################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BpuAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DpnII DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI KpnI KspI MaeI MaeIII MamI MboI MfeI MflI MluI MroI MseI MunI MvnI NaeI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### ApaI 409 GGGCCC GGGCC^C BanI 193 GGCGCC G^G[CT][GA]CC BbeI 193 GGCGCC GGCGC^C BcnI 523 CCCGG CC^[GC]GG BlpI 630 GCTGAGC GC^T[ACGT]AGC Bpu1102I 630 GCTGAGC GC^T[ACGT]AGC BsaAI 415 TACGTG [CT]AC^GT[GA] BstAPI 353 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 333 GGATG GGATG(2/0) BstXI 269 CCAGTGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 630 GCTGAGC GC^T[ACGT]AGC DsaI 105 CCACGG C^C[GA][CT]GG EarI 27 GAAGAG CTCTTC(1/4) FokI 333 GGATG GGATG(9/13) HapII 524 CCGG C^CGG HgaI 553 GACGC GACGC(5/10) HpaII 524 CCGG C^CGG HphI 429 GGTGA GGTGA(8/7) KasI 193 GGCGCC G^GCGCC Ksp632I 27 GAAGAG CTCTTC(1/4) MboII 27 GAAGA GAAGA(8/7) MspI 524 CCGG C^CGG NarI 193 GGCGCC GG^CGCC NciI 523 CCCGG CC^[GC]GG PflMI 105 CCACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 54 CTGCAG CTGCA^G PvuII 537 CAGCTG CAG^CTG SexAI 425 ACCAGGT A^CC[AT]GGT SfoI 193 GGCGCC GGC^GCC StuI 74 AGGCCT AGG^CCT Van91I 105 CCACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 389 CCACAGCAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AcyI 193 GGCGCC G[GA]^CG[CT]C 553 GACGCC BanII 409 GGGCCC G[GA]GC[CT]^C 613 GAGCCC BpmI 348 CTGGAG CTGGAG(16/14) 400 CTGGAG BsaHI 193 GGCGCC G[GA]^CG[CT]C 553 GACGCC CfoI 194 GCGC GCG^C 345 GCGC DdeI 10 CTCAG C^T[ACGT]AG 631 CTGAG HaeII 193 GGCGCC [GA]GCGC^[CT] 344 GGCGCT HhaI 194 GCGC GCG^C 345 GCGC Hin1I 193 GGCGCC G[GA]^CG[CT]C 553 GACGCC HinP1I 194 GCGC G^CGC 345 GCGC MaeII 329 ACGT A^CGT 416 ACGT MslI 228 CATGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 315 CATGCGGGTG ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AluI AspHI AvaI BalI BbvI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DraII EaeI EcoO109I EcoRII FauI Fnu4HI HaeIII ItaI MluNI MnlI MscI MspA1I MvaI MwoI NlaIII NlaIV Sau96I ScrFI SfaNI SfcI TseI TspRI