Restriction map for
pFN21AE5675
(Circular) (Six-base) MAPSORT of: pFN21AE5675.seq Check: 4404 from: 1 to: 6379
pFN21AE5675 6379 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4189 278
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2260 3082 3722 1937
Size: 323 822 640 4594
Fragments arranged by size:
4594 822 640 323
Acc65I G'GTAC_C
Cuts at: 3700 3700
Size: 6379
AclI AA'CG_TT
Cuts at: 1563 4506 4879 1563
Size: 2943 373 3063
Fragments arranged by size:
3063 2943 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2678 3515 4442 5826 6094 1632
Size: 1046 837 927 1384 268 1917
Fragments arranged by size:
1917 1384 1046 927 837 268
AflII C'TTAA_G
Cuts at: 820 1017 2429 820
Size: 197 1412 4770
Fragments arranged by size:
4770 1412 197
AflIII A'CryG_T
Cuts at: 1203 5278 6255 1203
Size: 4075 977 1327
Fragments arranged by size:
4075 1327 977
AgeI A'CCGG_T
Cuts at: 5210 5210
Size: 6379
AhdI GACnn_n'nnGTC
Cuts at: 5108 5108
Size: 6379
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6379
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6379
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3081 3113 3081
Size: 32 6347
AlwNI CAG_nnn'CTG
Cuts at: 2945 4122 5694 2945
Size: 1177 1572 3630
Fragments arranged by size:
3630 1572 1177
ApaLI G'TGCA_C
Cuts at: 2910 4436 5592 2910
Size: 1526 1156 3697
Fragments arranged by size:
3697 1526 1156
ApoI r'AATT_y
Cuts at: 1493 2162 2217 2633 3091 3539 3688 3914 4066
Size: 669 55 416 458 448 149 226 152
Cuts at: 4066 6175 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 669 458 448 416 226 152
149 55
AseI AT'TA_AT
Cuts at: 160 1032 4933 160
Size: 872 3901 1606
Fragments arranged by size:
3901 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
AvaI C'yCGr_G
Cuts at: 1870 1943 2457 3336 3704 1870
Size: 73 514 879 368 4545
Fragments arranged by size:
4545 879 514 368 73
BamHI G'GATC_C
Cuts at: 3709 4076 3709
Size: 367 6012
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3700 5155 618
Size: 325 217 627 1913 1455 1842
Fragments arranged by size:
1913 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2464 2561 3016 3413 3698 1391
Size: 1073 97 455 397 285 4072
Fragments arranged by size:
4072 1073 455 397 285 97
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3357 928
Size: 971 1458 3950
Fragments arranged by size:
3950 1458 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4570 4604 1903
Size: 34 2633 34 3678
Fragments arranged by size:
3678 2633 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4271 5481 4271
Size: 1210 5169
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6379
BfrBI ATG'CAT
Cuts at: 2762 2762
Size: 6379
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2732 4990 136
Size: 107 122 71 631 605 1060 2258 1525
Fragments arranged by size:
2258 1525 1060 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6374 6374
Size: 6379
BlpI GC'TnA_GC
Cuts at: 3246 3796 3246
Size: 550 5829
Bme1580I G_kGCm'C
Cuts at: 1790 2914 4440 5596 1790
Size: 1124 1526 1156 2573
Fragments arranged by size:
2573 1526 1156 1124
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6379
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2702 4110 5068
Size: 416 53 83 399 24 1285 1408 958
Cuts at: 5068 442
Size: 1753
Fragments arranged by size:
1753 1408 1285 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3254 1056
Size: 2198 4181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2538 3030 5039 1239
Size: 1299 492 2009 2579
Fragments arranged by size:
2579 2009 1299 492
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2961 4504 5369 5667 2961
Size: 1543 865 298 3673
Fragments arranged by size:
3673 1543 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5042 882
Size: 617 3543 2219
Fragments arranged by size:
3543 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4170 493
Size: 711 2966 2702
Fragments arranged by size:
2966 2702 711
BsaBI GATnn'nnATC
Cuts at: 3177 3237 6373 3177
Size: 60 3136 3183
Fragments arranged by size:
3183 3136 60
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4186 4568 5203 275
Size: 53 83 186 936 2653 382 635 1451
Fragments arranged by size:
2653 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4812 5210 5484 5631 6042 4812
Size: 398 274 147 411 5149
Fragments arranged by size:
5149 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6154
Fragments arranged by size:
6154 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2367 1186
Size: 1181 5198
BseYI C'CCAG_C
Cuts at: 2959 5582 6078 2959
Size: 2623 496 3260
Fragments arranged by size:
3260 2623 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2454 2752 1163
Size: 573 718 298 4790
Fragments arranged by size:
4790 718 573 298
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3829 4590 5618 1540
Size: 275 186 1828 761 1028 2301
Fragments arranged by size:
2301 1828 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2914 3016 4440 4525 5596 2914
Size: 102 1424 85 1071 3697
Fragments arranged by size:
3697 1424 1071 102 85
BsmI GAATG_Cn'
Cuts at: 3890 3983 3890
Size: 93 6286
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2464 2561 2914 3016 3413 3698 4440
Size: 399 674 97 353 102 397 285 742
Cuts at: 4440 4525 5596 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 674 399 397 353 285
102 97 85
BspEI T'CCGG_A
Cuts at: 6042 6042
Size: 6379
BspHI T'CATG_A
Cuts at: 4268 5998 4268
Size: 1730 4649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3734 844
Size: 2890 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4266 1394
Size: 429 2443 3507
Fragments arranged by size:
3507 2443 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3261 4874 5048 65
Size: 1183 2013 1613 174 1396
Fragments arranged by size:
2013 1613 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5023 5210 1700
Size: 110 3213 187 2869
Fragments arranged by size:
3213 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3175 96
Size: 3079 3300
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6379
BssSI C'ACGA_G
Cuts at: 4439 5451 4439
Size: 1012 5367
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2723 2945 794
Size: 1929 222 4228
Fragments arranged by size:
4228 1929 222
BstBI TT'CG_AA
Cuts at: 2212 2386 2569 5183 2212
Size: 174 183 2614 3408
Fragments arranged by size:
3408 2614 183 174
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6379
BstXI CCAn_nnnn'nTGG
Cuts at: 3269 3632 3269
Size: 363 6016
BstYI r'GATC_y
Cuts at: 1972 2069 3581 3709 4076 4461 4478 5930 6016
Size: 97 1512 128 367 385 17 1452 86
Cuts at: 6016 6028 6374 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1512 1452 385 367 346 128 97
86 17 12
BstZ17I GTA'TAC
Cuts at: 3083 3083
Size: 6379
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4888
Fragments arranged by size:
4888 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3377 487
Size: 683 56 2151 3489
Fragments arranged by size:
3489 2151 683 56
BtsI GCAGTG_nn'
Cuts at: 2471 3895 4689 4709 2471
Size: 1424 794 20 4141
Fragments arranged by size:
4141 1424 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6379
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6344
DraI TTT'AAA
Cuts at: 2167 3400 3538 3683 2167
Size: 1233 138 145 4863
Fragments arranged by size:
4863 1233 145 138
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5386 809
Size: 507 4070 1802
Fragments arranged by size:
4070 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2743 2869 3060
Size: 54 1425 50 275 18 913 126 191
Cuts at: 3060 3826 4715 6344 8
Size: 766 889 1629 43
Fragments arranged by size:
1629 1425 913 889 766 275 191 126
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3826 1537
Size: 275 2014 4090
Fragments arranged by size:
4090 2014 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3043 4309 1353
Size: 1690 1266 3423
Fragments arranged by size:
3423 1690 1266
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3245 4966 5340 5486 387
Size: 1064 138 1656 1721 374 146 1280
Fragments arranged by size:
1721 1656 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3014 3014
Size: 6379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2538 2678 3030 3515 4442 5039 5826
Size: 393 906 140 352 485 927 597 787
Cuts at: 5826 6094 1239
Size: 268 1524
Fragments arranged by size:
1524 927 906 787 597 485 393 352
268 140
EcoO109I rG'GnC_Cy
Cuts at: 2522 3604 2522
Size: 1082 5297
EcoRI G'AATT_C
Cuts at: 3688 4066 3688
Size: 378 6001
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2328 2708 2328
Size: 380 5999
FspI TGC'GCA
Cuts at: 2777 4885 2777
Size: 2108 4271
HaeII r_GCGC'y
Cuts at: 1678 3569 5526 1678
Size: 1891 1957 2531
Fragments arranged by size:
2531 1957 1891
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2234 2266 2425 2457 5020
Size: 32 135 32 662 32 159 32 2563
Cuts at: 5020 5052 5094 5126 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2563 662 159 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3723 3969 669
Size: 988 281 1785 246 3079
Fragments arranged by size:
3079 1785 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6379
HpaI GTT'AAC
Cuts at: 3969 3969
Size: 6379
KpnI G_GTAC'C
Cuts at: 3704 3704
Size: 6379
MfeI C'AATT_G
Cuts at: 3575 3978 3575
Size: 403 5976
MluI A'CGCG_T
Cuts at: 6255 6255
Size: 6379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3486 5493 5677 1305
Size: 2181 2007 184 2007
Fragments arranged by size:
2181 2007 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2745 2871 3062 10
Size: 54 1425 343 913 126 191 3327
Fragments arranged by size:
3327 1425 913 343 191 126 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3267 3624 4337 4696 4855 518
Size: 648 2101 357 713 359 159 2042
Fragments arranged by size:
2101 2042 713 648 359 357 159
MspA1I CmG'CkG
Cuts at: 1606 3002 3795 4472 5620 5865 1606
Size: 1396 793 677 1148 245 2120
Fragments arranged by size:
2120 1396 1148 793 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4888
Fragments arranged by size:
4888 939 552
NdeI CA'TA_TG
Cuts at: 387 2935 387
Size: 2548 3831
NheI G'CTAG_C
Cuts at: 1052 3250 1052
Size: 2198 4181
NotI GC'GGCC_GC
Cuts at: 3826 3826
Size: 6379
NsiI A_TGCA'T
Cuts at: 2764 2764
Size: 6379
NspI r_CATG'y
Cuts at: 1132 1229 2766 3737 5282 1132
Size: 97 1537 971 1545 2229
Fragments arranged by size:
2229 1545 1537 971 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6379
PciI A'CATG_T
Cuts at: 5278 5278
Size: 6379
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2966 1108
Size: 1858 4521
PfoI T'CCnGG_A
Cuts at: 3007 3007
Size: 6379
PmeI GTTT'AAAC
Cuts at: 3683 3683
Size: 6379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4448 4480 5961 5993 4448
Size: 32 1481 32 4834
Fragments arranged by size:
4834 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2522 2522
Size: 6379
PshAI GACnn'nnGTC
Cuts at: 1613 3357 1613
Size: 1744 4635
PsiI TTA'TAA
Cuts at: 3949 3949
Size: 6379
PspXI vC'TCGA_Gb
Cuts at: 2457 2457
Size: 6379
PstI C_TGCA'G
Cuts at: 830 2478 3731 830
Size: 1648 1253 3478
Fragments arranged by size:
3478 1648 1253
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6379
PvuII CAG'CTG
Cuts at: 3002 3002
Size: 6379
SacI G_AGCT'C
Cuts at: 3016 3016
Size: 6379
SalI G'TCGA_C
Cuts at: 1936 3721 1936
Size: 1785 4594
SbfI CC_TGCA'GG
Cuts at: 3731 3731
Size: 6379
ScaI AGT'ACT
Cuts at: 4627 6339 4627
Size: 1712 4667
SfcI C'TryA_G
Cuts at: 826 1045 2136 2474 2982 3727 4862 5543 5734
Size: 219 1091 338 508 745 1135 681 191
Cuts at: 5734 826
Size: 1471
Fragments arranged by size:
1471 1135 1091 745 681 508 338 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6379
SmaI CCC'GGG
Cuts at: 1872 3706 1872
Size: 1834 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2429 2457 2976 3336 4483 5384
Size: 197 926 486 28 519 360 1147 901
Cuts at: 5384 5646 820
Size: 262 1553
Fragments arranged by size:
1553 1147 926 901 519 486 360 262
197 28
SnaBI TAC'GTA
Cuts at: 493 4170 493
Size: 3677 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6379
SphI G_CATG'C
Cuts at: 1132 2766 3737 1132
Size: 1634 971 3774
Fragments arranged by size:
3774 1634 971
SspI AAT'ATT
Cuts at: 5 52 4303 5
Size: 47 4251 2081
Fragments arranged by size:
4251 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6379
StyI C'CwwG_G
Cuts at: 513 1065 2004 3216 3355 3631 3818 513
Size: 552 939 1212 139 276 187 3074
Fragments arranged by size:
3074 1212 939 552 276 187 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4406 4423 4576 4761 5206 325
Size: 1255 2826 17 153 185 445 1498
Fragments arranged by size:
2826 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3175 3325 4625
Size: 274 80 33 51 1443 1198 150 1300
Cuts at: 4625 6337 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1300 1198 274 150 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3510 3542 6339 6371 3510
Size: 32 2797 32 3518
Fragments arranged by size:
3518 2797 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6379
XbaI T'CTAG_A
Cuts at: 3715 3715
Size: 6379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2734 2734
Size: 6379
XhoI C'TCGA_G
Cuts at: 1943 2457 3336 1943
Size: 514 879 4986
Fragments arranged by size:
4986 879 514
XmaI C'CCGG_G
Cuts at: 1870 3704 1870
Size: 1834 4545
XmnI GAAnn'nnTTC
Cuts at: 2613 3503 3597 4508 2613
Size: 890 94 911 4484
Fragments arranged by size:
4484 911 890 94
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4187 276
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI