################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI BspDI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MscI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 65 enzymes) # ################################### AccIII 740 TCCGGA T^CCGGA AciI 423 GCGG CCGC(-3/-1) AcsI 712 AAATTC [GA]^AATT[CT] AfaI 247 GTAC GT^AC AocI 33 CCTGAGG CC^T[ACGT]AGG ApoI 712 AAATTC [GA]^AATT[CT] Asp700I 732 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 115 GAGCAC G[AT]GC[AT]^C AvaII 631 GGACC G^G[AT]CC BanII 27 GAGCCC G[GA]GC[CT]^C BbsI 646 GAAGAC GAAGAC(2/6) BbvI 475 GCTGC GCAGC(8/12) BfaI 635 CTAG C^TAG BglII 768 AGATCT A^GATCT BpmI 434 CTGGAG CTGGAG(16/14) BpuAI 646 GAAGAC GAAGAC(2/6) BsaWI 740 TCCGGA [AT]^CCGG[AT] BseAI 740 TCCGGA T^CCGGA BsiHKAI 115 GAGCAC G[AT]GC[AT]^C BsiYI 433 CCTGGAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 433 CCTGGAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 789 GTCTC GTCTC(1/5) Bsp1407I 246 TGTACA T^GTACA BspEI 740 TCCGGA T^CCGGA BsrDI 190 GCAATG GCAATG(2/0) BsrGI 246 TGTACA T^GTACA BssKI 433 CCTGG ^CC[ACGT]GG BstNI 433 CCTGG CC^[AT]GG BstYI 768 AGATCT [GA]^GATC[CT] Bsu36I 33 CCTGAGG CC^T[ACGT]AGG Cac8I 666 GCAAGC GC[ACGT]^[ACGT]GC CfoI 568 GCGC GCG^C DpnII 769 GATC ^GATC Eco81I 33 CCTGAGG CC^T[ACGT]AGG EcoRII 433 CCTGG ^CC[AT]GG EcoT14I 264 CCTTGG C^C[AT][AT]GG Fnu4HI 475 GCTGC GC^[ACGT]GC HaeII 567 AGCGCC [GA]GCGC^[CT] HapII 741 CCGG C^CGG HhaI 568 GCGC GCG^C HinP1I 568 GCGC G^CGC HpaII 741 CCGG C^CGG ItaI 475 GCTGC GC^[ACGT]GC MaeI 635 CTAG C^TAG MaeIII 220 GTAAC ^GT[ACGT]AC MboI 769 GATC ^GATC MflI 768 AGATCT [GA]^GATC[CT] MroI 740 TCCGGA T^CCGGA MspI 741 CCGG C^CGG MvaI 433 CCTGG CC^[AT]GG NdeII 769 GATC ^GATC PleI 370 GAGTC GAGTC(4/5) PpuMI 630 AGGACCT [GA]G^G[AT]CC[CT] RsaI 247 GTAC GT^AC Sau3AI 769 GATC ^GATC ScrFI 433 CCTGG CC^[ACGT]GG SfaNI 364 GATGC GCATC(5/9) SfcI 641 CTGTAG C^T[GA][CT]AG SspBI 246 TGTACA T^GTACA StyI 264 CCTTGG C^C[AT][AT]GG TaqI 10 TCGA T^CGA TseI 475 GCTGC G^C[AT]GC TthHB8I 10 TCGA T^CGA XhoII 768 AGATCT [GA]^GATC[CT] XmnI 732 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AflII 297 CTTAAG C^TTAAG 405 CTTAAG AluI 351 AGCT AG^CT 555 AGCT BfrI 297 CTTAAG C^TTAAG 405 CTTAAG BmyI 27 GAGCCC G[AGT]GC[ACT]^C 115 GAGCAC BsaJI 264 CCTTGG C^C[ACGT][ACGT]GG 432 CCCTGG BseRI 385 GAGGAG GAGGAG(10/8) 388 GAGGAG Bsp1286I 27 GAGCCC G[AGT]GC[ACT]^C 115 GAGCAC DdeI 34 CTGAG C^T[ACGT]AG 345 CTTAG DraII 37 AGGCCCC [GA]G^G[ACGT]CC[CT] 630 AGGACCT EcoO109I 37 AGGCCCC [GA]G^G[ACGT]CC[CT] 630 AGGACCT MslI 18 CATGGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 328 CATATTAGTG NlaIII 18 CATG CATG^ 24 CATG NlaIV 38 GGCCCC GG[ACGT]^[ACGT]CC 450 GGTTCC SmlI 297 CTTAAG C^T[CT][GA]AG 405 CTTAAG TfiI 7 GATTC G^A[AT]TC 589 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### BsrI BstF5I Cfr13I EarI FokI HaeIII HinfI Ksp632I MboII MnlI MseI Sau96I Tru9I Tsp509I TspRI