################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BanII BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BspDI BspEI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MaeIII MamI MfeI MluI MluNI MroI MscI MslI MunI NciI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfuI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AatII 709 GACGTC GACGT^C AluI 83 AGCT AG^CT Alw44I 109 GTGCAC G^TGCAC AlwI 210 GATCC GGATC(4/5) AlwNI 162 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 109 GTGCAC G^TGCAC AspHI 109 GTGCAC G[AT]GC[AT]^C AvaI 8 CCCGAG C^[CT]CG[GA]G BciVI 523 GGATAC GTATCC(6/5) BfaI 448 CTAG C^TAG BsiHKAI 109 GTGCAC G[AT]GC[AT]^C BsoBI 8 CCCGAG C^[CT]CG[GA]G Bsp1407I 284 TGTACA T^GTACA BsrGI 284 TGTACA T^GTACA BstF5I 782 GGATG GGATG(2/0) BstXI 335 CCATGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 209 AGATCC [GA]^GATC[CT] DsaI 335 CCATGG C^C[GA][CT]GG EarI 190 CTCTTC CTCTTC(1/4) EcoNI 393 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FokI 782 GGATG GGATG(9/13) HincII 154 GTCAAC GT[CT]^[GA]AC HindII 154 GTCAAC GT[CT]^[GA]AC Ksp632I 190 CTCTTC CTCTTC(1/4) MaeI 448 CTAG C^TAG MflI 209 AGATCC [GA]^GATC[CT] MspA1I 165 CCGCTG C[AC]G^C[GT]G NcoI 335 CCATGG C^CATGG PleI 139 GACTC GAGTC(4/5) SapI 189 GCTCTTC GCTCTTC(1/4) SgrAI 246 CGCCGGCG C[GA]^CCGG[CT]G SspBI 284 TGTACA T^GTACA TaqI 383 TCGA T^CGA TfiI 515 GAATC G^A[AT]TC TthHB8I 383 TCGA T^CGA XcmI 161 CCAGCCGCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 209 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AfaI 258 GTAC GT^AC 285 GTAC BglI 13 GCCGCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 295 GCCGAGGTGGC BmyI 109 GTGCAC G[AGT]GC[ACT]^C 264 GGGCAC BpmI 141 CTCCAG CTGGAG(16/14) 121 CTGGAG BseRI 37 CTCCTC GAGGAG(10/8) 391 CTCCTC Bsp1286I 109 GTGCAC G[AGT]GC[ACT]^C 264 GGGCAC BspHI 311 TCATGA T^CATGA 351 TCATGA BsrFI 116 GCCGGC [GA]^CCGG[CT] 247 GCCGGC Cfr10I 116 GCCGGC [GA]^CCGG[CT] 247 GCCGGC Cfr13I 442 GGGCC G^G[ACGT]CC 776 GGGCC DdeI 418 CTGAG C^T[ACGT]AG 689 CTCAG DpnII 210 GATC ^GATC 381 GATC EcoT14I 197 CCAAGG C^C[AT][AT]GG 335 CCATGG FauI 25 CCCGC CCCGC(4/6) 18 GCGGG HapII 117 CCGG C^CGG 248 CCGG HinfI 139 GACTC G^A[ACGT]TC 515 GAATC HpaII 117 CCGG C^CGG 248 CCGG MboI 210 GATC ^GATC 381 GATC MseI 431 TTAA T^TAA 619 TTAA MspI 117 CCGG C^CGG 248 CCGG NaeI 116 GCCGGC GCC^GGC 247 GCCGGC NdeII 210 GATC ^GATC 381 GATC NgoMIV 116 GCCGGC G^CCGGC 247 GCCGGC RcaI 311 TCATGA T^CATGA 351 TCATGA RsaI 258 GTAC GT^AC 285 GTAC Sau3AI 210 GATC ^GATC 381 GATC Sau96I 442 GGGCC G^G[ACGT]CC 776 GGGCC SfaNI 331 GATGC GCATC(5/9) 451 GCATC StyI 197 CCAAGG C^C[AT][AT]GG 335 CCATGG Tru9I 431 TTAA T^TAA 619 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AccII AciI AcsI AcyI ApoI BanI BbeI BbvI BsaHI BsaJI BsiYI BslI BsmFI BsrI BssKI BstNI BstUI Cac8I CfoI EcoRII Fnu4HI HaeII HaeIII HgaI HhaI Hin1I HinP1I HphI ItaI KasI MaeII MboII MnlI MvaI MvnI MwoI NarI NlaIII NlaIV ScrFI SfoI TseI Tsp509I TspRI