(Circular) (Six-base) MAPSORT of: pFN21AE6560.seq Check: 6671 from: 1 to: 5200 pFN21AE6560 5200 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2344 2344 Size: 5200 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3010 278 Size: 53 83 186 936 1474 2468 Fragments arranged by size: 2468 1474 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2543 1937 Size: 606 4594 Acc65I G'GTAC_C Cuts at: 2199 2521 2199 Size: 322 4878 AclI AA'CG_TT Cuts at: 1563 3327 3700 1563 Size: 1764 373 3063 Fragments arranged by size: 3063 1764 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3263 4647 4915 1632 Size: 1631 1384 268 1917 Fragments arranged by size: 1917 1631 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5003 AflIII A'CryG_T Cuts at: 1203 4099 5076 1203 Size: 2896 977 1327 Fragments arranged by size: 2896 1327 977 AgeI A'CCGG_T Cuts at: 2092 4031 2092 Size: 1939 3261 AhdI GACnn_n'nnGTC Cuts at: 3929 3929 Size: 5200 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5200 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5200 AlwNI CAG_nnn'CTG Cuts at: 2143 2943 4515 2143 Size: 800 1572 2828 Fragments arranged by size: 2828 1572 800 ApaI G_GGCC'C Cuts at: 2365 2365 Size: 5200 ApaLI G'TGCA_C Cuts at: 3257 4413 3257 Size: 1156 4044 ApoI r'AATT_y Cuts at: 1493 2509 2735 2887 4996 1493 Size: 1016 226 152 2109 1697 Fragments arranged by size: 2109 1697 1016 226 152 AseI AT'TA_AT Cuts at: 160 1032 3754 160 Size: 872 2722 1606 Fragments arranged by size: 2722 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5200 AvaI C'yCGr_G Cuts at: 1870 1943 2159 2364 2525 1870 Size: 73 216 205 161 4545 Fragments arranged by size: 4545 216 205 161 73 BamHI G'GATC_C Cuts at: 2530 2897 2530 Size: 367 4833 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 2199 2521 3976 618 Size: 325 217 627 252 160 322 1455 1842 Fragments arranged by size: 1842 1455 627 325 322 252 217 160 BanII G_rGCy'C Cuts at: 1391 2013 2365 2519 1391 Size: 622 352 154 4072 Fragments arranged by size: 4072 622 352 154 BbeI G_GCGC'C Cuts at: 2043 2043 Size: 5200 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4229 BbvCI CC'TCA_GC Cuts at: 2478 2478 Size: 5200 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2174 2208 3391 3425 1903 Size: 34 237 34 1183 34 3678 Fragments arranged by size: 3678 1183 237 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3092 4302 3092 Size: 1210 3990 BclI T'GATC_A Cuts at: 1548 1548 Size: 5200 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3811 136 Size: 107 122 71 631 605 2139 1525 Fragments arranged by size: 2139 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5195 5195 Size: 5200 BlpI GC'TnA_GC Cuts at: 2617 2617 Size: 5200 Bme1580I G_kGCm'C Cuts at: 1790 2365 3261 4417 1790 Size: 575 896 1156 2573 Fragments arranged by size: 2573 1156 896 575 BmgBI CAC'GTC Cuts at: 1318 2456 1318 Size: 1138 4062 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2483 2931 3889 Size: 416 53 83 399 24 1066 448 958 Cuts at: 3889 442 Size: 1753 Fragments arranged by size: 1753 1066 958 448 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5200 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3860 1239 Size: 2621 2579 Bpu10I CC'TnA_GC Cuts at: 2340 2478 2340 Size: 138 5062 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3325 4190 4488 3325 Size: 865 298 4037 Fragments arranged by size: 4037 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3863 882 Size: 617 2364 2219 Fragments arranged by size: 2364 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2176 2991 493 Size: 711 972 815 2702 Fragments arranged by size: 2702 972 815 711 BsaBI GATnn'nnATC Cuts at: 5194 5194 Size: 5200 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2040 3007 3389 4024 Size: 53 83 186 936 507 967 382 635 Cuts at: 4024 275 Size: 1451 Fragments arranged by size: 1451 967 936 635 507 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2092 3633 4031 4305 4452 4863 2092 Size: 1541 398 274 147 411 2429 Fragments arranged by size: 2429 1541 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4975 Fragments arranged by size: 4975 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5200 BseYI C'CCAG_C Cuts at: 4403 4899 4403 Size: 496 4704 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2246 1163 Size: 573 510 4117 Fragments arranged by size: 4117 573 510 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2093 2650 3411 4439 1540 Size: 275 186 92 557 761 1028 2301 Fragments arranged by size: 2301 1028 761 557 275 186 92 BsiHKAI G_wGCw'C Cuts at: 3261 3346 4417 3261 Size: 85 1071 4044 Fragments arranged by size: 4044 1071 85 BsiWI C'GTAC_G Cuts at: 2176 2176 Size: 5200 BsmI GAATG_Cn' Cuts at: 2711 2804 2711 Size: 93 5107 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2365 2519 3261 3346 4417 1391 Size: 399 223 352 154 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 352 223 154 85 BspEI T'CCGG_A Cuts at: 4863 4863 Size: 5200 BspHI T'CATG_A Cuts at: 3089 4819 3089 Size: 1730 3470 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2344 2555 844 Size: 1500 211 3489 Fragments arranged by size: 3489 1500 211 BsrBI CCG'CTC Cuts at: 1394 1823 3087 1394 Size: 429 1264 3507 Fragments arranged by size: 3507 1264 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3695 3869 65 Size: 1183 2447 174 1396 Fragments arranged by size: 2447 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2092 3844 4031 1700 Size: 110 282 1752 187 2869 Fragments arranged by size: 2869 1752 282 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5200 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5200 BssSI C'ACGA_G Cuts at: 2014 3260 4272 2014 Size: 1246 1012 2942 Fragments arranged by size: 2942 1246 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5200 BstBI TT'CG_AA Cuts at: 4004 4004 Size: 5200 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5200 BstYI r'GATC_y Cuts at: 1972 2409 2530 2897 3282 3299 4751 4837 4849 Size: 437 121 367 385 17 1452 86 12 Cuts at: 4849 5195 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 437 385 367 346 121 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2288 2440 2288 Size: 152 5048 BtgI C'CryG_G Cuts at: 513 1065 2004 2061 513 Size: 552 939 57 3652 Fragments arranged by size: 3652 939 552 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4461 Fragments arranged by size: 4461 683 56 BtsI GCAGTG_nn' Cuts at: 2716 3510 3530 2716 Size: 794 20 4386 Fragments arranged by size: 4386 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5200 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5165 DraI TTT'AAA Cuts at: 2504 2504 Size: 5200 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4207 809 Size: 507 2891 1802 Fragments arranged by size: 2891 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2647 3536 5165 Size: 54 1425 50 275 18 817 889 1629 Cuts at: 5165 8 Size: 43 Fragments arranged by size: 1629 1425 889 817 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2647 1537 Size: 275 835 4090 Fragments arranged by size: 4090 835 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3130 1353 Size: 1777 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2038 3787 4161 4307 387 Size: 1064 138 449 1749 374 146 1280 Fragments arranged by size: 1749 1280 1064 449 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3263 3860 4647 4915 1239 Size: 393 1631 597 787 268 1524 Fragments arranged by size: 1631 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2477 2477 Size: 5200 EcoO109I rG'GnC_Cy Cuts at: 2314 2361 2314 Size: 47 5153 EcoRI G'AATT_C Cuts at: 2509 2887 2509 Size: 378 4822 FspI TGC'GCA Cuts at: 3706 3706 Size: 5200 HaeII r_GCGC'y Cuts at: 1678 2043 4347 1678 Size: 365 2304 2531 Fragments arranged by size: 2531 2304 365 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2450 2482 3841 3873 3915 Size: 32 135 32 878 32 1359 32 42 Cuts at: 3915 3947 1373 Size: 32 2626 Fragments arranged by size: 2626 1359 878 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2544 2790 669 Size: 988 281 606 246 3079 Fragments arranged by size: 3079 988 606 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5200 HpaI GTT'AAC Cuts at: 2790 2790 Size: 5200 KasI G'GCGC_C Cuts at: 2039 2039 Size: 5200 KpnI G_GTAC'C Cuts at: 2203 2525 2203 Size: 322 4878 MfeI C'AATT_G Cuts at: 2799 2799 Size: 5200 MluI A'CGCG_T Cuts at: 5076 5076 Size: 5200 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2113 4314 4498 1305 Size: 808 2201 184 2007 Fragments arranged by size: 2201 2007 808 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3378 Fragments arranged by size: 3378 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3158 3517 3676 518 Size: 648 1992 359 159 2042 Fragments arranged by size: 2042 1992 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2029 2298 2616 3293 4441 4686 1606 Size: 423 269 318 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 423 318 269 245 NarI GG'CG_CC Cuts at: 2040 2040 Size: 5200 NcoI C'CATG_G Cuts at: 513 1065 2004 2061 513 Size: 552 939 57 3652 Fragments arranged by size: 3652 939 552 57 NdeI CA'TA_TG Cuts at: 387 387 Size: 5200 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5200 NotI GC'GGCC_GC Cuts at: 2647 2647 Size: 5200 NspI r_CATG'y Cuts at: 1132 1229 2558 4103 1132 Size: 97 1329 1545 2229 Fragments arranged by size: 2229 1545 1329 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5200 PciI A'CATG_T Cuts at: 4099 4099 Size: 5200 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5200 PfoI T'CCnGG_A Cuts at: 2097 2381 2097 Size: 284 4916 PmeI GTTT'AAAC Cuts at: 2504 2504 Size: 5200 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3269 3301 4782 4814 3269 Size: 32 1481 32 3655 Fragments arranged by size: 3655 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2314 2314 Size: 5200 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5200 PsiI TTA'TAA Cuts at: 2770 2770 Size: 5200 PspOMI G'GGCC_C Cuts at: 2361 2361 Size: 5200 PstI C_TGCA'G Cuts at: 830 2169 2552 830 Size: 1339 383 3478 Fragments arranged by size: 3478 1339 383 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5200 SalI G'TCGA_C Cuts at: 1936 2542 1936 Size: 606 4594 SbfI CC_TGCA'GG Cuts at: 2552 2552 Size: 5200 ScaI AGT'ACT Cuts at: 3448 5160 3448 Size: 1712 3488 SfcI C'TryA_G Cuts at: 826 1045 2165 2548 3683 4364 4555 826 Size: 219 1120 383 1135 681 191 1471 Fragments arranged by size: 1471 1135 1120 681 383 219 191 SfoI GGC'GCC Cuts at: 2041 2041 Size: 5200 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5200 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5200 SmaI CCC'GGG Cuts at: 1872 2527 1872 Size: 655 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2159 3304 4205 4467 820 Size: 197 926 216 1145 901 262 1553 Fragments arranged by size: 1553 1145 926 901 262 216 197 SnaBI TAC'GTA Cuts at: 493 2991 493 Size: 2498 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5200 SphI G_CATG'C Cuts at: 1132 2558 1132 Size: 1426 3774 SspI AAT'ATT Cuts at: 5 52 3124 5 Size: 47 3072 2081 Fragments arranged by size: 3072 2081 47 StuI AGG'CCT Cuts at: 1517 2472 1517 Size: 955 4245 StyI C'CwwG_G Cuts at: 513 1065 2004 2061 2639 513 Size: 552 939 57 578 3074 Fragments arranged by size: 3074 939 578 552 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3227 3244 3397 3582 4027 325 Size: 1255 1647 17 153 185 445 1498 Fragments arranged by size: 1647 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3446 5158 96 Size: 274 80 33 51 1443 1469 1712 138 Fragments arranged by size: 1712 1469 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5160 5192 5160 Size: 32 5168 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5200 XbaI T'CTAG_A Cuts at: 2536 2536 Size: 5200 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2059 2059 Size: 5200 XhoI C'TCGA_G Cuts at: 1943 2159 1943 Size: 216 4984 XmaI C'CCGG_G Cuts at: 1870 2525 1870 Size: 655 4545 XmnI GAAnn'nnTTC Cuts at: 3329 3329 Size: 5200 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3008 276 Size: 53 83 186 936 1474 2468 Fragments arranged by size: 2468 1474 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI