Restriction map for
pFN21AE6185
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsiYI BslI BsmAI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DdeI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HaeIII HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MluNI MroI MscI MslI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 26 GTCGAC GT^[AC][GT]AC
AcsI 365 AAATTC [GA]^AATT[CT]
AfaI 57 GTAC GT^AC
ApoI 365 AAATTC [GA]^AATT[CT]
Asp700I 100 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 229 GAGCTC G[AT]GC[AT]^C
AvaI 95 CCCGGG C^[CT]CG[GA]G
BbsI 406 GAAGAC GAAGAC(2/6)
BbvI 267 GCTGC GCAGC(8/12)
BglI 440 GCCCCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 406 GAAGAC GAAGAC(2/6)
BsaAI 259 TACGTG [CT]AC^GT[GA]
BsiHKAI 229 GAGCTC G[AT]GC[AT]^C
BsoBI 95 CCCGGG C^[CT]CG[GA]G
BsrDI 478 CATTGC GCAATG(2/0)
BsrI 152 ACTGG ACTGG(1/-1)
BstAPI 449 GCAGAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 339 CCTGG CC^[AT]GG
DsaI 123 CCGTGG C^C[GA][CT]GG
EarI 19 CTCTTC CTCTTC(1/4)
EcoRII 339 CCTGG ^CC[AT]GG
Fnu4HI 267 GCTGC GC^[ACGT]GC
HapII 96 CCGG C^CGG
HgaI 237 GACGC GACGC(5/10)
HpaII 96 CCGG C^CGG
HphI 92 TCACC GGTGA(8/7)
ItaI 267 GCTGC GC^[ACGT]GC
Ksp632I 19 CTCTTC CTCTTC(1/4)
MaeII 260 ACGT A^CGT
MseI 314 TTAA T^TAA
MspI 96 CCGG C^CGG
MvaI 339 CCTGG CC^[AT]GG
NlaIII 473 CATG CATG^
PleI 409 GACTC GAGTC(4/5)
RsaI 57 GTAC GT^AC
SacI 229 GAGCTC GAGCT^C
SalI 26 GTCGAC G^TCGAC
SapI 18 GCTCTTC GCTCTTC(1/4)
SfaNI 252 GCATC GCATC(5/9)
SfcI 46 CTACAG C^T[GA][CT]AG
SmaI 95 CCCGGG CCC^GGG
SspI 278 AATATT AAT^ATT
TaqI 27 TCGA T^CGA
TfiI 429 GAATC G^A[AT]TC
Tru9I 314 TTAA T^TAA
TseI 267 GCTGC G^C[AT]GC
Tsp45I 128 GTCAC ^GT[GC]AC
TspRI 459 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 27 TCGA T^CGA
XmaCI 95 CCCGGG C^CCGGG
XmaI 95 CCCGGG C^CCGGG
XmnI 100 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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BanII 229 GAGCTC G[GA]GC[CT]^C
438 GAGCCC
BcnI 95 CCCGG CC^[GC]GG
96 CCGGG
BmyI 229 GAGCTC G[AGT]GC[ACT]^C
438 GAGCCC
BsaJI 95 CCCGGG C^C[ACGT][ACGT]GG
123 CCGTGG
Bsp1286I 229 GAGCTC G[AGT]GC[ACT]^C
438 GAGCCC
BstF5I 336 CATCC GGATG(2/0)
377 CATCC
DpnII 90 GATC ^GATC
470 GATC
FokI 336 CATCC GGATG(9/13)
377 CATCC
HincII 26 GTCGAC GT[CT]^[GA]AC
148 GTTGAC
HindII 26 GTCGAC GT[CT]^[GA]AC
148 GTTGAC
HinfI 409 GACTC G^A[ACGT]TC
429 GAATC
MboI 90 GATC ^GATC
470 GATC
MnlI 81 GAGG CCTC(7/6)
446 GAGG
NciI 95 CCCGG CC^[GC]GG
96 CCGGG
NdeII 90 GATC ^GATC
470 GATC
Sau3AI 90 GATC ^GATC
470 GATC
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
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AluI BfaI BssKI MaeI MaeIII
MboII MwoI ScrFI Tsp509I