(Linear) (Six-base) MAPSORT of: KIEE6484.seq Check: 34 from: 1 to: 1863 KIEE6484.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AccI GT'mk_AC Cuts at: 0 659 1315 1863 Size: 659 656 548 Fragments arranged by size: 659 656 548 Acc65I G'GTAC_C Cuts at: 0 1197 1863 Size: 1197 666 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 431 1309 1863 Size: 431 878 554 Fragments arranged by size: 878 554 431 AflIII A'CryG_T Cuts at: 0 975 1863 Size: 975 888 AleI CACnn'nnGTG Cuts at: 0 1807 1863 Size: 1807 56 ApaLI G'TGCA_C Cuts at: 0 110 1863 Size: 110 1753 ApoI r'AATT_y Cuts at: 0 82 1498 1863 Size: 82 1416 365 Fragments arranged by size: 1416 365 82 AvaI C'yCGr_G Cuts at: 0 446 1863 Size: 446 1417 BanI G'GyrC_C Cuts at: 0 195 821 1197 1736 1863 Size: 195 626 376 539 127 Fragments arranged by size: 626 539 376 195 127 BanII G_rGCy'C Cuts at: 0 256 431 1863 Size: 256 175 1432 Fragments arranged by size: 1432 256 175 BbeI G_GCGC'C Cuts at: 0 825 1863 Size: 825 1038 BbsI GAAGACnn'nnnn_ Cuts at: 0 29 196 1179 1863 Size: 29 167 983 684 Fragments arranged by size: 983 684 167 29 BglII A'GATC_T Cuts at: 0 96 1432 1863 Size: 96 1336 431 Fragments arranged by size: 1336 431 96 BlpI GC'TnA_GC Cuts at: 0 1477 1863 Size: 1477 386 Bme1580I G_kGCm'C Cuts at: 0 114 1863 Size: 114 1749 BmgBI CAC'GTC Cuts at: 0 202 1863 Size: 202 1661 BmrI ACTGGGnnnn_n' Cuts at: 0 609 1863 Size: 609 1254 Bpu10I CC'TnA_GC Cuts at: 0 329 1248 1863 Size: 329 919 615 Fragments arranged by size: 919 615 329 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1035 1137 1863 Size: 1035 102 726 Fragments arranged by size: 1035 726 102 BsaI GGTCTCn'nnnn_ Cuts at: 0 773 1863 Size: 773 1090 BsaAI yAC'GTr Cuts at: 0 976 1863 Size: 976 887 BsaBI GATnn'nnATC Cuts at: 0 101 1863 Size: 101 1762 BsaHI Gr'CG_yC Cuts at: 0 822 1863 Size: 822 1041 BsaWI w'CCGG_w Cuts at: 0 1718 1863 Size: 1718 145 BsiEI CG_ry'CG Cuts at: 0 1719 1863 Size: 1719 144 BsiHKAI G_wGCw'C Cuts at: 0 114 1863 Size: 114 1749 Bsp1286I G_dGCh'C Cuts at: 0 114 256 431 1863 Size: 114 142 175 1432 Fragments arranged by size: 1432 175 142 114 BspHI T'CATG_A Cuts at: 0 77 1118 1863 Size: 77 1041 745 Fragments arranged by size: 1041 745 77 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 699 883 923 1863 Size: 699 184 40 940 Fragments arranged by size: 940 699 184 40 BsrDI GCAATG_nn' Cuts at: 0 888 1863 Size: 888 975 BstEII G'GTnAC_C Cuts at: 0 457 1863 Size: 457 1406 BstXI CCAn_nnnn'nTGG Cuts at: 0 263 1863 Size: 263 1600 BstYI r'GATC_y Cuts at: 0 96 1432 1615 1863 Size: 96 1336 183 248 Fragments arranged by size: 1336 248 183 96 BstZ17I GTA'TAC Cuts at: 0 1316 1863 Size: 1316 547 BtgI C'CryG_G Cuts at: 0 1595 1863 Size: 1595 268 ClaI AT'CG_AT Cuts at: 0 287 1863 Size: 287 1576 DraI TTT'AAA Cuts at: 0 1265 1863 Size: 1265 598 DraIII CAC_nnn'GTG Cuts at: 0 617 1863 Size: 617 1246 DrdI GACnn_nn'nnGTC Cuts at: 0 1183 1863 Size: 1183 680 EaeI y'GGCC_r Cuts at: 0 265 1380 1863 Size: 265 1115 483 Fragments arranged by size: 1115 483 265 EarI CTCTTCn'nnn_ Cuts at: 0 240 601 1528 1863 Size: 240 361 927 335 Fragments arranged by size: 927 361 335 240 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 431 1309 1863 Size: 431 878 554 Fragments arranged by size: 878 554 431 EcoRV GAT'ATC Cuts at: 0 1678 1863 Size: 1678 185 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 943 1863 Size: 943 920 HaeII r_GCGC'y Cuts at: 0 825 1863 Size: 825 1038 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 519 551 770 802 1651 1683 1819 1851 Size: 519 32 219 32 849 32 136 32 Cuts at: 1851 1863 Size: 12 Fragments arranged by size: 849 519 219 136 32 32 32 32 12 HincII GTy'rAC Cuts at: 0 678 1863 Size: 678 1185 KasI G'GCGC_C Cuts at: 0 821 1863 Size: 821 1042 KpnI G_GTAC'C Cuts at: 0 1201 1863 Size: 1201 662 MfeI C'AATT_G Cuts at: 0 574 1863 Size: 574 1289 MscI TGG'CCA Cuts at: 0 267 1382 1863 Size: 267 1115 481 Fragments arranged by size: 1115 481 267 MslI CAynn'nnrTG Cuts at: 0 107 640 1405 1685 1807 1863 Size: 107 533 765 280 122 56 Fragments arranged by size: 765 533 280 122 107 56 MspA1I CmG'CkG Cuts at: 0 124 1863 Size: 124 1739 NarI GG'CG_CC Cuts at: 0 822 1863 Size: 822 1041 NcoI C'CATG_G Cuts at: 0 1595 1863 Size: 1595 268 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 58 90 1033 1065 1863 Size: 58 32 943 32 798 Fragments arranged by size: 943 798 58 32 32 PvuII CAG'CTG Cuts at: 0 124 1863 Size: 124 1739 SapI GCTCTTCn'nnn_ Cuts at: 0 1528 1863 Size: 1528 335 SfcI C'TryA_G Cuts at: 0 538 660 1863 Size: 538 122 1203 Fragments arranged by size: 1203 538 122 SfoI GGC'GCC Cuts at: 0 823 1863 Size: 823 1040 SmlI C'TyrA_G Cuts at: 0 1050 1152 1863 Size: 1050 102 711 Fragments arranged by size: 1050 711 102 StyI C'CwwG_G Cuts at: 0 214 383 746 1595 1863 Size: 214 169 363 849 268 Fragments arranged by size: 849 363 268 214 169 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 183 628 1863 Size: 183 445 1235 Fragments arranged by size: 1235 445 183 XbaI T'CTAG_A Cuts at: 0 1837 1863 Size: 1837 26 XmnI GAAnn'nnTTC Cuts at: 0 98 1126 1298 1402 1863 Size: 98 1028 172 104 461 Fragments arranged by size: 1028 461 172 104 98 Enzymes that do cut: AccI Acc65I AcuI AflIII AleI ApaLI ApoI AvaI BanI BanII BbeI BbsI BglII BlpI Bme1580I BmgBI BmrI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrDI BstEII BstXI BstYI BstZ17I BtgI ClaI DraI DraIII DrdI EaeI EarI Eco57MI EcoRV FalI HaeII Hin4I HincII KasI KpnI MfeI MscI MslI MspA1I NarI NcoI PpiI PvuII SapI SfcI SfoI SmlI StyI TaqII XbaI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AflII AgeI AhdI AlfI AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI BmtI BplI BpmI BsaXI BseRI BseYI BsgI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI Bsu36I BtgZI BtsI CspCI EagI EciI EcoICRI EcoNI EcoO109I EcoRI FseI FspI FspAI HindIII HpaI MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XcmI XhoI XmaI ZraI