################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbsI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaI BseAI BseRI BsiHKAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI Ksp632I KspI MamI MboII MfeI MluI MluNI MroI MscI MseI MslI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I TspRI Tth111I Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### Acc65I 194 GGTACC G^GTACC AccII 191 CGCG CG^CG AcyI 34 GGCGCC G[GA]^CG[CT]C AgeI 87 ACCGGT A^CCGGT AlwNI 133 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 356 GGGCCC GGGCC^C Asp718I 194 GGTACC G^GTACC BbeI 34 GGCGCC GGCGC^C BbvCI 472 GCTGAGG CCTCAGC(-5/-2) BcgI 180 CGAAGCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 93 CCCGG CC^[GC]GG BfaI 230 CTAG C^TAG BsaAI 169 CACGTA [CT]AC^GT[GA] BsaHI 34 GGCGCC G[GA]^CG[CT]C BsaWI 87 ACCGGT [AT]^CCGG[AT] BsgI 220 GTGCAG GTGCAG(16/14) BsiEI 85 CGACCG CG[GA][CT]^CG BsiWI 171 CGTACG C^GTACG BsmAI 14 GAGAC GTCTC(1/5) BspMI 348 GCAGGT ACCTGC(4/8) BsrFI 87 ACCGGT [GA]^CCGG[CT] BsrI 484 CCAGT ACTGG(1/-1) BssSI 9 CACGAG CACGAG(-5/-1) BstUI 191 CGCG CG^CG BstYI 404 AGATCC [GA]^GATC[CT] Cfr10I 87 ACCGGT [GA]^CCGG[CT] DsaI 56 CCATGG C^C[GA][CT]GG EcoNI 468 CCTTGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 56 CCATGG C^C[AT][AT]GG HaeII 34 GGCGCC [GA]GCGC^[CT] HgaI 397 GACGC GACGC(5/10) Hin1I 34 GGCGCC G[GA]^CG[CT]C HphI 351 GGTGA GGTGA(8/7) KasI 34 GGCGCC G^GCGCC KpnI 194 GGTACC GGTAC^C MaeI 230 CTAG C^TAG MflI 404 AGATCC [GA]^GATC[CT] MvnI 191 CGCG CG^CG NarI 34 GGCGCC GG^CGCC NciI 93 CCCGG CC^[GC]GG NcoI 56 CCATGG C^CATGG PaeR7I 154 CTCGAG C^TCGAG PinAI 87 ACCGGT A^CCGGT PpuMI 308 GGGTCCT [GA]G^G[AT]CC[CT] PstI 160 CTGCAG CTGCA^G SfaNI 416 GCATC GCATC(5/9) SfcI 160 CTGCAG C^T[GA][CT]AG SfoI 34 GGCGCC GGC^GCC SmlI 154 CTCGAG C^T[CT][GA]AG StuI 465 AGGCCT AGG^CCT StyI 56 CCATGG C^C[AT][AT]GG TaqI 155 TCGA T^CGA TthHB8I 155 TCGA T^CGA XcmI 47 CCATCCTCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 154 CTCGAG C^TCGAG XhoII 404 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AluI 158 AGCT AG^CT 333 AGCT AlwI 405 GATCC GGATC(4/5) 429 GATCC AocI 282 CCTGAGG CC^T[ACGT]AGG 434 CCTTAGG AvaI 154 CTCGAG C^[CT]CG[GA]G 359 CCCGAG BanI 34 GGCGCC G^G[CT][GA]CC 194 GGTACC BanII 4 GAGCCC G[GA]GC[CT]^C 356 GGGCCC BmyI 4 GAGCCC G[AGT]GC[ACT]^C 356 GGGCCC BsaJI 56 CCATGG C^C[ACGT][ACGT]GG 305 CCTGGG BsmFI 395 GGGAC GGGAC(10/14) 91 GTCCC BsoBI 154 CTCGAG C^[CT]CG[GA]G 359 CCCGAG Bsp1286I 4 GAGCCC G[AGT]GC[ACT]^C 356 GGGCCC Bsu36I 282 CCTGAGG CC^T[ACGT]AGG 434 CCTTAGG CfoI 35 GCGC GCG^C 190 GCGC DpnII 405 GATC ^GATC 429 GATC DraII 308 GGGTCCT [GA]G^G[ACGT]CC[CT] 355 AGGGCCC Eco81I 282 CCTGAGG CC^T[ACGT]AGG 434 CCTTAGG EcoO109I 308 GGGTCCT [GA]G^G[ACGT]CC[CT] 355 AGGGCCC FauI 21 CCCGC CCCGC(4/6) 392 GCGGG HapII 88 CCGG C^CGG 94 CCGG HhaI 35 GCGC GCG^C 190 GCGC HinP1I 35 GCGC G^CGC 190 GCGC HinfI 247 GAGTC G^A[ACGT]TC 423 GACTC HpaII 88 CCGG C^CGG 94 CCGG MaeIII 124 GTGAC ^GT[ACGT]AC 452 GTGAC MboI 405 GATC ^GATC 429 GATC MspA1I 22 CCGCTG C[AC]G^C[GT]G 291 CCGCTG MspI 88 CCGG C^CGG 94 CCGG MwoI 37 GCCTTTTCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 472 GCTGAGGCTGC NdeII 405 GATC ^GATC 429 GATC NlaIII 57 CATG CATG^ 241 CATG PleI 423 GACTC GAGTC(4/5) 247 GAGTC Sau3AI 405 GATC ^GATC 429 GATC Tsp45I 124 GTGAC ^GT[GC]AC 452 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AciI AfaI AvaII BbvI BsiYI BslI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII ItaI MaeII MnlI MvaI NlaIV RsaI Sau96I ScrFI TseI