(Linear) (Six-base) MAPSORT of: KIEE5574.seq Check: 6730 from: 1 to: 1362 KIEE5574.orf With 182 enzymes: SgfI * January 29, 2014 12:06 .. AatII G_ACGT'C Cuts at: 0 1194 1362 Size: 1194 168 AccI GT'mk_AC Cuts at: 0 1262 1362 Size: 1262 100 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 617 669 1362 Size: 617 52 693 Fragments arranged by size: 693 617 52 ApaI G_GGCC'C Cuts at: 0 212 1362 Size: 212 1150 ApaLI G'TGCA_C Cuts at: 0 1237 1362 Size: 1237 125 ApoI r'AATT_y Cuts at: 0 43 164 797 1362 Size: 43 121 633 565 Fragments arranged by size: 633 565 121 43 AvaI C'yCGr_G Cuts at: 0 332 1362 Size: 332 1030 BamHI G'GATC_C Cuts at: 0 1168 1362 Size: 1168 194 BanI G'GyrC_C Cuts at: 0 912 1362 Size: 912 450 BanII G_rGCy'C Cuts at: 0 212 809 881 967 1362 Size: 212 597 72 86 395 Fragments arranged by size: 597 395 212 86 72 BbeI G_GCGC'C Cuts at: 0 916 1362 Size: 916 446 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 179 213 221 255 981 1015 1362 Size: 179 34 8 34 726 34 347 Fragments arranged by size: 726 347 179 34 34 34 8 BfrBI ATG'CAT Cuts at: 0 1133 1362 Size: 1133 229 BglI GCCn_nnn'nGGC Cuts at: 0 244 1362 Size: 244 1118 BlpI GC'TnA_GC Cuts at: 0 599 1362 Size: 599 763 Bme1580I G_kGCm'C Cuts at: 0 212 405 1241 1362 Size: 212 193 836 121 Fragments arranged by size: 836 212 193 121 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 614 1362 Size: 614 748 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 42 610 823 951 1362 Size: 42 568 213 128 411 Fragments arranged by size: 568 411 213 128 42 Bpu10I CC'TnA_GC Cuts at: 0 589 671 961 1349 1362 Size: 589 82 290 388 13 Fragments arranged by size: 589 388 290 82 13 BsaI GGTCTCn'nnnn_ Cuts at: 0 567 1362 Size: 567 795 BsaHI Gr'CG_yC Cuts at: 0 913 1191 1362 Size: 913 278 171 Fragments arranged by size: 913 278 171 BsaWI w'CCGG_w Cuts at: 0 1196 1362 Size: 1196 166 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 900 930 979 1009 1362 Size: 900 30 49 30 353 Fragments arranged by size: 900 353 49 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 871 1362 Size: 871 491 BseYI C'CCAG_C Cuts at: 0 205 211 265 1362 Size: 205 6 54 1097 Fragments arranged by size: 1097 205 54 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 628 948 1131 1362 Size: 202 426 320 183 231 Fragments arranged by size: 426 320 231 202 183 BsiHKAI G_wGCw'C Cuts at: 0 595 809 967 1241 1362 Size: 595 214 158 274 121 Fragments arranged by size: 595 274 214 158 121 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1198 1362 Size: 1198 164 Bsp1286I G_dGCh'C Cuts at: 0 212 405 595 809 881 967 1241 1362 Size: 212 193 190 214 72 86 274 121 Fragments arranged by size: 274 214 212 193 190 121 86 72 BspHI T'CATG_A Cuts at: 0 305 1362 Size: 305 1057 BsrBI CCG'CTC Cuts at: 0 956 1362 Size: 956 406 BsrFI r'CCGG_y Cuts at: 0 1084 1362 Size: 1084 278 BssSI C'ACGA_G Cuts at: 0 604 1362 Size: 604 758 BstXI CCAn_nnnn'nTGG Cuts at: 0 265 1362 Size: 265 1097 BstYI r'GATC_y Cuts at: 0 1168 1362 Size: 1168 194 BtgI C'CryG_G Cuts at: 0 482 1172 1362 Size: 482 690 190 Fragments arranged by size: 690 482 190 BtsI GCAGTG_nn' Cuts at: 0 222 1239 1362 Size: 222 1017 123 Fragments arranged by size: 1017 222 123 EcoICRI GAG'CTC Cuts at: 0 807 965 1362 Size: 807 158 397 Fragments arranged by size: 807 397 158 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 42 610 617 669 823 951 1362 Size: 42 568 7 52 154 128 411 Fragments arranged by size: 568 411 154 128 52 42 7 EcoNI CCTnn'n_nnAGG Cuts at: 0 291 1362 Size: 291 1071 EcoO109I rG'GnC_Cy Cuts at: 0 62 621 1362 Size: 62 559 741 Fragments arranged by size: 741 559 62 HaeII r_GCGC'y Cuts at: 0 481 916 1362 Size: 481 435 446 Fragments arranged by size: 481 446 435 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 85 117 900 932 979 1011 1260 1292 Size: 85 32 783 32 47 32 249 32 Cuts at: 1292 1362 Size: 70 Fragments arranged by size: 783 249 85 70 47 32 32 32 32 KasI G'GCGC_C Cuts at: 0 912 1362 Size: 912 450 MfeI C'AATT_G Cuts at: 0 535 1362 Size: 535 827 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 668 1362 Size: 668 694 MslI CAynn'nnrTG Cuts at: 0 842 1362 Size: 842 520 MspA1I CmG'CkG Cuts at: 0 186 265 1362 Size: 186 79 1097 Fragments arranged by size: 1097 186 79 NaeI GCC'GGC Cuts at: 0 1086 1362 Size: 1086 276 NarI GG'CG_CC Cuts at: 0 913 1362 Size: 913 449 NgoMIV G'CCGG_C Cuts at: 0 1084 1362 Size: 1084 278 NsiI A_TGCA'T Cuts at: 0 1135 1362 Size: 1135 227 PasI CC'CwG_GG Cuts at: 0 150 1362 Size: 150 1212 PpuMI rG'GwC_Cy Cuts at: 0 62 1362 Size: 62 1300 PshAI GACnn'nnGTC Cuts at: 0 1190 1362 Size: 1190 172 PspOMI G'GGCC_C Cuts at: 0 208 1362 Size: 208 1154 PstI C_TGCA'G Cuts at: 0 477 511 1076 1362 Size: 477 34 565 286 Fragments arranged by size: 565 477 286 34 PvuII CAG'CTG Cuts at: 0 186 265 1362 Size: 186 79 1097 Fragments arranged by size: 1097 186 79 SacI G_AGCT'C Cuts at: 0 809 967 1362 Size: 809 158 395 Fragments arranged by size: 809 395 158 SfcI C'TryA_G Cuts at: 0 288 473 507 781 1072 1362 Size: 288 185 34 274 291 290 Fragments arranged by size: 291 290 288 274 185 34 SfoI GGC'GCC Cuts at: 0 914 1362 Size: 914 448 SmlI C'TyrA_G Cuts at: 0 332 1362 Size: 332 1030 StyI C'CwwG_G Cuts at: 0 143 983 1004 1362 Size: 143 840 21 358 Fragments arranged by size: 840 358 143 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 38 1362 Size: 38 1324 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1135 1362 Size: 1135 227 XhoI C'TCGA_G Cuts at: 0 332 1362 Size: 332 1030 ZraI GAC'GTC Cuts at: 0 1192 1362 Size: 1192 170 Enzymes that do cut: AatII AccI AcuI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BcgI BfrBI BglI BlpI Bme1580I BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BsrBI BsrFI BssSI BstXI BstYI BtgI BtsI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MfeI MmeI MslI MspA1I NaeI NarI NgoMIV NsiI PasI PpuMI PshAI PspOMI PstI PvuII SacI SfcI SfoI SmlI StyI TaqII XcmI XhoI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI AscI AseI AsiSI AvrII BaeI BbsI BbvCI BciVI BclI BglII BmgBI BmrI BmtI BpuEI BsaAI BsaBI BsiEI BsiWI BsmI BspEI BspMI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MluI MscI NcoI NdeI NheI NotI NruI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XmaI XmnI