Restriction map for
pFN21AE5667
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BcgI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI Bsp1286I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII HapII HhaI HinP1I
HindIII HpaI HpaII KasI KpnI
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI PacI PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfcI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
SwaI Tth111I Van91I XbaI XcmI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AcyI 9 GGCGTC G[GA]^CG[CT]C
AlwI 668 GGATC GGATC(4/5)
AlwNI 322 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 1264 CTCGAG C^[CT]CG[GA]G
AvaII 277 GGACC G^G[AT]CC
BanI 20 GGCACC G^G[CT][GA]CC
BbvCI 4 GCTGAGG CCTCAGC(-5/-2)
BciVI 595 GGATAC GTATCC(6/5)
BsaHI 9 GGCGTC G[GA]^CG[CT]C
BseRI 811 CTCCTC GAGGAG(10/8)
BsmFI 679 GGGAC GGGAC(10/14)
BsoBI 1264 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1014 TGTACA T^GTACA
BsrGI 1014 TGTACA T^GTACA
BssKI 211 CCTGG ^CC[ACGT]GG
BstBI 36 TTCGAA TT^CGAA
BstF5I 143 GGATG GGATG(2/0)
BstNI 211 CCTGG CC^[AT]GG
Cfr13I 277 GGACC G^G[ACGT]CC
DpnII 669 GATC ^GATC
DraI 336 TTTAAA TTT^AAA
DraII 276 AGGACCC [GA]G^G[ACGT]CC[CT]
EarI 1346 GAAGAG CTCTTC(1/4)
EcoO109I 276 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 757 GAATTC G^AATTC
EcoRII 211 CCTGG ^CC[AT]GG
EcoT14I 556 CCTTGG C^C[AT][AT]GG
FauI 29 GCGGG CCCGC(4/6)
FokI 143 GGATG GGATG(9/13)
HgaI 10 GCGTC GACGC(5/10)
Hin1I 9 GGCGTC G[GA]^CG[CT]C
HincII 395 GTCAAC GT[CT]^[GA]AC
HindII 395 GTCAAC GT[CT]^[GA]AC
Ksp632I 1346 GAAGAG CTCTTC(1/4)
MboI 669 GATC ^GATC
MslI 201 CACTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 211 CCTGG CC^[AT]GG
NdeI 157 CATATG CA^TATG
NdeII 669 GATC ^GATC
NspV 36 TTCGAA TT^CGAA
PaeR7I 1264 CTCGAG C^TCGAG
PpuMI 276 AGGACCC [GA]G^G[AT]CC[CT]
Sau3AI 669 GATC ^GATC
Sau96I 277 GGACC G^G[ACGT]CC
ScaI 1051 AGTACT AGT^ACT
ScrFI 211 CCTGG CC^[ACGT]GG
SfaNI 353 GCATC GCATC(5/9)
SfuI 36 TTCGAA TT^CGAA
SphI 1084 GCATGC GCATG^C
SspBI 1014 TGTACA T^GTACA
SspI 1061 AATATT AAT^ATT
StyI 556 CCTTGG C^C[AT][AT]GG
TspRI 562 CACTG CA[GC]TG[ACGT][ACGT]^
XhoI 1264 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 14 enzymes) #
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AseI 514 ATTAAT AT^TAAT
1165 ATTAAT
AsnI 514 ATTAAT AT^TAAT
1165 ATTAAT
BpmI 770 CTGGAG CTGGAG(16/14)
1504 CTGGAG
BsaJI 24 CCGAGG C^C[ACGT][ACGT]GG
556 CCTTGG
Cac8I 1084 GCATGC GC[ACGT]^[ACGT]GC
1088 GCTGGC
DdeI 5 CTGAG C^T[ACGT]AG
814 CTCAG
HaeIII 505 GGCC GG^CC
1214 GGCC
HinfI 304 GATTC G^A[ACGT]TC
1371 GATTC
HphI 214 GGTGA GGTGA(8/7)
1548 GGTGA
NlaIV 20 GGCACC GG[ACGT]^[ACGT]CC
277 GGACCC
NspI 418 ACATGC [GA]CATG^[CT]
1084 GCATGC
PshBI 514 ATTAAT AT^TAAT
1165 ATTAAT
TfiI 304 GATTC G^A[AT]TC
1371 GATTC
Tsp45I 12 GTCAC ^GT[GC]AC
1459 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I BbsI BbvI BfaI BpuAI
BsiYI BslI BsrI Fnu4HI ItaI
MaeI MaeIII MboII MnlI MseI
MspA1I MwoI NlaIII PvuII RsaI
SmlI TaqI Tru9I TseI Tsp509I
TthHB8I XmnI