(Linear) (Six-base) MAPSORT of: KIEE5675.seq Check: 6535 from: 1 to: 1674 KIEE5675.orf With 182 enzymes: SgfI * December 5, 2014 13:06 .. AccI GT'mk_AC Cuts at: 0 255 1077 1674 Size: 255 822 597 Fragments arranged by size: 822 597 255 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 673 1510 1674 Size: 673 837 164 Fragments arranged by size: 837 673 164 AflII C'TTAA_G Cuts at: 0 424 1674 Size: 424 1250 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1076 1108 1674 Size: 1076 32 566 Fragments arranged by size: 1076 566 32 AlwNI CAG_nnn'CTG Cuts at: 0 940 1674 Size: 940 734 ApaLI G'TGCA_C Cuts at: 0 905 1674 Size: 905 769 ApoI r'AATT_y Cuts at: 0 157 212 628 1086 1534 1674 Size: 157 55 416 458 448 140 Fragments arranged by size: 458 448 416 157 140 55 AvaI C'yCGr_G Cuts at: 0 452 1331 1674 Size: 452 879 343 Fragments arranged by size: 879 452 343 BanII G_rGCy'C Cuts at: 0 459 556 1011 1408 1674 Size: 459 97 455 397 266 Fragments arranged by size: 459 455 397 266 97 BbsI GAAGACnn'nnnn_ Cuts at: 0 1352 1674 Size: 1352 322 BfrBI ATG'CAT Cuts at: 0 757 1674 Size: 757 917 BglI GCCn_nnn'nGGC Cuts at: 0 727 1674 Size: 727 947 BlpI GC'TnA_GC Cuts at: 0 1241 1674 Size: 1241 433 Bme1580I G_kGCm'C Cuts at: 0 909 1674 Size: 909 765 BmrI ACTGGGnnnn_n' Cuts at: 0 697 1674 Size: 697 977 BmtI G_CTAG'C Cuts at: 0 1249 1674 Size: 1249 425 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 533 1025 1674 Size: 533 492 649 Fragments arranged by size: 649 533 492 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 956 1674 Size: 956 718 BsaBI GATnn'nnATC Cuts at: 0 1172 1232 1674 Size: 1172 60 442 Fragments arranged by size: 1172 442 60 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 362 1674 Size: 362 1312 BseYI C'CCAG_C Cuts at: 0 954 1674 Size: 954 720 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 449 747 1674 Size: 449 298 927 Fragments arranged by size: 927 449 298 BsiHKAI G_wGCw'C Cuts at: 0 909 1011 1674 Size: 909 102 663 Fragments arranged by size: 909 663 102 Bsp1286I G_dGCh'C Cuts at: 0 459 556 909 1011 1408 1674 Size: 459 97 353 102 397 266 Fragments arranged by size: 459 397 353 266 102 97 BsrDI GCAATG_nn' Cuts at: 0 1256 1674 Size: 1256 418 BsrGI T'GTAC_A Cuts at: 0 1170 1674 Size: 1170 504 BstAPI GCAn_nnn'nTGC Cuts at: 0 718 940 1674 Size: 718 222 734 Fragments arranged by size: 734 718 222 BstBI TT'CG_AA Cuts at: 0 207 381 564 1674 Size: 207 174 183 1110 Fragments arranged by size: 1110 207 183 174 BstXI CCAn_nnnn'nTGG Cuts at: 0 1264 1627 1674 Size: 1264 363 47 Fragments arranged by size: 1264 363 47 BstYI r'GATC_y Cuts at: 0 64 1576 1674 Size: 64 1512 98 Fragments arranged by size: 1512 98 64 BstZ17I GTA'TAC Cuts at: 0 1078 1674 Size: 1078 596 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1372 1674 Size: 1372 302 BtsI GCAGTG_nn' Cuts at: 0 466 1674 Size: 466 1208 DraI TTT'AAA Cuts at: 0 162 1395 1533 1674 Size: 162 1233 138 141 Fragments arranged by size: 1233 162 141 138 EaeI y'GGCC_r Cuts at: 0 738 864 1055 1674 Size: 738 126 191 619 Fragments arranged by size: 738 619 191 126 EarI CTCTTCn'nnn_ Cuts at: 0 1038 1674 Size: 1038 636 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1240 1674 Size: 1240 434 EcoICRI GAG'CTC Cuts at: 0 1009 1674 Size: 1009 665 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 533 673 1025 1510 1674 Size: 533 140 352 485 164 Fragments arranged by size: 533 485 352 164 140 EcoO109I rG'GnC_Cy Cuts at: 0 517 1599 1674 Size: 517 1082 75 Fragments arranged by size: 1082 517 75 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 323 703 1674 Size: 323 380 971 Fragments arranged by size: 971 380 323 FspI TGC'GCA Cuts at: 0 772 1674 Size: 772 902 HaeII r_GCGC'y Cuts at: 0 1564 1674 Size: 1564 110 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 229 261 420 452 1674 Size: 229 32 159 32 1222 Fragments arranged by size: 1222 229 159 32 32 MfeI C'AATT_G Cuts at: 0 1570 1674 Size: 1570 104 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1481 1674 Size: 1481 193 MscI TGG'CCA Cuts at: 0 740 866 1057 1674 Size: 740 126 191 617 Fragments arranged by size: 740 617 191 126 MslI CAynn'nnrTG Cuts at: 0 1262 1619 1674 Size: 1262 357 55 Fragments arranged by size: 1262 357 55 MspA1I CmG'CkG Cuts at: 0 997 1674 Size: 997 677 NdeI CA'TA_TG Cuts at: 0 930 1674 Size: 930 744 NheI G'CTAG_C Cuts at: 0 1245 1674 Size: 1245 429 NsiI A_TGCA'T Cuts at: 0 759 1674 Size: 759 915 NspI r_CATG'y Cuts at: 0 761 1674 Size: 761 913 PflMI CCAn_nnn'nTGG Cuts at: 0 961 1674 Size: 961 713 PfoI T'CCnGG_A Cuts at: 0 1002 1674 Size: 1002 672 PpuMI rG'GwC_Cy Cuts at: 0 517 1674 Size: 517 1157 PshAI GACnn'nnGTC Cuts at: 0 1352 1674 Size: 1352 322 PspXI vC'TCGA_Gb Cuts at: 0 452 1674 Size: 452 1222 PstI C_TGCA'G Cuts at: 0 473 1674 Size: 473 1201 PvuII CAG'CTG Cuts at: 0 997 1674 Size: 997 677 SacI G_AGCT'C Cuts at: 0 1011 1674 Size: 1011 663 SfcI C'TryA_G Cuts at: 0 131 469 977 1674 Size: 131 338 508 697 Fragments arranged by size: 697 508 338 131 SmlI C'TyrA_G Cuts at: 0 424 452 971 1331 1674 Size: 424 28 519 360 343 Fragments arranged by size: 519 424 360 343 28 SphI G_CATG'C Cuts at: 0 761 1674 Size: 761 913 StyI C'CwwG_G Cuts at: 0 1211 1350 1626 1674 Size: 1211 139 276 48 Fragments arranged by size: 1211 276 139 48 TatI w'GTAC_w Cuts at: 0 1170 1320 1674 Size: 1170 150 354 Fragments arranged by size: 1170 354 150 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1505 1537 1674 Size: 1505 32 137 Fragments arranged by size: 1505 137 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 729 1674 Size: 729 945 XhoI C'TCGA_G Cuts at: 0 452 1331 1674 Size: 452 879 343 Fragments arranged by size: 879 452 343 XmnI GAAnn'nnTTC Cuts at: 0 608 1498 1592 1674 Size: 608 890 94 82 Fragments arranged by size: 890 608 94 82 Enzymes that do cut: AccI AcuI AflII AloI AlwNI ApaLI ApoI AvaI BanII BbsI BfrBI BglI BlpI Bme1580I BmrI BmtI BpmI BpuEI BsaBI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrDI BsrGI BstAPI BstBI BstXI BstYI BstZ17I BtgZI BtsI DraI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI FspI HaeII Hin4I MfeI MmeI MscI MslI MspA1I NdeI NheI NsiI NspI PflMI PfoI PpuMI PshAI PspXI PstI PvuII SacI SfcI SmlI SphI StyI TatI TstI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI AlfI ApaI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BglII BmgBI BplI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstEII Bsu36I BtgI ClaI CspCI DraIII DrdI EagI EcoNI EcoRI EcoRV FseI FspAI HincII HindIII HpaI KasI KpnI MluI NaeI NarI NcoI NgoMIV NotI NruI PacI PasI PciI PmeI PmlI PpiI PsiI PspOMI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TaqII Tth111I XbaI XmaI ZraI