA][CT]GT Alw44I 110 GTGCAC G^TGCAC ApaLI 110 GTGCAC G^TGCAC Asp718I 1197 GGTACC G^GTACC AspHI 110 GTGCAC G[AT]GC[AT]^C AvaI 446 CCCGAG C^[CT]CG[GA]G BbeI 821 GGCGCC GGCGC^C BbvI 477 GCAGC GCAGC(8/12) BlpI 1476 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1476 GCTAAGC GC^T[ACGT]AGC BsaAI 974 TACGTG [CT]AC^GT[GA] BsaBI 97 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 821 GGCGCC G[GA]^CG[CT]C BsaI 779 GAGACC GGTCTC(1/5) BsaWI 1718 ACCGGA [AT]^CCGG[AT] BsiEI 1716 CGACCG CG[GA][CT]^CG BsiHKAI 110 GTGCAC G[AT]GC[AT]^C BsmFI 1175 GGGAC GGGAC(10/14) BsoBI 446 CCCGAG C^[CT]CG[GA]G BspDI 286 ATCGAT AT^CGAT BsrDI 889 CATTGC GCAATG(2/0) Bst1107I 1314 GTATAC GTA^TAC BstEII 457 GGTAACC G^GT[ACGT]ACC BstPI 457 GGTAACC G^GT[ACGT]ACC BstXI 256 CCAACACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1314 GTATAC GTA^TAC CelII 1476 GCTAAGC GC^T[ACGT]AGC ClaI 286 ATCGAT AT^CGAT DraI 1263 TTTAAA TTT^AAA DraIII 612 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1177 GACACTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1595 CCATGG C^C[GA][CT]GG EcoO65I 457 GGTAACC G^GT[ACGT]ACC EcoRV 1676 GATATC GAT^ATC Fnu4HI 477 GCAGC GC^[ACGT]GC HaeII 821 GGCGCC [GA]GCGC^[CT] Hin1I 821 GGCGCC G[GA]^CG[CT]C HincII 676 GTCAAC GT[CT]^[GA]AC HindII 676 GTCAAC GT[CT]^[GA]AC ItaI 477 GCAGC GC^[ACGT]GC KasI 821 GGCGCC G^GCGCC KpnI 1197 GGTACC GGTAC^C MamI 97 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 574 CAATTG C^AATTG MspA1I 122 CAGCTG C[AC]G^C[GT]G MunI 574 CAATTG C^AATTG MwoI 409 GCTGAAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NarI 821 GGCGCC GG^CGCC NcoI 1595 CCATGG C^CATGG PvuII 122 CAGCTG CAG^CTG SapI 1533 GAAGAGC GCTCTTC(1/4) SfoI 821 GGCGCC GGC^GCC TaqI 287 TCGA T^CGA TseI 477 GCAGC G^C[AT]GC TthHB8I 287 TCGA T^CGA XbaI 1837 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 658 GTCTAC GT^[AC][GT]AC 1314 GTATAC AcsI 82 AAATTT [GA]^AATT[CT] 1498 AAATTT ApoI 82 AAATTT [GA]^AATT[CT] 1498 AAATTT BalI 265 TGGCCA TGG^CCA 1380 TGGCCA BanII 252 GAGCCC G[GA]GC[CT]^C 427 GAGCCC BcnI 152 CCCGG CC^[GC]GG 1319 CCCGG BglII 96 AGATCT A^GATCT 1432 AGATCT BsmAI 779 GAGAC GTCTC(1/5) 232 GTCTC BspHI 77 TCATGA T^CATGA 1118 TCATGA CfoI 717 GCGC GCG^C 822 GCGC EaeI 265 TGGCCA [CT]^GGCC[GA] 1380 TGGCCA HhaI 717 GCGC GCG^C 822 GCGC HinP1I 717 GCGC G^CGC 822 GCGC HphI 136 GGTGA GGTGA(8/7) 683 TCACC MaeII 201 ACGT A^CGT 975 ACGT MluNI 265 TGGCCA TGG^CCA 1380 TGGCCA MscI 265 TGGCCA TGG^CCA 1380 TGGCCA NciI 152 CCCGG CC^[GC]GG 1319 CCCGG RcaI 77 TCATGA T^CATGA 1118 TCATGA SfcI 538 CTACAG C^T[GA][CT]AG 660 CTACAG SmlI 1050 CTCAAG C^T[CT][GA]AG 1152 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AfaI AluI AlwI Asp700I AvaII BanI BbsI BfaI BmyI BpuAI BsaJI BsiYI BslI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I FokI HaeIII HapII HinfI HpaII Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I Tsp45I Tsp509I TspRI XhoII XmnI