################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BcgI BclI BfrI BglI BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoT22I FbaI FokI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 331 CGCG CG^CG AcsI 729 GAATTC [GA]^AATT[CT] AlwI 234 GGATC GGATC(4/5) AlwNI 444 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 591 GGGCCC GGGCC^C ApoI 729 GAATTC [GA]^AATT[CT] AvaI 595 CCCGGG C^[CT]CG[GA]G AvaII 174 GGACC G^G[AT]CC BanII 591 GGGCCC G[GA]GC[CT]^C BbsI 382 GTCTTC GAAGAC(2/6) BbvCI 427 GCTGAGG CCTCAGC(-5/-2) BciVI 256 GGATAC GTATCC(6/5) BfaI 148 CTAG C^TAG BglII 287 AGATCT A^GATCT BlpI 183 GCTCAGC GC^T[ACGT]AGC Bpu1102I 183 GCTCAGC GC^T[ACGT]AGC BpuAI 382 GTCTTC GAAGAC(2/6) BsmFI 539 GGGAC GGGAC(10/14) BsoBI 595 CCCGGG C^[CT]CG[GA]G BsrBI 181 CCGCTC CCGCTC(-3/-3) BssSI 205 CACGAG CACGAG(-5/-1) BstUI 331 CGCG CG^CG BstYI 287 AGATCT [GA]^GATC[CT] CelII 183 GCTCAGC GC^T[ACGT]AGC DraII 591 GGGCCCC [GA]G^G[ACGT]CC[CT] DsaI 75 CCACGG C^C[GA][CT]GG EcoNI 759 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 591 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoRI 729 GAATTC G^AATTC EcoRV 577 GATATC GAT^ATC EcoT14I 419 CCAAGG C^C[AT][AT]GG MaeI 148 CTAG C^TAG MaeIII 523 GTGAC ^GT[ACGT]AC MflI 287 AGATCT [GA]^GATC[CT] MslI 582 CACAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 331 CGCG CG^CG NspI 496 GCATGT [GA]CATG^[CT] PshAI 310 GACTTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 190 CAGCTG CAG^CTG SexAI 128 ACCTGGT A^CC[AT]GGT SmaI 595 CCCGGG CCC^GGG StyI 419 CCAAGG C^C[AT][AT]GG Tsp45I 523 GTGAC ^GT[GC]AC XcmI 443 CCAGCTTCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 287 AGATCT [GA]^GATC[CT] XmaCI 595 CCCGGG C^CCGGG XmaI 595 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### Alw44I 64 GTGCAC G^TGCAC 119 GTGCAC ApaLI 64 GTGCAC G^TGCAC 119 GTGCAC AspHI 64 GTGCAC G[AT]GC[AT]^C 119 GTGCAC BanI 347 GGCACC G^G[CT][GA]CC 695 GGCACC BseRI 400 CTCCTC GAGGAG(10/8) 208 GAGGAG BsgI 709 CTGCAC GTGCAG(16/14) 151 GTGCAG BsiHKAI 64 GTGCAC G[AT]GC[AT]^C 119 GTGCAC BsmBI 775 CGTCTC CGTCTC(1/5) 157 GAGACG BsrFI 60 GCCGGT [GA]^CCGG[CT] 548 ACCGGC Cfr10I 60 GCCGGT [GA]^CCGG[CT] 548 ACCGGC DpnII 235 GATC ^GATC 288 GATC FauI 180 CCCGC CCCGC(4/6) 83 GCGGG HaeII 86 GGCGCT [GA]GCGC^[CT] 488 GGCGCT HgaI 329 GACGC GACGC(5/10) 774 GCGTC HinfI 101 GAGTC G^A[ACGT]TC 646 GAGTC MboI 235 GATC ^GATC 288 GATC MboII 383 TCTTC GAAGA(8/7) 608 TCTTC NdeII 235 GATC ^GATC 288 GATC PflMI 319 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 567 CCAGTGGGTGG PleI 101 GAGTC GAGTC(4/5) 646 GAGTC Sau3AI 235 GATC ^GATC 288 GATC Tsp509I 601 AATT ^AATT 730 AATT Van91I 319 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 567 CCAGTGGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AluI BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PstI Sau96I ScrFI SfcI TseI TspRI