Restriction map for
pFN21AE5825
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# NO CUT ENZYMES ( 86 enzymes) #
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Acc65I AccII AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BamHI BanII
BbsI BbvCI BfrI BlnI BlpI
Bpu1102I BpuAI BsaBI BsaI BsiHKAI
BsiWI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstUI BstZ17I CelII
Cfr10I CpoI Eam1105I Eco47III FseI
FspI HgaI KpnI KspI MamI
MluI MvnI NaeI NgoMIV NheI
NotI NruI NspI PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PvuI RsrII SacI SacII SalI
SbfI SfiI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I Van91I XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 142 GACGTC GACGT^C
AccI 3233 GTAGAC GT^[AC][GT]AC
AclI 442 AACGTT AA^CGTT
AseI 2701 ATTAAT AT^TAAT
AsnI 2701 ATTAAT AT^TAAT
AvaI 825 CTCGGG C^[CT]CG[GA]G
BbeI 28 GGCGCC GGCGC^C
BbrPI 1312 CACGTG CAC^GTG
BcgI 3459 GCAGCACATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1891 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2871 AGATCT A^GATCT
BsiEI 1631 CGGCCG CG[GA][CT]^CG
BsoBI 825 CTCGGG C^[CT]CG[GA]G
Bsp1407I 3351 TGTACA T^GTACA
BspDI 2885 ATCGAT AT^CGAT
BsrGI 3351 TGTACA T^GTACA
BstAPI 3322 GCAAATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 205 TTCGAA TT^CGAA
CfoI 29 GCGC GCG^C
ClaI 2885 ATCGAT AT^CGAT
DraI 2724 TTTAAA TTT^AAA
DrdI 987 GACAGAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1631 CGGCCG C^GGCCG
EclXI 1631 CGGCCG C^GGCCG
Eco52I 1631 CGGCCG C^GGCCG
EcoRI 217 GAATTC G^AATTC
EcoRV 2851 GATATC GAT^ATC
EcoT22I 3277 ATGCAT ATGCA^T
FauI 32 CCCGC CCCGC(4/6)
HaeII 28 GGCGCC [GA]GCGC^[CT]
HhaI 29 GCGC GCG^C
HinP1I 29 GCGC G^CGC
HpaI 342 GTTAAC GTT^AAC
KasI 28 GGCGCC G^GCGCC
MfeI 2367 CAATTG C^AATTG
MspA1I 577 CAGCTG C[AC]G^C[GT]G
MunI 2367 CAATTG C^AATTG
NarI 28 GGCGCC GG^CGCC
NsiI 3277 ATGCAT ATGCA^T
NspV 205 TTCGAA TT^CGAA
PmaCI 1312 CACGTG CAC^GTG
PmlI 1312 CACGTG CAC^GTG
PshBI 2701 ATTAAT AT^TAAT
Psp1406I 442 AACGTT AA^CGTT
PvuII 577 CAGCTG CAG^CTG
SapI 3211 GAAGAGC GCTCTTC(1/4)
ScaI 1451 AGTACT AGT^ACT
SfoI 28 GGCGCC GGC^GCC
SfuI 205 TTCGAA TT^CGAA
SmlI 1470 CTCAAG C^T[CT][GA]AG
SspBI 3351 TGTACA T^GTACA
XbaI 1987 TCTAGA T^CTAGA
XcmI 1388 CCATTGTGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcyI 28 GGCGCC G[GA]^CG[CT]C
142 GACGTC
AocI 1208 CCTCAGG CC^T[ACGT]AGG
1530 CCTAAGG
BclI 3303 TGATCA T^GATCA
3395 TGATCA
BcnI 1818 CCGGG CC^[GC]GG
2748 CCCGG
BsaAI 1312 CACGTG [CT]AC^GT[GA]
1681 TACGTG
BsaHI 28 GGCGCC G[GA]^CG[CT]C
142 GACGTC
BsgI 1455 CTGCAC GTGCAG(16/14)
2966 GTGCAG
BsmBI 881 CGTCTC CGTCTC(1/5)
2424 CGTCTC
BsmI 75 GAATGC GAATGC(1/-1)
1991 GAATGC
BspMI 8 ACCTGC ACCTGC(4/8)
1411 ACCTGC
BsrDI 3438 CATTGC GCAATG(2/0)
2051 GCAATG
BstXI 889 CCATATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2617 CCATACAACTGG
Bsu36I 1208 CCTCAGG CC^T[ACGT]AGG
1530 CCTAAGG
DraIII 977 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3108 CACTCAGTG
Eco81I 1208 CCTCAGG CC^T[ACGT]AGG
1530 CCTAAGG
FbaI 3303 TGATCA T^GATCA
3395 TGATCA
Hin1I 28 GGCGCC G[GA]^CG[CT]C
142 GACGTC
HindIII 134 AAGCTT A^AGCTT
2611 AAGCTT
NciI 1818 CCGGG CC^[GC]GG
2748 CCCGG
NcoI 953 CCATGG C^CATGG
1826 CCATGG
NdeI 1955 CATATG CA^TATG
2918 CATATG
PleI 2797 GACTC GAGTC(4/5)
407 GAGTC
PpuMI 1623 GGGTCCT [GA]G^G[AT]CC[CT]
3387 AGGTCCC
SexAI 1042 ACCTGGT A^CC[AT]GGT
1514 ACCAGGT
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
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AccIII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII BalI
BanI BbvI BciVI BfaI BmyI
BpmI BsaJI BsaWI BseAI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspEI BspHI BsrI BssKI BstEII
BstF5I BstNI BstPI BstYI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoO65I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII