(Circular) (Six-base) MAPSORT of: pFN21AE5339.seq Check: 5829 from: 1 to: 5497 pFN21AE5339 5497 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2718 3307 278 Size: 53 83 186 936 1182 589 2468 Fragments arranged by size: 2468 1182 936 589 186 83 53 AccI GT'mk_AC Cuts at: 1937 2840 1937 Size: 903 4594 Acc65I G'GTAC_C Cuts at: 2818 2818 Size: 5497 AclI AA'CG_TT Cuts at: 1563 3624 3997 1563 Size: 2061 373 3063 Fragments arranged by size: 3063 2061 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2631 3560 4944 5212 1632 Size: 999 929 1384 268 1917 Fragments arranged by size: 1917 1384 999 929 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5300 AflIII A'CryG_T Cuts at: 1203 4396 5373 1203 Size: 3193 977 1327 Fragments arranged by size: 3193 1327 977 AgeI A'CCGG_T Cuts at: 4328 4328 Size: 5497 AhdI GACnn_n'nnGTC Cuts at: 4226 4226 Size: 5497 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5497 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5497 AlwNI CAG_nnn'CTG Cuts at: 2172 3240 4812 2172 Size: 1068 1572 2857 Fragments arranged by size: 2857 1572 1068 ApaLI G'TGCA_C Cuts at: 2114 3554 4710 2114 Size: 1440 1156 2901 Fragments arranged by size: 2901 1440 1156 ApoI r'AATT_y Cuts at: 1493 2360 2411 2572 2806 3032 3184 5293 1493 Size: 867 51 161 234 226 152 2109 1697 Fragments arranged by size: 2109 1697 867 234 226 161 152 51 AseI AT'TA_AT Cuts at: 160 1032 4051 160 Size: 872 3019 1606 Fragments arranged by size: 3019 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5497 AvaI C'yCGr_G Cuts at: 1870 1943 2013 2822 1870 Size: 73 70 809 4545 Fragments arranged by size: 4545 809 73 70 BamHI G'GATC_C Cuts at: 2827 3194 2827 Size: 367 5130 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2026 2078 2297 2818 4273 Size: 325 217 627 239 52 219 521 1455 Cuts at: 4273 618 Size: 1842 Fragments arranged by size: 1842 1455 627 521 325 239 219 217 52 BanII G_rGCy'C Cuts at: 1391 2816 1391 Size: 1425 4072 BbeI G_GCGC'C Cuts at: 2030 2082 2301 2030 Size: 52 219 5226 Fragments arranged by size: 5226 219 52 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4526 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3688 3722 1903 Size: 34 1751 34 3678 Fragments arranged by size: 3678 1751 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2522 3389 4599 2522 Size: 867 1210 3420 Fragments arranged by size: 3420 1210 867 BclI T'GATC_A Cuts at: 1548 1548 Size: 5497 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2024 2306 4108 Size: 107 122 71 631 605 352 282 1802 Cuts at: 4108 136 Size: 1525 Fragments arranged by size: 1802 1525 631 605 352 282 122 107 71 BglII A'GATC_T Cuts at: 5492 5492 Size: 5497 BlpI GC'TnA_GC Cuts at: 2914 2914 Size: 5497 Bme1580I G_kGCm'C Cuts at: 1790 2118 2273 3558 4714 1790 Size: 328 155 1285 1156 2573 Fragments arranged by size: 2573 1285 1156 328 155 BmgBI CAC'GTC Cuts at: 1318 2113 1318 Size: 795 4702 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2357 2666 3228 Size: 416 53 83 399 24 940 309 562 Cuts at: 3228 4186 442 Size: 958 1753 Fragments arranged by size: 1753 958 940 562 416 399 309 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5497 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2131 2147 4157 1239 Size: 892 16 2010 2579 Fragments arranged by size: 2579 2010 892 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3622 4487 4785 3622 Size: 865 298 4334 Fragments arranged by size: 4334 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4160 882 Size: 617 2661 2219 Fragments arranged by size: 2661 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3288 493 Size: 711 2084 2702 Fragments arranged by size: 2702 2084 711 BsaBI GATnn'nnATC Cuts at: 5491 5491 Size: 5497 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2027 2079 2250 2298 Size: 53 83 186 936 494 52 171 48 Cuts at: 2298 2715 3304 3686 4321 275 Size: 417 589 382 635 1451 Fragments arranged by size: 1451 936 635 589 494 417 382 186 171 83 53 52 48 BsaWI w'CCGG_w Cuts at: 3930 4328 4602 4749 5160 3930 Size: 398 274 147 411 4267 Fragments arranged by size: 4267 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5272 Fragments arranged by size: 5272 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2387 1186 Size: 847 354 4296 Fragments arranged by size: 4296 847 354 BseYI C'CCAG_C Cuts at: 4700 5196 4700 Size: 496 5001 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4924 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2947 3708 4736 1540 Size: 275 186 946 761 1028 2301 Fragments arranged by size: 2301 1028 946 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2118 3558 3643 4714 2118 Size: 1440 85 1071 2901 Fragments arranged by size: 2901 1440 1071 85 BsmI GAATG_Cn' Cuts at: 3008 3101 3008 Size: 93 5404 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2273 2816 3558 3643 4714 1391 Size: 399 328 155 543 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 543 399 328 155 85 BspEI T'CCGG_A Cuts at: 5160 5160 Size: 5497 BspHI T'CATG_A Cuts at: 2316 2356 3386 5116 2316 Size: 40 1030 1730 2697 Fragments arranged by size: 2697 1730 1030 40 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2852 844 Size: 2008 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3384 1394 Size: 429 1561 3507 Fragments arranged by size: 3507 1561 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3992 4166 65 Size: 1183 2744 174 1396 Fragments arranged by size: 2744 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2121 2252 4141 4328 1700 Size: 110 311 131 1889 187 2869 Fragments arranged by size: 2869 1889 311 187 131 110 BsrGI T'GTAC_A Cuts at: 96 2289 96 Size: 2193 3304 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5497 BssSI C'ACGA_G Cuts at: 3557 4569 3557 Size: 1012 4485 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5497 BstBI TT'CG_AA Cuts at: 4301 4301 Size: 5497 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5497 BstXI CCAn_nnnn'nTGG Cuts at: 2347 2347 Size: 5497 BstYI r'GATC_y Cuts at: 1972 2214 2827 3194 3579 3596 5048 5134 5146 Size: 242 613 367 385 17 1452 86 12 Cuts at: 5146 5492 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 613 385 367 346 242 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2340 513 Size: 552 1275 3670 Fragments arranged by size: 3670 1275 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2674 487 Size: 683 56 1448 3310 Fragments arranged by size: 3310 1448 683 56 BtsI GCAGTG_nn' Cuts at: 2595 3013 3807 3827 2595 Size: 418 794 20 4265 Fragments arranged by size: 4265 794 418 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5497 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5462 DraI TTT'AAA Cuts at: 2801 2801 Size: 5497 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4504 809 Size: 507 3188 1802 Fragments arranged by size: 3188 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2944 3833 5462 Size: 54 1425 50 275 18 1114 889 1629 Cuts at: 5462 8 Size: 43 Fragments arranged by size: 1629 1425 1114 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2944 1537 Size: 275 1132 4090 Fragments arranged by size: 4090 1132 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2201 3427 1353 Size: 848 1226 3423 Fragments arranged by size: 3423 1226 848 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2207 4084 4458 4604 387 Size: 1064 138 618 1877 374 146 1280 Fragments arranged by size: 1877 1280 1064 618 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2131 2147 2631 3560 4157 4944 5212 Size: 393 499 16 484 929 597 787 268 Cuts at: 5212 1239 Size: 1524 Fragments arranged by size: 1524 929 787 597 499 484 393 268 16 EcoNI CCTnn'n_nnAGG Cuts at: 2402 2402 Size: 5497 EcoRI G'AATT_C Cuts at: 2806 3184 2806 Size: 378 5119 FspI TGC'GCA Cuts at: 4003 4003 Size: 5497 HaeII r_GCGC'y Cuts at: 1678 2030 2082 2301 4644 1678 Size: 352 52 219 2343 2531 Fragments arranged by size: 2531 2343 352 219 52 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4138 4170 4212 4244 1373 Size: 32 135 32 2566 32 42 32 2626 Fragments arranged by size: 2626 2566 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2161 2841 3087 669 Size: 988 281 223 680 246 3079 Fragments arranged by size: 3079 988 680 281 246 223 HindIII A'AGCT_T Cuts at: 748 748 Size: 5497 HpaI GTT'AAC Cuts at: 3087 3087 Size: 5497 KasI G'GCGC_C Cuts at: 2026 2078 2297 2026 Size: 52 219 5226 Fragments arranged by size: 5226 219 52 KpnI G_GTAC'C Cuts at: 2822 2822 Size: 5497 MfeI C'AATT_G Cuts at: 3096 3096 Size: 5497 MluI A'CGCG_T Cuts at: 5373 5373 Size: 5497 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2506 2760 4611 4795 1305 Size: 1201 254 1851 184 2007 Fragments arranged by size: 2007 1851 1201 254 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3675 Fragments arranged by size: 3675 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3455 3814 3973 518 Size: 648 2289 359 159 2042 Fragments arranged by size: 2289 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2172 2913 3590 4738 4983 1606 Size: 566 741 677 1148 245 2120 Fragments arranged by size: 2120 1148 741 677 566 245 NaeI GCC'GGC Cuts at: 2123 2254 2123 Size: 131 5366 NarI GG'CG_CC Cuts at: 2027 2079 2298 2027 Size: 52 219 5226 Fragments arranged by size: 5226 219 52 NcoI C'CATG_G Cuts at: 513 1065 2340 513 Size: 552 1275 3670 Fragments arranged by size: 3670 1275 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5497 NgoMIV G'CCGG_C Cuts at: 2121 2252 2121 Size: 131 5366 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5497 NotI GC'GGCC_GC Cuts at: 2944 2944 Size: 5497 NspI r_CATG'y Cuts at: 1132 1229 2855 4400 1132 Size: 97 1626 1545 2229 Fragments arranged by size: 2229 1626 1545 97 PasI CC'CwG_GG Cuts at: 1348 2050 1348 Size: 702 4795 PciI A'CATG_T Cuts at: 4396 4396 Size: 5497 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5497 PmeI GTTT'AAAC Cuts at: 2801 2801 Size: 5497 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3566 3598 5079 5111 3566 Size: 32 1481 32 3952 Fragments arranged by size: 3952 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5497 PsiI TTA'TAA Cuts at: 3067 3067 Size: 5497 PstI C_TGCA'G Cuts at: 830 2849 830 Size: 2019 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5497 SalI G'TCGA_C Cuts at: 1936 2839 1936 Size: 903 4594 SapI GCTCTTCn'nnn_ Cuts at: 2201 2201 Size: 5497 SbfI CC_TGCA'GG Cuts at: 2849 2849 Size: 5497 ScaI AGT'ACT Cuts at: 3745 5457 3745 Size: 1712 3785 SfcI C'TryA_G Cuts at: 826 1045 2845 3980 4661 4852 826 Size: 219 1800 1135 681 191 1471 Fragments arranged by size: 1800 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2028 2080 2299 2028 Size: 52 219 5226 Fragments arranged by size: 5226 219 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5497 SgrAI Cr'CCGG_yG Cuts at: 1700 2252 1700 Size: 552 4945 SmaI CCC'GGG Cuts at: 1872 2824 1872 Size: 952 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3601 4502 4764 820 Size: 197 926 1658 901 262 1553 Fragments arranged by size: 1658 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3288 493 Size: 2795 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5497 SphI G_CATG'C Cuts at: 1132 2855 1132 Size: 1723 3774 SspI AAT'ATT Cuts at: 5 52 3421 5 Size: 47 3369 2081 Fragments arranged by size: 3369 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5497 StyI C'CwwG_G Cuts at: 513 1065 2202 2340 2936 513 Size: 552 1137 138 596 3074 Fragments arranged by size: 3074 1137 596 552 138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2168 3524 3541 3694 3879 4324 325 Size: 1255 588 1356 17 153 185 445 1498 Fragments arranged by size: 1498 1356 1255 588 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2289 3743 5455 Size: 274 80 33 51 1443 312 1454 1712 Cuts at: 5455 96 Size: 138 Fragments arranged by size: 1712 1454 1443 312 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5457 5489 5457 Size: 32 5465 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5497 XbaI T'CTAG_A Cuts at: 2833 2833 Size: 5497 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2173 2173 Size: 5497 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5497 XmaI C'CCGG_G Cuts at: 1870 2822 1870 Size: 952 4545 XmnI GAAnn'nnTTC Cuts at: 3626 3626 Size: 5497 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2716 3305 276 Size: 53 83 186 936 1182 589 2468 Fragments arranged by size: 2468 1182 936 589 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI