Restriction map for
pFN21AE6560
(Linear) (Six-base) MAPSORT of: KIEE6560.seq Check: 7234 from: 1 to: 495
KIEE6560.orf
With 182 enzymes: SgfI *
March 16, 2015 11:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 339 495
Size: 339 156
Acc65I G'GTAC_C
Cuts at: 0 194 495
Size: 194 301
AgeI A'CCGG_T
Cuts at: 0 87 495
Size: 87 408
AlwNI CAG_nnn'CTG
Cuts at: 0 138 495
Size: 138 357
ApaI G_GGCC'C
Cuts at: 0 360 495
Size: 360 135
AvaI C'yCGr_G
Cuts at: 0 154 359 495
Size: 154 205 136
Fragments arranged by size:
205 154 136
BanI G'GyrC_C
Cuts at: 0 34 194 495
Size: 34 160 301
Fragments arranged by size:
301 160 34
BanII G_rGCy'C
Cuts at: 0 8 360 495
Size: 8 352 135
Fragments arranged by size:
352 135 8
BbeI G_GCGC'C
Cuts at: 0 38 495
Size: 38 457
BbvCI CC'TCA_GC
Cuts at: 0 473 495
Size: 473 22
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 169 203 495
Size: 169 34 292
Fragments arranged by size:
292 169 34
Bme1580I G_kGCm'C
Cuts at: 0 360 495
Size: 360 135
BmgBI CAC'GTC
Cuts at: 0 451 495
Size: 451 44
BmrI ACTGGGnnnn_n'
Cuts at: 0 478 495
Size: 478 17
Bpu10I CC'TnA_GC
Cuts at: 0 335 473 495
Size: 335 138 22
Fragments arranged by size:
335 138 22
BsaAI yAC'GTr
Cuts at: 0 171 495
Size: 171 324
BsaHI Gr'CG_yC
Cuts at: 0 35 495
Size: 35 460
BsaWI w'CCGG_w
Cuts at: 0 87 495
Size: 87 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 495
Size: 241 254
BsiEI CG_ry'CG
Cuts at: 0 88 495
Size: 88 407
BsiWI C'GTAC_G
Cuts at: 0 171 495
Size: 171 324
Bsp1286I G_dGCh'C
Cuts at: 0 8 360 495
Size: 8 352 135
Fragments arranged by size:
352 135 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 339 495
Size: 339 156
BsrFI r'CCGG_y
Cuts at: 0 87 495
Size: 87 408
BssSI C'ACGA_G
Cuts at: 0 9 495
Size: 9 486
BstYI r'GATC_y
Cuts at: 0 404 495
Size: 404 91
Bsu36I CC'TnA_GG
Cuts at: 0 283 435 495
Size: 283 152 60
Fragments arranged by size:
283 152 60
BtgI C'CryG_G
Cuts at: 0 56 495
Size: 56 439
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 33 495
Size: 33 462
EcoNI CCTnn'n_nnAGG
Cuts at: 0 472 495
Size: 472 23
EcoO109I rG'GnC_Cy
Cuts at: 0 309 356 495
Size: 309 47 139
Fragments arranged by size:
309 139 47
HaeII r_GCGC'y
Cuts at: 0 38 495
Size: 38 457
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 445 477 495
Size: 445 32 18
Fragments arranged by size:
445 32 18
KasI G'GCGC_C
Cuts at: 0 34 495
Size: 34 461
KpnI G_GTAC'C
Cuts at: 0 198 495
Size: 198 297
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 495
Size: 108 387
MspA1I CmG'CkG
Cuts at: 0 24 293 495
Size: 24 269 202
Fragments arranged by size:
269 202 24
NarI GG'CG_CC
Cuts at: 0 35 495
Size: 35 460
NcoI C'CATG_G
Cuts at: 0 56 495
Size: 56 439
PfoI T'CCnGG_A
Cuts at: 0 92 376 495
Size: 92 284 119
Fragments arranged by size:
284 119 92
PpuMI rG'GwC_Cy
Cuts at: 0 309 495
Size: 309 186
PspOMI G'GGCC_C
Cuts at: 0 356 495
Size: 356 139
PstI C_TGCA'G
Cuts at: 0 164 495
Size: 164 331
SfcI C'TryA_G
Cuts at: 0 160 495
Size: 160 335
SfoI GGC'GCC
Cuts at: 0 36 495
Size: 36 459
SmlI C'TyrA_G
Cuts at: 0 154 495
Size: 154 341
StuI AGG'CCT
Cuts at: 0 467 495
Size: 467 28
StyI C'CwwG_G
Cuts at: 0 56 495
Size: 56 439
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 54 495
Size: 54 441
XhoI C'TCGA_G
Cuts at: 0 154 495
Size: 154 341
Enzymes that do cut:
AarI Acc65I AgeI AlwNI ApaI AvaI BanI BanII
BbeI BbvCI BcgI Bme1580I BmgBI BmrI Bpu10I BsaAI
BsaHI BsaWI BsgI BsiEI BsiWI Bsp1286I BspMI BsrFI
BssSI BstYI Bsu36I BtgI EciI EcoNI EcoO109I HaeII
Hin4I KasI KpnI MmeI MspA1I NarI NcoI PfoI
PpuMI PspOMI PstI SfcI SfoI SmlI StuI StyI
XcmI XhoI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BciVI BclI BfrBI BglI
BglII BlpI BmtI BplI BpmI BpuEI BsaI BsaBI
BsaXI BseRI BseYI BsiHKAI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EcoICRI Eco57MI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI MfeI MluI
MscI MslI NaeI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TatI TstI Tth111I XbaI XmaI
XmnI ZraI