Restriction map for
pFN21AE5675
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BglII BlnI BsaAI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI BstEII BstPI BstUI
Bsu36I Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FauI FbaI FseI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MluI
MroI MvnI NaeI NarI NcoI
NgoMIV NlaIV NotI NruI PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspI StuI
SwaI TfiI Tth111I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AciI 1225 GCGG CCGC(-3/-1)
AflII 424 CTTAAG C^TTAAG
Alw44I 905 GTGCAC G^TGCAC
AlwNI 935 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 905 GTGCAC G^TGCAC
AviII 770 TGCGCA TGC^GCA
BbsI 1345 GAAGAC GAAGAC(2/6)
BfrI 424 CTTAAG C^TTAAG
BglI 721 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1240 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1240 GCTCAGC GC^T[ACGT]AGC
BpuAI 1345 GAAGAC GAAGAC(2/6)
BseRI 371 CTCCTC GAGGAG(10/8)
BsmFI 1338 GGGAC GGGAC(10/14)
Bsp1407I 1170 TGTACA T^GTACA
BsrDI 1249 GCAATG GCAATG(2/0)
BsrGI 1170 TGTACA T^GTACA
Bst1107I 1076 GTATAC GTA^TAC
BstZ17I 1076 GTATAC GTA^TAC
CelII 1240 GCTCAGC GC^T[ACGT]AGC
EarI 1032 CTCTTC CTCTTC(1/4)
EcoT22I 755 ATGCAT ATGCA^T
FspI 770 TGCGCA TGC^GCA
HaeII 1560 GGCGCT [GA]GCGC^[CT]
HgaI 4 GACGC GACGC(5/10)
HinfI 1157 GAGTC G^A[ACGT]TC
Ksp632I 1032 CTCTTC CTCTTC(1/4)
MfeI 1570 CAATTG C^AATTG
MspA1I 995 CAGCTG C[AC]G^C[GT]G
MunI 1570 CAATTG C^AATTG
NdeI 929 CATATG CA^TATG
NheI 1245 GCTAGC G^CTAGC
NsiI 755 ATGCAT ATGCA^T
NspI 757 GCATGC [GA]CATG^[CT]
PflMI 955 CCAGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1157 GAGTC GAGTC(4/5)
PpuMI 516 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1348 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 469 CTGCAG CTGCA^G
PvuII 995 CAGCTG CAG^CTG
SacI 1007 GAGCTC GAGCT^C
SphI 757 GCATGC GCATG^C
SspBI 1170 TGTACA T^GTACA
Van91I 955 CCAGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 722 CCAAAATGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 254 GTCTAC GT^[AC][GT]AC
1076 GTATAC
AfaI 1171 GTAC GT^AC
1321 GTAC
AlwI 64 GGATC GGATC(4/5)
1576 GGATC
AspHI 905 GTGCAC G[AT]GC[AT]^C
1007 GAGCTC
AvaI 452 CTCGAG C^[CT]CG[GA]G
1331 CTCGAG
BcnI 910 CCGGG CC^[GC]GG
1402 CCGGG
BfaI 1070 CTAG C^TAG
1246 CTAG
BpmI 512 CTGGAG CTGGAG(16/14)
1004 CTGGAG
BsaBI 1168 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1228 GATGCAAATC
BsgI 464 CTGCAC GTGCAG(16/14)
762 CTGCAC
BsiHKAI 905 GTGCAC G[AT]GC[AT]^C
1007 GAGCTC
BsmAI 830 GAGAC GTCTC(1/5)
1272 GAGAC
BsoBI 452 CTCGAG C^[CT]CG[GA]G
1331 CTCGAG
BstAPI 712 GCACATTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
934 GCAGGGGCTGC
BstXI 1257 CCATCTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1620 CCATGACCTTGG
BstYI 64 GGATCT [GA]^GATC[CT]
1576 GGATCT
CfoI 771 GCGC GCG^C
1561 GCGC
DraII 516 AGGTCCT [GA]G^G[ACGT]CC[CT]
1598 AGGCCCT
EcoO109I 516 AGGTCCT [GA]G^G[ACGT]CC[CT]
1598 AGGCCCT
HapII 910 CCGG C^CGG
1402 CCGG
HhaI 771 GCGC GCG^C
1561 GCGC
HinP1I 771 GCGC G^CGC
1561 GCGC
HpaII 910 CCGG C^CGG
1402 CCGG
MaeI 1070 CTAG C^TAG
1246 CTAG
MamI 1168 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1228 GATGCAAATC
MflI 64 GGATCT [GA]^GATC[CT]
1576 GGATCT
MslI 1258 CATCTAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1615 CATAACCATG
MspI 910 CCGG C^CGG
1402 CCGG
NciI 910 CCGGG CC^[GC]GG
1402 CCGGG
PaeR7I 452 CTCGAG C^TCGAG
1331 CTCGAG
RsaI 1171 GTAC GT^AC
1321 GTAC
XhoI 452 CTCGAG C^TCGAG
1331 CTCGAG
XhoII 64 GGATCT [GA]^GATC[CT]
1576 GGATCT
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AcsI AluI ApoI Asp700I AvaII
BalI BanII BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstBI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI EaeI EcoRII
EcoT14I Fnu4HI FokI HaeIII HphI
ItaI MaeIII MboI MboII MluNI
MnlI MscI MseI MvaI MwoI
NdeII NlaIII NspV Sau3AI Sau96I
ScrFI SfaNI SfcI SfuI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmnI