(Circular) (Six-base) MAPSORT of: pFN21AE5576.seq Check: 4862 from: 1 to: 5875 pFN21AE5576 5875 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3685 278 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2778 2922 2994 3066 3218 1937 Size: 841 144 72 72 152 4594 Fragments arranged by size: 4594 841 152 144 72 72 Acc65I G'GTAC_C Cuts at: 3196 3196 Size: 5875 AclI AA'CG_TT Cuts at: 1563 4002 4375 1563 Size: 2439 373 3063 Fragments arranged by size: 3063 2439 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2348 3938 5322 5590 1632 Size: 716 1590 1384 268 1917 Fragments arranged by size: 1917 1590 1384 716 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5678 AflIII A'CryG_T Cuts at: 1203 4774 5751 1203 Size: 3571 977 1327 Fragments arranged by size: 3571 1327 977 AgeI A'CCGG_T Cuts at: 4706 4706 Size: 5875 AhdI GACnn_n'nnGTC Cuts at: 4604 4604 Size: 5875 AleI CACnn'nnGTG Cuts at: 1166 3088 1166 Size: 1922 3953 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2132 1184 Size: 948 4927 AlwNI CAG_nnn'CTG Cuts at: 3618 5190 3618 Size: 1572 4303 ApaI G_GGCC'C Cuts at: 2339 2339 Size: 5875 ApaLI G'TGCA_C Cuts at: 3932 5088 3932 Size: 1156 4719 ApoI r'AATT_y Cuts at: 1493 2737 2881 2953 3025 3184 3410 3562 5671 Size: 1244 144 72 72 159 226 152 2109 Cuts at: 5671 1493 Size: 1697 Fragments arranged by size: 2109 1697 1244 226 159 152 144 72 72 AseI AT'TA_AT Cuts at: 160 1032 4429 160 Size: 872 3397 1606 Fragments arranged by size: 3397 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 AvaI C'yCGr_G Cuts at: 1870 1943 3200 1870 Size: 73 1257 4545 Fragments arranged by size: 4545 1257 73 BamHI G'GATC_C Cuts at: 3205 3572 3205 Size: 367 5508 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3196 4651 618 Size: 325 217 627 1409 1455 1842 Fragments arranged by size: 1842 1455 1409 627 325 217 BanII G_rGCy'C Cuts at: 1391 2339 3194 1391 Size: 948 855 4072 Fragments arranged by size: 4072 948 855 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2058 928 Size: 971 159 4745 Fragments arranged by size: 4745 971 159 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4066 4100 1903 Size: 34 2129 34 3678 Fragments arranged by size: 3678 2129 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3767 4977 3767 Size: 1210 4665 BclI T'GATC_A Cuts at: 1548 1548 Size: 5875 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4486 136 Size: 107 122 71 631 605 2814 1525 Fragments arranged by size: 2814 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5870 5870 Size: 5875 BlpI GC'TnA_GC Cuts at: 3292 3292 Size: 5875 Bme1580I G_kGCm'C Cuts at: 1790 2339 3095 3936 5092 1790 Size: 549 756 841 1156 2573 Fragments arranged by size: 2573 1156 841 756 549 BmgBI CAC'GTC Cuts at: 1318 2275 2384 1318 Size: 957 109 4809 Fragments arranged by size: 4809 957 109 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2056 3606 4564 Size: 416 53 83 399 24 639 1550 958 Cuts at: 4564 442 Size: 1753 Fragments arranged by size: 1753 1550 958 639 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2085 1056 Size: 1029 4846 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2029 4535 1239 Size: 790 2506 2579 Fragments arranged by size: 2579 2506 790 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4000 4865 5163 4000 Size: 865 298 4712 Fragments arranged by size: 4712 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4538 882 Size: 617 3039 2219 Fragments arranged by size: 3039 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3666 493 Size: 711 2462 2702 Fragments arranged by size: 2702 2462 711 BsaBI GATnn'nnATC Cuts at: 5869 5869 Size: 5875 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3682 4064 4699 275 Size: 53 83 186 936 2149 382 635 1451 Fragments arranged by size: 2149 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4308 4706 4980 5127 5538 4308 Size: 398 274 147 411 4645 Fragments arranged by size: 4645 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5650 Fragments arranged by size: 5650 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2394 2397 1186 Size: 1208 3 4664 Fragments arranged by size: 4664 1208 3 BseYI C'CCAG_C Cuts at: 2091 2332 5078 5574 2091 Size: 241 2746 496 2392 Fragments arranged by size: 2746 2392 496 241 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2396 1163 Size: 573 660 4642 Fragments arranged by size: 4642 660 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3325 4086 5114 1540 Size: 275 186 1324 761 1028 2301 Fragments arranged by size: 2301 1324 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2368 3936 4021 5092 2368 Size: 1568 85 1071 3151 Fragments arranged by size: 3151 1568 1071 85 BsmI GAATG_Cn' Cuts at: 3386 3479 3386 Size: 93 5782 BsmBI CGTCTCn'nnnn_ Cuts at: 3165 3165 Size: 5875 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2339 2368 3095 3194 3936 4021 5092 Size: 399 549 29 727 99 742 85 1071 Cuts at: 5092 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 727 549 399 99 85 29 BspEI T'CCGG_A Cuts at: 5538 5538 Size: 5875 BspHI T'CATG_A Cuts at: 3764 5494 3764 Size: 1730 4145 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2466 3230 844 Size: 1622 764 3489 Fragments arranged by size: 3489 1622 764 BsrBI CCG'CTC Cuts at: 1394 1823 3762 1394 Size: 429 1939 3507 Fragments arranged by size: 3507 1939 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4370 4544 65 Size: 1183 3122 174 1396 Fragments arranged by size: 3122 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4519 4706 1700 Size: 110 2709 187 2869 Fragments arranged by size: 2869 2709 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5875 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5875 BssSI C'ACGA_G Cuts at: 3935 4947 3935 Size: 1012 4863 BstAPI GCAn_nnn'nTGC Cuts at: 794 2726 2870 2942 3014 794 Size: 1932 144 72 72 3655 Fragments arranged by size: 3655 1932 144 72 72 BstBI TT'CG_AA Cuts at: 4679 4679 Size: 5875 BstEII G'GTnAC_C Cuts at: 1184 2608 2968 3040 1184 Size: 1424 360 72 4019 Fragments arranged by size: 4019 1424 360 72 BstYI r'GATC_y Cuts at: 1972 3205 3572 3957 3974 5426 5512 5524 5870 Size: 1233 367 385 17 1452 86 12 346 Cuts at: 5870 1972 Size: 1977 Fragments arranged by size: 1977 1452 1233 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2779 2923 2995 3067 2779 Size: 144 72 72 5587 Fragments arranged by size: 5587 144 72 72 BtgI C'CryG_G Cuts at: 513 1065 2314 513 Size: 552 1249 4074 Fragments arranged by size: 4074 1249 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5136 Fragments arranged by size: 5136 683 56 BtsI GCAGTG_nn' Cuts at: 2682 2826 3095 3391 4185 4205 2682 Size: 144 269 296 794 20 4352 Fragments arranged by size: 4352 794 296 269 144 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5875 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2370 2405 529 Size: 35 1806 35 3999 Fragments arranged by size: 3999 1806 35 35 DraI TTT'AAA Cuts at: 3179 3179 Size: 5875 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2279 4882 809 Size: 507 963 2603 1802 Fragments arranged by size: 2603 1802 963 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3322 4211 5840 Size: 54 1425 50 275 18 1492 889 1629 Cuts at: 5840 8 Size: 43 Fragments arranged by size: 1629 1492 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3322 1537 Size: 275 1510 4090 Fragments arranged by size: 4090 1510 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3163 3805 1353 Size: 1810 642 3423 Fragments arranged by size: 3423 1810 642 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2449 4462 4836 4982 387 Size: 1064 138 860 2013 374 146 1280 Fragments arranged by size: 2013 1280 1064 860 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2029 2348 3938 4535 5322 5590 1239 Size: 393 397 319 1590 597 787 268 1524 Fragments arranged by size: 1590 1524 787 597 397 393 319 268 EcoO109I rG'GnC_Cy Cuts at: 2336 2336 Size: 5875 EcoRI G'AATT_C Cuts at: 3184 3562 3184 Size: 378 5497 EcoRV GAT'ATC Cuts at: 2621 2693 2765 2837 2909 2981 3053 2621 Size: 72 72 72 72 72 72 5443 Fragments arranged by size: 5443 72 72 72 72 72 72 FspI TGC'GCA Cuts at: 4381 4381 Size: 5875 HaeII r_GCGC'y Cuts at: 1678 5022 1678 Size: 3344 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4516 4548 4590 4622 1373 Size: 32 135 32 2944 32 42 32 2626 Fragments arranged by size: 2944 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3219 3465 669 Size: 988 281 1281 246 3079 Fragments arranged by size: 3079 1281 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5875 HpaI GTT'AAC Cuts at: 3465 3465 Size: 5875 KpnI G_GTAC'C Cuts at: 3200 3200 Size: 5875 MfeI C'AATT_G Cuts at: 3474 3474 Size: 5875 MluI A'CGCG_T Cuts at: 5751 5751 Size: 5875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4989 5173 1305 Size: 3684 184 2007 Fragments arranged by size: 3684 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4053 Fragments arranged by size: 4053 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2313 3088 3136 3833 4192 4351 518 Size: 648 1147 775 48 697 359 159 2042 Fragments arranged by size: 2042 1147 775 697 648 359 159 48 MspA1I CmG'CkG Cuts at: 1606 2075 3291 3968 5116 5361 1606 Size: 469 1216 677 1148 245 2120 Fragments arranged by size: 2120 1216 1148 677 469 245 NcoI C'CATG_G Cuts at: 513 1065 2314 513 Size: 552 1249 4074 Fragments arranged by size: 4074 1249 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5875 NheI G'CTAG_C Cuts at: 1052 2081 1052 Size: 1029 4846 NotI GC'GGCC_GC Cuts at: 3322 3322 Size: 5875 NspI r_CATG'y Cuts at: 1132 1229 3233 4778 1132 Size: 97 2004 1545 2229 Fragments arranged by size: 2229 2004 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5875 PciI A'CATG_T Cuts at: 4774 4774 Size: 5875 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5875 PmeI GTTT'AAAC Cuts at: 3179 3179 Size: 5875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3944 3976 5457 5489 3944 Size: 32 1481 32 4330 Fragments arranged by size: 4330 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5875 PsiI TTA'TAA Cuts at: 3445 3445 Size: 5875 PspOMI G'GGCC_C Cuts at: 2335 2335 Size: 5875 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2179 2211 2179 Size: 32 5843 PstI C_TGCA'G Cuts at: 830 2080 2463 3227 830 Size: 1250 383 764 3478 Fragments arranged by size: 3478 1250 764 383 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5875 PvuII CAG'CTG Cuts at: 2075 2075 Size: 5875 SalI G'TCGA_C Cuts at: 1936 3217 1936 Size: 1281 4594 SbfI CC_TGCA'GG Cuts at: 3227 3227 Size: 5875 ScaI AGT'ACT Cuts at: 4123 5835 4123 Size: 1712 4163 SexAI A'CCwGG_T Cuts at: 2527 2527 Size: 5875 SfcI C'TryA_G Cuts at: 826 1045 2076 2459 3223 4358 5039 5230 826 Size: 219 1031 383 764 1135 681 191 1471 Fragments arranged by size: 1471 1135 1031 764 681 383 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5875 SmaI CCC'GGG Cuts at: 1872 3202 1872 Size: 1330 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3979 4880 5142 820 Size: 197 926 2036 901 262 1553 Fragments arranged by size: 2036 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3666 493 Size: 3173 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5875 SphI G_CATG'C Cuts at: 1132 3233 1132 Size: 2101 3774 SspI AAT'ATT Cuts at: 5 52 3799 5 Size: 47 3747 2081 Fragments arranged by size: 3747 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5875 StyI C'CwwG_G Cuts at: 513 1065 2086 2314 3314 513 Size: 552 1021 228 1000 3074 Fragments arranged by size: 3074 1021 1000 552 228 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2075 2083 3140 3902 3919 4072 4257 Size: 1255 495 8 1057 762 17 153 185 Cuts at: 4257 4702 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1057 762 495 445 185 153 17 8 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4121 5833 96 Size: 274 80 33 51 1443 2144 1712 138 Fragments arranged by size: 2144 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2464 2496 5835 5867 2464 Size: 32 3339 32 2472 Fragments arranged by size: 3339 2472 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5875 XbaI T'CTAG_A Cuts at: 3211 3211 Size: 5875 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5875 XmaI C'CCGG_G Cuts at: 1870 3200 1870 Size: 1330 4545 XmnI GAAnn'nnTTC Cuts at: 4004 4004 Size: 5875 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3683 276 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI