(Circular) (Six-base) MAPSORT of: pFN21AE6484.seq Check: 7732 from: 1 to: 6568 pFN21AE6484 6568 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4378 278 Size: 53 83 186 936 2842 2468 Fragments arranged by size: 2842 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2664 3320 3911 1937 Size: 727 656 591 4594 Fragments arranged by size: 4594 727 656 591 Acc65I G'GTAC_C Cuts at: 3202 3889 3202 Size: 687 5881 AclI AA'CG_TT Cuts at: 1563 4695 5068 1563 Size: 3132 373 3063 Fragments arranged by size: 3132 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2436 3314 4631 6015 6283 1632 Size: 804 878 1317 1384 268 1917 Fragments arranged by size: 1917 1384 1317 878 804 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6371 AflIII A'CryG_T Cuts at: 1203 2980 5467 6444 1203 Size: 1777 2487 977 1327 Fragments arranged by size: 2487 1777 1327 977 AgeI A'CCGG_T Cuts at: 5399 5399 Size: 6568 AhdI GACnn_n'nnGTC Cuts at: 5297 5297 Size: 6568 AleI CACnn'nnGTG Cuts at: 1166 3812 1166 Size: 2646 3922 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6568 AlwNI CAG_nnn'CTG Cuts at: 4311 5883 4311 Size: 1572 4996 ApaLI G'TGCA_C Cuts at: 2115 4625 5781 2115 Size: 2510 1156 2902 Fragments arranged by size: 2902 2510 1156 ApoI r'AATT_y Cuts at: 1493 2087 3503 3877 4103 4255 6364 1493 Size: 594 1416 374 226 152 2109 1697 Fragments arranged by size: 2109 1697 1416 594 374 226 152 AseI AT'TA_AT Cuts at: 160 1032 5122 160 Size: 872 4090 1606 Fragments arranged by size: 4090 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6568 AvaI C'yCGr_G Cuts at: 1870 1943 2451 3893 1870 Size: 73 508 1442 4545 Fragments arranged by size: 4545 1442 508 73 BamHI G'GATC_C Cuts at: 3898 4265 3898 Size: 367 6201 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2200 2826 3202 3741 3889 Size: 325 217 627 413 626 376 539 148 Cuts at: 3889 5344 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 626 539 413 376 325 217 148 BanII G_rGCy'C Cuts at: 1391 2261 2436 3887 1391 Size: 870 175 1451 4072 Fragments arranged by size: 4072 1451 870 175 BbeI G_GCGC'C Cuts at: 2830 2830 Size: 6568 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2034 2201 3184 928 Size: 971 135 167 983 4312 Fragments arranged by size: 4312 983 971 167 135 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4759 4793 1903 Size: 34 2822 34 3678 Fragments arranged by size: 3678 2822 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4460 5670 4460 Size: 1210 5358 BclI T'GATC_A Cuts at: 1548 1548 Size: 6568 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5179 136 Size: 107 122 71 631 605 3507 1525 Fragments arranged by size: 3507 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2101 3437 6563 2101 Size: 1336 3126 2106 Fragments arranged by size: 3126 2106 1336 BlpI GC'TnA_GC Cuts at: 3482 3985 3482 Size: 503 6065 Bme1580I G_kGCm'C Cuts at: 1790 2119 4629 5785 1790 Size: 329 2510 1156 2573 Fragments arranged by size: 2573 2510 1156 329 BmgBI CAC'GTC Cuts at: 1318 2207 1318 Size: 889 5679 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2614 4299 5257 Size: 416 53 83 399 24 1197 1685 958 Cuts at: 5257 442 Size: 1753 Fragments arranged by size: 1753 1685 1197 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6568 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5228 1239 Size: 3989 2579 Bpu10I CC'TnA_GC Cuts at: 2334 3253 2334 Size: 919 5649 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3040 3142 4693 5558 5856 3040 Size: 102 1551 865 298 3752 Fragments arranged by size: 3752 1551 865 298 102 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2778 5231 882 Size: 617 1279 2453 2219 Fragments arranged by size: 2453 2219 1279 617 BsaAI yAC'GTr Cuts at: 493 1204 2981 4359 493 Size: 711 1777 1378 2702 Fragments arranged by size: 2702 1777 1378 711 BsaBI GATnn'nnATC Cuts at: 2106 6562 2106 Size: 4456 2112 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2827 4375 4757 5392 Size: 53 83 186 936 1294 1548 382 635 Cuts at: 5392 275 Size: 1451 Fragments arranged by size: 1548 1451 1294 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3723 5001 5399 5673 5820 6231 3723 Size: 1278 398 274 147 411 4060 Fragments arranged by size: 4060 1278 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6343 Fragments arranged by size: 6343 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6568 BseYI C'CCAG_C Cuts at: 5771 6267 5771 Size: 496 6072 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5995 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3724 4018 4779 5807 1540 Size: 275 186 1723 294 761 1028 2301 Fragments arranged by size: 2301 1723 1028 761 294 275 186 BsiHKAI G_wGCw'C Cuts at: 2119 4629 4714 5785 2119 Size: 2510 85 1071 2902 Fragments arranged by size: 2902 2510 1071 85 BsmI GAATG_Cn' Cuts at: 4079 4172 4079 Size: 93 6475 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2119 2261 2436 3887 4629 4714 5785 Size: 399 329 142 175 1451 742 85 1071 Cuts at: 5785 1391 Size: 2174 Fragments arranged by size: 2174 1451 1071 742 399 329 175 142 85 BspEI T'CCGG_A Cuts at: 6231 6231 Size: 6568 BspHI T'CATG_A Cuts at: 2082 3123 4457 6187 2082 Size: 1041 1334 1730 2463 Fragments arranged by size: 2463 1730 1334 1041 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2704 2888 2928 3923 844 Size: 1860 184 40 995 3489 Fragments arranged by size: 3489 1860 995 184 40 BsrBI CCG'CTC Cuts at: 1394 1823 4455 1394 Size: 429 2632 3507 Fragments arranged by size: 3507 2632 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2893 5063 5237 65 Size: 1183 1645 2170 174 1396 Fragments arranged by size: 2170 1645 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5212 5399 1700 Size: 110 3402 187 2869 Fragments arranged by size: 3402 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6568 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6568 BssSI C'ACGA_G Cuts at: 4628 5640 4628 Size: 1012 5556 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6568 BstBI TT'CG_AA Cuts at: 5372 5372 Size: 6568 BstEII G'GTnAC_C Cuts at: 1184 2462 1184 Size: 1278 5290 BstXI CCAn_nnnn'nTGG Cuts at: 2268 2268 Size: 6568 BstYI r'GATC_y Cuts at: 1972 2101 3437 3620 3898 4265 4650 4667 6119 Size: 129 1336 183 278 367 385 17 1452 Cuts at: 6119 6205 6217 6563 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1336 385 367 346 278 183 129 86 17 12 BstZ17I GTA'TAC Cuts at: 3321 3321 Size: 6568 BtgI C'CryG_G Cuts at: 513 1065 3600 513 Size: 552 2535 3481 Fragments arranged by size: 3481 2535 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5829 Fragments arranged by size: 5829 683 56 BtsI GCAGTG_nn' Cuts at: 4084 4878 4898 4084 Size: 794 20 5754 Fragments arranged by size: 5754 794 20 ClaI AT'CG_AT Cuts at: 1554 2292 1554 Size: 738 5830 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6533 DraI TTT'AAA Cuts at: 3270 3872 3270 Size: 602 5966 DraIII CAC_nnn'GTG Cuts at: 2622 2622 Size: 6568 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3188 5575 809 Size: 507 1872 2387 1802 Fragments arranged by size: 2387 1872 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2270 3385 4015 Size: 54 1425 50 275 18 440 1115 630 Cuts at: 4015 4904 6533 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1115 889 630 440 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4015 1537 Size: 275 2203 4090 Fragments arranged by size: 4090 2203 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2245 2606 3533 4498 1353 Size: 892 361 927 965 3423 Fragments arranged by size: 3423 965 927 892 361 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5155 5529 5675 387 Size: 1064 138 3566 374 146 1280 Fragments arranged by size: 3566 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2436 3314 4631 5228 6015 6283 1239 Size: 393 804 878 1317 597 787 268 1524 Fragments arranged by size: 1524 1317 878 804 787 597 393 268 EcoRI G'AATT_C Cuts at: 3877 4255 3877 Size: 378 6190 EcoRV GAT'ATC Cuts at: 3683 3683 Size: 6568 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2948 2948 Size: 6568 FspI TGC'GCA Cuts at: 5074 5074 Size: 6568 HaeII r_GCGC'y Cuts at: 1678 2830 5715 1678 Size: 1152 2885 2531 Fragments arranged by size: 2885 2531 1152 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2524 2556 2775 2807 3656 Size: 32 135 32 952 32 219 32 849 Cuts at: 3656 3688 3824 3856 5209 5241 5283 5315 1373 Size: 32 136 32 1353 32 42 32 2626 Fragments arranged by size: 2626 1353 952 849 219 136 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2683 3912 4158 669 Size: 988 281 745 1229 246 3079 Fragments arranged by size: 3079 1229 988 745 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6568 HpaI GTT'AAC Cuts at: 4158 4158 Size: 6568 KasI G'GCGC_C Cuts at: 2826 2826 Size: 6568 KpnI G_GTAC'C Cuts at: 3206 3893 3206 Size: 687 5881 MfeI C'AATT_G Cuts at: 2579 4167 2579 Size: 1588 4980 MluI A'CGCG_T Cuts at: 6444 6444 Size: 6568 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5682 5866 1305 Size: 4377 184 2007 Fragments arranged by size: 4377 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2272 3387 10 Size: 54 1425 343 440 1115 3191 Fragments arranged by size: 3191 1425 1115 440 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2112 2645 3410 3690 3812 4526 4885 Size: 648 946 533 765 280 122 714 359 Cuts at: 4885 5044 518 Size: 159 2042 Fragments arranged by size: 2042 946 765 714 648 533 359 280 159 122 MspA1I CmG'CkG Cuts at: 1606 2129 3984 4661 5809 6054 1606 Size: 523 1855 677 1148 245 2120 Fragments arranged by size: 2120 1855 1148 677 523 245 NarI GG'CG_CC Cuts at: 2827 2827 Size: 6568 NcoI C'CATG_G Cuts at: 513 1065 3600 513 Size: 552 2535 3481 Fragments arranged by size: 3481 2535 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6568 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6568 NotI GC'GGCC_GC Cuts at: 4015 4015 Size: 6568 NspI r_CATG'y Cuts at: 1132 1229 3926 5471 1132 Size: 97 2697 1545 2229 Fragments arranged by size: 2697 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6568 PciI A'CATG_T Cuts at: 5467 5467 Size: 6568 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6568 PmeI GTTT'AAAC Cuts at: 3872 3872 Size: 6568 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2063 2095 3038 3070 4637 4669 6150 6182 2063 Size: 32 943 32 1567 32 1481 32 2449 Fragments arranged by size: 2449 1567 1481 943 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6568 PsiI TTA'TAA Cuts at: 4138 4138 Size: 6568 PstI C_TGCA'G Cuts at: 830 3920 830 Size: 3090 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6568 PvuII CAG'CTG Cuts at: 2129 2129 Size: 6568 SalI G'TCGA_C Cuts at: 1936 3910 1936 Size: 1974 4594 SapI GCTCTTCn'nnn_ Cuts at: 3533 3533 Size: 6568 SbfI CC_TGCA'GG Cuts at: 3920 3920 Size: 6568 ScaI AGT'ACT Cuts at: 4816 6528 4816 Size: 1712 4856 SfcI C'TryA_G Cuts at: 826 1045 2543 2665 3916 5051 5732 5923 826 Size: 219 1498 122 1251 1135 681 191 1471 Fragments arranged by size: 1498 1471 1251 1135 681 219 191 122 SfoI GGC'GCC Cuts at: 2828 2828 Size: 6568 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6568 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6568 SmaI CCC'GGG Cuts at: 1872 3895 1872 Size: 2023 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3055 3157 4672 5573 5835 820 Size: 197 926 1112 102 1515 901 262 1553 Fragments arranged by size: 1553 1515 1112 926 901 262 197 102 SnaBI TAC'GTA Cuts at: 493 4359 493 Size: 3866 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6568 SphI G_CATG'C Cuts at: 1132 3926 1132 Size: 2794 3774 SspI AAT'ATT Cuts at: 5 52 4492 5 Size: 47 4440 2081 Fragments arranged by size: 4440 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6568 StyI C'CwwG_G Cuts at: 513 1065 2219 2388 2751 3600 4007 513 Size: 552 1154 169 363 849 407 3074 Fragments arranged by size: 3074 1154 849 552 407 363 169 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2188 2633 4595 4612 4765 4950 5395 Size: 1255 608 445 1962 17 153 185 445 Cuts at: 5395 325 Size: 1498 Fragments arranged by size: 1962 1498 1255 608 445 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4814 6526 96 Size: 274 80 33 51 1443 2837 1712 138 Fragments arranged by size: 2837 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6528 6560 6528 Size: 32 6536 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6568 XbaI T'CTAG_A Cuts at: 3842 3904 3842 Size: 62 6506 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6568 XmaI C'CCGG_G Cuts at: 1870 3893 1870 Size: 2023 4545 XmnI GAAnn'nnTTC Cuts at: 2103 3131 3303 3407 4697 2103 Size: 1028 172 104 1290 3974 Fragments arranged by size: 3974 1290 1028 172 104 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4376 276 Size: 53 83 186 936 2842 2468 Fragments arranged by size: 2842 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI