(Circular) (Six-base) MAPSORT of: pFN21AE6185.seq Check: 2821 from: 1 to: 5197 pFN21AE6185 5197 bp With 182 enzymes: SgfI * December 8, 2014 13:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3007 278 Size: 53 83 186 936 1471 2468 Fragments arranged by size: 2468 1471 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2032 2540 1937 Size: 95 508 4594 Fragments arranged by size: 4594 508 95 Acc65I G'GTAC_C Cuts at: 2518 2518 Size: 5197 AclI AA'CG_TT Cuts at: 1563 3324 3697 1563 Size: 1761 373 3063 Fragments arranged by size: 3063 1761 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2011 3260 4644 4912 1632 Size: 379 1249 1384 268 1917 Fragments arranged by size: 1917 1384 1249 379 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5000 AflIII A'CryG_T Cuts at: 1203 4096 5073 1203 Size: 2893 977 1327 Fragments arranged by size: 2893 1327 977 AgeI A'CCGG_T Cuts at: 4028 4028 Size: 5197 AhdI GACnn_n'nnGTC Cuts at: 3926 3926 Size: 5197 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5197 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5197 AlwNI CAG_nnn'CTG Cuts at: 2940 4512 2940 Size: 1572 3625 ApaLI G'TGCA_C Cuts at: 3254 4410 3254 Size: 1156 4041 ApoI r'AATT_y Cuts at: 1493 2370 2506 2732 2884 4993 1493 Size: 877 136 226 152 2109 1697 Fragments arranged by size: 2109 1697 877 226 152 136 AseI AT'TA_AT Cuts at: 160 1032 3751 160 Size: 872 2719 1606 Fragments arranged by size: 2719 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5197 AvaI C'yCGr_G Cuts at: 1870 1943 2100 2522 1870 Size: 73 157 422 4545 Fragments arranged by size: 4545 422 157 73 BamHI G'GATC_C Cuts at: 2527 2894 2527 Size: 367 4830 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2518 3973 618 Size: 325 217 627 731 1455 1842 Fragments arranged by size: 1842 1455 731 627 325 217 BanII G_rGCy'C Cuts at: 1391 2238 2447 2516 1391 Size: 847 209 69 4072 Fragments arranged by size: 4072 847 209 69 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2418 928 Size: 971 519 3707 Fragments arranged by size: 3707 971 519 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3388 3422 1903 Size: 34 1451 34 3678 Fragments arranged by size: 3678 1451 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3089 4299 3089 Size: 1210 3987 BclI T'GATC_A Cuts at: 1548 1548 Size: 5197 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2451 3808 136 Size: 107 122 71 631 605 779 1357 1525 Fragments arranged by size: 1525 1357 779 631 605 122 107 71 BglII A'GATC_T Cuts at: 5192 5192 Size: 5197 BlpI GC'TnA_GC Cuts at: 2614 2614 Size: 5197 Bme1580I G_kGCm'C Cuts at: 1790 3258 4414 1790 Size: 1468 1156 2573 Fragments arranged by size: 2573 1468 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5197 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2928 3886 442 Size: 416 53 83 399 24 1511 958 1753 Fragments arranged by size: 1753 1511 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5197 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3857 1239 Size: 2618 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3322 4187 4485 3322 Size: 865 298 4034 Fragments arranged by size: 4034 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3860 882 Size: 617 2361 2219 Fragments arranged by size: 2361 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2266 2988 493 Size: 711 1062 722 2702 Fragments arranged by size: 2702 1062 722 711 BsaBI GATnn'nnATC Cuts at: 5191 5191 Size: 5197 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3004 3386 4021 275 Size: 53 83 186 936 1471 382 635 1451 Fragments arranged by size: 1471 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3630 4028 4302 4449 4860 3630 Size: 398 274 147 411 3967 Fragments arranged by size: 3967 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4972 Fragments arranged by size: 4972 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5197 BseYI C'CCAG_C Cuts at: 4400 4896 4400 Size: 496 4701 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4624 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2647 3408 4436 1540 Size: 275 186 646 761 1028 2301 Fragments arranged by size: 2301 1028 761 646 275 186 BsiHKAI G_wGCw'C Cuts at: 2238 3258 3343 4414 2238 Size: 1020 85 1071 3021 Fragments arranged by size: 3021 1071 1020 85 BsmI GAATG_Cn' Cuts at: 2708 2801 2708 Size: 93 5104 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2238 2447 2516 3258 3343 4414 1391 Size: 399 448 209 69 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 448 399 209 85 69 BspEI T'CCGG_A Cuts at: 4860 4860 Size: 5197 BspHI T'CATG_A Cuts at: 3086 4816 3086 Size: 1730 3467 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2552 844 Size: 1708 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3084 1394 Size: 429 1261 3507 Fragments arranged by size: 3507 1261 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2482 3692 3866 65 Size: 1183 1234 1210 174 1396 Fragments arranged by size: 1396 1234 1210 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3841 4028 1700 Size: 110 2031 187 2869 Fragments arranged by size: 2869 2031 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5197 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5197 BssSI C'ACGA_G Cuts at: 3257 4269 3257 Size: 1012 4185 BstAPI GCAn_nnn'nTGC Cuts at: 794 2460 794 Size: 1666 3531 BstBI TT'CG_AA Cuts at: 4001 4001 Size: 5197 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5197 BstYI r'GATC_y Cuts at: 1972 2527 2894 3279 3296 4748 4834 4846 5192 Size: 555 367 385 17 1452 86 12 346 Cuts at: 5192 1972 Size: 1977 Fragments arranged by size: 1977 1452 555 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2128 513 Size: 552 1063 3582 Fragments arranged by size: 3582 1063 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4458 Fragments arranged by size: 4458 683 56 BtsI GCAGTG_nn' Cuts at: 2470 2713 3507 3527 2470 Size: 243 794 20 4140 Fragments arranged by size: 4140 794 243 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5197 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5162 DraI TTT'AAA Cuts at: 2501 2501 Size: 5197 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4204 809 Size: 507 2888 1802 Fragments arranged by size: 2888 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2644 3533 5162 Size: 54 1425 50 275 18 814 889 1629 Cuts at: 5162 8 Size: 43 Fragments arranged by size: 1629 1425 889 814 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2644 1537 Size: 275 832 4090 Fragments arranged by size: 4090 832 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2030 3127 1353 Size: 677 1097 3423 Fragments arranged by size: 3423 1097 677 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3784 4158 4304 387 Size: 1064 138 2195 374 146 1280 Fragments arranged by size: 2195 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2236 2236 Size: 5197 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2011 3260 3857 4644 4912 1239 Size: 393 379 1249 597 787 268 1524 Fragments arranged by size: 1524 1249 787 597 393 379 268 EcoRI G'AATT_C Cuts at: 2506 2884 2506 Size: 378 4819 FspI TGC'GCA Cuts at: 3703 3703 Size: 5197 HaeII r_GCGC'y Cuts at: 1678 4344 1678 Size: 2666 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2242 2274 2459 2491 3838 Size: 32 135 32 670 32 185 32 1347 Cuts at: 3838 3870 3912 3944 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1347 670 185 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2033 2155 2541 2787 669 Size: 988 281 95 122 386 246 3079 Fragments arranged by size: 3079 988 386 281 246 122 95 HindIII A'AGCT_T Cuts at: 748 748 Size: 5197 HpaI GTT'AAC Cuts at: 2787 2787 Size: 5197 KpnI G_GTAC'C Cuts at: 2522 2522 Size: 5197 MfeI C'AATT_G Cuts at: 2796 2796 Size: 5197 MluI A'CGCG_T Cuts at: 5073 5073 Size: 5197 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4311 4495 1305 Size: 3006 184 2007 Fragments arranged by size: 3006 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3375 Fragments arranged by size: 3375 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3155 3514 3673 518 Size: 648 1989 359 159 2042 Fragments arranged by size: 2042 1989 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2613 3290 4438 4683 1606 Size: 1007 677 1148 245 2120 Fragments arranged by size: 2120 1148 1007 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4645 NdeI CA'TA_TG Cuts at: 387 387 Size: 5197 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5197 NotI GC'GGCC_GC Cuts at: 2644 2644 Size: 5197 NspI r_CATG'y Cuts at: 1132 1229 2555 4100 1132 Size: 97 1326 1545 2229 Fragments arranged by size: 2229 1545 1326 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5197 PciI A'CATG_T Cuts at: 4096 4096 Size: 5197 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5197 PfoI T'CCnGG_A Cuts at: 2343 2343 Size: 5197 PmeI GTTT'AAAC Cuts at: 2501 2501 Size: 5197 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3266 3298 4779 4811 3266 Size: 32 1481 32 3652 Fragments arranged by size: 3652 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5197 PsiI TTA'TAA Cuts at: 2181 2357 2767 2181 Size: 176 410 4611 Fragments arranged by size: 4611 410 176 PstI C_TGCA'G Cuts at: 830 2549 830 Size: 1719 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5197 SacI G_AGCT'C Cuts at: 2238 2238 Size: 5197 SalI G'TCGA_C Cuts at: 1936 2031 2539 1936 Size: 95 508 4594 Fragments arranged by size: 4594 508 95 SapI GCTCTTCn'nnn_ Cuts at: 2030 2030 Size: 5197 SbfI CC_TGCA'GG Cuts at: 2549 2549 Size: 5197 ScaI AGT'ACT Cuts at: 3445 5157 3445 Size: 1712 3485 SfcI C'TryA_G Cuts at: 826 1045 2051 2545 3680 4361 4552 826 Size: 219 1006 494 1135 681 191 1471 Fragments arranged by size: 1471 1135 1006 681 494 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5197 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5197 SmaI CCC'GGG Cuts at: 1872 2102 2524 1872 Size: 230 422 4545 Fragments arranged by size: 4545 422 230 SmlI C'TyrA_G Cuts at: 820 1017 1943 3301 4202 4464 820 Size: 197 926 1358 901 262 1553 Fragments arranged by size: 1553 1358 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2988 493 Size: 2495 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5197 SphI G_CATG'C Cuts at: 1132 2555 1132 Size: 1423 3774 SspI AAT'ATT Cuts at: 5 52 2285 3121 5 Size: 47 2233 836 2081 Fragments arranged by size: 2233 2081 836 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5197 StyI C'CwwG_G Cuts at: 513 1065 2636 513 Size: 552 1571 3074 Fragments arranged by size: 3074 1571 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3224 3241 3394 3579 4024 325 Size: 1255 1644 17 153 185 445 1498 Fragments arranged by size: 1644 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3443 5155 96 Size: 274 80 33 51 1443 1466 1712 138 Fragments arranged by size: 1712 1466 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5157 5189 5157 Size: 32 5165 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5197 XbaI T'CTAG_A Cuts at: 2533 2533 Size: 5197 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5197 XmaI C'CCGG_G Cuts at: 1870 2100 2522 1870 Size: 230 422 4545 Fragments arranged by size: 4545 422 230 XmnI GAAnn'nnTTC Cuts at: 2109 3326 2109 Size: 1217 3980 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3005 276 Size: 53 83 186 936 1471 2468 Fragments arranged by size: 2468 1471 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI