(Circular) (Six-base) MAPSORT of: pFN21AE5574.seq Check: 9866 from: 1 to: 6067 pFN21AE5574 6067 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3199 3877 278 Size: 53 83 186 936 1663 678 2468 Fragments arranged by size: 2468 1663 936 678 186 83 53 AccI GT'mk_AC Cuts at: 1937 3267 3410 1937 Size: 1330 143 4594 Fragments arranged by size: 4594 1330 143 Acc65I G'GTAC_C Cuts at: 3388 3388 Size: 6067 AclI AA'CG_TT Cuts at: 1563 4194 4567 1563 Size: 2631 373 3063 Fragments arranged by size: 3063 2631 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2622 2674 4130 5514 5782 1632 Size: 990 52 1456 1384 268 1917 Fragments arranged by size: 1917 1456 1384 990 268 52 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5870 AflIII A'CryG_T Cuts at: 1203 4966 5943 1203 Size: 3763 977 1327 Fragments arranged by size: 3763 1327 977 AgeI A'CCGG_T Cuts at: 4898 4898 Size: 6067 AhdI GACnn_n'nnGTC Cuts at: 4796 4796 Size: 6067 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6067 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6067 AlwNI CAG_nnn'CTG Cuts at: 3810 5382 3810 Size: 1572 4495 ApaI G_GGCC'C Cuts at: 2217 2217 Size: 6067 ApaLI G'TGCA_C Cuts at: 3242 4124 5280 3242 Size: 882 1156 4029 Fragments arranged by size: 4029 1156 882 ApoI r'AATT_y Cuts at: 1493 2048 2169 2802 3376 3602 3754 5863 1493 Size: 555 121 633 574 226 152 2109 1697 Fragments arranged by size: 2109 1697 633 574 555 226 152 121 AseI AT'TA_AT Cuts at: 160 1032 4621 160 Size: 872 3589 1606 Fragments arranged by size: 3589 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 AvaI C'yCGr_G Cuts at: 1870 1943 2337 3392 1870 Size: 73 394 1055 4545 Fragments arranged by size: 4545 1055 394 73 BamHI G'GATC_C Cuts at: 3173 3397 3764 3173 Size: 224 367 5476 Fragments arranged by size: 5476 367 224 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2917 3388 4843 618 Size: 325 217 627 1130 471 1455 1842 Fragments arranged by size: 1842 1455 1130 627 471 325 217 BanII G_rGCy'C Cuts at: 1391 2217 2814 2886 2972 3386 1391 Size: 826 597 72 86 414 4072 Fragments arranged by size: 4072 826 597 414 86 72 BbeI G_GCGC'C Cuts at: 2921 2921 Size: 6067 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5096 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2184 2218 2226 2260 2986 3020 4258 Size: 34 247 34 8 34 726 34 1238 Cuts at: 4258 4292 1903 Size: 34 3678 Fragments arranged by size: 3678 1238 726 247 34 34 34 34 34 8 BciVI GTATCCnnnnn_n' Cuts at: 3959 5169 3959 Size: 1210 4857 BclI T'GATC_A Cuts at: 1548 1548 Size: 6067 BfrBI ATG'CAT Cuts at: 3138 3138 Size: 6067 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2249 4678 136 Size: 107 122 71 631 605 577 2429 1525 Fragments arranged by size: 2429 1525 631 605 577 122 107 71 BglII A'GATC_T Cuts at: 6062 6062 Size: 6067 BlpI GC'TnA_GC Cuts at: 2604 3484 2604 Size: 880 5187 Bme1580I G_kGCm'C Cuts at: 1790 2217 2410 3246 4128 5284 1790 Size: 427 193 836 882 1156 2573 Fragments arranged by size: 2573 1156 882 836 427 193 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6067 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3798 4756 442 Size: 416 53 83 399 24 2381 958 1753 Fragments arranged by size: 2381 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6067 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2619 2619 Size: 6067 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2047 2615 2828 2956 4727 1239 Size: 808 568 213 128 1771 2579 Fragments arranged by size: 2579 1771 808 568 213 128 Bpu10I CC'TnA_GC Cuts at: 2594 2676 2966 3354 2594 Size: 82 290 388 5307 Fragments arranged by size: 5307 388 290 82 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4192 5057 5355 4192 Size: 865 298 4904 Fragments arranged by size: 4904 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2572 4730 882 Size: 617 1073 2158 2219 Fragments arranged by size: 2219 2158 1073 617 BsaAI yAC'GTr Cuts at: 493 1204 3858 493 Size: 711 2654 2702 Fragments arranged by size: 2702 2654 711 BsaBI GATnn'nnATC Cuts at: 6061 6061 Size: 6067 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2918 3196 3874 4256 Size: 53 83 186 936 1385 278 678 382 Cuts at: 4256 4891 275 Size: 635 1451 Fragments arranged by size: 1451 1385 936 678 635 382 278 186 83 53 BsaWI w'CCGG_w Cuts at: 3201 4500 4898 5172 5319 5730 3201 Size: 1299 398 274 147 411 3538 Fragments arranged by size: 3538 1299 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2905 2935 2984 3014 1178 Size: 30 165 30 1502 30 49 30 4231 Fragments arranged by size: 4231 1502 165 49 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2876 1186 Size: 1690 4377 BseYI C'CCAG_C Cuts at: 2210 2216 2270 5270 5766 2210 Size: 6 54 3000 496 2511 Fragments arranged by size: 3000 2511 496 54 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2207 2633 2953 3136 1163 Size: 573 471 426 320 183 4094 Fragments arranged by size: 4094 573 471 426 320 183 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3517 4278 5306 1540 Size: 275 186 1516 761 1028 2301 Fragments arranged by size: 2301 1516 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2600 2814 2972 3246 4128 4213 5284 2600 Size: 214 158 274 882 85 1071 3383 Fragments arranged by size: 3383 1071 882 274 214 158 85 BsmI GAATG_Cn' Cuts at: 3578 3671 3578 Size: 93 5974 BsmBI CGTCTCn'nnnn_ Cuts at: 3203 3203 Size: 6067 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 2410 2600 2814 2886 2972 3246 Size: 399 427 193 190 214 72 86 274 Cuts at: 3246 3386 4128 4213 5284 1391 Size: 140 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 427 399 274 214 193 190 140 86 85 72 BspEI T'CCGG_A Cuts at: 5730 5730 Size: 6067 BspHI T'CATG_A Cuts at: 2310 3956 5686 2310 Size: 1646 1730 2691 Fragments arranged by size: 2691 1730 1646 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3422 844 Size: 2578 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2961 3954 1394 Size: 429 1138 993 3507 Fragments arranged by size: 3507 1138 993 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4562 4736 65 Size: 1183 3314 174 1396 Fragments arranged by size: 3314 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3089 4711 4898 1700 Size: 110 1279 1622 187 2869 Fragments arranged by size: 2869 1622 1279 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6067 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6067 BssSI C'ACGA_G Cuts at: 2609 4127 5139 2609 Size: 1518 1012 3537 Fragments arranged by size: 3537 1518 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6067 BstBI TT'CG_AA Cuts at: 4871 4871 Size: 6067 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6067 BstXI CCAn_nnnn'nTGG Cuts at: 2270 2270 Size: 6067 BstYI r'GATC_y Cuts at: 1972 3173 3397 3764 4149 4166 5618 5704 5716 Size: 1201 224 367 385 17 1452 86 12 Cuts at: 5716 6062 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1201 385 367 346 224 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2487 3177 513 Size: 552 939 483 690 3403 Fragments arranged by size: 3403 939 690 552 483 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5328 Fragments arranged by size: 5328 683 56 BtsI GCAGTG_nn' Cuts at: 2227 3244 3583 4377 4397 2227 Size: 1017 339 794 20 3897 Fragments arranged by size: 3897 1017 794 339 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6067 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6032 DraI TTT'AAA Cuts at: 3371 3371 Size: 6067 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5074 809 Size: 507 3758 1802 Fragments arranged by size: 3758 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3514 4403 6032 Size: 54 1425 50 275 18 1684 889 1629 Cuts at: 6032 8 Size: 43 Fragments arranged by size: 1684 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3514 1537 Size: 275 1702 4090 Fragments arranged by size: 4090 1702 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3997 1353 Size: 2644 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4654 5028 5174 387 Size: 1064 138 3065 374 146 1280 Fragments arranged by size: 3065 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2812 2970 2812 Size: 158 5909 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2047 2615 2622 2674 2828 2956 4130 Size: 393 415 568 7 52 154 128 1174 Cuts at: 4130 4727 5514 5782 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1174 787 597 568 415 393 268 154 128 52 7 EcoNI CCTnn'n_nnAGG Cuts at: 2296 2296 Size: 6067 EcoO109I rG'GnC_Cy Cuts at: 2067 2626 2067 Size: 559 5508 EcoRI G'AATT_C Cuts at: 3376 3754 3376 Size: 378 5689 FspI TGC'GCA Cuts at: 4573 4573 Size: 6067 HaeII r_GCGC'y Cuts at: 1678 2486 2921 5214 1678 Size: 808 435 2293 2531 Fragments arranged by size: 2531 2293 808 435 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2090 2122 2905 2937 2984 Size: 32 135 32 518 32 783 32 47 Cuts at: 2984 3016 3265 3297 4708 4740 4782 4814 1373 Size: 32 249 32 1411 32 42 32 2626 Fragments arranged by size: 2626 1411 783 518 249 135 47 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3411 3657 669 Size: 988 281 1473 246 3079 Fragments arranged by size: 3079 1473 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6067 HpaI GTT'AAC Cuts at: 3657 3657 Size: 6067 KasI G'GCGC_C Cuts at: 2917 2917 Size: 6067 KpnI G_GTAC'C Cuts at: 3392 3392 Size: 6067 MfeI C'AATT_G Cuts at: 2540 3666 2540 Size: 1126 4941 MluI A'CGCG_T Cuts at: 5943 5943 Size: 6067 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2673 5181 5365 1305 Size: 1368 2508 184 2007 Fragments arranged by size: 2508 2007 1368 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4245 Fragments arranged by size: 4245 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2847 4025 4384 4543 518 Size: 648 1681 1178 359 159 2042 Fragments arranged by size: 2042 1681 1178 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2191 2270 3483 4160 5308 5553 1606 Size: 585 79 1213 677 1148 245 2120 Fragments arranged by size: 2120 1213 1148 677 585 245 79 NaeI GCC'GGC Cuts at: 3091 3091 Size: 6067 NarI GG'CG_CC Cuts at: 2918 2918 Size: 6067 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4576 Fragments arranged by size: 4576 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6067 NgoMIV G'CCGG_C Cuts at: 3089 3089 Size: 6067 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6067 NotI GC'GGCC_GC Cuts at: 3514 3514 Size: 6067 NsiI A_TGCA'T Cuts at: 3140 3140 Size: 6067 NspI r_CATG'y Cuts at: 1132 1229 3425 4970 1132 Size: 97 2196 1545 2229 Fragments arranged by size: 2229 2196 1545 97 PasI CC'CwG_GG Cuts at: 1348 2155 1348 Size: 807 5260 PciI A'CATG_T Cuts at: 4966 4966 Size: 6067 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6067 PmeI GTTT'AAAC Cuts at: 3371 3371 Size: 6067 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4136 4168 5649 5681 4136 Size: 32 1481 32 4522 Fragments arranged by size: 4522 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2067 2067 Size: 6067 PshAI GACnn'nnGTC Cuts at: 1613 3195 1613 Size: 1582 4485 PsiI TTA'TAA Cuts at: 3637 3637 Size: 6067 PspOMI G'GGCC_C Cuts at: 2213 2213 Size: 6067 PstI C_TGCA'G Cuts at: 830 2482 2516 3081 3419 830 Size: 1652 34 565 338 3478 Fragments arranged by size: 3478 1652 565 338 34 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6067 PvuII CAG'CTG Cuts at: 2191 2270 2191 Size: 79 5988 SacI G_AGCT'C Cuts at: 2814 2972 2814 Size: 158 5909 SalI G'TCGA_C Cuts at: 1936 3409 1936 Size: 1473 4594 SbfI CC_TGCA'GG Cuts at: 3419 3419 Size: 6067 ScaI AGT'ACT Cuts at: 4315 6027 4315 Size: 1712 4355 SfcI C'TryA_G Cuts at: 826 1045 2293 2478 2512 2786 3077 3415 4550 Size: 219 1248 185 34 274 291 338 1135 Cuts at: 4550 5231 5422 826 Size: 681 191 1471 Fragments arranged by size: 1471 1248 1135 681 338 291 274 219 191 185 34 SfoI GGC'GCC Cuts at: 2919 2919 Size: 6067 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6067 SmaI CCC'GGG Cuts at: 1872 3394 1872 Size: 1522 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2337 4171 5072 5334 820 Size: 197 926 394 1834 901 262 1553 Fragments arranged by size: 1834 1553 926 901 394 262 197 SnaBI TAC'GTA Cuts at: 493 3858 493 Size: 3365 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6067 SphI G_CATG'C Cuts at: 1132 3425 1132 Size: 2293 3774 SspI AAT'ATT Cuts at: 5 52 3991 5 Size: 47 3939 2081 Fragments arranged by size: 3939 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6067 StyI C'CwwG_G Cuts at: 513 1065 2004 2148 2988 3009 3506 513 Size: 552 939 144 840 21 497 3074 Fragments arranged by size: 3074 939 840 552 497 144 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2043 4094 4111 4264 4449 4894 325 Size: 1255 463 2051 17 153 185 445 1498 Fragments arranged by size: 2051 1498 1255 463 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4313 6025 96 Size: 274 80 33 51 1443 2336 1712 138 Fragments arranged by size: 2336 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6027 6059 6027 Size: 32 6035 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6067 XbaI T'CTAG_A Cuts at: 3403 3403 Size: 6067 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3140 3140 Size: 6067 XhoI C'TCGA_G Cuts at: 1943 2337 1943 Size: 394 5673 XmaI C'CCGG_G Cuts at: 1870 3392 1870 Size: 1522 4545 XmnI GAAnn'nnTTC Cuts at: 4196 4196 Size: 6067 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3197 3875 276 Size: 53 83 186 936 1663 678 2468 Fragments arranged by size: 2468 1663 936 678 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI