(Linear) (Six-base) MAPSORT of: KIEE5825.seq Check: 6490 from: 1 to: 3648 KIEE5825.orf With 182 enzymes: SgfI * December 5, 2014 13:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 17 3648 Size: 17 3631 AatII G_ACGT'C Cuts at: 0 146 3648 Size: 146 3502 AccI GT'mk_AC Cuts at: 0 3234 3648 Size: 3234 414 AclI AA'CG_TT Cuts at: 0 443 3648 Size: 443 3205 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 122 1183 1256 1544 2195 2284 2803 2910 Size: 122 1061 73 288 651 89 519 107 Cuts at: 2910 3648 Size: 738 Fragments arranged by size: 1061 738 651 519 288 122 107 89 73 AleI CACnn'nnGTG Cuts at: 0 454 1316 1390 1462 1915 3407 3648 Size: 454 862 74 72 453 1492 241 Fragments arranged by size: 1492 862 454 453 241 74 72 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 674 3648 Size: 674 2974 AlwNI CAG_nnn'CTG Cuts at: 0 160 919 1567 1756 2110 2314 3648 Size: 160 759 648 189 354 204 1334 Fragments arranged by size: 1334 759 648 354 204 189 160 ApoI r'AATT_y Cuts at: 0 208 217 818 934 1147 2809 3648 Size: 208 9 601 116 213 1662 839 Fragments arranged by size: 1662 839 601 213 208 116 9 AseI AT'TA_AT Cuts at: 0 2702 3648 Size: 2702 946 AvaI C'yCGr_G Cuts at: 0 825 3648 Size: 825 2823 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2564 2597 3648 Size: 2564 33 1051 Fragments arranged by size: 2564 1051 33 BanI G'GyrC_C Cuts at: 0 5 28 1504 3648 Size: 5 23 1476 2144 Fragments arranged by size: 2144 1476 23 5 BbeI G_GCGC'C Cuts at: 0 32 3648 Size: 32 3616 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3448 3482 3648 Size: 3448 34 166 Fragments arranged by size: 3448 166 34 BciVI GTATCCnnnnn_n' Cuts at: 0 14 2992 3349 3648 Size: 14 2978 357 299 Fragments arranged by size: 2978 357 299 14 BclI T'GATC_A Cuts at: 0 3303 3395 3648 Size: 3303 92 253 Fragments arranged by size: 3303 253 92 BfrBI ATG'CAT Cuts at: 0 3279 3648 Size: 3279 369 BglI GCCn_nnn'nGGC Cuts at: 0 1897 3648 Size: 1897 1751 BglII A'GATC_T Cuts at: 0 2871 3648 Size: 2871 777 Bme1580I G_kGCm'C Cuts at: 0 8 1509 3317 3648 Size: 8 1501 1808 331 Fragments arranged by size: 1808 1501 331 8 BmgBI CAC'GTC Cuts at: 0 1835 3648 Size: 1835 1813 BmrI ACTGGGnnnn_n' Cuts at: 0 480 726 1503 1888 2396 3234 3648 Size: 480 246 777 385 508 838 414 Fragments arranged by size: 838 777 508 480 414 385 246 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2066 2265 2325 3648 Size: 2066 199 60 1323 Fragments arranged by size: 2066 1323 199 60 Bpu10I CC'TnA_GC Cuts at: 0 2485 3648 Size: 2485 1163 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1455 3648 Size: 1455 2193 BsaAI yAC'GTr Cuts at: 0 1314 1683 3648 Size: 1314 369 1965 Fragments arranged by size: 1965 1314 369 BsaHI Gr'CG_yC Cuts at: 0 29 143 3648 Size: 29 114 3505 Fragments arranged by size: 3505 114 29 BsaWI w'CCGG_w Cuts at: 0 488 841 1902 3242 3521 3648 Size: 488 353 1061 1340 279 127 Fragments arranged by size: 1340 1061 488 353 279 127 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 219 249 3648 Size: 219 30 3399 Fragments arranged by size: 3399 219 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 227 2842 3559 3648 Size: 227 2615 717 89 Fragments arranged by size: 2615 717 227 89 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1440 2987 3648 Size: 1440 1547 661 Fragments arranged by size: 1547 1440 661 BsiEI CG_ry'CG Cuts at: 0 1634 3648 Size: 1634 2014 BsmI GAATG_Cn' Cuts at: 0 81 1997 3648 Size: 81 1916 1651 Fragments arranged by size: 1916 1651 81 BsmBI CGTCTCn'nnnn_ Cuts at: 0 887 2430 3648 Size: 887 1543 1218 Fragments arranged by size: 1543 1218 887 Bsp1286I G_dGCh'C Cuts at: 0 8 1509 3317 3648 Size: 8 1501 1808 331 Fragments arranged by size: 1808 1501 331 8 BspEI T'CCGG_A Cuts at: 0 488 1902 3242 3648 Size: 488 1414 1340 406 Fragments arranged by size: 1414 1340 488 406 BspHI T'CATG_A Cuts at: 0 755 2057 3474 3648 Size: 755 1302 1417 174 Fragments arranged by size: 1417 1302 755 174 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 17 1420 3648 Size: 17 1403 2228 Fragments arranged by size: 2228 1403 17 BsrDI GCAATG_nn' Cuts at: 0 2058 3437 3648 Size: 2058 1379 211 Fragments arranged by size: 2058 1379 211 BsrGI T'GTAC_A Cuts at: 0 3351 3648 Size: 3351 297 BstAPI GCAn_nnn'nTGC Cuts at: 0 3328 3648 Size: 3328 320 BstBI TT'CG_AA Cuts at: 0 206 3648 Size: 206 3442 BstEII G'GTnAC_C Cuts at: 0 312 1046 1821 3648 Size: 312 734 775 1827 Fragments arranged by size: 1827 775 734 312 BstXI CCAn_nnnn'nTGG Cuts at: 0 896 2624 3648 Size: 896 1728 1024 Fragments arranged by size: 1728 1024 896 BstYI r'GATC_y Cuts at: 0 1213 1563 1842 2871 3648 Size: 1213 350 279 1029 777 Fragments arranged by size: 1213 1029 777 350 279 Bsu36I CC'TnA_GG Cuts at: 0 1209 1531 3648 Size: 1209 322 2117 Fragments arranged by size: 2117 1209 322 BtgI C'CryG_G Cuts at: 0 14 953 1634 1826 3648 Size: 14 939 681 192 1822 Fragments arranged by size: 1822 939 681 192 14 BtsI GCAGTG_nn' Cuts at: 0 870 1064 1340 2198 2357 3037 3290 3402 Size: 870 194 276 858 159 680 253 112 Cuts at: 3402 3414 3648 Size: 12 234 Fragments arranged by size: 870 858 680 276 253 234 194 159 112 12 ClaI AT'CG_AT Cuts at: 0 2886 3648 Size: 2886 762 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1053 1088 3565 3600 3648 Size: 1053 35 2477 35 48 Fragments arranged by size: 2477 1053 48 35 35 DraI TTT'AAA Cuts at: 0 2726 3648 Size: 2726 922 DraIII CAC_nnn'GTG Cuts at: 0 982 3113 3648 Size: 982 2131 535 Fragments arranged by size: 2131 982 535 DrdI GACnn_nn'nnGTC Cuts at: 0 993 3648 Size: 993 2655 EaeI y'GGCC_r Cuts at: 0 1631 2477 2789 3151 3648 Size: 1631 846 312 362 497 Fragments arranged by size: 1631 846 497 362 312 EagI C'GGCC_G Cuts at: 0 1631 3648 Size: 1631 2017 EarI CTCTTCn'nnn_ Cuts at: 0 796 1097 2191 2228 2725 2822 2951 3206 Size: 796 301 1094 37 497 97 129 255 Cuts at: 3206 3648 Size: 442 Fragments arranged by size: 1094 796 497 442 301 255 129 97 37 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2137 3648 Size: 2137 1511 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 122 1183 1256 1544 2066 2195 2265 2284 Size: 122 1061 73 288 522 129 70 19 Cuts at: 2284 2325 2803 2910 3648 Size: 41 478 107 738 Fragments arranged by size: 1061 738 522 478 288 129 122 107 73 70 41 19 EcoNI CCTnn'n_nnAGG Cuts at: 0 401 1661 2598 3648 Size: 401 1260 937 1050 Fragments arranged by size: 1260 1050 937 401 EcoO109I rG'GnC_Cy Cuts at: 0 1540 1624 3388 3648 Size: 1540 84 1764 260 Fragments arranged by size: 1764 1540 260 84 EcoRI G'AATT_C Cuts at: 0 217 3648 Size: 217 3431 EcoRV GAT'ATC Cuts at: 0 2853 3648 Size: 2853 795 HaeII r_GCGC'y Cuts at: 0 32 3648 Size: 32 3616 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 892 924 2667 2699 2837 2869 3372 3404 Size: 892 32 1743 32 138 32 503 32 Cuts at: 3404 3648 Size: 244 Fragments arranged by size: 1743 892 503 244 138 32 32 32 32 HincII GTy'rAC Cuts at: 0 344 998 2269 3486 3648 Size: 344 654 1271 1217 162 Fragments arranged by size: 1271 1217 654 344 162 HindIII A'AGCT_T Cuts at: 0 134 2611 3648 Size: 134 2477 1037 Fragments arranged by size: 2477 1037 134 HpaI GTT'AAC Cuts at: 0 344 3648 Size: 344 3304 KasI G'GCGC_C Cuts at: 0 28 3648 Size: 28 3620 MfeI C'AATT_G Cuts at: 0 2367 3648 Size: 2367 1281 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1930 3387 3648 Size: 1930 1457 261 Fragments arranged by size: 1930 1457 261 MscI TGG'CCA Cuts at: 0 2479 2791 3153 3648 Size: 2479 312 362 495 Fragments arranged by size: 2479 495 362 312 MslI CAynn'nnrTG Cuts at: 0 454 894 1316 1390 1462 1725 1915 3190 Size: 454 440 422 74 72 263 190 1275 Cuts at: 3190 3407 3569 3648 Size: 217 162 79 Fragments arranged by size: 1275 454 440 422 263 217 190 162 79 74 72 MspA1I CmG'CkG Cuts at: 0 579 3648 Size: 579 3069 NarI GG'CG_CC Cuts at: 0 29 3648 Size: 29 3619 NcoI C'CATG_G Cuts at: 0 953 1826 3648 Size: 953 873 1822 Fragments arranged by size: 1822 953 873 NdeI CA'TA_TG Cuts at: 0 1956 2919 3648 Size: 1956 963 729 Fragments arranged by size: 1956 963 729 NsiI A_TGCA'T Cuts at: 0 3281 3648 Size: 3281 367 PasI CC'CwG_GG Cuts at: 0 3030 3648 Size: 3030 618 PfoI T'CCnGG_A Cuts at: 0 1354 2994 3648 Size: 1354 1640 654 Fragments arranged by size: 1640 1354 654 PmlI CAC'GTG Cuts at: 0 1314 3648 Size: 1314 2334 PpuMI rG'GwC_Cy Cuts at: 0 1624 3388 3648 Size: 1624 1764 260 Fragments arranged by size: 1764 1624 260 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 451 483 3648 Size: 451 32 3165 Fragments arranged by size: 3165 451 32 PstI C_TGCA'G Cuts at: 0 915 2549 2871 3333 3347 3409 3648 Size: 915 1634 322 462 14 62 239 Fragments arranged by size: 1634 915 462 322 239 62 14 PvuII CAG'CTG Cuts at: 0 579 3648 Size: 579 3069 SapI GCTCTTCn'nnn_ Cuts at: 0 3206 3648 Size: 3206 442 ScaI AGT'ACT Cuts at: 0 1453 3648 Size: 1453 2195 SexAI A'CCwGG_T Cuts at: 0 1042 1514 3648 Size: 1042 472 2134 Fragments arranged by size: 2134 1042 472 SfcI C'TryA_G Cuts at: 0 80 911 2545 2867 3170 3231 3329 3343 Size: 80 831 1634 322 303 61 98 14 Cuts at: 3343 3405 3648 Size: 62 243 Fragments arranged by size: 1634 831 322 303 243 98 80 62 61 14 SfoI GGC'GCC Cuts at: 0 30 3648 Size: 30 3618 SmlI C'TyrA_G Cuts at: 0 1470 3648 Size: 1470 2178 SspI AAT'ATT Cuts at: 0 681 1120 1924 3648 Size: 681 439 804 1724 Fragments arranged by size: 1724 804 681 439 StyI C'CwwG_G Cuts at: 0 953 1826 2707 3648 Size: 953 873 881 941 Fragments arranged by size: 953 941 881 873 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1068 1077 1312 2190 2217 3043 3648 Size: 1068 9 235 878 27 826 605 Fragments arranged by size: 1068 878 826 605 235 27 9 TatI w'GTAC_w Cuts at: 0 620 973 1451 2116 3351 3551 3648 Size: 620 353 478 665 1235 200 97 Fragments arranged by size: 1235 665 620 478 353 200 97 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 219 251 296 328 3648 Size: 219 32 45 32 3320 Fragments arranged by size: 3320 219 45 32 32 XbaI T'CTAG_A Cuts at: 0 1987 3648 Size: 1987 1661 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1395 3648 Size: 1395 2253 ZraI GAC'GTC Cuts at: 0 144 3648 Size: 144 3504 Enzymes that do cut: AarI AatII AccI AclI AcuI AleI AlfI AlwNI ApoI AseI AvaI BaeI BanI BbeI BcgI BciVI BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrGI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII HindIII HpaI KasI MfeI MmeI MscI MslI MspA1I NarI NcoI NdeI NsiI PasI PfoI PmlI PpuMI PsrI PstI PvuII SapI ScaI SexAI SfcI SfoI SmlI SspI StyI TaqII TatI TstI XbaI XcmI ZraI Enzymes that do not cut: Acc65I AfeI AflII AflIII AgeI AhdI AloI ApaI ApaLI AscI AsiSI AvrII BamHI BanII BbsI BbvCI BlpI BmtI BplI BsaI BsaBI BseYI BsiHKAI BsiWI BsrBI BsrFI BssHII BssSI BstZ17I BtgZI EcoICRI FalI FseI FspI FspAI KpnI MluI NaeI NgoMIV NheI NotI NruI NspI PacI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XhoI XmaI XmnI