################################## # NO CUT ENZYMES ( 224 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AfaI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HaeIII HapII HhaI HinP1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI Ksp632I KspI MaeI MamI MboII MfeI MflI MluI MluNI MroI MscI MseI MspA1I MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI ScrFI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI Tru9I TseI Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AciI 98 CCGC CCGC(-3/-1) AcyI 76 GGCGTC G[GA]^CG[CT]C AflIII 62 ACGTGT A^C[GA][CT]GT AluI 11 AGCT AG^CT BanII 55 GGGCTC G[GA]GC[CT]^C BmyI 55 GGGCTC G[AGT]GC[ACT]^C BsaAI 61 TACGTG [CT]AC^GT[GA] BsaHI 76 GGCGTC G[GA]^CG[CT]C BsmBI 93 CGTCTC CGTCTC(1/5) BsmFI 42 GTCCC GGGAC(10/14) Bsp1286I 55 GGGCTC G[AGT]GC[ACT]^C BspMI 34 ACCTGC ACCTGC(4/8) BsrI 105 CCAGT ACTGG(1/-1) BstXI 45 CCACAGGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DpnII 139 GATC ^GATC HgaI 77 GCGTC GACGC(5/10) Hin1I 76 GGCGTC G[GA]^CG[CT]C HphI 86 TCACC GGTGA(8/7) MaeII 62 ACGT A^CGT MaeIII 85 GTCAC ^GT[ACGT]AC MboI 139 GATC ^GATC MslI 46 CACAGGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeII 139 GATC ^GATC NlaIII 148 CATG CATG^ NlaIV 131 GGAGCC GG[ACGT]^[ACGT]CC Sau3AI 139 GATC ^GATC SspI 175 AATATT AAT^ATT Tsp45I 85 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 5 enzymes) # ################################### HinfI 160 GATTC G^A[ACGT]TC 198 GAATC MnlI 115 GAGG CCTC(7/6) 210 GAGG MwoI 3 GCTGTTCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 68 GCTTCTTGGGC SfcI 16 CTACAG C^T[GA][CT]AG 165 CTATAG TfiI 160 GATTC G^A[AT]TC 198 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 1 enzymes) # ######################################### BsmAI