################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## Acc65I AccIII AclI AfaI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BbrPI BbsI BbvCI BciVI BclI BfrI BglII BlnI BpuAI BsaAI BsaBI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MamI MluI MluNI MroI MscI NcoI NdeI NheI NotI NruI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 1190 GACGTC GACGT^C AccI 1261 GTCTAC GT^[AC][GT]AC AccII 16 CGCG CG^CG Alw44I 1237 GTGCAC G^TGCAC ApaI 208 GGGCCC GGGCC^C ApaLI 1237 GTGCAC G^TGCAC AvaI 332 CTCGAG C^[CT]CG[GA]G BamHI 1168 GGATCC G^GATCC BanI 912 GGCGCC G^G[CT][GA]CC BbeI 912 GGCGCC GGCGC^C BcnI 1288 CCCGG CC^[GC]GG BglI 238 GCCTCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 598 GCTCAGC GC^T[ACGT]AGC Bpu1102I 598 GCTCAGC GC^T[ACGT]AGC BsaI 573 GAGACC GGTCTC(1/5) BsaWI 1196 TCCGGT [AT]^CCGG[AT] BseRI 880 CTCCTC GAGGAG(10/8) BsmBI 1192 CGTCTC CGTCTC(1/5) BsoBI 332 CTCGAG C^[CT]CG[GA]G BspHI 305 TCATGA T^CATGA BsrBI 954 CCGCTC CCGCTC(-3/-3) BsrFI 1084 GCCGGC [GA]^CCGG[CT] BssSI 604 CTCGTG CACGAG(-5/-1) BstUI 16 CGCG CG^CG BstXI 258 CCAAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1168 GGATCC [GA]^GATC[CT] CelII 598 GCTCAGC GC^T[ACGT]AGC Cfr10I 1084 GCCGGC [GA]^CCGG[CT] EcoNI 287 CCTGTAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1131 ATGCAT ATGCA^T HgaI 1248 GACGC GACGC(5/10) KasI 912 GGCGCC G^GCGCC MfeI 535 CAATTG C^AATTG MflI 1168 GGATCC [GA]^GATC[CT] MslI 838 CATCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 535 CAATTG C^AATTG MvnI 16 CGCG CG^CG NaeI 1084 GCCGGC GCC^GGC NarI 912 GGCGCC GG^CGCC NciI 1288 CCCGG CC^[GC]GG NgoMIV 1084 GCCGGC G^CCGGC NsiI 1131 ATGCAT ATGCA^T PaeR7I 332 CTCGAG C^TCGAG PpuMI 61 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 1186 GACGGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 305 TCATGA T^CATGA SfoI 912 GGCGCC GGC^GCC SmlI 332 CTCGAG C^T[CT][GA]AG XcmI 1128 CCAATGCATCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 332 CTCGAG C^TCGAG XhoII 1168 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcyI 912 GGCGCC G[GA]^CG[CT]C 1190 GACGTC BsaHI 912 GGCGCC G[GA]^CG[CT]C 1190 GACGTC BstF5I 1318 CATCC GGATG(2/0) 843 GGATG DraII 61 AGGTCCC [GA]G^G[ACGT]CC[CT] 620 GGGGCCT DsaI 482 CCACGG C^C[GA][CT]GG 1172 CCGTGG EcoO109I 61 AGGTCCC [GA]G^G[ACGT]CC[CT] 620 GGGGCCT FauI 902 CCCGC CCCGC(4/6) 562 GCGGG FokI 1318 CATCC GGATG(9/13) 843 GGATG HaeII 477 AGCGCC [GA]GCGC^[CT] 912 GGCGCC Hin1I 912 GGCGCC G[GA]^CG[CT]C 1190 GACGTC MaeII 734 ACGT A^CGT 1191 ACGT MseI 425 TTAA T^TAA 1358 TTAA MspA1I 184 CAGCTG C[AC]G^C[GT]G 263 CAGCTG NlaIII 306 CATG CATG^ 1056 CATG PleI 4 GAGTC GAGTC(4/5) 1275 GAGTC PvuII 184 CAGCTG CAG^CTG 263 CAGCTG SacI 805 GAGCTC GAGCT^C 963 GAGCTC Tru9I 425 TTAA T^TAA 1358 TTAA Tsp45I 1161 GTCAC ^GT[GC]AC 1277 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AspHI AvaII BanII BbvI BcgI BfaI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MspI MvaI MwoI NdeII NlaIV PstI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI TseI Tsp509I TspRI TthHB8I