(Circular) (Six-base) MAPSORT of: pFN21AE5667.seq Check: 774 from: 1 to: 6274 pFN21AE5667 6274 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4084 278 Size: 53 83 186 936 2548 2468 Fragments arranged by size: 2548 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3617 1937 Size: 1680 4594 Acc65I G'GTAC_C Cuts at: 3595 3595 Size: 6274 AclI AA'CG_TT Cuts at: 1563 4401 4774 1563 Size: 2838 373 3063 Fragments arranged by size: 3063 2838 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2075 3207 3305 4337 5721 5989 1632 Size: 443 1132 98 1032 1384 268 1917 Fragments arranged by size: 1917 1384 1132 1032 443 268 98 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6077 AflIII A'CryG_T Cuts at: 1203 5173 6150 1203 Size: 3970 977 1327 Fragments arranged by size: 3970 1327 977 AgeI A'CCGG_T Cuts at: 5105 5105 Size: 6274 AhdI GACnn_n'nnGTC Cuts at: 5003 5003 Size: 6274 AleI CACnn'nnGTG Cuts at: 1166 2210 1166 Size: 1044 5230 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6274 AlwNI CAG_nnn'CTG Cuts at: 2332 4017 5589 2332 Size: 1685 1572 3017 Fragments arranged by size: 3017 1685 1572 ApaLI G'TGCA_C Cuts at: 4331 5487 4331 Size: 1156 5118 ApoI r'AATT_y Cuts at: 1493 2762 2921 3024 3275 3441 3476 3583 3809 Size: 1269 159 103 251 166 35 107 226 Cuts at: 3809 3961 6070 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1269 251 226 166 159 152 107 103 35 AseI AT'TA_AT Cuts at: 160 1032 2520 3171 4828 160 Size: 872 1488 651 1657 1606 Fragments arranged by size: 1657 1606 1488 872 651 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6274 AvaI C'yCGr_G Cuts at: 1870 1943 3269 3599 1870 Size: 73 1326 330 4545 Fragments arranged by size: 4545 1326 330 73 BamHI G'GATC_C Cuts at: 3604 3971 3604 Size: 367 5907 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2025 3595 5050 618 Size: 325 217 627 238 1570 1455 1842 Fragments arranged by size: 1842 1570 1455 627 325 238 217 BanII G_rGCy'C Cuts at: 1391 3593 1391 Size: 2202 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2499 2881 3312 928 Size: 971 600 382 431 3890 Fragments arranged by size: 3890 971 600 431 382 BbvCI CC'TCA_GC Cuts at: 2010 2010 Size: 6274 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4465 4499 1903 Size: 34 2528 34 3678 Fragments arranged by size: 3678 2528 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2594 4166 5376 2594 Size: 1572 1210 3492 Fragments arranged by size: 3492 1572 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6274 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4885 136 Size: 107 122 71 631 605 3213 1525 Fragments arranged by size: 3213 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6269 6269 Size: 6274 BlpI GC'TnA_GC Cuts at: 3691 3691 Size: 6274 Bme1580I G_kGCm'C Cuts at: 1790 4335 5491 1790 Size: 2545 1156 2573 Fragments arranged by size: 2573 2545 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6274 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2314 2578 4005 Size: 416 53 83 399 24 897 264 1427 Cuts at: 4005 4963 442 Size: 958 1753 Fragments arranged by size: 1753 1427 958 897 416 399 264 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6274 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2796 3530 4934 1239 Size: 1557 734 1404 2579 Fragments arranged by size: 2579 1557 1404 734 Bpu10I CC'TnA_GC Cuts at: 2010 2010 Size: 6274 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3437 3453 4399 5264 5562 3437 Size: 16 946 865 298 4149 Fragments arranged by size: 4149 946 865 298 16 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4937 882 Size: 617 3438 2219 Fragments arranged by size: 3438 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4065 493 Size: 711 2861 2702 Fragments arranged by size: 2861 2702 711 BsaBI GATnn'nnATC Cuts at: 6268 6268 Size: 6274 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2015 4081 4463 5098 Size: 53 83 186 936 482 2066 382 635 Cuts at: 5098 275 Size: 1451 Fragments arranged by size: 2066 1451 936 635 482 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4707 5105 5379 5526 5937 4707 Size: 398 274 147 411 5044 Fragments arranged by size: 5044 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6049 Fragments arranged by size: 6049 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2807 1186 Size: 1621 4653 BseYI C'CCAG_C Cuts at: 5477 5973 5477 Size: 496 5778 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5701 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3724 4485 5513 1540 Size: 275 186 1723 761 1028 2301 Fragments arranged by size: 2301 1723 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4335 4420 5491 4335 Size: 85 1071 5118 Fragments arranged by size: 5118 1071 85 BsmI GAATG_Cn' Cuts at: 3785 3878 3785 Size: 93 6181 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3593 4335 4420 5491 1391 Size: 399 1803 742 85 1071 2174 Fragments arranged by size: 2174 1803 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5937 5937 Size: 6274 BspHI T'CATG_A Cuts at: 4163 5893 4163 Size: 1730 4544 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3629 844 Size: 2785 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4161 1394 Size: 429 2338 3507 Fragments arranged by size: 3507 2338 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4769 4943 65 Size: 1183 3521 174 1396 Fragments arranged by size: 3521 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4918 5105 1700 Size: 110 3108 187 2869 Fragments arranged by size: 3108 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3019 96 Size: 2923 3351 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6274 BssSI C'ACGA_G Cuts at: 4334 5346 4334 Size: 1012 5262 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6274 BstBI TT'CG_AA Cuts at: 2042 5078 2042 Size: 3036 3238 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6274 BstYI r'GATC_y Cuts at: 1972 3604 3971 4356 4373 5825 5911 5923 6269 Size: 1632 367 385 17 1452 86 12 346 Cuts at: 6269 1972 Size: 1977 Fragments arranged by size: 1977 1632 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4783 Fragments arranged by size: 4783 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5535 Fragments arranged by size: 5535 683 56 BtsI GCAGTG_nn' Cuts at: 3790 4584 4604 3790 Size: 794 20 5460 Fragments arranged by size: 5460 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6274 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6239 DraI TTT'AAA Cuts at: 2343 3578 2343 Size: 1235 5039 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5281 809 Size: 507 3965 1802 Fragments arranged by size: 3965 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3721 4610 6239 Size: 54 1425 50 275 18 1891 889 1629 Cuts at: 6239 8 Size: 43 Fragments arranged by size: 1891 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3721 1537 Size: 275 1909 4090 Fragments arranged by size: 4090 1909 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3346 4204 1353 Size: 1993 858 3423 Fragments arranged by size: 3423 1993 858 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4861 5235 5381 387 Size: 1064 138 3272 374 146 1280 Fragments arranged by size: 3272 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2796 3207 3305 3530 4337 4934 Size: 393 443 721 411 98 225 807 597 Cuts at: 4934 5721 5989 1239 Size: 787 268 1524 Fragments arranged by size: 1524 807 787 721 597 443 411 393 268 225 98 EcoO109I rG'GnC_Cy Cuts at: 2282 2282 Size: 6274 EcoRI G'AATT_C Cuts at: 2762 3583 3961 2762 Size: 821 378 5075 Fragments arranged by size: 5075 821 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2303 3431 2303 Size: 1128 5146 FspI TGC'GCA Cuts at: 4780 4780 Size: 6274 HaeII r_GCGC'y Cuts at: 1678 5421 1678 Size: 3743 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4915 4947 4989 5021 1373 Size: 32 135 32 3343 32 42 32 2626 Fragments arranged by size: 3343 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2402 3618 3864 669 Size: 988 281 464 1216 246 3079 Fragments arranged by size: 3079 1216 988 464 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6274 HpaI GTT'AAC Cuts at: 3864 3864 Size: 6274 KpnI G_GTAC'C Cuts at: 3599 3599 Size: 6274 MfeI C'AATT_G Cuts at: 3873 3873 Size: 6274 MluI A'CGCG_T Cuts at: 6150 6150 Size: 6274 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5388 5572 1305 Size: 4083 184 2007 Fragments arranged by size: 4083 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4452 Fragments arranged by size: 4452 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2210 4232 4591 4750 518 Size: 648 1044 2022 359 159 2042 Fragments arranged by size: 2042 2022 1044 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2332 2670 3046 3327 3690 4367 5515 5760 Size: 726 338 376 281 363 677 1148 245 Cuts at: 5760 1606 Size: 2120 Fragments arranged by size: 2120 1148 726 677 376 363 338 281 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4783 Fragments arranged by size: 4783 939 552 NdeI CA'TA_TG Cuts at: 387 2163 387 Size: 1776 4498 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6274 NotI GC'GGCC_GC Cuts at: 3721 3721 Size: 6274 NspI r_CATG'y Cuts at: 1132 1229 2427 3093 3632 5177 1132 Size: 97 1198 666 539 1545 2229 Fragments arranged by size: 2229 1545 1198 666 539 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6274 PciI A'CATG_T Cuts at: 5173 5173 Size: 6274 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6274 PmeI GTTT'AAAC Cuts at: 3578 3578 Size: 6274 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4343 4375 5856 5888 4343 Size: 32 1481 32 4729 Fragments arranged by size: 4729 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2282 2282 Size: 6274 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6274 PsiI TTA'TAA Cuts at: 3844 3844 Size: 6274 PstI C_TGCA'G Cuts at: 830 3626 830 Size: 2796 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6274 PvuII CAG'CTG Cuts at: 2332 2670 3046 2332 Size: 338 376 5560 Fragments arranged by size: 5560 376 338 SalI G'TCGA_C Cuts at: 1936 3616 1936 Size: 1680 4594 SbfI CC_TGCA'GG Cuts at: 3626 3626 Size: 6274 ScaI AGT'ACT Cuts at: 3058 4522 6234 3058 Size: 1464 1712 3098 Fragments arranged by size: 3098 1712 1464 SfcI C'TryA_G Cuts at: 826 1045 3622 4757 5438 5629 826 Size: 219 2577 1135 681 191 1471 Fragments arranged by size: 2577 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6274 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6274 SmaI CCC'GGG Cuts at: 1872 3601 1872 Size: 1729 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3269 3416 3468 4378 5279 5541 Size: 197 926 1326 147 52 910 901 262 Cuts at: 5541 820 Size: 1553 Fragments arranged by size: 1553 1326 926 910 901 262 197 147 52 SnaBI TAC'GTA Cuts at: 493 4065 493 Size: 3572 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6274 SphI G_CATG'C Cuts at: 1132 3093 3632 1132 Size: 1961 539 3774 Fragments arranged by size: 3774 1961 539 SspI AAT'ATT Cuts at: 5 52 3068 4198 5 Size: 47 3016 1130 2081 Fragments arranged by size: 3016 2081 1130 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6274 StyI C'CwwG_G Cuts at: 513 1065 2004 2561 3713 513 Size: 552 939 557 1152 3074 Fragments arranged by size: 3074 1152 939 557 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4301 4318 4471 4656 5101 325 Size: 1255 2721 17 153 185 445 1498 Fragments arranged by size: 2721 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2301 3019 3056 Size: 274 80 33 51 1443 324 718 37 Cuts at: 3056 4520 6232 96 Size: 1464 1712 138 Fragments arranged by size: 1712 1464 1443 718 324 274 138 80 51 37 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6234 6266 6234 Size: 32 6242 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6274 XbaI T'CTAG_A Cuts at: 3610 3610 Size: 6274 XhoI C'TCGA_G Cuts at: 1943 3269 1943 Size: 1326 4948 XmaI C'CCGG_G Cuts at: 1870 3599 1870 Size: 1729 4545 XmnI GAAnn'nnTTC Cuts at: 3155 3477 3562 4403 3155 Size: 322 85 841 5026 Fragments arranged by size: 5026 841 322 85 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4082 276 Size: 53 83 186 936 2548 2468 Fragments arranged by size: 2548 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI